Taurine Inhibits Lung Metastasis in Triple-Negative Breast Cancer by Modulating Macrophage Polarization Through PTEN-PI3K/Akt/mTOR Pathway.

SUMMARY: Taurine (Tau) has been found to inhibit triple-negative breast cancer (TNBC) invasion and metastasis. However, its effect on tumor-promoting macrophages and tumor suppressor macrophages in breast cancer progression remains unknown. In this study, we investigated the effects of Tau on macrophage polarization and its role in TNBC cell growth, invasion, and metastasis. We induced human THP-1 monocytes to differentiate into M2 macrophages through exogenous addition of interleukin-4. We used the TNBC cell lines MDA-MB-231 and BT-549 cultured in a conditioned medium from M2 macrophages to investigate the effect of Tau on tumor growth and invasion. We analyzed macrophage subset distribution, M1 and M2 macrophage-associated markers, and mRNA expression by quantitative polymerase chain reaction. We also detected the PTEN-PI3K/Akt/mTOR signaling pathway that mediates M1 macrophage to suppress tumor invasion using western blotting. Our results showed that Tau inhibits breast cancer metastasis to the lungs in vivo and cell invasion by altering the polarization of tumor-associated macrophage in vitro. In addition, Tau can up-regulate PTEN expression, suppress the PI3K-Akt signaling pathway, and promote the M1 polarization of macrophages, which ultimately inhibits the metastasis of TNBC cells. Our findings suggest that Tau inhibits the activation of the PI3K-Akt-mTOR signaling pathway by up-regulating PTEN, promotes the proportion of M1 macrophages in tumor-associated macrophage, and suppresses the invasion and metastasis of TNBC. This provides a potential therapeutic approach to influence cancer progression and metastasis.

High Tribocatalytic Performance of FeOOH Nanorods for Degrading Organic Dyes and Antibiotics.

Tribocatalysis is vitally important for electrochemistry, energy conservation, and water treatment. Exploring eco-friendly and low-cost tribocatalysts with high performance is crucial for practical applications. Here, the highly efficient tribocatalytic performance of FeOOH nanorods is reported. The factors related to the tribocatalytic activity such as nanorod diameter, surface area, and surface roughness are investigated, and the diameter of the FeOOH nanorods is found to have a significant effect on their tribocatalytic performance. As a result, under ultrasonic excitation, the optimized FeOOH nanorods exhibit superior tribocatalytic degradation toward rhodamine B (RhB), acid orange 7, methylene blue, methyl orange dyes, and their mixture. The RhB and mixed dyes are effectively degraded within 20 min (k = 0.179 min-1 ) and 35 min (k = 0.089 min-1 ), respectively, with the FeOOH nanorods showing excellent reusability. Moreover, antibiotics, such as tetracycline hydrochloride, phenol, and bisphenol A are efficiently degraded. Investigation of the catalytic mechanism reveals that the friction-generated h+ as well as these yielded •OH and •O2 - active radicals participate in the catalytic reaction. This work not only shed light on the design of high-performance tribocatalyst but also demonstrates that by harvesting mechanical energy, the FeOOH nanorods are promising materials for removing organic contaminants in wastewater.

Comprehensive analysis of pigment alterations and associated flavor development in strip and needle green teas.

Strip/needle green teas (SGT/NGT) processed using innovative technologies are in high demand; however, mechanisms behind their color and flavor have not been comprehensively studied. We aimed to reveal the dynamics of major pigmented components (carotenoids, lipids, flavonoids, and Maillard products) and their contributions to the flavor of green teas. The total content of flavonoids in SGT and NGT were 255 ± 4.51 and 201 ± 3.91 mg·g-1, respectively; these values are slightly lower than that in fresh leaves (FLs), resulting in a fresh and sweet aftertaste. In average, carotene content in SGT/NGT (24.8 µg·g-1) was higher than in FL (17.4 µg·g-1), whilst xanthophyll content (603 µg·g-1) decreased to one-half of that in FL (310 µg·g-1). Among the 218 primary metabolites, glutamine, glutamic acid, and arginine were found to accumulate and were dominate contributors for the umami and sweet taste. Notably, more than 96 volatiles were screened and revealed their correlations with carotenoids, lipids, and amino acids. Overall, the synergism between pigments and their non-enzymatic derivates' contribution to GT characterized flavor was illustrated.

