RESUMO
Two novel cyclopeptides with special skeleton, namely, dolyemycins A (1) and B (2) were isolated from Streptomyces griseus subsp. griseus HYS31 by bio-guided isolation. Their structures were elucidated by detailed analysis of spectroscopic data. These two compounds were cyclopeptides containing eleven amino acids including five unusual amino acids (hydroxyglycine, 3-hydroxyleucine, 3-phenylserine, ß-hydroxy-O-methyltyrosine, 2,3-diaminobutyric acid) in both of them and an extra nonprotein amino acids (3-methylaspartic acid) in Dolyemycin B only. Dolyemycins A and B performed antiproliferative activity against human lung cancer A549 cells with IC50 values of 1.0 and 1.2 µM, respectively.
Assuntos
Peptídeos Cíclicos/química , Streptomyces griseus/química , Células A549 , Sequência de Aminoácidos , Antineoplásicos/química , Antineoplásicos/metabolismo , Antineoplásicos/farmacologia , Humanos , Modelos Moleculares , Peptídeos Cíclicos/metabolismo , Peptídeos Cíclicos/farmacologia , Conformação Proteica , Streptomyces griseus/metabolismoRESUMO
Two novel γ-butyrolactones ghanamycins A (1) and B (2) were isolated from the fermentation broth of marine-derived Streptomyces ghanaensis TXC6-16. Their structures were elucidated by spectroscopic analysis. These two novel compounds exhibited antimicrobial activities against some phytopathogens. The minimum IC (MIC) of 2 against Pseudomonas syringae and Erwinia sp. were 50 µg ml-1.
Assuntos
4-Butirolactona/análogos & derivados , 4-Butirolactona/isolamento & purificação , Anti-Infecciosos/isolamento & purificação , Streptomyces/metabolismo , 4-Butirolactona/química , 4-Butirolactona/farmacologia , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Erwinia/efeitos dos fármacos , Fermentação , Testes de Sensibilidade Microbiana , Pseudomonas syringae/efeitos dos fármacos , Análise EspectralRESUMO
Two new cyclic lipopeptides maribasins A (1) and B (2) were isolated from the fermentation broth of the marine microorganism Bacillus marinus B-9987 isolated from Suaeda salsa in Bohai coastline of P. R. China. Both structures were established to be cyclo (D-Pro-L-Gln-L-Asn-L-Ser-D-Asn¹-D-Tyr-D-Asn²-D-ß-aminoisopentadecanoic acid) (1) and cyclo (D-Pro-L-Gln-L-Asn-L-Ser-D-Asn¹-D-Tyr-D-Asn²-D-ß-aminoanteisopentadecanoic acid) (2) by spectroscopic analysis and exhibited broad-spectrum activity against phytopathogens by the antifungal bioassay.
Assuntos
Antifúngicos/química , Bacillus/metabolismo , Lipopeptídeos/química , Peptídeos Cíclicos/química , Sequência de Aminoácidos , Antifúngicos/isolamento & purificação , Antifúngicos/farmacologia , Lipopeptídeos/isolamento & purificação , Lipopeptídeos/farmacologia , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Conformação Molecular , Peptídeos Cíclicos/isolamento & purificação , Peptídeos Cíclicos/farmacologiaRESUMO
The new triterpenoid taraxerol-3-beta-O-tridecyl ether was isolated from the aerial part of Derris triofoliata. The structure was established on the basis of spectral data.