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1.
Arch Virol ; 168(9): 227, 2023 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-37566243

RESUMO

Feline coronavirus (FCoV) is the causative agent of feline infectious peritonitis and diarrhoea in kittens worldwide. In this study, a total of 73 feline diarrhoeal faecal samples were collected from animal hospitals and pet markets in ShanDong province from 2017 to 2019. FCoV was detected in 58.23% (46/73) of the samples, using the RT-PCR method. The results showed that the detection rate of FCoV in healthy cats and sick cats was 41.7% (10/24) and 81.6% (40/49), respectively. Full gene amplification and sequencing of the N, M, and S2 genes of FCoV isolates were performed. An amino acid mutation (M1058L) in the S2 gene was found that can be used as a marker for distinguishing feline enteric coronavirus (FECV) from feline infectious peritonitis virus (FIPV). This study provides new epidemiological information about FCoV that will aid in the prevention of FCoV in China.


Assuntos
Infecções por Coronavirus , Coronavirus Felino , Coronavirus Felino/genética , Infecções por Coronavirus/veterinária , Infecções por Coronavirus/virologia , Doenças do Gato/virologia , Animais , Gatos , Proteínas do Nucleocapsídeo de Coronavírus/genética , Proteínas M de Coronavírus/genética , Glicoproteína da Espícula de Coronavírus/genética , Masculino , Feminino
2.
J Med Virol ; 92(10): 1971-1974, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32320083

RESUMO

The coronavirus disease 2019 pandemic caused by the novel coronavirus SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) has claimed many lives worldwide. Wearing medical masks (MMs) or N95 masks ([N95Ms] namely N95 respirators) can slow the virus spread and reduce the infection risk. Reuse of these masks can minimize waste, protect the environment, and help solve the current imminent shortage of masks. Disinfection of used masks is needed for their reuse with safety, but improper decontamination can damage the blocking structure of masks. In this study, we demonstrated using the avian coronavirus of infectious bronchitis virus to mimic SARS-CoV-2 that MMs and N95Ms retained their blocking efficacy even after being steamed on boiling water for 2 hours. We also demonstrated that three brands of MMs blocked over 99% viruses in aerosols. The avian coronavirus was completely inactivated after being steamed for 5 minutes. Altogether, this study suggested that MMs are adequate for use on most social occasions and both MMs and N95Ms can be reused for a few days with steam decontamination between use.


Assuntos
COVID-19/prevenção & controle , Desinfecção/métodos , Reutilização de Equipamento , Máscaras/virologia , Respiradores N95/virologia , Vapor , Gammacoronavirus , Humanos , Pandemias , SARS-CoV-2
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