A Multiple Mechanism Enhanced Arithmetic Optimization Algorithm for Numerical Problems.

The Arithmetic Optimization Algorithm (AOA) is a meta-heuristic algorithm inspired by mathematical operators, which may stagnate in the face of complex optimization issues. Therefore, the convergence and accuracy are reduced. In this paper, an AOA variant called ASFAOA is proposed by integrating a double-opposite learning mechanism, an adaptive spiral search strategy, an offset distribution estimation strategy, and a modified cosine acceleration function formula into the original AOA, aiming to improve the local exploitation and global exploration capability of the original AOA. In the proposed ASFAOA, a dual-opposite learning strategy is utilized to enhance population diversity by searching the problem space a lot better. The spiral search strategy of the tuna swarm optimization is introduced into the addition and subtraction strategy of AOA to enhance the AOA's ability to jump out of the local optimum. An offset distribution estimation strategy is employed to effectively utilize the dominant population information for guiding the correct individual evolution. In addition, an adaptive cosine acceleration function is proposed to perform a better balance between the exploitation and exploration capabilities of the AOA. To demonstrate the superiority of the proposed ASFAOA, two experiments are conducted using existing state-of-the-art algorithms. First, The CEC 2017 benchmark function was applied with the aim of evaluating the performance of ASFAOA on the test function through mean analysis, convergence analysis, stability analysis, Wilcoxon signed rank test, and Friedman's test. The proposed ASFAOA is then utilized to solve the wireless sensor coverage problem and its performance is illustrated by two sets of coverage problems with different dimensions. The results and discussion show that ASFAOA outperforms the original AOA and other comparison algorithms. Therefore, ASFAOA is considered as a useful technique for practical optimization problems.

Visible and infrared dual-band anti-counterfeiting with self-assembled photonic heterostructures.

Self-assembled photonic structures have greatly expanded the paradigm of optical materials due to their ease of access, the richness of results offered and the strong interaction with light. Among them, photonic heterostructure shows unprecedent advances in exploring novel optical responses that only can be realized by interfaces or multiple components. In this work, we realize visible and infrared dual-band anti-counterfeiting using metamaterial (MM) - photonic crystal (PhC) heterostructures for the first time. Sedimentation of TiO2 nanoparticles in horizontal mode and polystyrene (PS) microspheres in vertical mode self-assembles a van der Waals interface, connecting TiO2 MM to PS PhC. Difference of characteristic length scales between two components support photonic bandgap engineering in the visible band, and creates a concrete interface at mid-infrared to prevent interference. Consequently, the encoded TiO2 MM is hidden by structurally colored PS PhC and visualized either by adding refractive index matching liquid or by thermal imaging. The well-defined compatibility of optical modes and facility in interface treatments further paves the way for multifunctional photonic heterostructures.

Adeno-Associated Virus-Mediated Immunotherapy Based on Bispecific Tandem scFv for Alzheimer's Disease.

BACKGROUND: Patients with Alzheimer's disease (AD) have considerably increased globally as a result of population aging, placing a significant burden on the global economy and the medical system. The outcome of clinical trials for AD immunotherapy that solely targeted amyloid-ß (Aß) or phosphorylated tau protein (p-Tau) was unsatisfactory. Therefore, blocking both Aß and p-Tau's pathological processes simultaneously while also preventing their interaction may be the key to developing an effective AD therapy. OBJECTIVE: To develop a novel immunotherapy based on bispecific tandem scFv (TaFv) against AD. METHODS: Bispecific single-chain antibody that targets both Aß and p-Tau were obtained using E. coli expression system. Biological ability of TaFvs were determined by ELISA, SDS-PAGE, and immunohistochemical assay. Recombinant adeno-associated virus 9 (rAAV9) were packaged to create TaFv. The in vivo activity of rAAV9 were detected in mouse, using biophotonic imaging and frozen section methods. RESULTS: The outcomes demonstrated that both Aß and p-Tau had a high affinity for the bispecific TaFv. Additionally, it can bind to the amyloid plaques and neuronal tangles in the brain slices of an AD mouse model. Moreover, the rAAV9 could infect neuronal cells, transverse the blood-brain barrier, and express TaFv in the mouse brain. CONCLUSION: This novel immunotherapy offers a fresh concept for the immunotherapy of AD and successfully delivers the double target antibody into the brain, acting on both pathogenic substances Aß and p-Tau.

