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Quartz tuning fork (QTF)-based techniques of photoacoustic spectroscopy and thermoelastic spectroscopy play a significant role in trace gas sensing due to unique high sensitivity and compactness. However, the stability of both techniques remains plagued by the inevitable and unpredictable laser power variation and demodulation phase variation. Herein, we investigate the phase change of a QTF when integrating both techniques for enhanced gas sensing. By demonstrating harmonic phase-sensitive methane detection as an example, we achieve stable gas measurement at varying laser power (2.4-9.4â¯mW) and varying demodulation phase (-90-90°). Besides, this method shows more tolerance to resonant frequency drift, contributing to a small signal fluctuation of ≤ 6.4â¯% over a wide modulation range (>10 times of the QTF bandwidth). The realization of harmonic-phase detection allows strengthening the stability of QTF-based sensors in a simple manner, especially when stable parameters, such as laser power, demodulation phase, even resonant frequency, cannot always be maintained.
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Stormwater management problems have been deteriorated with the high frequency and intensity of precipitation owing to rapid urbanization and climate variability, especially in urban highly built-up area. Nature-based solutions (NBS) have emerged as a powerful measure for rising these challenges due to their ability to mitigate urban waterlogging and increase carbon sequestration. Identifying optimal locations for NBS implementation remains a critical research focus. This study integrates carbon and water footprint accounting into NBS-based planning for sustainable urban development in central Guangzhou, China, to enhance urban flooding mitigation and carbon sequestration. Through carbon-water footprint analysis and weighting, we prioritized NBS planning. To achieve the objectives of increasing sequestered carbon and reducing urban runoff, three types of NBS-green roofs, permeable pavements, and bioretention systems-were optimized for a high-priority town street. The results showed that the implementation of green roof and permeable pavement reduced the surface runoff by 3.58 %, while the biological retention system reduced the runoff gray water by 27 %. Moreover, the application of green roof and biological retention increased the carbon sequestration by 2.57 million kg CO2-eq (life cycle). The findings of this study provide comprehensive insights into optimization of NBS planning based on carbon-water footprint accounting, facilitating to enhance mitigation of urban flooding vulnerability and carbon sequestration.
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BACKGROUND: Wilson disease (WD) is an autosomal recessive disorder caused by homozygous or compound heterozygous mutations in ATP7B. Clinical manifestations primarily involve liver and nervous system lesions, with rarely observed hematologic manifestations. CASE PRESENTATION: In the present case, a patient with WD presented with thrombocytopenia, giant platelets, and Döhle-like cytoplasmic inclusions in the leukocytes. Initially, the May-Hegglin anomaly was considered; however, whole-exome sequencing did not reveal any mutation in the MYH9 gene but a heterozygous mutation was found in (C.2804 C > T, p.T935M) in the ATP7B gene. After two years, the patient developed tremors in his hands, lower limb stiffness, and foreign body sensation in the eyes. Additionally, Kayser-Fleischer rings in the corneal limbus were detected by slit-lamp examination. Copper metabolism test indicated a slight decrease in serum ceruloplasmin. Transmission electron microscopy revealed that the inclusion bodies of leukocytes were swollen mitochondria. Mass spectrometry analysis showed that the copper levels were almost 20-fold higher in the leukocytes of the patient than in those of the control group. Based on the Leipzig scoring system, a diagnosis of WD was confirmed. Zinc sulfate treatment ameliorated the patient's symptoms and enhanced platelet, serum ceruloplasmin, and albumin levels. CONCLUSIONS: In conclusion, this case represents the first documented instance of WD presenting as thrombocytopenia, giant platelets, and Döhle-like cytoplasmic inclusions in the leukocytes. Excessive cellular copper accumulation likely underlies these findings; however, understanding precise mechanisms warrants further investigation.
