Sonoactivated Cascade Fenton Reaction Enhanced by Synergistic Modulation of Electron-Hole Separation for Improved Tumor Therapy.

Chemodynamic therapy (CDT) is an emerging targeted treatment technique for tumors via the generation of highly cytotoxic hydroxyl radical (·OH) governed by tumor microenvironment-assisted Fenton reaction. Despite high effectiveness, it faces limitations like low reaction efficiency and limited endogenous H2 O2 , compromising its therapeutic efficacy. This study reports a novel platform with enhanced CDT performance by in situ sono-activated cascade Fenton reaction. A piezoelectric g-C3 N4 (Au-Fe-g-C3 N4 ) nanosheet is developed via sono-activated synergistic effect/H2 O2 self-supply mediated cascade Fenton reaction, realizing in situ ultrasound activated cascade Fenton reaction kinetics by synergistic modulation of electron-hole separation. The nanosheets consist of piezoelectric g-C3 N4 nanosheet oxidizing H2 O to highly reactive H2 O2 from the valence band, Fe3+ /Fe2+ cycling activated by conduction band to generate ·OH, and Au nanoparticles that lower the bandgap and further adopt electrons to generate more 1 O2 , resulting in improved CDT and sonodynamic therapy (SDT). Moreover, the Au-Fe-g-C3 N4 nanosheet is further modified by the targeted peptide to obtain P-Au-Fe-g-C3 N4 , which inhibits tumor growth in vivo effectively by generating reactive oxygen species (ROS). These results demonstrated that the sono-activated modulation translates into a high-efficiency CDT with a synergistic effect using SDT for improved anti-tumor therapy.

Acid vacuolar invertase 1 (PbrAc-Inv1) and invertase inhibitor 5 (PbrII5) were involved in sucrose hydrolysis during postharvest pear storage.

In pear, sucrose was mainly distributed in vacuole; and the alternation of sucrose abundance was associated the change of vacuolar invertase (VI) activity during fruit storage. However, the molecular mechanism beneath such phenomenon has not been clarified until recently. For this, a combination of metabolite, enzyme activity, transcriptome, quantitative real-time PCR (qRT-PCR), bioinformation, subcellular localization, and transient overexpression assay was conducted in this study to identify the acid invertase 1 (PbrAc-Inv1) and invertase inhibitor 5 (PbrII5) involved in sucrose degradation during 'Housui' pear storage. Both PbrAc-Inv1 and PbrII5 were located in vacuolar membrane. PbrAc-Inv1 could accelerate sucrose degradation; on the other hand, PbrII5 could bind with PbrAc-Inv1 to form a inactive complex, downregulate the VI activity, and suppressed sucrose decomposition. Based on Bio-layer interferometry (BLI) result after domain substitution, the domain on the left of catalytic 'WEC-P/V-D' box in PbrAc-Inv1 might played a key role in its interaction with PbrII5.

[Professor ZHANG Yu-Lian's experiences in treatment of post-stroke ataxia by regulating marrow sea acupuncture].

The experiences and characteristics of professor ZHANG Yu-lian in regulating marrow sea acupuncture for treatment of post-stroke ataxia is introduced. Professor ZHANG established regulating marrow sea acupuncture based on the fundamentals of traditional Chinese medicine combined with brain functional region projection and cerebrovascular distribution of modern medicine. "Three acupoints regulating balance" "3 acupoints regulating transportation" "3 acupoints regulating tremor" and Fengchi (GB 20), Fengfu (GB 16), Wangu (GB 12), Tianzhu (BL 10) and C3-C6 Jiaji (Ex-B2) acupoints were applied in this kind of manipulation. We combined holding spirits of doctor and patient together, and controlled the sensation transduction with different manipulations to reach the foci, which emphasis on the doctor-patient cooperation and body-mind co-regulation, finally promote patients' comprehensive rehabilitation.