Nano-silica anti-icing coatings for protecting wind-power turbine fan blades.

Wind power is a promising electricity source. Nevertheless, wind turbine blade icing can cause severe problems in turbine operation. In this study, SiO2 spherical nanoparticles (∼90 nm), produced by RF (radio frequency) plasma spheroidization, were mixed with E51, PDMS, and ethyl acetate, and sprayed on the surface of aluminum plates and regular power windmill fan blades which were already coated with polyurethane primer. XPS and IR spectroscopies revealed the development of SiC and SiPh (Ph = phenolic ring) bonds, whose formation should be favored by the ultrasound and curing processes at 50 °C. The integrity of the coating/substrate interface, whose strength is ascribed to hydrogen bonds, was maintained after 100 icing-melting cycles. The coatings display superhydrophobic behavior and excellent anti-icing performance, along with stability in abrasion, sunlight and self-cleaning ability towards solid pollutants.

Lightweight MXene-Based Hybrid Aerogels with Ultrabroadband Terahertz Absorption and Anisotropic Strain Sensitivity.

MXene aerogels with a three-dimensional (3D) network structure have attracted increasing attention for lightweight electromagnetic wave absorbers. It is intriguing to expand their absorption band, i.e., to the booming terahertz (THz) region, and explore multifunctionality. Herein, we assemble MXene (Ti3C2Tx)-based hybrid aerogels into an aligned lamellar architecture using a bidirectional freezing technique. With air pore size and lamellar layer spacing comparable to THz wavelengths, high porosity of the aerogels allows nearly isotropic absorption of 99% and electromagnetic interference (EMI) shielding effectiveness with a remarkable value of 57.5 dB, in the ultrabroad bandwidth ranging from 0.5 to 3.0 THz. Simultaneous, strain-sensing response reflects the macroscopic anisotropy of the network structure of the aerogels. The improved sensitivity is measured for the out-of-lamellar layer plane under 0-30% strain. The corresponding long-term stability and durability persist over 120 stretching-releasing cycles. Our findings thus not only expand multiple functions of MXene in an anisotropic 3D macroscopic form but also clarify its nearly isotropic absorption in the THz band.

A critical review of Gross ecosystem product accounting in China: Status quo, problems and future directions.

As a measure of ecosystems' contribution to human well-being, the concept of Gross Ecosystem Product (GEP) is an integrated monetary index for the evaluation of final ecosystem services, which has attracted widespread attention around the world. In China, both national and local governments have launched a series of GEP accounting pilot projects, with the aim to incorporate this new concept into real world decision-making. However, a critical review of these practices remains lacking, especially regarding their current status and problems. In this study, by performing a systematic review and data integration of current literature and government documents, we comprehensively described the GEP accounting practices in China, including pilot project's coverage, accounting methods, and policy application. Then, we identified five major problems in current GEP accounting practices in China, which prevent GEP from being accurately measured in the short term. We proposed that GEP accounting should be a constantly evolving process with both long-term and short-term improvement goals. More in detail, the accuracy issues in GEP accounting require longer periods of time to resolve; while its repeatability, comparability, and applicability should be improved in the short term, so that it can be incorporated into decision-making. In response to these challenges, we suggested the adaptation of GEP accounting index screening principles as a possible future direction, which can help to apply GEP results in the current stages of decision making. By improving GEP concept and accounting, it will be possible to establish a unified comparable GEP accounting system and reduce the gap between the GEP and decision-making.

An adeno-associated virus-mediated immunotherapy for Alzheimer's disease.

Disease-modifying passive immunotherapies focusing on removal of abnormal phosphorylated Tau (p-Tau) constitute promising treatments for Alzheimer's disease (AD). Although several prior immunotherapies targeting p-Tau appear to be beneficial against AD, they have limitations such as the low blood-brain barrier (BBB) penetration rate, short half-life of antibodies, and the likelihood of inflammation. To address these issues, we designed a novel immunotherapy for AD. To this end, a single chain antibody (scFv) targeting p-Tau was generated, and a recombinant adeno-associated virus that can cross the BBB (rAAV/BBB) was used as a vector to express scFv for at least 22 weeks in the mouse brain. Results showed that the scFv constructed in this study had a high affinity to p-Tau and could bind to neuronal tangles in the section of brains of AD model mice. Moreover, the rAAV/BBB could cross the BBB, infect neuronal cells, and express scFv. This novel immunotherapy could effectively deliver scFv into the brain and resulted in a continuous expression of scFv in vivo, suggesting its potential for the treatment of AD.