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Degeneração Hepatolenticular , Corpos de Inclusão , Leucócitos , Trombocitopenia , Humanos , ATPases Transportadoras de Cobre/genética , ATPases Transportadoras de Cobre/metabolismo , Degeneração Hepatolenticular/patologia , Degeneração Hepatolenticular/genética , Degeneração Hepatolenticular/complicações , Corpos de Inclusão/patologia , Corpos de Inclusão/metabolismo , Leucócitos/patologia , Leucócitos/metabolismo , Mutação , Trombocitopenia/patologiaRESUMO
Flocculation is one of the most significant conditioning methods for sludge dewatering. In the study, a novel flocculant CS-TA, prepared through free radical-mediated conjugation of tannic acid (TA) and chitosan (CS), was proposed to improve sludge dewatering. The characterisation using Fourier transform infra-red spectroscopy and X-ray diffraction analysis shows that the CS chain was the backbone of CS-TA, and the presence of CS-TA aromatic rings confirmed the conjugation of CS with TA. Moreover, the conditioning of CS-TA yielded the best dewatering performance at 30â mg g TS-1 with the water content of sludge cake by press filtration (Wsc) of 59.78% ± 0.3% and capillary suction time (CST) of 11.8s ± 0.35â s, compared to 98.2% ± 0.15% and 56.2â s ± 0.16 in raw sludge. The results of different influencing factors (e.g. pH and temperature) on flocculation efficiency indicated that CS-TA possessed the capacity for enhancing sludge dewaterability over a wide range of pH, and the optimal temperature was observed to be 35â °C. Furthermore, the increase of particle size and zeta potential implied the addition of CS-TA favoured the formation of larger particles charge neutralisation and adsorption bridging effect. In addition, extracellular polymer substances (EPS) analysis indicated that the decrease in the polysaccharide and protein contents in EPS after CS-TA addition could increase the relative hydrophobicity of sludge. Moreover, the contents of heavy metals in sludge and their leaching toxicity and environmental risk were reduced. This study provides comprehensive insights into the exploration of CS-TA for sludge dewatering and the maintenance of ecological security in an eco-friendly.
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Percarbonate encompasses sodium percarbonate (SPC) and composite in-situ generated peroxymonocarbonate (PMC). SPC emerges as a promising alternative to hydrogen peroxide (H2O2), hailed for its superior transportation safety, stability, cost-effectiveness, and eco-friendliness, thereby becoming a staple in advanced oxidation processes for mitigating water pollution. Yet, scholarly literature scarcely explores the deployment of percarbonate-AOPs in eradicating organic contaminants from aquatic systems. Consequently, this review endeavors to demystify the formation mechanisms and challenges associated with reactive oxygen species (ROS) in percarbonate-AOPs, alongside highlighting directions for future inquiry and development. The genesis of ROS encompasses the in situ chemical oxidation of activated SPC (including iron-based activation, discharge plasma, ozone activation, photon activation, and metal-free materials activation) and composite in situ chemical oxidation via PMC (namely, H2O2/NaHCO3/Na2CO3, peroxymonosulfate/NaHCO3/Na2CO3 systems). Moreover, the ROS generated by percarbonate-AOPs, such as â¢OH, O2â¢-, CO3â¢-, HO2â¢-, 1O2, and HCO4-, can work individually or synergistically to disintegrate target pollutants. Concurrently, this review systematically addresses conceivable obstacles posing percarbonate-AOPs in real-world application from the angle of environmental conditions (pH, temperature, coexisting substances), and potential ecological toxicity. Considering the outlined challenges and advantages, we posit future research directions to amplify the applicability and efficacy of percarbonate-AOPs in tangible settings. It is anticipated that the insights provided in this review will catalyze the progression of percarbonate-AOPs in water purification endeavors and bridge the existing knowledge void.
