[Characterization and biological significance of peripheral blood dendritic cell subsets in patients with severe burn].

OBJECTIVE: To investigate the characteristics of dendritic cells (DCs) in patients with severe burn. METHODS: Peripheral blood samples were obtained from 12 patients with severe burn 1, 2, 3, and 4 weeks after burn, and from 10 healthy donors as controls. Mononuclear cells were isolated, and GM-CSF 1000 and IL-4 were used to induce the transformation of DCs. Mature DCs were harvested and underwent flow cytometry to detect the expression of HLA-DR, CD80, CD83, CD86, CD14, and CD11c. RESULT: 1, 2, 3, and 4 weeks after burn, the expression levels of HLA-DR were 62.1% +/- 8.4%, 65.0% +/- 6.2%, 68.4% +/- 5.7%, and 75.4% +/- 8.4% respectively; the expression levels of CD80 were 12.9% +/- 3.7%, 14.7% +/- 2.5%, 16.1% +/- 4.2%, and 16.2% +/- 4.8%; the expression levels of CD83 were 15.1% +/- 4.2%, 15.1% +/- 4.0%, 22.2% +/- 7.7%, and 21.3% +/- 7.0% respectively; the expression levels of CD86 were 69.2% +/- 7.1%, 70.5% +/- 5.4%, 75.1% +/- 6.1%, and 79.6% +/- 6.4% respectively, all significantly lower than that of the control group (84.3% +/- 8.2%, all P < 0.001). 1, 2, 3, and 4 weeks after burn, the expression levels of CD14 in the DCs of the severe burn patients were 12.7% +/- 1.9%, 12.0% +/- 1.5%, 11.3% +/- 1.3%, and 9.3% +/- 1.7% respectively, all significantly higher than that of the control group (7.3% +/- 1.5%, P < 0.001). 1, 2, 3, and 4 weeks after burn, the expression levels of CD11c in the DCs of the severe burn patients were 86.8% +/- 6.1%, 89.5% +/- 5.1%, 91.3% +/- 2.8%, and 89.4% +/- 4.0% respectively, a little lower than that of the control group, however, without significant difference (92.6% +/- 3.8%, P > 0.05). CONCLUSION: The functions of DCs in the patients with severe burn are declined, which may partly contribute to infection after burn.

[A new method of oxygen delivery to preventing and relieving the anoxia injury--an experimental study and clinical application of hyperoxygen compound sodium chloride solution in treatment of burn shock].

OBJECTIVE: To evaluate the clinic effects and prognosis of hyperoxygen compound sodium chloride solution (HCSCS) in treatment of burn shock. METHODS: (1) Severe burned patients with 50% - 69% TBSA were enrolled and randomly divided into 2 groups, treated by HCSCS and CSCS respectively. Indices such as PaO(2), Hb, Hct and complication were observed. (2) Severe burned patients with more than 70% TBSA were enrolled and indices above and lactate were investigated as well. RESULTS: (1) The cases treated with HCSCS showed relatively stabilization during shock stage, little complication, less liquor volume and remarkable higher oxygen pressure. (2) Severe burned patients with more than 70% TBSA had high level of PaO(2) and normal lactate and less complication. CONCLUSION: The early application of HCSCS is a relatively effective method in treatment of burn shock and favourable prognosis.