Activation of cGAS/STING Drives Inflammation and Cellular Senescence of Macrophages in Ovarian Endometrioma Induced by Endometriotic Cyst Fluid.

Ovarian endometrioma (OE) is a common gynecological condition characterized by the formation of "chocolate cysts". Recent research indicates that the cyst fluid acts as a "toxic environment" for the ovary and plays a significant role in the development of OE, with macrophages being pivotal. However, the specific molecular and cellular mechanisms of it are not fully understood. In this study, clinical samples are integrated, single-cell sequencing, in vivo and in vitro experimental models to comprehensively investigate the effects of OE fluid on ovarian function and the mechanisms of it. Combined with bioinformatics analysis and experimental validation, the findings demonstrate that OE fluid can cause ovarian function decline, which associated with inflammatory response, and mitochondrial dysfunction and cellular senescence, while activating the cGAS/STING signaling pathway. As a STING inhibitor, H-151 effectively alleviates ovarian dysfunction, inflammatory state and cell apoptosis induced by OE fluid. Furthermore, it is also discovered that H-151 can inhibit OE fluid-induced mitochondrial dysfunction and cellular senescence. These findings provide important theoretical and experimental foundations for further research and development of STING inhibitors as potential drugs for treating ovarian dysfunction.

Observing two-photon subwavelength interference of broadband chaotic light in a polarization-selective Michelson interferometer.

We demonstrated a method to achieve the two-photon subwavelength effect of true broadband chaotic light in polarization-selective Michelson interferometer based on two-photon absorption detection. To our knowledge, it is the first time that this effect has been observed with broadband chaotic light. In theory, the two-photon polarization coherence matrix and probability amplitudes matrix are combined to develop polarized two-photon interference terms, which explains the experimental results well. To make better use of this interferometer to produce the subwavelength effect, we also make a series of error analyses to find out the relationship between the visibility and the degree of polarization error. Our experimental and theoretical results contribute to the understanding of the two-photon subwavelength interference, which shed light on the development of the two-photon interference theory of vector light field based on quantum mechanics. The characteristic of the two-photon subwavelength effect have significant applications in temporal ghost imaging, such as it helps to improve the resolution of temporal objects.

Controllable superbunching effect from four-wave mixing process in atomic vapor.

Correlation property of light limits the performance in related applications such as the visibility of ghost imaging or intensity interferometry. To exceed these performance limits, we here manipulate the degree of second- and higher-order coherence of light by changing controllable variables in four-wave mixing (FWM) process. The measured degree of second- and third-order coherence of the output light beams considerably exceed those of the incident pseudothermal light. Namely superbunching effects, g(2)(0) value up to 7.47 and g(3)(0) value up to 58.34, are observed experimentally. In addition, strong second- and third-order cross-correlation exist between the output light beams. Further insights into the dependence of superbunching light on the temperature of Rb vapor, the laser detuning and the optical power of all the incident light beams show that it can serve as a light source with a tunable superbunching degree.