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1.
Mater Horiz ; 2024 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-38910569

RESUMO

Microenvironment regulation near the catalyst surface plays a critical role in heterogeneous electrocatalytic reactions. The local concentration of reactants and intermediates significantly affects the reaction kinetics and product selectivity. Herein, we propose an innovative strategy of utilizing the spatial confinement effect in a sandwich-structured C/Cu/C assembly to regulate kinetic mass transport during the electrocatalytic CO2 reduction reaction. The sandwich C/Cu/C assembly catalyst was successfully prepared using a simple bidirectional freezing and freeze-drying method. The sandwich structure changes the free diffusion pathway of the CO intermediate within the sandwich interlayer and helps confine CO with locally increased CO concentration near the catalyst surface, which in turn promotes C-C coupling and thus improves the reaction activity and doubles the C2 product selectivity compared to its disordered mixture counterpart. This kinetics regulation in the sandwich structure may provide a new insight into the catalyst design and inspire the understanding of the structure-performance relationship in electrocatalysis.

2.
Micromachines (Basel) ; 15(3)2024 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-38542665

RESUMO

With the advancement of micro- and nanomanufacturing technologies, electronic components and chips are increasingly being miniaturized. To automatically identify their packaging materials for ensuring the reliability of ICs, a hybrid deep learning framework termed as CNN-transformer interaction (CTI) model is designed on IC packaging images in this paper, in which several cascaded CTI blocks are designed to bidirectionally capture local and global features from the IC packaging image. Each CTI block involves a CNN branch with two designed convolutional neural networks (CNNs) for CNN local features and a transformer branch with two transformers for transformer global features and transformer local-window features. A bidirectional interaction mechanism is designed to interactively transfer the features in channel and spatial dimensions between the CNNs and transformers. Experimental results indicate that the hybrid framework can recognize three types of IC packaging materials with a good performance of 96.16% F1-score and 97.92% accuracy, which is superior to some existing deep learning methods.

3.
Int J Surg Pathol ; 32(2): 380-385, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-37229663

RESUMO

Melanotic neuroectodermal tumor of infancy (MNTI) is a rare, benign neoplasm of neural crest origin that predominantly involves the craniofacial region, involvement of the epididymis being extremely rare, with about 30 cases reported. We report an unusual case of a 5-month-old male with MNTI in the epididymis. The patient underwent orchiectomy. Half a year later, there was no sign of recurrence. Whether preoperative examination or intraoperative frozen examination, the tumor may easily be misdiagnosed as malignancy. Melanotic neuroectodermal tumor of infancy should be included in differential diagnosis in infants presenting with fast-growing scrotal swelling.


Assuntos
Tumor Neuroectodérmico Melanótico , Lactente , Masculino , Humanos , Tumor Neuroectodérmico Melanótico/diagnóstico , Tumor Neuroectodérmico Melanótico/cirurgia , Epididimo , Pelve , Diagnóstico Diferencial , Orquiectomia
4.
Sci Total Environ ; 912: 168515, 2024 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-37977390

RESUMO

The trace elements present in breast milk play a vital role in the growth and development of infants. Nevertheless, numerous studies have reported the presence of toxic metal contamination in breast milk from various countries and regions, which poses potential risks to breastfed infants. This article aimed to investigate the characteristics of trace elements in breast milk and explore the relationship between breast milk and diet in Dalian, a coastal city in northeastern China. Breast milk samples and representative local food samples were collected from Dalian for research. The results revealed that 57 % of breast milk samples significantly exceeded the WHO safety limit (0.6 µg/L) for arsenic, with a measured mean value of 0.96 µg/L. Moreover, the levels of chromium (mean value: 2.63 µg/L) in 34 % of breast milk samples exceed the WHO recommended safety level (chromium: 1.5 µg/L). Aquatic foods accounted for 60 % to 90 % of the total intake of arsenic, cadmium, vanadium, mercury, and lead. The Spearman correlation analysis demonstrated strong positive correlations among breast milk metal elements, including copper-zinc (r = 0.68) and nickel­chromium (r = 0.89). Furthermore, the food-to-milk accumulation factors (FMAF) of strontium, nickel, arsenic, vanadium, cadmium, and mercury were relatively low (median values <0.005). While the FMAF values for chromium and lead were higher, with median values of 0.038 and 0.07, respectively. The results indicated potential risks of the toxic metal arsenic in breast milk from Dalian, China for breastfed infants. Therefore, continuous monitoring of breast milk for toxic metals and foodborne contamination is necessary.


