Molecularly imprinted electrochemical sensor based on metal-covalent organic framework for specifically recognizing norfloxacin from unpretreated milk.

Here, a molecularly imprinted electrochemical sensor (MIECS) was designed and fabricated for specifically monitoring norfloxacin (NFX), an entirely synthetic antibiotic. In which, Cu2+ dopped covalent organic framework (COF) was used to connect NFX imprinting layer and glassy carbon electrode through covalence. Under optimized conditions, the linear range is as wide as 5 orders of magnitude, and the detection limit is 5.94 × 10-3 µM (estimated based on S/N = 3). Average recoveries are among 92.4%-99.0% with relative standard deviations ≤ 4.05% (n = 3) in (spiked) whole, low-fat, and skimmed milk, validated by independent HPLC assays. The excellent performance can be ascribed to the significant recognition and enriching ability of the imprinting layer, improved conductivity of Cu2+ dopped covalent organic framework, and high stability of covalence between layers. We hope the work will act as a model of MIECSs for rapidly and selectively detecting trace drug residue in complex real samples.

Assessing genomic diversity and signatures of selection in Jiaxian Red cattle using whole-genome sequencing data.

BACKGROUND: Native cattle breeds are an important source of genetic variation because they might carry alleles that enable them to adapt to local environment and tough feeding conditions. Jiaxian Red, a Chinese native cattle breed, is reported to have originated from crossbreeding between taurine and indicine cattle; their history as a draft and meat animal dates back at least 30 years. Using whole-genome sequencing (WGS) data of 30 animals from the core breeding farm, we investigated the genetic diversity, population structure and genomic regions under selection of Jiaxian Red cattle. Furthermore, we used 131 published genomes of world-wide cattle to characterize the genomic variation of Jiaxian Red cattle. RESULTS: The population structure analysis revealed that Jiaxian Red cattle harboured the ancestry with East Asian taurine (0.493), Chinese indicine (0.379), European taurine (0.095) and Indian indicine (0.033). Three methods (nucleotide diversity, linkage disequilibrium decay and runs of homozygosity) implied the relatively high genomic diversity in Jiaxian Red cattle. We used θπ, CLR, FST and XP-EHH methods to look for the candidate signatures of positive selection in Jiaxian Red cattle. A total number of 171 (θπ and CLR) and 17 (FST and XP-EHH) shared genes were identified using different detection strategies. Functional annotation analysis revealed that these genes are potentially responsible for growth and feed efficiency (CCSER1), meat quality traits (ROCK2, PPP1R12A, CYB5R4, EYA3, PHACTR1), fertility (RFX4, SRD5A2) and immune system response (SLAMF1, CD84 and SLAMF6). CONCLUSION: We provide a comprehensive overview of sequence variations in Jiaxian Red cattle genomes. Selection signatures were detected in genomic regions that are possibly related to economically important traits in Jiaxian Red cattle. We observed a high level of genomic diversity and low inbreeding in Jiaxian Red cattle. These results provide a basis for further resource protection and breeding improvement of this breed.

Super-tough, anti-fatigue, self-healable, anti-fogging, and UV shielding hybrid hydrogel prepared via simultaneous dual in situ sol-gel technique and radical polymerization.

In this work, we propose a universal strategy to construct tough hybrid hydrogels simply by a dual in situ sol-gel reaction of vinyltriethoxysilane (VTES) and tetrabutyl titanate (TBOT), as well as an in situ radical polymerization of acrylamide (AM) and VTES. Interestingly, nano-SiO2 and nano-TiO2 acted as both multifunctional hybrid crosslinker and nanofiller in this hybrid hydrogel. Meanwhile, covalent bonding existed between TiO2 and SiO2, as well as polymers and SiO2, and non-covalent interactions existed between TiO2 and polymers, as well as the organic skeleton. The obtained hybrid hydrogel exhibited high tensile strength (38.78-330.50 kPa), medium tensile elastic modulus (26.53-120.48 kPa), ultrahigh compression strength (1.86-6.22 MPa), unprecedented fatigue resistance, and self-healability due to its unique hierarchical inorganic hybrid crosslinking mechanism. In addition, this hydrogel also displayed considerable anti-fogging and UV-shielding property. Hence, this hybrid hydrogel will have many potential uses in soft robots, substitutes for load-bearing tissues, and optical devices.