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Carbonatos , Oxirredução , Águas Residuárias , Carbonatos/química , Águas Residuárias/química , Poluentes Químicos da Água/química , Purificação da Água/métodos , Peróxido de Hidrogênio/química , Espécies Reativas de OxigênioRESUMO
Various psychosocial and psychological interventions have been developed to reduce schizophrenia relapse prevention. A better understanding of these active interventions is important for clinical practice and for meaningful allocation of resources. However, no bibliometric analysis of this area has been conducted. Studies were retrieved from the Web of Science Core Collection database. The publication outputs and cooperation of institutions were visualized with Origin 2021. Global cooperation was visualized using ArcGIS Pro3.0. VOSviewer was used to generate visualizations of network of authors and keywords. The number of annual publications generally showed a fluctuating upward trend over the past 20 years. Germany published the most relevant articles (361, 26.76%). The Technical University of Munich was the most productive institution (70, 9.86%). Leucht Stefan published the most articles (46, 6.48%) and had the highest number of citations (4,375 citations). Schizophrenia Research published the most studies (39, 5.49%). Keywords were roughly classified into three clusters: cognitive behavioral therapy (CBT), family interventions and family psychoeducation and other factors related to interventions. The findings provided the current status of research on psychosocial and psychological interventions for schizophrenia relapse prevention from a bibliometric perspective. Recent research has mainly focused on CBT, family interventions and family psychoeducation.
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In acute promyelocytic leukemia (APL), hemorrhage, particularly intracranial hemorrhage, is the most common cause of early death. A central venous catheter (CVC) may provide a greater guarantee of safety and comfort to APL patients. However, CVCs have seldom been attempted in APL patients during induction therapy because of concerns about increasing the risk of hemorrhagic complications after this invasive procedure. To evaluate the hemorrhagic risk after CVC placement in APL patients during induction therapy, we retrospectively analyzed 95 newly diagnosed patients with APL from January 2010 to December 2022. Among these patients, 39 patients in the CVC group and 56 patients in the non-CVC group were included. Laboratory and clinical parameters of the two groups were collected and compared. There were no significant differences in median platelet, fibrinogen (Fbg), D-dimer, prothrombin time (PT), white blood count (WBC) and hemoglobin (Hb) between the CVC and non-CVC groups on the first day of the visit (day 0) and the following days (day 4, day 7, day 11, day 14, day 18 and day 21) (p = 0.382, p = 0.805, p = 0.456, p = 0.902, p = 0.901 and p = 0.097, respectively). The consumption of transfused platelets and Fbg was not significantly different between the CVC group and non-CVC group (5.0 vs. 4.5 units, p = 0.34, and 6.8 vs. 6.0, p = 0.36, respectively). The last day of platelet and Fbg transfusion was also not significantly different (21 vs. 19, p = 0.238 and 7.5 vs. 8.5, p = 0.684, respectively). The incidences of total hemorrhagic events and hemorrhagic death were lower in the CVC group than in the non-CVC group (17.9% vs. 37.5%, p = 0.04 and 0% vs. 16.1%, p = 0.01, respectively). The 30-day survival rate was not significantly different (92.3% vs. 82.1%, respectively, p = 0.145) for the CVC group compared with the non-CVC group. Our study suggested that CVCs did not increase the hemorrhagic risk in APL patients during induction therapy and that a CVC should be considered in this type of clinical situation.
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Rhinoviruses (RVs) are major causes of the common cold and are related to severe respiratory tract diseases, leading to a considerable economic burden and impacts on public health. Available and stable viral resources of rhinoviruses for laboratory use are important for promoting studies on rhinoviruses and further vaccine or therapeutic drug development. Reverse genetic technology can be useful to produce rhinoviruses and will help to promote studies on their pathogenesis and virulence. In this study, rhinovirus A89, an RV-A species that has been found to be highly involved in hospitalization triggered by RV infections, was selected to construct an infectious clone based on its sequence as a representative. The viral mRNA produced by a T7 RNA transcript system was transfected into H1-HeLa cells, and the rescued RV-A89 viruses were harvested and confirmed by sequencing. The rescued RV-A89 induced a similar cytopathic effect (CPE) and shared almost identical growth kinetics curves with parental RV-A89. Moreover, 9A7, a prescreened monoclonal antibody against the parental RV-A89, had a good and specific reaction with the rescued RV-A89, and further characterization showed almost the same morphology and protein composition of both viruses; thus, recombinant RV-A89 with similar biological characterization and virulence to the parental virus was obtained. In summary, the infectious clone of RV-A89 was successfully established, and the development of reverse genetic technology for rhinovirus will provide a framework for further studies on rhinoviruses.