Identification of a novel snoRNA expression signature associated with overall survival in patients with lung adenocarcinoma: A comprehensive analysis based on RNA sequencing dataset.

Since multiple studies have reported that small nucleolar RNAs (snoRNAs) can be serve as prognostic biomarkers for cancers, however, the prognostic values of snoRNAs in lung adenocarcinoma (LUAD) remain unclear. Therefore, the main work of this study is to identify the prognostic snoRNAs of LUAD and conduct a comprehensive analysis. The Cancer Genome Atlas LUAD cohort whole-genome RNA-sequencing dataset is included in this study, prognostic analysis and multiple bioinformatics approaches are used for comprehensive analysis and identification of prognostic snoRNAs. There were seven LUAD prognostic snoRNAs were screened in current study. We also constructed a novel expression signature containing five LUAD prognostic snoRNAs (snoU109, SNORA5A, SNORA70, SNORD104 and U3). Survival analysis of this expression signature reveals that LUAD patients with high risk score was significantly related to an unfavourable overall survival (adjusted P = 0.01, adjusted hazard ratio = 1.476, 95% confidence interval = 1.096-1.987). Functional analysis indicated that LUAD patients with different risk score phenotypes had significant differences in cell cycle, apoptosis, integrin, transforming growth factor beta, ErbB, nuclear factor kappa B, mitogen-activated protein kinase, phosphatidylinositol-3-kinase and toll like receptor signaling pathway. Immune microenvironment analysis also indicated that there were significant differences in immune microenvironment scores among LUAD patients with different risk score. In conclusion, this study identified an novel expression signature containing five LUAD prognostic snoRNAs, which may be serve as an independent prognostic indicator for LUAD patients.

An inverse averaging finite element method for solving three-dimensional Poisson-Nernst-Planck equations in nanopore system simulations.

The Poisson-Nernst-Planck (PNP) model plays an important role in simulating nanopore systems. In nanopore simulations, the large-size nanopore system and convection-domination Nernst-Planck (NP) equations will bring convergence difficulties and numerical instability problems. Therefore, we propose an improved finite element method (FEM) with an inverse averaging technique to solve the three-dimensional PNP model, named inverse averaging FEM (IAFEM). At first, the Slotboom variables are introduced aiming at transforming non-symmetric NP equations into self-adjoint second-order elliptic equations with exponentially behaved coefficients. Then, these exponential coefficients are approximated with their harmonic averages, which are calculated with an inverse averaging technique on every edge of each tetrahedral element in the grid. Our scheme shows good convergence when simulating single or porous nanopore systems. In addition, it is still stable when the NP equations are convection domination. Our method can also guarantee the conservation of computed currents well, which is the advantage that many stabilization schemes do not possess. Our numerical experiments on benchmark problems verify the accuracy and robustness of our scheme. The numerical results also show that the method performs better than the standard FEM when dealing with convection-domination problems. A successful simulation combined with realistic chemical experiments is also presented to illustrate that the IAFEM is still effective for three-dimensional interconnected nanopore systems.

Varied clinical significance of ATP-binding cassette C sub-family members for lung adenocarcinoma.

ABSTRACT: Lung adenocarcinoma (LUAD) is a lethal malignancy worldwide and a major public health concern. We explored the potential clinical significance for LUAD of ATP-binding cassette (ABC), sub-family C, consisting of ABCC1-6, 8-12, and cystic fibrosis transmembrane conductance regulator (CFTR).Five hundred LUAD patients from The Cancer Genome Atlas database were used for analysis, including differential expression and diagnostic and prognostic significance. Oncomine and MERAV databases were used to validate differential expression and diagnostic significance. A risk score model was constructed using prognosis-related ABCC members. Prognosis-related genes were further explored to correlate their expression with tumor stage progression. Interaction networks, including biological processes and metabolic pathways, were constructed using Cytoscape software and STRING website.ABCC1-3 consistently showed high expression in tumor tissues (all P ≤ 0.05). Most datasets indicated that ABCC5, 10, and 11 were highly expressed in tumor tissues whereas ABCC6, 9, and CFTR were highly expressed in nontumor tissues (all P ≤ 0.05). Diagnostic significance of ABCC3 and ABCC5 was consistently assessed and validated in three datasets (all area under the curve > 0.700) whereas ABCC6, 8, 10, 11, and CFTR were assessed in The Cancer Genome Atlas dataset and validated in one dataset (all area under the curve > 0.700). Prognostic analysis indicated that ABCC2, 6, and 8 mRNA expression was associated with survival of LUAD (all adjusted P ≤ .037). The risk score model constructed using ABCC2, 6, and 8 suggested prognostic significance for survival predictions. ABCC2 expression was associated with tumor stage, whereas ABCC6 and 8 were not. Interaction networks indicated that they were involved in establishment of localization, ion transport, plasma membrane, apical plasma membrane, adenylyl nucleotide binding, ABC transporters, ABC transporter disorders, ABC-family-protein-mediated transport, and bile secretion.Differentially expressed ABCC2 and ABCC5 might be diagnostic whereas ABCC2, 6, and 8 may be prognostic biomarkers for LUAD, possibly through ABC-family-mediated transporter disorders.