Synthesis and physicochemical properties of an aromatic chitosan derivative: In vitro antibacterial, antioxidant, and anticancer evaluations, and in silico studies.

This study was designed to synthesize a functionalized chitosan by coupling the amine groups of chitosan with 2,4,6-Trimethoxybenzaldehyde, producing a chitosan Schiff base (Cs-TMB). The development of Cs-TMB was verified employing FT-IR, 1H NMR, the electronic spectrum, and elemental analysis. Antioxidant assays exhibited significant ameliorations of Cs-TMB, reporting scavenging activities of 69.67 ± 3.48 % and 39.65 ± 1.98 % for ABTS•+ and DPPH, respectively, while native chitosan showed scavenging ratios of 22.69 ± 1.13 % and 8.24 ± 0.4.1 % toward ABTS•+ and DPPH, respectively. Besides, Cs-TMB exerted significant antibacterial activity up to 90 % with remarkable bactericidal capacity against virulent gram-negative and gram-positive bacteria compared to the original chitosan. Furthermore, Cs-TMB exhibited a safe profile against normal fibroblast cells (HFB4). Interestingly, flow cytometric analysis showed that Cs-TMB demonstrated prominent anticancer properties of 52.35 ± 2.99 % against human skin cancer cells (A375), compared to 10.66 ± 0.55 % for Cs-treated cells. Moreover, Python and PyMOL in-house scripts were used to predict the interaction of Cs-TMB with the adenosine A1 receptor and visualized as a protein-ligand system submerged in a lipid membrane. Overall, these findings accentuate that Cs-TMB could be a favorable representative for wound dressing formulations and skin cancer treatment.

RC Bridge Oscillation Memristor Chaotic Circuit for Electrical and Electronic Technology Extended Simulation Experiment.

The fourth basic circuit component, the memristor, has been proposed for a long time, but it is not mentioned in the experiment teaching system of Electrical and Electronic Technology. In this paper, an RC bridge oscillation chaotic circuit based on memristor is designed to solve this problem. The dynamical behavior of the circuit system is analyzed using Lyapunov exponents spectrum, bifurcation diagram, phase portrait and Poincaré map. A series of complex dynamical behaviors such as symmetric single-scroll coexistence, asymmetrical single-scroll coexistence, symmetric double-scroll coexistence and asymmetrical limit-cycle coexistence exist in the circuit system. This research plays a critical role in enriching students' knowledge and improving the experiment teaching system of Electrical and Electronic Technology.

Technological parameter optimization for walnut shell-kernel winnowing device based on neural network.

The detection method for technological parameter is outdates as the traditional test cycle is long as well as the measurement error and the test amount are huge. Moreover, it is difficult to disclose the operation mechanism of devices as the operation is time-consuming and laborious. Therefore, numerical simulation was used in this study to reveal the mechanism of the walnut shell-kernel winnowing device. Moreover, the influence of baffle opening combinations, inlet wind velocity and inlet angle on cleaning rate and loss rate was predicted by the neural network model. The results demonstrated that inlet wind velocity was the primary influencing factor of cleaning rate, followed by baffle opening and inlet angle. Besides, inlet wind velocity was the primary influencing factor of loss rate, followed by inlet angle and baffle opening. The winnowing device performed best (79.91% cleaning rate, 14.37% loss rate) when the baffle opening, inlet wind velocity and inlet angle were 7.01 cm, 24.36 m/s, and 9.47°. In addition, 1/8 walnut shells and 1/4 walnut kernels were incorrectly classified due to the increase in inlet wind velocity. The inlet wind velocity was considered the major cause behind the deteriorating winnowing performance of the device. Finally, the bench test and simulation optimization results were compared. The cleaning rate and loss rate relative error during the simulation test was lower than 1.06%, which ascertained the feasibility and validity of the neural network as well as the combined numerical simulation method. This study could be useful for future research and development of shell-kernel winnowing devices for hard nuts.

Sm-Doped PIN-PMN-PT Transparent Ceramics with High Curie Temperature, Good Piezoelectricity, and Excellent Electro-Optical Properties.