Assuntos
Intoxicação por Arsênico , Arsênio , Mercúrio , Metais Pesados , Oligoelementos , Lactente , Feminino , Humanos , Cádmio/análise , Arsênio/análise , Leite Humano/química , Níquel/análise , Oligoelementos/análise , Vanádio/análise , Mercúrio/análise , Cromo/análise , Intoxicação por Metais Pesados , Ingestão de Alimentos , Metais Pesados/análise
5.
Sci Total Environ ; 905: 167187, 2023 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-37748602

RESUMO

The microbial fuel cell (MFC) is a promising bio-electrochemical technology that enables simultaneous electricity generation and effluent purification. Harnessing solar energy to provide sustainable power for MFC operation holds great potential. In this study, a semiartificial photosynthetic self-circulating MFC ecosystem is successfully established through the collaboration of electrogenic microorganisms and photosynthetic algae. The ecosystem can operate continuously without carbon sources and produces a voltage of 150 mV under irradiation. The irradiation doubles the maximum power density of the ecosystem, reaching 8.07 W/m2 compared to dark conditions. The results of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) suggest a higher diffusion capacity or faster electron replenishment ability within the ecosystem. Furthermore, the capacity of ecosystem for removing chromium (Cr(VI)) has been investigated comprehensively. Under irradiation, the ecosystem demonstrates a 2.25-fold increase in Cr(VI) removal rate compared to dark conditions. Finally, the results of 16S rRNA amplicon sequencing indicates an increase in the relative abundance of strict and facultative aerobic electroactive bacteria in the ecosystem, including Citrobacter (21 %), Bacillus (15 %) and Enterococcus (6 %). The ecosystem offers a novel, self-sustaining approach to address the challenges of energy recovery and environmental pollution.


Assuntos
Fontes de Energia Bioelétrica , Ecossistema , RNA Ribossômico 16S , Eletricidade , Fontes de Energia Bioelétrica/microbiologia , Bactérias/genética , Eletrodos
6.
Nano Lett ; 23(16): 7607-7614, 2023 08 23.
Artigo em Inglês | MEDLINE | ID: mdl-37527513

RESUMO

Accurate identification of tumor margins during cancer surgeries relies on a rapid detection technique that can perform high-throughput detection of multiple suspected tumor lesions at the same time. Unfortunately, the conventional histopathological analysis of frozen tissue sections, which is considered the gold standard, often demonstrates considerable variability, especially in many regions without adequate access to trained pathologists. Therefore, there is a clinical need for a multitumor-suitable complementary tool that can accurately and high-throughput assess tumor margins in every direction within the surgically resected tissue. We herein describe a high-throughput three-dimensional (3D) histological electrophoresis device that uses tumor-specific proteins to identify and contour tumor margins intraoperatively. Testing on seven cell-line xenograft models and human cervical cancer models (representing five types of tissues) demonstrated the high-throughput detection utility of this approach. We anticipate that the 3D histological electrophoresis device will improve the accuracy and efficiency of diagnosing a wide range of cancers.


Assuntos
Eletroforese , Margens de Excisão , Neoplasias , Humanos , Neoplasias/diagnóstico , Animais
7.
Sci Adv ; 9(26): eadg2690, 2023 06 30.
Artigo em Inglês | MEDLINE | ID: mdl-37390200

RESUMO

Tissue diagnosis is important during surgical excision of solid tumors for margin evaluation. Conventional histopathologic methods rely heavily on image-based visual diagnosis by specialized pathologists, which can be time-consuming and subjective. We report a three-dimensional (3D) histological electrophoresis system for rapid labeling and separation of the proteins within tissue sections, providing a more precise assessment of tumor-positive margin in surgically resected tissues. The 3D histological electrophoresis system uses a tumor-seeking dye labeling strategy to visualize the distribution of tumor-specific proteins within sections and a tumor finder that automatically predicts the tumor contour. We successfully demonstrated the system's capability to predict the tumor contours from five murine xenograft models and distinguish the tumor-invaded region of sentinel lymph nodes. Specifically, we used the system to accurately assess tumor-positive margins from 14 patients with cancer. Our 3D histological electrophoresis system serves as an intraoperative tissue assessment technology for more accurate and automatic pathologic diagnosis.