Experimental demonstration of an ultra-thin radar-infrared bi-stealth rasorber.

We propose a radar-infrared bi-stealth rasorber that not only provides broad microwave absorptivity and low infrared emissivity but also possesses a microwave transmission window at low frequency. It is composed of three functional layers, which are carefully designed to independently control the infrared emission, microwave absorption, and transmission, respectively. The structure exhibits broadband (8.1-19.3 GHz) and high-efficiency (>90%) absorption. A transmission window appears at low frequency with a transmission peak of 80% at 2.68 GHz. The thermal emissivity of the structure is about 0.27 in the atmosphere window, which is close to that of metal. Moreover, the total thickness of the proposed structure is only 3.713 mm. The low-infrared-emissivity, high-microwave-absorption and frequency-selective-transmission properties promise it will find potential applications in various stealth fields.

A sandwich temperature control membrane inlet mass spectrometer for dissolved gases and volatile organic compounds in aqueous solution.

A sandwich temperature control membrane inlet system based on a miniature mass spectrometer is presented that demonstrates improved analytical performance for the measurement of dissolved gases and volatile organic compounds (VOCs) in aqueous solution. Aqueous solution is directly brought to the monolayer flat membrane interface at a constant flow rate. A heating resistor and a thermocouple are fixed on the side of the membrane and aqueous solution respectively. This new strategy allows for a temperature compensation method, affording an improvement of sensitivity and a reduction of response time compared with the conventional heating solution temperature control strategy. Furthermore, a static heating mode is applied to effectively remove the memory effect. Automatic sampling and measurement are achieved by using the membrane inlet system with silicone sheeting of 50 µm thickness. The vacuum is below 3 × 10-5 Torr, which can make the instrument work normally. A good linear response is observed for benzene in the range of 0.1 ppm-10 ppm and the detection limit is 50 ppb. The analytical capacity of this system is demonstrated by the on-line analysis of VOCs in aqueous solution, in which the dominant ions are detected rapidly. The results indicate that the sandwich temperature control membrane inlet mass spectrometer (STC-MIMS) has a potential application for on-line analyzing organic pollution in aquatic environments.

Observation of nonreciprocal magnetophonon effect in nonencapsulated few-layered CrI3.

"Magneto-optical" effect refers to a rotation of polarization plane, which has been widely studied in traditional ferromagnetic metal and insulator films and scarcely in two-dimensional layered materials. Here, we uncover a new nonreciprocal magnetophonon Raman scattering effect in ferromagnetic few-layer CrI3 We observed a rotation of the polarization plane of inelastically scattered light between -20o and +60o that are tunable by an out-of-plane magnetic field from -2.5 to 2.5 T. It is experimentally observed that the degree of polarization can be magnetically manipulated between -20 and 85%. This work raises a new magneto-optical phenomenon and could create opportunities of applying two-dimensional ferromagnetic materials in Raman lasing, topological photonics, and magneto-optical modulator for information transport and storage.

Preparation and in vitro activity of single chain antibodies against Alzheimer's disease.