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Swine wastewater (SW), characterized by highly complex organic and nutrient substances, poses serious impacts on aquatic environment and public health. Furthermore, SW harbors valuable resources that possess substantial economic potential. As such, SW treatment technologies place increased emphasis on resource recycling, while progressively advancing towards energy saving, sustainability, and circular economy principles. This review comprehensively encapsulates the state-of-the-art knowledge for treating SW, including conventional (i.e., constructed wetlands, air stripping and aerobic system) and resource-utilization-based (i.e., anaerobic digestion, membrane separation, anaerobic ammonium oxidation, microbial fuel cells, and microalgal-based system) technologies. Furthermore, this research also elaborates the key factors influencing the SW treatment performance, such as pH, temperature, dissolved oxygen, hydraulic retention time and organic loading rate. The potentials for reutilizing energy, biomass and digestate produced during the SW treatment processes are also summarized. Moreover, the obstacles associated with full-scale implementation, long-term treatment, energy-efficient design, and nutrient recovery of various resource-utilization-based SW treatment technologies are emphasized. In addition, future research prospective, such as prioritization of process optimization, in-depth exploration of microbial mechanisms, enhancement of energy conversion efficiency, and integration of diverse technologies, are highlighted to expand engineering applications and establish a sustainable SW treatment system.
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Fontes de Energia Bioelétrica , Águas Residuárias , Animais , Suínos , Estudos Prospectivos , Reatores Biológicos , TecnologiaRESUMO
INTRODUCTION: Epilepsy is a common neurological disorder that presents with challenging mechanisms and treatment strategies. This study investigated the neuroprotective effects of quinpirole on lithium chloride pilocarpine-induced epileptic rats and explored its potential mechanisms. METHODS: Lithium chloride pilocarpine was used to induce an epileptic model in rats, and the effects of quinpirole on seizure symptoms and cognitive function were evaluated. The Racine scoring method, electroencephalography, and Morris water maze test were used to assess seizure severity and learning and memory functions in rats in the epileptic group. Additionally, immunohistochemistry and Western blot techniques were used to analyze the protein expression levels and morphological changes in glutamate receptor 2 (GluR2; GRIA2), BAX, and BCL2 in the hippocampi of rats in the epileptic group. RESULTS: First, it was confirmed that the symptoms in rats in the epileptic group were consistent with features of epilepsy. Furthermore, these rats demonstrated decreased learning and memory function in the Morris water maze test. Additionally, gene and protein levels of GluR2 in the hippocampi of rats in the epileptic group were significantly reduced. Quinpirole treatment significantly delayed seizure onset and decreased the mortality rate after the induction of a seizure. Furthermore, electroencephalography showed a significant decrease in the frequency of the spike waves. In the Morris water maze test, rats from the quinpirole treatment group demonstrated a shorter latency period to reach the platform and an increased number of crossings through the target quadrant. Network pharmacology analysis revealed a close association between quinpirole and GluR2 as well as its involvement in the cAMP signaling pathway, cocaine addiction, and dopaminergic synapses. Furthermore, immunohistochemistry and Western blot analysis showed that quinpirole treatment resulted in a denser arrangement and a more regular morphology of the granule cells in the hippocampi of rats in the epileptic group. Additionally, quinpirole treatment decreased the protein expression of BAX and increased the protein expression of BCL2. CONCLUSION: The current study demonstrated that quinpirole exerted neuroprotective effects in the epileptic rat model induced by lithium chloride pilocarpine. Additionally, it was found that the treatment not only alleviated the rats' seizure symptoms, but also improved their learning and memory abilities. This improvement was linked to the modulation of protein expression levels of GLUR2, BAX, and BCL2. These findings provided clues that would be important for further investigation of the therapeutic potential of quinpirole and its underlying mechanisms for epilepsy treatment.