Assuntos
Proteínas de Neoplasias , Tecnologia , Humanos , Animais , Camundongos , Metástase Linfática , Modelos Animais de Doenças , Eletroforese
8.
Environ Sci Pollut Res Int ; 30(35): 84141-84151, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37355514

RESUMO

Microbial-mineral interaction has a broad application prospect in the field of environmental remediation of organic pollutants. However, the disadvantages of long repair cycle and low repair rate limit its industrial application. In this study, natural hematite was used as an auxiliary material for soil remediation in a bio-electrochemical system. It was found that the power density of soil microbial fuel cell (SMFC) system composed of 2.0 mm hematite was 2.889 mW/m2, which is 2.7 times compared with the blank group (1.068 mW/m2) in the particle size optimization experiment. A similarly increased power density (1.068 to 2.467 mW/m2) was observed when the hematite content changed from 0 to 20% in the concentration optimization experiment. Under 20% and 2.0-mm hematite condition, the phenol removal rate was closed to 99% after 7 days, which is 1.9-folds compared with blank control (53%). These results suggest that addition of hematite enhances soil porosity and conductivity, and increases the number of electron acceptors in soil. These findings inspire that this economic and abundant natural mineral is expected to be a potential auxiliary material in the field of soil organic pollutant purification, and expand the understanding of interactions between hematite and microorganisms in nature.


Assuntos
Fontes de Energia Bioelétrica , Poluentes Ambientais , Recuperação e Remediação Ambiental , Poluentes do Solo , Solo/química , Compostos Férricos , Poluentes do Solo/análise , Minerais
9.
Front Plant Sci ; 14: 1140727, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36895872

RESUMO

DNA-binding with one finger (Dof) transcription factors play a crucial role in plant abiotic stress regulatory networks, although massive Dofs have been systematically characterized in plants, they have not been identified in the hexaploid crop sweetpotato. Herein, 43 IbDof genes were detected to be disproportionally dispersed across 14 of the 15 chromosomes of sweetpotato, and segmental duplications were discovered to be the major driving force for the expansion of IbDofs. The collinearity analysis of IbDofs with their related orthologs from eight plants revealed the potential evolutionary history of Dof gene family. Phylogenetic analysis displayed that IbDof proteins were assigned into nine subfamilies, and the regularity of gene structures and conserved motifs was consistent with the subgroup classification. Additionally, five chosen IbDof genes were shown to be substantially and variably induced under various abiotic conditions (salt, drought, heat, and cold), as well as hormone treatments (ABA and SA), according to their transcriptome data and qRT-PCR experiments. Consistently, the promoters of IbDofs contained a number of cis-acting elements associated with hormone and stress responses. Besides, it was noted that IbDof2 had transactivation activity in yeasts, while IbDof-11/-16/-36 did not, and protein interaction network analysis and yeast two-hybrid experiments revealed a complicated interaction connection amongst IbDofs. Collectively, these data lay a foundation for further functional explorations of IbDof genes, especially with regards to the possible application of multiple IbDof members in breeding the tolerant plants.