Disease-modifying passive immunotherapies focused on reducing amyloid-beta (Aß) deposition are a potential therapeutic strategy for Alzheimer's disease (AD). However, the results of Aß passive immunotherapy clinical trials were unsatisfactory, largely due to the low efficacy of whole antibodies due to their relatively large molecular weight and low blood-brain barrier transmittance. Furthermore, the constant region fragments of whole antibodies can trigger inflammatory reactions, which raises safety concerns. Single chain fragment variables (scFvs), containing only the variable region of the heavy and light chains of antibodies, show great potential for the treatment of AD. With the aim of generating a safe and effective AD passive immunotherapy, we designed and successfully prepared scFvs targeting Aß and investigated their activity in vitro. The results showed that both the 10D5-scFv and 12B4-scFv have high affinities for Aß monomers, oligomers, and fibers. Moreover, scFvs could prevent the formation of Aß oligomers and fibers, and block their cellular toxicity. In addition, 10D5-scFv and 12B4-scFv could bind to Aß plaques on the sections of mice brains in the in vitro study, indicating potential for the treatment of AD.

Ginsenoside Rg3 inhibits grass carp reovirus replication in grass carp ovarian epithelial cells.

Ginseng exhibits multiple medicinal properties, including the improvement of immune function and enhancing disease resistance. In this study, we investigated the inhibitory effects of ginsenoside Rg3 on grass carp reovirus (GCRV) infection of grass carp ovarian (CO) epithelial cells, in order to provide a baseline framework for future high-efficacy antiviral drug screening investigations. Ginsenoside Rg3 was added to GCRV-infected CO cells, and cells were cultured at 27 °C before cell proliferation was measured by MTT assays. Label-free real-time cellular analysis (RTCA) after 72 h of experimentation demonstrated that 100 µg/mL ginsenoside Rg3 treatment had the highest inhibitory effect on GCRV (among 1,10,100 µg/mL treatments). We then measured the capacity for cellular antioxidant ability. Cells treated with 1,10,100 µg/mL ginsenoside Rg3 exhibited increases in Total Antioxidant Capacity activity relative to controls, respectively. Furthermore, Antioxidant assay and reverse transcript quantitative polymerase chain reaction (RT-qPCR) showed that ginsenoside Rg3 were efficient to restrain the replication of GCRV in CO cells. Expression analysis of immune-related genes via RT-qPCR showed that treatment with ginsenoside Rg3 promoted expression of IRF-3 and IRF-7 increases, respectively. Moreover, expression of IFN-1 was induced, which then inhibition the expression of tumor necrosis factor-alpha (TNF-α). In conclusion, we demonstrated that ginsenoside Rg3 promotes CO cell proliferation, inhibits GCRV activity, promotes CO cell immune activities, and thereby enhances the resistance of CO to GCRV infection.

Enhanced Valley Zeeman Splitting in Fe-Doped Monolayer MoS2.

The "Zeeman effect" offers unique opportunities for magnetic manipulation of the spin degree of freedom (DOF). Recently, valley Zeeman splitting, referring to the lifting of valley degeneracy, has been demonstrated in two-dimensional transition metal dichalcogenides (TMDs) at liquid helium temperature. However, to realize the practical applications of valley pseudospins, the valley DOF must be controllable by a magnetic field at room temperature, which remains a significant challenge. Magnetic doping in TMDs can enhance the Zeeman splitting; however, to achieve this experimentally is not easy. Here, we report unambiguous magnetic manipulation of valley Zeeman splitting at 300 K (geff = -6.4) and 10 K (geff = -11) in a CVD-grown Fe-doped MoS2 monolayer; the effective Landé geff factor can be tuned to -20.7 by increasing the Fe dopant concentration, which represents an approximately 5-fold enhancement as compared to undoped MoS2. Our measurements and calculations reveal that the enhanced splitting and geff factors are due to the Heisenberg exchange interaction of the localized magnetic moments (Fe 3d electrons) with MoS2 through the d-orbital hybridization.