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Epilepsia , Fármacos Neuroprotetores , Ratos , Animais , Pilocarpina/toxicidade , Pilocarpina/uso terapêutico , Cloreto de Lítio/uso terapêutico , Fármacos Neuroprotetores/efeitos adversos , Quimpirol/efeitos adversos , Proteína X Associada a bcl-2/uso terapêutico , Epilepsia/induzido quimicamente , Epilepsia/tratamento farmacológico , Convulsões/induzido quimicamente , Convulsões/tratamento farmacológico , Modelos Animais de DoençasRESUMO
The operation of cavity-enhanced techniques usually requires independent pre-calibration of the mirror reflectivity to precisely quantify the absorption. Here we show how to directly calibrate the effective mirror reflectivity without using any gas samples of known concentration or high-speed optical/electrical devices. Leveraging a phase modulator to generate sidebands, we are able to record Pound-Drever-Hall error signals shaped by cavity modes that can reveal the effective reflectivity after waveform analysis. As an example, we demonstrated the reflectivity calibration of a pair of near-infrared mirrors over 80â nm with a free spectral range-limited resolution, illustrating a reflectivity uncertainty of 2 × 10-5 in the center part of the refection wavelength range of the mirrors and larger at the edges. With an effective reflectivity of 0.9982 (finesse â¼1746) inferred at 1531.6â nm, a short â¼ 8-cm Fabry-Pérot cavity achieved a minimum detectable absorption coefficient of 9.1 × 10-9â cm-1 for trace C2H2 detection. This method, by providing convenient calibration in an almost real-time manner, would enable more practical cavity-enhanced gas measurement even with potential mirror reflectivity degradation.
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Tetracycline is frequently found in various environments and poses significant ecological risks. Calcium hydroxide-modified biochar has shown potential as a material for removing multiple classes of pollutants from wastewater streams. The tetracycline-adsorption performance and mechanism of alkali-modified biochars derived from nine wastes (corn straw, rice straw, swine manure, cypress powder, wheat straw, peanut shell, walnut shell powder, soybean straw, and corncobs) were investigated in the study. Among the four alkalis tested, calcium hydroxide exhibited the most effective modification effects at a pyrolysis temperature of 500 °C. Straw biomass was most suitable to be modified by calcium hydroxide, and calcium hydroxide-modified biochar showed the highest adsorption performance for tetracycline. The maximum adsorption capacities were 8.22 mg g-1 for pristine corn straw biochar and 93.46 mg g-1 for calcium hydroxide-modified corn straw biochar. The tetracycline adsorption mechanism by calcium hydroxide-modified corn straw biochar involved hydrogen bonding, oxygen-containing functional groups, Ca2+ metal complexation, and electrostatic attraction. Consequently, calcium hydroxide-modified corn straw biochar emerges as an environment-friendly, cost-effective, and efficient tetracycline adsorbent.
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Commercialization applications of proton exchange membrane fuel cells (PEMFCs) are throttled by the durability issues of the electrodes prepared by using catalyst inks. Probing into a desirable catalyst/ionomer interface by adjusting the catalyst inks is an effective way for obtaining high-durability electrodes. The present study investigated quantitatively the catalyst/ionomer interfaces based on the viscosity (η) property of the isopropyl alcohol (IPA) and dipropylene glycol (DPG) nonaqueous mixture solvent for the first time. Accelerated stress test (AST) showed that η as one of the characteristic parameters of the solvent had a threshold effect on the durability of electrodes. The electrodes in the half-cell and single cell all exhibited the highest durability using IPA:DPG = 2:6 (η = 27.00 cP) as the dispersion solvent in this work, embodied by its ECSA loss rate, and the cell potential loss was minimum after AST. The ECSA loss mechanism showed that a fine catalyst/ionomer interface structure was created for the highest durability electrode by regulating the η values of the solvent, and the carbon corrosion loss (le) and Pt particle dissolution loss (ld) were weakened. Based on the molecular dynamics (MD) simulation and 19F NMR spectra results, the solvent ratio (various η and similar ε and δ) affected the dispersion states of the ionomer. For the catalyst inks with the highest durability (IPA:DPG = 2:6), the Nafion backbone and side chain presented a higher mobility behavior in the solvent and tended to show the structure of extension separation and the respective aggregation of hydrophilic/hydrophobic phases. Meanwhile, Pt slab models suggested that the side chain of Nafion more easily adhered to the Pt interface zone, while the backbone was pushed toward the carbon support interface zone as more DPG molecules distributed on the Pt surface, which reduced the dissolution of Pt particles and the corrosion of the carbon support. These catalyst/ionomer interface structures tailored by regulating the solvent η values provide insights into improving the electrode durability.