10.
J Exp Med ; 220(4)2023 04 03.
Artigo em Inglês | MEDLINE | ID: mdl-36749798

RESUMO

Metastatic castration-resistant prostate cancer (PC) is the final stage of PC that acquires resistance to androgen deprivation therapies (ADT). Despite progresses in understanding of disease mechanisms, the specific contribution of the metastatic microenvironment to ADT resistance remains largely unknown. The current study identified that the macrophage is the major microenvironmental component of bone-metastatic PC in patients. Using a novel in vivo model, we demonstrated that macrophages were critical for enzalutamide resistance through induction of a wound-healing-like response of ECM-receptor gene expression. Mechanistically, macrophages drove resistance through cytokine activin A that induced fibronectin (FN1)-integrin alpha 5 (ITGA5)-tyrosine kinase Src (SRC) signaling cascade in PC cells. This novel mechanism was strongly supported by bioinformatics analysis of patient transcriptomics datasets. Furthermore, macrophage depletion or SRC inhibition using a novel specific inhibitor significantly inhibited resistant growth. Together, our findings elucidated a novel mechanism of macrophage-induced anti-androgen resistance of metastatic PC and a promising therapeutic approach to treat this deadly disease.


Assuntos
Neoplasias Ósseas , Neoplasias de Próstata Resistentes à Castração , Masculino , Humanos , Antagonistas de Androgênios/uso terapêutico , Neoplasias de Próstata Resistentes à Castração/tratamento farmacológico , Neoplasias de Próstata Resistentes à Castração/metabolismo , Neoplasias de Próstata Resistentes à Castração/patologia , Linhagem Celular Tumoral , Macrófagos/metabolismo , Receptores Androgênicos/genética , Nitrilas/uso terapêutico , Microambiente Tumoral
12.
EMBO J ; 42(4): e111549, 2023 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-36598329

RESUMO

YAP/TAZ transcriptional co-activators play pivotal roles in tumorigenesis. In the Hippo pathway, diverse signals activate the MST-LATS kinase cascade that leads to YAP/TAZ phosphorylation, and subsequent ubiquitination and proteasomal degradation by SCFß-TrCP . When the MST-LATS kinase cascade is inactive, unphosphorylated or dephosphorylated YAP/TAZ translocate into the nucleus to mediate TEAD-dependent gene transcription. Hippo signaling-independent YAP/TAZ activation in human malignancies has also been observed, yet the mechanism remains largely elusive. Here, we report that the ubiquitin E3 ligase HERC3 can promote YAP/TAZ activation independently of its enzymatic activity. HERC3 directly binds to ß-TrCP, blocks its interaction with YAP/TAZ, and thus prevents YAP/TAZ ubiquitination and degradation. Expression levels of HERC3 correlate with YAP/TAZ protein levels and expression of YAP/TAZ target genes in breast tumor cells and tissues. Accordingly, knockdown of HERC3 expression ameliorates tumorigenesis of breast cancer cells. Our results establish HERC3 as a critical regulator of the YAP/TAZ stability and a potential therapeutic target for breast cancer.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Neoplasias da Mama , Humanos , Feminino , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Transativadores/genética , Transativadores/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/genética , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Proteínas de Sinalização YAP , Proteínas Contendo Repetições de beta-Transducina/genética , Proteínas Contendo Repetições de beta-Transducina/metabolismo , Proteínas com Motivo de Ligação a PDZ com Coativador Transcricional , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Transformação Celular Neoplásica/genética , Carcinogênese/genética , Ubiquitinação , Neoplasias da Mama/genética , Ubiquitinas/metabolismo , Ligases/genética , Fosfoproteínas/genética , Fosfoproteínas/metabolismo
13.
Environ Sci Pollut Res Int ; 30(16): 46355-46367, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36717415

RESUMO

Rare earth elements (REEs) are considered environmental pollutants that have received extensive attention recently. The accumulation of REEs in plants is important because REEs can eventually enter the human body via the food chain. Marigolds are widely utilized as medicinal and commercial plants in medicine, feed, and therapeutics. Due to the extremely high demand for marigold in global, it is urgent to investigate the accumulation and distribution of REEs in marigold plants to reduce human and animal health risks. Marigold leaves tended to bioaccumulate the highest amounts of REEs from soil compared with other tissues. The distribution patterns of REEs in marigold were similar to those in the rhizosphere soil, which was enriched in light rare earth elements. Cerium accumulated most in marigold and soil, accounting for nearly 50% of ΣREEs, followed by lanthanum, neodymium, and yttrium. Roots were the most susceptible tissue affected by soil REE concentration, and a significant positive correlation was observed for REEs in the roots of marigold and soils (R = 0.87), while no significant correlation was observed for REEs in soils and other tissues. REEs were poorly transferred from soil to marigold, with bioaccumulation factor values for all tissues of marigold less than one. Additionally, REEs exhibited a positive correlation with Al and Fe in the roots, stems, leaves, and flowers of marigold. The present research revealed the biological interactions between marigold and soil and the distribution of REEs in various parts of marigold. It provides a reference for large-scale commercial cultivation and potential environmental risk in the future.


