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A novel type of electron donor-acceptor system was built from a nitrogen-rich covalent organic framework (PC) and a polyoxometalate (BW12), fabricating a composite material (BW12@PC-250), which shows significantly improved photocatalytic H2O2 yield (56.4 µM h-1) under full spectrum illumination in pure water, being about 30 times higher than that of PC. This is due to the opening of the electron and proton transport pathway between PC and BW12, which paves a new way for POMs to modulate the photocatalytic reactions of COFs.
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BACKGROUND: Polygalacturonase (PG), a crucial enzyme involved in pectin degradation, is associated with various plants' developmental and physiological processes such as seed germination, fruit ripening, fruit softening and plant organ abscission. However, the members of PG gene family in sweetpotato (Ipomoea batatas) have not been extensively identified. RESULTS: In this study, there were 103 PG genes identified in sweetpotato genome, which were phylogenetically clustered into divergent six clades. The gene structure characteristics of each clade were basically conserved. Subsequently, we renamed these PGs according to their locations of the chromosomes. The investigation of collinearity between the PGs in sweetpotato and other four species, contained Arabidopsis thaliana, Solanum lycopersicum, Malus domestica and Ziziphus jujuba, revealed important clues about the potential evolution of the PG family in sweetpotato. Gene duplication analysis showed that IbPGs with collinearity relationships were all derived from segmental duplications, and these genes were under purifying selection. In addition, each promoter region of IbPG proteins contained cis-acting elements related to plant growth and development processes, environmental stress responses and hormone responses. Furthermore, the 103 IbPGs were differentially expressed in various tissues (leaf, stem, proximal end, distal end, root body, root stalk, initiative storage root and fibrous root) and under different abiotic stresses (salt, drought, cold, SA, MeJa and ABA treatment). IbPG038 and IbPG039 were down-regulated with salt, SA and MeJa treatment. According to the further investigation, we found that IbPG006, IbPG034 and IbPG099 had different patterns under the drought and salt stress in fibrous root of sweetpotato, which provided insights into functional differences among these genes. CONCLUSION: A total of 103 IbPGs were identified and classified into six clades from sweetpotato genome. The results of RNA-Seq and qRT-PCR suggested that IbPG006, IbPG034 and IbPG099 might play a significant role in tissue specificity as well as drought and salt stress responses, which showed valuable information for further functional characterization and application of the IbPGs.
Assuntos
Ipomoea batatas , Poligalacturonase , Poligalacturonase/genética , Ipomoea batatas/genética , Ipomoea batatas/metabolismo , Genoma de Planta/genética , Duplicação Gênica , Estresse Fisiológico , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Regulação da Expressão Gênica de Plantas , FilogeniaRESUMO
The xyloglucan endotransglucosylase/hydrolase (XET/XEH, also named XTH) family is a multigene family, the function of which plays a significant role in cell-wall rebuilding and stress tolerance in plants. However, the specific traits of the XTH gene family members and their expression pattern in different tissues and under stress have not been carried out in sweet potato. Thirty-six XTH genes were identified in I. batatas, all of which had conserved structures (Glyco_hydro_16). Based on Neighbor-Joining phylogenetic analysis the IbXTHs can be divided into three subfamilies-the I/II, IIIA, and IIIB subfamilies, which were unevenly distributed on 13 chromosomes, with the exception of Chr9 and Chr15. Multiple cis-acting regions related to growth and development, as well as stress responses, may be found in the IbXTH gene promoters. The segmental duplication occurrences greatly aided the evolution of IbXTHs. The results of a collinearity analysis showed that the XTH genes of sweet potato shared evolutionary history with three additional species, including A. thaliana, G. max, and O. sativa. Additionally, based on the transcriptome sequencing data, the results revealed that the IbXTHs have different expression patterns in leaves, stems, the root body (RB), the distal end (DE), the root stock (RS), the proximal end (PE), the initiative storage root (ISR), and the fibrous root (FR), and many of them are well expressed in the roots. Differentially expressed gene (DEG) analysis of FRs after hormone treatment of the roots indicated that IbXTH28 and IbXTH30 are up-regulated under salicylic acid (SA) treatment but down-regulated under methyl jasmonate (MeJA) treatment. Attentionally, there were only two genes showing down-regulation under the cold and drought treatment. Collectively, all of the findings suggested that genes from the XTH family are crucial for root specificity. This study could provide a theoretical basis for further research on the molecular function of sweet potato XTH genes.