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Phosphite, an essential component in the biogeochemical phosphorus cycle, may make significant contributions to the bioavailable phosphorus pool as well as water eutrophication. However, to date, the potential impacts of coexisting photochemically active substances on the environmental fate and transformation of phosphite in aquatic environments have been sparsely elucidated. In the present study, the effect of zinc oxide nanoparticles (ZnO NPs), a widely distributed photocatalyst in aquatic environments, on phosphite phototransformation under simulated solar irradiation was systematically investigated. The physicochemical characteristics of the pristine and reacted ZnO NPs were thoroughly characterized. The results showed that the presence of ZnO NPs induced the indirect phototransformation of phosphite to phosphate, and the reaction rate increased with increasing ZnO NPs concentration. Through experiments with quenching and trapping free radicals, it was proved that photogenerated reactive oxygen species (ROS), such as hydroxyl radical (â¢OH), superoxide anion (O2â¢-), and singlet oxygen (1O2), made substantial contributions to phosphite phototransformation. In addition, the influencing factors such as initial phosphite concentration, pH, water matrixes (Cl-, F-, Br-, SO42-, NO3-, NO2-, HCO3-, humic acid (HA) and citric acid (CA)) were investigated. The component of generated precipitates after the phosphite phototransformation induced by ZnO NPs was still dominated by ZnO NPs, while the presence of amorphous Zn3(PO4)2 was identified. This work explored ZnO NPs-mediated phosphite phototransformation processes, indicating that nanophotocatalysts released into aquatic environments such as ZnO NPs may function as photosensitizers to play a beneficial role in the transformation of phosphite to phosphate, thereby potentially mitigating the toxicity of phosphite to aquatic organisms while exacerbating eutrophication. The findings of this study provide a novel insight into the comprehensive assessment of the environmental fate, potential ecological risk, and biogeochemical behaviors of phosphite in natural aquatic environments under the condition of combined pollution.
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The great challenges induced by global climate change coupled with rapid urbanization underline the growing urgency for a change in stormwater management with a novel integrated approach. This study conducted a comprehensive review on state-of-the-art knowledge in the research field of green storm infrastructure (GSI) using bibliometric analysis. A corpus of 3988 GSI-related publications (2000-2021) extracted from the Web of Science database was used to evaluate the scientific output in GSI research through the "Bibliometrix" R package and "CiteSpace". Ever since 2010, the number of publications per year exhibited an exponential increase, with the annual publication growth rate of 28.61%. Notably, the United States (23.55%) and China (19.58%) contributed most in GSI publications. "Water" (306) was identified as the most relevant journal in GIS research field, followed by "Sustainability" (252) and "Science of the Total Environment" (200). Cluster analysis unveiled the predominant research themes, i.e., "Conceptual development of GSI" (69.25%), "Adaptation of GSI" (46.89%), and "Performance evaluation of GSI practices" (18.28%). Research foci have generally shifted from conventional engineering-based frameworks (e.g., reduce stormwater runoff and enhance water quality) to ecological-based multi-elements (e.g., preserve natural resources, augment urban biodiversity and optimize land-use patterns). This systematic review concludes trends, challenges and future research prospects of GSI, and aims to provide reference and guidance for decision-makers on the development of a more dynamic, resilient, and robust integrated GSI approach for sustainable urban stormwater management.