Assuntos
Calendula , Cério , Metais Terras Raras , Poluentes do Solo , Tagetes , Animais , Humanos , Solo , Metais Terras Raras/análise , Lantânio , Plantas , Poluentes do Solo/análise
14.
Environ Sci Pollut Res Int ; 30(2): 4423-4434, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35969345

RESUMO

As a novel technique with a wide range of applications, microbial fuel cell (MFC) could simultaneously remove organic contaminants and heavy metals in complex wastewater, despite striking differences in physicochemical properties of these contaminant. But its wastewater treatment efficiency is restricted by its lower generation performance. However, approaches for the modification of MFCs' cathode with appropriate catalyst could effectively overcome this limitation. Herein, a new-type efficient cathode catalyst was invented through modifying natural molybdenite via one-step oxidation method. In this case, molybdenite had many changes in morphology (wave-shaped bending, fragmentation and decrescent diameter) during oxidation modification process, and oxidation-modified molybdenite could provide much more active sites for the cathode. After applying this novel cathode catalyst, the electric generation capacity of MFC system increased by 5.08 times, and its simultaneous degradation efficiency of methyl blue (MB) and Cr (VI) increased by 3.35 times (compared with graphite cathode MFC). This study provides a novel low-carbon and environmentally friendly way to prepare high efficiency cathode catalyst materials and provides a new idea of simultaneous purification for organic and metallic pollutants from complex wastewater.


Assuntos
Fontes de Energia Bioelétrica , Metais Pesados , Eletricidade , Águas Residuárias , Eletrodos
15.
Plant Physiol ; 191(1): 747-771, 2023 01 02.
Artigo em Inglês | MEDLINE | ID: mdl-36315103

RESUMO

Plants often simultaneously experience combined stresses rather than a single stress, causing more serious damage, but the underlying mechanisms remain unknown. Here, we identified the stress-induced IbNAC3 from sweet potato (Ipomoea batatas) as a nucleus-localized transcription activator. IbNAC3 contains a unique activation domain whose MKD sequence confers transactivation activities to multiple other TFs and is essential for the activated expression of downstream target genes. Ectopic expression of IbNAC3 conferred tolerance to single and combined salt and drought stresses in Arabidopsis (Arabidopsis thaliana), and a group of NAM, ATAF1/2, and CUC2 (NAC) TFs, including ANAC011, ANAC072, ANAC083, ANAC100, and NAP, interacted with IbNAC3, and the specific domains responsible for each interaction varied. Intriguingly, IbNAC3 repressed the interaction among the five NACs, and knockout or mutation of ANAC011 and ANAC072 dramatically impaired combined stress tolerance. IbNAC3-ANAC072 and IbNAC3-NAP modules synergistically activated the MICROTUBULE-RELATED E3 LIGASE57 (MREL57) gene. Consistently, mutation of MREL57 and overexpression of WAVE-DAM-PENED2-LIKE7, encoding a target protein of MREL57, both remarkably impaired combined stress tolerance. Moreover, transgenic plants displayed abscisic acid (ABA) hyposensitivity by directly promoting the transcription of ENHANCED RESPONSE TO ABA 1, a key negative regulator of ABA signaling. The data unravel the unique IbNAC3 TF functions as a pivotal component in combined stress tolerance by integrating multiple regulatory events and ubiquitin pathways, which is essential for developing high-tolerant plants in natural environments.