Transparent piezoelectric materials are capable of coupling several physical effects such as optics, acoustics, electricity, and mechanical deformation together, which expands applications for mechanical-electro-optical multifunctional devices. However, piezoelectricity, transparency, and Curie temperature restrict each other, so it is difficult to achieve high piezoelectricity with both good transparency and a high Curie point. In this paper, Sm-doped 24Pb(In1/2Nb1/2)O3-42Pb(Mg1/3Nb2/3)O3-34PbTiO3 (PIN-PMN-PT) transparent ceramic with a high piezoelectric coefficient of 905 pC/N, excellent electro-optical coefficient of 814 pm/V, and high Curie-point of 179 °C is fabricated. Sm doping effect on the phase structures, piezoelectricity, ferroelectricity, optical transparency, electro-optical properties, and thermal stability is systematically investigated. Compared with PMN-PT transparent ceramics, PIN-PMN-PT transparent ceramics exhibit better temperature stability. Electro-optical modulation and energy conversion are achieved using PIN-PMN-PT transparent piezoelectric ceramic, which indicates that it has great potential to develop mechanical-electrical-optical multifunctional coupling devices for optical communication, energy harvesting, photoacoustic imaging, and so on.

The Correlations Between Serum Hcy Level and Seizures and Cognitive Function in Patients After Stroke.

BACKGROUNDS: Post-stroke cognitive dysfunction (PSCI), a set of illnesses ranging from moderate cognitive impairment to dementia, which is one of the most prevalent consequences following a stroke. Homocysteine (Hcy) has been related to a number of neurological and systemic diseases. It's also a known risk factor for cardiovascular disease and systemic atherosclerosis (CVD). The link between Hcy and PSCI, on the other hand, is unknown. METHODS: Our hospital evaluated 325 patients with acute cerebral infarction between January 1, 2018 and December 1, 2021. There are biological markers and baseline data available. Patients were divided into two groups based on the results of the Montreal Cognitive Assessment (MoCA). The researchers performed logistic regression analysis to find variables that may be linked to PSCI. RESULTS: HCY levels were significantly higher in PSCI patients than in non-PSCI patients. Age, education, seizure manifestation, and income level were all shown to be independent risk variables for PSCI in a multivariate logistic analysis. Hcy levels in PSCI patients differed considerably between the high and low groups. The high and low Hcy levels groups had significantly varied hypertension histories and urine levels. Hcy levels in PSCI patients differed considerably between the high and low groups. The high and low Hcy levels groups had significantly varied hypertension histories and urine levels. CONCLUSION: Serum Hcy levels have been linked to PSCI in post-stroke patients, and researchers believe that serum Hcy levels will diminish PSCI.

Safety Risk Estimation of Construction Project Based on Energy Transfer Model and System Dynamics: A Case Study of Collapse Accident in China.

Analyzing and understanding the occurrence and evolution mechanisms of construction accidents are important for construction safety management. This study proposed a hybrid approach of integrating the energy transfer model (ETM) and system dynamics (SD) theory to delineate the entire evolution stage of the construction accident. Specifically, the Fengcheng Power Plant construction platform collapse accident (FPCA) was taken as a practical case study. First, the ETM is applied to demonstrate the evolving nature of the accident. Then, the network of the accident-causing factors is constructed using the SD theory to analyze the dynamic change characteristics. The results indicate that the accident was caused by risk factors with complex interactions at the management level. An energy constraint failure occurred when the transfer of dangerous energy transpired at the physical entity level, inducing the event. The proposed approach can provide a useful reference for safety risk estimation and management in future major construction projects.

Use of covered stents to treat complex cerebrovascular diseases: Expert consensus.

The treatment of complex cerebrovascular diseases (CCVDs) at the skull base, such as complex intracranial aneurysms, carotid-cavernous sinus fistulas, and intracranial artery traumatic injuries, is a difficult clinical problem despite advances in endovascular and surgical therapies. Covered stents or stent graft insertion is a new concept for endovascular treatment that focuses on arterial wall defect reconstruction, differing from endovascular lesion embolization or flow diverter therapies. In recent years, covered stents specifically designed for cerebrovascular treatment have been applied in the clinical setting, allowing thousands of patients with CCVDs to undergo intraluminal reconstruction treatment and achieving positive results, even in the era of flow diverters. Since there is no unified reference standard for the application of covered stents for treating CCVDs, it is necessary to further standardize and guide the clinical application of this technique. Thus, we organized authoritative experts in the field of neurointervention in China to write an expert consensus, which aims to summarize the results of covered stent insertion in the treatment of CCVDs and propose suitable standards for its application in the clinical setting. Based on the contents of this consensus, clinicians can use individualized intraluminal reconstruction treatment techniques for patients with CCVDs.