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Ecossistema , Qualidade da Água , Urbanização , Recursos Naturais , Biodiversidade , ChuvaRESUMO
Anti-NMDA receptor encephalitis is an autoimmune encephalitis well- known to pediatric neurologists. The characteristic combination of symptoms and detection of NMDA receptor antibody can confirm the diagnosis. Most children respond well to immunosuppressive therapy. Anti-GABAB receptor encephalitis usually occurs in adult patients. Most patients present clinically with symptoms of limbic encephalitis. Cases in pediatric patients are rare. Cerebral venous thrombosis also has a very low incidence in children without underlying diseases. Patients usually present with headaches, convulsions, and focal deficits. Anticoagulants are the first choice treatment. We report a boy initially diagnosed with Labbe vein thrombosis and later tested positive for both NMDA and GABAB receptors. Anticoagulants did not relieve the boy's symptoms, and immunosuppressive therapy achieved good results. The antibody titers were significantly reduced or even turned negative. Although the Labbe vein was not recanalized at four months follow-up, the brain lesion was significantly absorbed. We learn from this case that a child can be inflicted with cerebral venous thrombosis and autoimmune encephalitis simultaneously. Child patients respond well to treatment.
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Green infrastructure (GI) is used as an alternative and complement to traditional urban drainage system for mitigating urban stormwater issues mainly caused by climate change and urbanization. The combination of hydrological model and optimization algorithm can automatically find the optimal solution under multiple objectives. Given the multi-functional characteristics of GI, choosing the optimization objectives of GI are critical for multiple stakeholders. This study proposes a GI optimization method considering spatial functional zoning. Based on the basic conditions, the study area is divided into the flood risk control zone (FRCZ) and the total runoff control zone (TRCZ). The integrated model coupling hydrological model and optimization algorithm is applied to obtain the Pareto fronts and corresponding non-dominated solutions. The Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) method is used to support the decision-making process. The optimal solution obtained for the FRCZ achieves a flood risk reduction rate of 60.49% with an average life cycle cost per year of 0.20 × 108 Chinese Yuan (CNY); The optimal solution obtained for the TRCZ achieves a total runoff reduction rate of 22.83% with an average life cycle cost per year of 0.17 × 108 CNY. This study provides a reference for stakeholders in GI planning and design.
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Chuva , Urbanização , Hidrologia , Inundações , Algoritmos , CidadesRESUMO
Pneumonia is a condition characterized by lung damage resulting from a robust immune response by the host. While the defense and immunity against bacterial lung infections have been extensively studied, little is known about the specific immune factors involved in the progression of bacterial pneumonia. To address this knowledge gap, our study aimed to compare normal lung tissues with pneumonia tissues using various techniques, including HE staining, RNA sequencing, RT-PCR, and Elisa assay. Our analysis revealed a significant increase in the levels of interleukin-6 (IL-6) in pneumonia tissues compared to normal lung tissues. To further investigate the underlying mechanism, we extracted exosomes from both pneumonia and normal lung tissues using ultracentrifugation. The exosomes were then examined using electron microscopy, diameter analysis, and western blot assay. RNA sequencing of the exosomes revealed an upregulation of several microRNAs (miRNAs), with miR-362 exhibiting the most significant change. This finding was confirmed through RT-PCR analysis conducted on lung tissues and alveolar lavage fluid. To gain insights into the specific target genes of miR-362, we employed bioinformatics analysis, which identified VENTX as a potential target gene. This finding was further validated through RT-PCR, western blot, and luciferase assay. Our experimental evidence demonstrated that miR-362 regulates VENTX expression, as evidenced by the use of miR-362 mimics or inhibitors on lung cells. Furthermore, we discovered that exosomes derived from pneumonia tissues upregulate IL-6 production through the miR-362/VENTX axis. Importantly, the blocking of IL-6 generation, which is facilitated by miR-362 inhibitor and VENTX overexpression lentivirus, can be achieved by treating exosomes. Moreover, we conducted in vivo experiments using pneumonia models. Rats were treated with IL-6, miR-362 mimics, or VENTX knock-down lentivirus. The results demonstrated a worse prognosis for rats treated with these factors, indicating their potential as prognostic markers. Taken together, our study suggests that exosomes facilitate IL-6 generation by transferring miR-362, thereby suppressing VENTX transcription. Consequently, the IL-6/miR-362/VENTX axis emerges as a promising therapeutic target for pneumonia.