Assuntos
Arabidopsis , Ipomoea batatas , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Ipomoea batatas/genética , Ipomoea batatas/metabolismo , Secas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Estresse Fisiológico/genética , Regulação da Expressão Gênica de Plantas , Cloreto de Sódio/farmacologia , Ácido Abscísico/farmacologia , Ácido Abscísico/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Arabidopsis/metabolismo
16.
BMC Plant Biol ; 22(1): 616, 2022 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-36575404

RESUMO

BACKGROUND: WRKY transcription factors play pivotal roles in regulating plant multiple abiotic stress tolerance, however, a genome-wide systematical analysis of WRKY genes in sweetpotato is still missing. RESULTS: Herein, 84 putative IbWRKYs with WRKY element sequence variants were identified in sweetpotato reference genomes. Fragment duplications, rather than tandem duplications, were shown to play prominent roles in IbWRKY gene expansion. The collinearity analysis between IbWRKYs and the related orthologs from other plants further depicted evolutionary insights into IbWRKYs. Phylogenetic relationships displayed that IbWRKYs were divided into three main groups (I, II and III), with the support of the characteristics of exon-intron structures and conserved protein motifs. The IbWRKY genes, mainly from the group Ib, displayed remarkable and diverse expression profiles under multiple abiotic stress (NaCl, PEG6000, cold and heat) and hormone (ABA, ACC, JA and SA) treatments, which were determined by RNA-seq and qRT-PCR assays, suggesting their potential roles in mediating particular stress responses. Moreover, IbWRKY58L could interact with IbWRKY82 as revealed by yeast two-hybrid based on the protein interaction network screening. And abiotic stress-remarkably induced IbWRKY21L and IbWRKY51 were shown to be localized in the nucleus and had no transactivation activities. CONCLUSION: These results provide valuable insights into sweetpotato IbWRKYs and will lay a foundation for further exploring functions and possible regulatory mechanisms of IbWRKYs in abiotic stress tolerance.


Assuntos
Ipomoea batatas , Fatores de Transcrição , Fatores de Transcrição/metabolismo , Genoma de Planta , Filogenia , Ipomoea batatas/genética , Ipomoea batatas/metabolismo , Estresse Fisiológico/genética , Família Multigênica , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/metabolismo
17.
Materials (Basel) ; 15(21)2022 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-36363044

RESUMO

At present, many experimental fast reactors have adopted alloy nuclear fuels, for example, U-Zr alloy fuels. During the neutron irradiation process, vacancies and hydrogen (H) impurity atoms can both exist in U-Zr alloy fuels. Here, first-principles density functional theory (DFT) is employed to study the behaviors of vacancies and H atoms in disordered-γ(U,Zr) as well as their impacts on the electronic structure and mechanical properties. The formation energy of vacancies and hydrogen solution energy are calculated. The effect of vacancies on the migration barrier of hydrogen atoms is revealed. The effect of vacancies and hydrogen atom on densities of states and elastic constants are also presented. The results illustrate that U vacancy is easier to be formed than Zr vacancy. The H interstitial prefers the tetrahedral site. Besides, U vacancy shows H-trap ability and can raise the H migration barrier. Almost all the defects lead to decreases in electrical conductivity and bulk modulus. It is also found that the main effect of defects is on the U-5f orbitals. This work provides a theoretical understanding of the effect of defects on the electronic and mechanical properties of U-Zr alloys, which is an essential step toward tailoring their performance.