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Ipomoea batatas , Ipomoea batatas/genética , Ipomoea batatas/metabolismo , Filogenia , Glicosiltransferases/metabolismo , Hidrolases/metabolismo , Regulação da Expressão Gênica de PlantasRESUMO
Heat treatment is a widely applied technique in the preservation of fruits and vegetables, effectively addressing issues such as disease management, rot prevention, and browning. In this study, we investigated the impact of heat treatment at 35 °C for 24 h on the quality characteristics and disease resistance of two sweet potato varieties, P32/P (Ipomoea batatas (L.) Lam. cv 'Pushu13') and Xinxiang (Ipomoea batatas (L.) Lam. cv 'Xinxiang'). The growth in vitro and reproduction of Rhizopus stolonifer were significantly inhibited at 35 °C. However, it resumed when returned to suitable growth conditions. The heat treatment (at 35 °C for 24 h) was found to mitigate nutrient loss during storage while enhancing the structural characteristics and free radical scavenging capacity of sweet potato. Additionally, it led to increased enzyme activities for APX, PPO, and POD, alongside decreased activities for Cx and PG, thereby enhancing the disease resistance of sweet potato against soft rot. As a result, the heat treatment provided a theoretical basis for the prevention of sweet potato soft rot and had guiding significance for improving the resistance against sweet potato soft rot.
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Photocatalytic H2O2 production is an innovative on-site H2O2 synthesis method to treat organic pollutants through Fenton-like reactions, avoiding the need and potential liability of H2O2 storage and transportation. Accurate quantification of H2O2 is crucial to explore the mechanism of photocatalytic H2O2 production and optimize reaction parameters. In this work, three common H2O2 quantification methods (i.e., titration with potassium permanganate (KMnO4), and colorimetry with ammonium metavanadate (NH4VO3) or N,N-diethylp-phenylenediamine-horseradish peroxidase (DPD-POD)) were compared and their susceptibility to interference by seven types of representative organics were considered. Interference mechanisms were explored based on the electron-donating (Egap) and electron-accepting (ELUMO) ability of the present organics. The accuracy of the KMnO4 titration method is greatly compromised by aromatic compounds even at 0.1 mM due to the increased KMnO4 consumption by direct oxidation. The presence of p-benzoquinone that directly reacts with NH4VO3 and DPD compromises these colorimetric methods, especially DPD-POD colorimetry at concentrations as low as 0.1 mM. The DPD-POD method should also be scrutinized in the presence of phenols due to significant disturbance by oxidation byproducts (e.g. hydroquinone inducing immediate color disappearance). A flowchart was generated to provide guidelines for selecting an appropriate H2O2 quantification method for different water matrices treated by Fenton-like reactions.
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Elétrons , Peróxido de Hidrogênio , Peroxidase do Rábano Silvestre/metabolismo , Oxirredução , Reprodutibilidade dos TestesRESUMO
Notopterol, isoimperatorin, volatile oil and extract ï¼water and ethanol) were used as the research objects in this study to investigate the effects of different softening method, slice thickness and drying methods on the quality of Notopterygii Rhizoma et Radix slices, and the experimental data were analyzed by homogeneous distance evaluation method. The results showed that different softening, cutting and drying processes could affect the content of five components in Notopterygii Rhizoma et Radix incisum. The best processing technology of Notopterygii Rhizoma et Radix slices was as follows: non-medicinal parts were removed; mildewed and rot as well as moth-eaten parts were removed; washed by the flowing drinking water; stacked in the drug pool; moistening method was used for softening, where 1/8 volume of water was sprayed for every 1 kg of herbs every 2 h; upper part of herbs covered with clean and moist cotton, and cut into thick slices (2-4 mm) after 12 h moistening until appropriate softness, then received blast drying for 4 h at 50 â, and turned over for 2 times during the drying. The process is practical and provides the experimental basis for the standardization of the processing of Notopterygii Rhizoma et Radix, with great significance to improve the quality of Notopterygii Rhizoma et Radix slices.