In situ TEM observations of growth mechanisms of PbO nanoparticles from a Sm-doped PMN-PT matrix.

An excess PbO is usually added to raw materials to compensate for PbO volatilization during high-temperature sintering of a (1 - x)Pb(Mg1/3Nb2/3)O3-xPbTiO3 (PMN-PT) piezoelectric material. However, the detailed growth mechanism of liquid phase and solid phase PbO due to excess PbO during the sintering process is still unknown. Here, the evolution behavior and growth mechanism of PbO nanoparticles from a Sm-doped 0.70PMN-0.30PT (Sm-PMN-PT) matrix were in situ observed using transmission electron microscopy with the help of electron beam irradiation. It was found that PbO nanodroplets firstly separated from the Sm-PMN-PT matrix, leading to rapid growth of newly formed PbO nanodroplets. Then, these nanodroplets coalesced into solid phase PbO nanoparticles with their size increased. After that, small solid phase nanoparticles further grew into large PbO nanoparticles by either rapidly engulfing adjacent nanodroplets and nanoparticles or slowly merging by matching these same crystal planes of adjacent nanoparticles. Finally, a heterojunction was formed between the formed large PbO nanoparticles and Sm-PMN-PT matrix. Our investigations demonstrate that the excess PbO could provide a liquid environment at the interface of Sm-PMN-PT, and the PbO nanoparticles formed act as the secondary phase at the grain boundaries of the Sm-PMN-PT matrix. This work provides a deep understanding of the role of excess PbO in the synthesis of lead-based piezoelectric materials.

Effects of Municipal Solid Waste on Planting Properties and Scouring Resistance of Vegetation Concrete (Wuhan, China).

Vegetation concrete (VC) laid as a reinforcement base and covered by a soil layer with vegetation has been increasingly used to beautify the landscape, reduce environmental pollution and control stormwater runoff. In this study, the effects of municipal solid waste (MSW) on vegetation characteristics of modified VC were tested under different mix compositions. We first explored the effects of the mixed concrete environment on Festuca elata and perennial Ryegrass for 60 days. Then, the influence of various MSW contents added to different percentages of cement on scouring resistance of VC was examined. The experimental results revealed that the germination rates and plant heights of both species decreased with the increase in concrete content. Considering the scouring resistances, the optimal mix proportion of MSW-modified VC was recommended as No. 25, with 5% KW fertilizer, 8% cement and 0.5% wheat straw in this study. Furthermore, adding a small amount of fallen leaves or silica fume to VC can promote the growth of both species to some extent, although these additions had an inverse effect on the scouring resistances. The results contribute to beneficial knowledge for future research on the feasibility of the use these species with VC technology for slope ecological restoration.

Construction of an egg-like DTAB/SiO2 composite for the enhanced removal of uranium.

For the past few years, the environmental safety problems of radioactive nuclides caused wide public concern. In this work, the dodecyl trimethyl ammonium bromide-modified silicon dioxide composite (DTAB/SiO2) was synthesized for the elimination of uranium. The dodecyl trimethyl ammonium bromide can decorate the surface of the silicon dioxide and change its surface topography, which can offer more active sites and functional groups for the combination of U(VI). The removal capacity of U(VI) on DTAB/SiO2 reached 78.1 mg/g, which was greater than that of the silicon dioxide nanopowder. In the adsorption process, the surface oxygen-containing functional groups formed surface complexation with uranium. The results may provide helpful content to eliminate U(VI) and expand the application of surfactant in radioactive nuclide cleanup.

Value of T2 Mapping in the Dynamic Evaluation of Renal Ischemia-Reperfusion Injury.