18.
Drug Des Devel Ther ; 16: 1931-1945, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35762015

RESUMO

Purpose: Anoectochilus roxburghii (Wall.) Lindl. polysaccharides (ARPs) have been reported to exhibit multiple pharmacological activities including anti-inflammatory and anti-hyperglycemia. This study aims to investigate the effect of ARPs on cognitive dysfunction induced by high fat diet (HFD). Methods: Six-week-old male mice were treated with ARPs by dietary supplementation for 14 weeks. The effect of ARPs on cognitive function was determined by assessing the changes in spatial learning and memory ability, neurotrophic factors in hippocampus, inflammatory parameters, intestinal barrier integrity, and gut microbiota. Results: ARPs supplementation can effectively ameliorate cognitive dysfunction, decrease the phosphorylation levels of Tau protein in hippocampus. Meanwhile, the increased body weight, plasma glucose, total cholesterol, inflammatory factors induced by HFD were abolished by ARPs treatment. Furthermore, ARPs treatment restored the intestinal epithelial barrier as evidenced by upregulation of intestinal tight junction proteins. Additionally, ARPs supplementation significantly decreased the relative abundance of several bacteria genus such as Parabacteroides, which may play regulatory roles in cognitive function. Conclusion: These results suggest that ARPs might be a promising strategy for the treatment of cognitive dysfunction induced by HFD. Mechanistically, alleviation of cognitive dysfunction by ARPs might be associated with the "gut-brain" axis.


Assuntos
Disfunção Cognitiva , Orchidaceae , Animais , Encéfalo , Disfunção Cognitiva/tratamento farmacológico , Dieta Hiperlipídica , Suplementos Nutricionais , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Polissacarídeos/farmacologia , Polissacarídeos/uso terapêutico
19.
BMC Plant Biol ; 22(1): 232, 2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35524176

RESUMO

BACKGROUND: The plant-specific GRAS transcription factors play pivotal roles in various adverse environmental conditions. Numerous GRAS genes have been explored and characterized in different plants, however, comprehensive survey on GRASs in sweetpotato is lagging. RESULTS: In this study, 72 putative sweetpotato IbGRAS genes with uneven distribution were isolated on 15 chromosomes and classified into 12 subfamilies supported by gene structures and motif compositions. Moreover, both tandem duplication and segmental duplication events played critical roles in the expansion of sweetpotato GRAS genes, and the collinearity between IbGRAS genes and the related orthologs from nine other plants further depicted evolutionary insights into GRAS gene family. RNA-seq analysis under salt stress and qRT-PCR detection of 12 selected IbGRAS genes demonstrated their significant and varying inductions under multiple abiotic stresses (salt, drought, heat and cold) and hormone treatments (ABA, ACC and JA). Consistently, the promoter regions of IbGRAS genes harbored a series of stress- and hormone-associated cis-acting elements. Among them, IbGRAS71, the potential candidate for breeding tolerant plants, was characterized as having transactivation activity in yeasts, while IbGRAS-2/-4/-9 did not. Moreover, a complex interaction relationship between IbGRASs was observed through the interaction network analysis and yeast two-hybrid assays. CONCLUSIONS: Our results laid a foundation for further functional identifications of IbGRAS genes, and multiple members may serve as potential regulators for molecular breeding of tolerant sweetpotato.


Assuntos
Ipomoea batatas , Fatores de Transcrição , Regulação da Expressão Gênica de Plantas , Hormônios , Ipomoea batatas/genética , Ipomoea batatas/metabolismo , Família Multigênica , Filogenia , Melhoramento Vegetal , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Estresse Fisiológico/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
20.
Sci Total Environ ; 828: 154119, 2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35227721

RESUMO

The widespread use of rare earth elements (REEs) in agriculture and high-tech industry resulted in significant release of REEs into the environment. However, there is a scarcity of studies focusing on the presence of REEs in the food worldwide. The present study investigated the residual levels of REEs in 14 representative food categories collected from 33 major cities of China. The measured total REEs (ΣREE) levels in the foods of aquatic origin were 174.97 µg kg-1 wet weight (ww), which was 6.35 times higher than those of terrestrial origin. It is interesting to observe a trophic dilution effect for REEs in both terrestrial and aquatic food samples. REEs in food samples at low trophic levels exhibited relatively high REEs levels; while for high trophic level food, relatively low REEs levels were observed. The distribution patterns of REEs varied across the different food categories and regions, with Ce being the most abundant REEs in all food samples, followed by La, Nd and Sm. High levels of ΣREE in food samples were observed in Midland, while low levels were found in the Northeast. Cereals was the dominant contributor to the estimated daily intake of REEs. The health risk of REEs by daily food consumption in China was acceptable.


Assuntos
Metais Terras Raras , Agricultura , China , Dieta , Metais Terras Raras/análise , Medição de Risco
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