Novel candidate biomarkers of origin recognition complex 1, 5 and 6 for survival surveillance in patients with hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) has high morbidity and mortality and lacks effective biomarkers for early diagnosis and survival surveillance. Origin recognition complex (ORC), consisting of ORC1-6 isoforms, was examined to assess the potential significance of ORC isoforms for HCC prognosis. Methods: Oncomine and Gene Expression Profiling Interactive Analysis (GEPIA) databases were used to examine differential isoform expression, stage-specific expression, calculate Pearson correlations and perform survival analysis. A human protein atlas database was utilized to evaluate the protein expression of ORCs in liver tissue. The cBioPortal database was used to assess isoform mutations and the survival significance of ORCs in HCC. Cytoscape software was employed to construct gene ontologies, metabolic pathways and gene-gene interaction networks. Results: Differential expression analysis indicated that ORC1 and ORC3-6 were highly expressed in tumor tissues in the Oncomine and GEPIA databases, while ORC2 was not. All the ORCs were showed positive and statistically significant correlations with each other (all P<0.001). ORC1-2 and ORC4-6 expressions were associated with disease stages I-IV (all P<0.05), but ORC3 was not. Survival analysis found that ORC1 and ORC4-6 expressions were associated with overall survival (OS), and ORC1-3 and ORC5-6 expression were associated with recurrence-free survival (RFS; all P<0.05). In addition, low expression of these ORC genes consistently indicated better prognosis compared with high expression. Protein expression analysis revealed that ORC1 and ORC3-6 were expressed in normal liver tissues, whereas ORC2 was not. Enrichment analysis indicated that ORCs were associated with DNA metabolic process, sequence-specific DNA binding and were involved in DNA replication, cell cycle, E2F-enabled inhibition of pre-replication complex formation and G1/S transition. Conclusions: Differentially expressed ORC1, 5 and 6 are candidate biomarkers for survival prediction and recurrence surveillance in HCC.

Spin-Valley Locking Effect in Defect States of Monolayer MoS2.

Valley pseudospin in two-dimensional (2D) transition-metal dichalcogenides (TMDs) allows optical control of spin-valley polarization and intervalley quantum coherence. Defect states in TMDs give rise to new exciton features and theoretically exhibit spin-valley polarization; however, experimental achievement of this phenomenon remains challenges. Here, we report unambiguous valley pseudospin of defect-bound localized excitons in CVD-grown monolayer MoS2; enhanced valley Zeeman splitting with an effective g-factor of -6.2 is observed. Our results reveal that all five d-orbitals and the increased effective electron mass contribute to the band shift of defect states, demonstrating a new physics of the magnetic responses of defect-bound localized excitons, strikingly different from that of A excitons. Our work paves the way for the manipulation of the spin-valley degrees of freedom through defects toward valleytronic devices.

Oncogene PLCE1 may be a diagnostic biomarker and prognostic biomarker by influencing cell cycle, proliferation, migration, and invasion ability in hepatocellular carcinoma cell lines.

Hepatocellular carcinoma (HCC) is a lethal malignancy worldwide. HCC has traits of late diagnosis and high recurrence. This study explored potential diagnosis and prognosis significance of phospholipase C epsilon 1 (PLCE1) in HCC. The messenger RNA (mRNA) levels and diagnostic value of PLCE1 were determined by real-time polymerase chain reaction and online databases GEPIA, oncomine, and GSE14520 data set. Survival analysis used the Kaplan-Meier Plotter website. Cell cycle, proliferation, migration, and invasion assays were performed with downregulated PLCE1 expression in HCC-M and HepG2 cell lines. PLCE1 was differentially expressed and highly expressed in tumors and had low expression in nontumor tissues (all p < .05). The diagnostic value of PLCE1 was validated with the datasets (all p < .01, all areas under curves > 0.7). PLCE1 mRNA expression was associated with the overall and relapse-free survival (both p < .05). Functional experiments indicated that downregulation of PLCE1 expression led to increased G1 stage in cell cycle and decreased cell proliferation, migration, and invasion compared with a negative control group (all p ≤ .05). The oncogene PLCE1 was differentially expressed in HCC and non-HCC tissues. It is a candidate for diagnosis and serves as prognosis biomarker. PLCE1 influenced survival by affecting the cell cycle, proliferation, migration, and invasion ability.

Non-coding RNAs regulate autophagy process via influencing the expression of associated protein.

Autophagy is a tightly-regulated multi-step process involving the lysosomal degradation of proteins and cytoplasmic organelles. Central to this process is the formation of the autophagosome, a double membrane-bound vesicle, which is fuse with lysosomes or endosomes, and then deliver its cytoplasmic cargo to the lysosomes. Here, we summarize the recent process of autophagy, focusing on protein molecules, their complexes, and its essential roles of autophagy in various phases. Emerging evidence has revealed that miRNAs, lncRNAs, and circRNAs play an indispensable role in autophagy regulation by modulating targeting gene expression. This review we will summarize the main features of ncRNAs and point to gaps in our current knowledge of the connection between ncRNAs and autophagy, as well as their potential utilization in various disease phenotypes. Also, we highlight recent advances in ncRNAs and autophagy-associated protein interaction and how they regulate the autophagy process.