OBJECTIVE: To explore the value of T2 mapping in the dynamic quantitative evaluation of renal ischemia- reperfusion injury (IRI). MATERIALS AND METHODS: Forty-eight healthy New Zealand rabbits were randomly divided into IRI group (n = 40) and control group (n = 8). Rabbits in the IRI group underwent left renal artery clamping for 60 minutes. Rabbits underwent MRI examinations (T2WI and T2 mapping) before and 1, 12, 24, and 48 hours after IRI. The inter-observer and intra-observer reproducibility of the T2 values were assessed using the intraclass correlation coefficient (ICC) with 95% confidence interval (CI). Correlations between the T2 value of the renal outer medulla and injury scores were assessed by Spearman correlation analysis. The repeated measures analysis of variance was used to compare the differences in T2 values of the IRI and control group across the different time points. RESULTS: Both of the intra-observer (ICC = 0.97, 95% CI 0.95-0.99) and inter-observer reproducibility (ICC = 0.92, 95% CI 0.86-0.96) were excellent for T2 values. The T2 value of the renal outer medulla was moderately positive correlated with tubular epithelial edema (ρ = 0.686, p < 0.001). In IRI group, T2 values of the renal outer medulla were increase at 1 h after IRI (p = 0.001) and were decrease from 1 h to 12 h (p = 0.002). At 1 h after IRI, the T2 values of the renal outer medulla for the IRI group were higher than those for the control group (p < 0.001). CONCLUSION: T2 mapping can reflect the dynamic changes of renal parenchyma in an animal model of IRI and be used to assess the early renal IRI.

Highly Transparent Eu-Doped 0.72PMN-0.28PT Ceramics with Excellent Piezoelectricity.

The 0.975[0.72Pb(Mg1/3Nb2/3)O3-0.28PbTiO3]-0.025Eu2O3 ceramics were prepared by a two-step sintering process including oxygen sintering and hot-pressing. An ultrahigh piezoelectric charge coefficient of 1400 pC/N and a superior optical transmittance up to 68% were simultaneously achieved. The underlying mechanism was discussed from a microstructural perspective, where the watermark domain configuration with a small domain size is responsible for the high optical transmission, while the large remanent polarization and dielectric constant and the introduced tetragonal phase with a parallel stripe domain structure are believed to synergistically contribute to the high piezoelectric coefficients. This work demonstrates that the rare-earth dopant in the PMN-PT ceramic system is conducive to enhanced transparency and piezoelectricity.

Mid-infrared ridge waveguides fabricated in CaF2 crystal by the technique of O5+ ion irradiation.

The fabrication of ridge waveguides in calcium fluoride (CaF2) crystal working at the mid-infrared wavelength was studied. First, the planar waveguide was fabricated by using O5+ ion irradiation, and then the ridge waveguide structure was manufactured by precise diamond blade dicing. The propagation loss was measured by end-face coupling arrangement, and then annealing treatment was implemented to optimize the waveguide performance, and the propagation loss was finally reduced to 0.5 dB/cm. We measured the Raman spectra of the waveguide and substrate to observe the damage to the material lattice caused by O5+ ion irradiation technology.

Reacquainting the Electrochemical Conversion Mechanism of FeS2 Sodium-Ion Batteries by Operando Magnetometry.

In spite of the excellent electrochemical performance in lithium-ion batteries (LIBs), transition-metal compounds usually show inferior capacity and cyclability in sodium-ion batteries (SIBs), implying different reaction schemes between these two types of systems. Herein, coupling operando magnetometry with electrochemical measurement, we peformed a comprehensive investigation on the intrinsic relationship between the ion-embedding mechanisms and the electrochemical properties of the typical FeS2/Na (Li) cells. Operando magnetometry together with ex-situ transmission electron microscopy (TEM) measurement reveal that only part of FeS2 is involved in the conversion reaction process, while the unreactive parts form "inactive cores" that lead to the low capacity. Through quantification with Langevin fitting, we further show that the size of the iron grains produced by the conversion reaction are much smaller in SIBs than that in LIBs, which may lead to more serious pulverization, thereby resulting in worse cycle performance. The underlying reason for the above two above phenomena in SIBs is the sluggish kinetics caused by the larger Na-ion radius. Our work paves a new way for the investigation of novel SIB materials with high capacity and long durability.

Can R2 ' mapping evaluate hypoxia in renal ischemia reperfusion injury quantitatively? An experimental study.