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Apiaceae/química , Medicamentos de Ervas Chinesas/normas , Extratos Vegetais/normas , Dessecação , Óleos Voláteis/química , Raízes de Plantas/química , Rizoma/químicaRESUMO
Study on the standardization of Chinese materia medica is an important action for modernization and globalization for traditional Chinese medicine. Standardization on the processing of Chinese herbal pieces is an important part in the study on standardization of Chinese materia medica, so it is of great significance to establish the technical processing standards of Angelicae Sinensis Radix pieces for improving its quality. In this study, single factor experiment was designed to optimize the softening, cutting and drying processes of Angelicae Sinensis Radix. With ferulic acid, Angelicae Sinensis Radix polysaccharide, volatile oil and extracts (water and ethanol) content as the quality index, the effects of different softening, cutting and drying processes on the contents of the five components in Angelicae Sinensis Radix were analyzed, and the normalized distance evaluation method was used to analyze the experimental data. The results showed that the content of five components in Angelicae Sinensis Radix was affected by different softening methods and drying temperature, but the thickness of slice had little effect on the content. The best preparation process for Angelicae Sinensis Radix was as follows: Non-medicinal parts were removed; mildewed and rot as well as moth-eaten parts were removed; washed by the flowing drinking water; stacked in the drug pool; moistening method was used for softening, where 125 mL water was sprayed for every 1 kg of herbs every 2.5 h; upper part of herbs covered with clean and moist cotton, and cut into thin slices (1-2 mm) after 15 h moistening until appropriate softness, with disk thickness of 1-2 cm, then received blast drying for 6 h at 55 â, and turned over for 2 times during the drying.
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Angelica sinensis/química , Medicamentos de Ervas Chinesas/normas , Extratos Vegetais/normas , Dessecação , Medicina Tradicional Chinesa , Óleos Voláteis/química , Raízes de Plantas/química , Polissacarídeos/química , Padrões de ReferênciaRESUMO
By the methods of adsorption and soil column leaching, a laboratory simulation test was conducted to study the effects of palygorskite application on the volatilization and leaching losses of urea nitrogen in loess soil. Comparing with applying urea fertilizer alone, the addition of palygorskite could decrease the volatilization rate of soil urea N at peak time, with the ammonia volatilization loss decreased by 13.6%-15.0%. When the palygorskite application rate was 0.3 and 0.6 g x kg(-1), the leaching rate of soil NH4(+) -N and NO3(-) -N decreased, with the leaching loss of soil mineral N decreased by 13.7% and 13.6%, respectively. Applying 0.9 g x kg(-1) of palygorskite application increased the leaching rate of soil NH4(+) -N and NO3(-) -N, with the leaching loss of soil mineral N increased by 6.1%. Applying 0.3 g x kg(-1) of palygorskite increased soil NH4(+) -N content by 0.20 mg x kg(-1), while applying 0.9 g x kg(-1) of palygorskite decreased soil NH4(+) -N content by 0.42 mg x kg(-1). Palygorskite application increased soil NO3(-) -N content by 1.24-2.52 mg x kg(-1). It was concluded that the application of palygorskite could decrease the volatilization rate of urea N, and applying appropriate amounts of palygorskite could decrease the leaching loss of soil mineral N and increase the contents of soil NH4(+) -N and NO3(-) -N.