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Exossomos , MicroRNAs , Pneumonia , Ratos , Animais , Interleucina-6/genética , Interleucina-6/metabolismo , Exossomos/genética , MicroRNAs/genética , MicroRNAs/metabolismo , Pneumonia/genética , Pneumonia/metabolismo , Pulmão/metabolismoRESUMO
With the rapid economic development of China's coastal areas and the growth of industry and population, the problem of heavy metal contamination in estuarine waters is increasing in sensitivity and seriousness. In order to accurately and quantitatively describe the current status of heavy metal contamination and identify sensitive aquatic organisms with high ecological risks, five heavy metals in eight estuaries of the Pearl River were monitored at monthly intervals from January to December in 2020, and the ecological risks of aquatic organisms induced by heavy metals were evaluated using Risk quotients (RQ) and species sensitivity distributions (SSD) methods. The results showed that the concentrations of As, Cu, Pb, Hg and Zn in estuaries of the Pearl River were (0.65-9.25) µg/L, (0.07-11.57) µg/L, (0.05-9.09) µg/L, (< 0.40) µg/L and (0.67-86.12) µg/L, respectively. With the exception of Hg in Jiaomen water, the other heavy metals in each sampling site met or exceed the water quality standard of Grade II. The aquatic ecological risks of As, Pb and Hg were generally low in the waters of the Pearl River estuary, but individual aquatic organisms are subject to elevated ecological risks due to Cu and Zn. The content of Zn has a lethal effect on the crustaceans Temora Stylifera, and the content of Cu has a serious impact on the mollusks Corbicula Fluminea and has a certain impact on the crustaceans Corophium sp. and the fish Sparus aurata. Heavy metal levels and joint ecological risks (msPAF) in the Humen, Jiaomen, Hongqimen, and Hengmen estuaries were slightly higher than in other estuaries, and the Yamen estuary had the lowest contration of heavy metals and ecological risk. Research findings can serve as a basis for formulating water quality standards for heavy metals and for protecting aquatic biodiversity in the Pearl River Estuary.
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Copépodes , Mercúrio , Metais Pesados , Dourada , Poluentes Químicos da Água , Animais , Organismos Aquáticos , Monitoramento Ambiental , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/análise , Sedimentos Geológicos , Rios , Chumbo , Estuários , Metais Pesados/toxicidade , Metais Pesados/análise , Medição de Risco , ChinaRESUMO
Background: Anti-leucine-rich glioma-inactivated 1 (LGI1) encephalitis is infrequently reported but more and more recognizable in children. Here we give detailed description of the clinical features and long-term outcome of three cases of childhood onset anti-LGI1 encephalitis. Methods: Three anti-LGI1 encephalitis patients were hospitalized in the Department of Pediatrics at Qilu Hospital of Shandong University. Data about the clinical manifestations, treatments and long-term follow-up outcomes were described in detail. Results: Case 1 showed an adolescent girl with initiating symptom of acute-onset frequent focal seizures. Her serum LGI1-antibody test was positive, and she had a good response to antiseizure medication (ASM) and IVIG. Case 2 showed a preschool-age boy with long-period refractory focal seizures and recent behavioral change. Both serum and cerebrospinal fluid (CSF) tests of LGI1-antibody were positive, and the MRI showed progressive atrophy in the left hemisphere. The symptoms got improved after receiving second-line immunotherapy initially but there are still the sequelae of drug-resistant epilepsy and mild to moderate intellectual disability. Case 3 showed an adolescent boy with initiating symptom of acute-onset frequent focal seizures. Both serum and CSF tests of LGI1-antibody were positive, and he had a good response to immunotherapy. By analyzing all literature-reported 19 pediatric cases, we found pediatric anti-LGI1 encephalitis is more common in female and adolescent. Seizures and behavioral changes were the most common symptoms. CSF pleocytosis and LGI1-antibodies results were mostly negative. Most patients showed good response to immunotherapy. Conclusion: Childhood onset anti-LGI1 encephalitis is a heterogeneous clinical syndrome, ranging from typical limbic encephalitis to isolating focal seizures. It is important to test autoimmune antibodies when encountering similar cases and repeat antibody testing if necessary. Timely recognition leads to earlier diagnosis and more rapid initiation of effective immunotherapy and potentially better outcomes.