PURPOSE: To explore if R2 ' mapping can assess renal hypoxia in rabbits with ischemia reperfusion injury (IRI). METHODS: Forty rabbits were randomly divided into 4 groups according to the clipping time: the sham group and 45 min, 60 min, and 75 min for the mild, moderate, and severe groups (with n = 10 each group), respectively. Intravenous furosemide (FU) was administered 24 h after IRI. All rabbits were performed 5 times (IRIpre , IRI24h , FU5min , FU12min , and FU24min ) with a 3.0 Tesla MR. The R2 ' values and the hypoxic scores were then recorded. The repeated measurement analysis of variance and Spearman correlation analysis was used for statistical analysis. RESULTS: Compared to the baseline, the medullary R2 ' values increased significantly 24 h after the IRI (baseline 19.31 ± 1.21 s-1 , mild group 20.05 ± 1.26 s-1 , moderate group 25.38 ± 1.38 s-1 , and severe group 25.79 ± 1.10 s-1 ; each P < .001). FU led to a significant decrease in the medullary R2 ' value (sham group 11.17 ± 4.33 s-1 , mild group 7.80 ± 0.74 s-1 , moderate group 3.92 ± 0.28 s-1 , and severe group 3.82 ± 0.23 s-1 ; each P < .05). Quantitative hypoxic scores revealed significant differences among the 4 groups in the outer medulla (P < .001 each). The medullary R2 ' differences (before and after intravenous FU) were significantly correlated with the hypoxic scores, respectively (P < .001). CONCLUSION: R2 ' mapping can evaluate the renal hypoxia in the procession of IRI in rabbits and might serve as a quantitative biomarker for IRI.

Architecting Hierarchical WO3 Agglomerates Assembled With Straight and Parallel Aligned Nanoribbons Enabling High Capacity and Robust Stability of Lithium Storage.

The pursuit of electrochemical energy storage has led to a pressing need on materials with high capacities and energy densities; however, further progress is plagued by the restrictive capacity (372 mAh g-1) of conventional graphite materials. Tungsten trioxide (WO3)-based anodes feature high theoretical capacity (693 mAh g-1), suitable potential, and affordable cost, arousing ever-increasing attention and intense efforts. Nonetheless, developing high-performance WO3 electrodes that accommodate lithium ions remains a daunting challenge on account of sluggish kinetics characteristics and large volume strain. Herein, the well-designed hierarchical WO3 agglomerates assembled with straight and parallel aligned nanoribbons are fabricated and evaluated as an anode of lithium-ion batteries (LIBs), which exhibits an ultra-high capacity and excellent rate capability. At a current density of 1,000 mA g-1, a reversible capacity as high as 522.7 mAh g-1 can be maintained after 800 cycles, corresponding to a high capacity retention of ∼80%, demonstrating an exceptional long-durability cyclic performance. Furthermore, the mechanistic studies on the lithium storage processes of WO3 are probed, providing a foundation for further optimizations and rational designs. These results indicate that the well-designed hierarchical WO3 agglomerates display great potential for applications in the field of high-performance LIBs.

A nondestructive testing method for soluble solid content in Korla fragrant pears based on electrical properties and artificial neural network.

The detection of soluble solid content in Korla fragrant pear is a destructive and time-consuming endeavor. In effort to remedy this, a nondestructive testing method based on electrical properties and artificial neural network was established in this study. Specifically, variations of electrical properties (e.g., equivalent parallel capacitance, quality factor, loss factor, equivalent parallel resistance, complex impedance, and equivalent parallel inductance) of Korla fragrant pears with accumulated temperature were tested using a workbench developed by ourselves. After that the characteristic variables of electrical properties were constructed by principal component analysis (PCA). In addition, three models were constructed to predict SSC in Korla fragrant pears based on the characteristic variables: general regression neural network (GRNN), back-propagation neural network (BPNN), and adaptive network fuzzy inference system (ANFIS). The results indicated that the GRNN model has the best prediction effects of SSC (R 2 = 0.9743, RMSE = 0.2584), superior to that of the BPNN and ANFIS models. Results facilitate a successful, alternative application for rapid assessment of SSC of the maturation stage Korla fragrant pear.