State of Play of Critical Mineral Based Catalysts for Electrochemical E-Refinery to Synthetic Fuels.

The pursuit of decarbonization involves leveraging waste CO2 for the production of valuable fuels and chemicals (e.g., ethanol, ethylene, and urea) through the electrochemical CO2 reduction reactions (CO2RR). The efficacy of this process heavily depends on electrocatalyst performance, which is generally reliant on high loading of critical minerals. However, the supply of these minerals is susceptible to shortage and disruption, prompting concerns regarding their usage, particularly in electrocatalysis, requiring swift innovations to mitigate the supply risks. The reliance on critical minerals in catalyst fabrication can be reduced by implementing design strategies that improve the available active sites, thereby increasing the mass activity. This review seeks to discuss and analyze potential strategies, challenges and opportunities for improving catalyst activity in CO2RR with a special attention to addressing the risks associated with critical mineral scarcity. By shedding light onto these aspects of critical mineral-based catalyst systems, we aim to inspire the development of high-performance catalysts and facilitate the practical application of CO2RR technology, whilst mitigating adverse economic, environmental, and community impacts. This article is protected by copyright. All rights reserved.

Tamoxifen upregulates the peroxisomal ß-oxidation enzyme Enoyl CoA hydratase and 3-hydroxyacyl CoA hydratase ameliorating hepatic lipid accumulation in mice.

Tamoxifen is an estrogen receptor modulator that has been reported to alleviate hepatic lipid accumulation in mice, but the mechanism is still unclear. Peroxisome fatty acid ß-oxidation is the main metabolic pathway for the overload of long-chain fatty acids. As long-chain fatty acids are a cause of hepatic lipid accumulation, the activation of peroxisome fatty acid ß-oxidation might be a novel therapeutic strategy for metabolic associated fatty liver disease. In this study, we investigated the mechanism of tamoxifen against hepatic lipid accumulation based on the activation of peroxisome fatty acid ß-oxidation. Tamoxifen reduced liver long-chain fatty acids and relieved hepatic lipid accumulation in high fat diet mice without sex difference. In vitro, tamoxifen protected primary hepatocytes against palmitic acid-induced lipotoxicity. Mechanistically, the RNA-sequence of hepatocytes isolated from the liver revealed that peroxisome fatty acid ß-oxidation was activated by tamoxifen. Protein and mRNA expression of enoyl CoA hydratase and 3-hydroxyacyl CoA hydratase were significantly increased in vivo and in vitro. Small interfering RNA enoyl CoA hydratase and 3-hydroxyacyl CoA hydratase in primary hepatocytes abolished the therapeutic effects of tamoxifen in lipid accumulation. In conclusion, our results indicated that tamoxifen could relieve hepatic lipid accumulation in high fat diet mice based on the activation of enoyl CoA hydratase and 3-hydroxyacyl CoA hydratase-mediated peroxisome fatty acids ß-oxidation.

Development and evaluation of short-form version of the Constitution in Chinese Medicine Questionnaire: study a new and best brief instrument of Chinese medicine for health management.

BACKGROUND: More efficient instruments for body constitution identification are needed for clinical practice. We aimed to develop the short-form version of the Constitution in Chinese Medicine Questionnaire (CCMQ) and evaluate for health management. METHODS: First, the short forms were developed through expert survey, classical test theory (CTT), and modern item response (IRT) based on the CCMQ. A combination of e-mail and manual methods was used in expert survey. Then, five indexes of CTT including criteria value-critical ratio, correlation coefficient, discrete tendency, internal consistency, and factor loading were used. And, IRT method was used through analyzing the discrimination and difficulty parameters of items. Second, the three top-ranked items of each constitution scale were selected for the simplified CCMQ, based on the three combined methods of different conditions and weights. Third, The psychometric properties such as completion time, validity (Construct, criterion, and divergent validity), and reliability (test-retest and internal consistency reliability) were evaluated. Finally, the diagnostic validity of the best short-form used receiver operating characteristic (ROC) curve. RESULTS: Three short-form editions were developed, and retained items 27, 23 and 27, which are named as WangQi nine body constitution questionnaire of Traditional Chinese Medicine (short-form) (SF-WQ9CCMQ)- A, B, and C, respectively. SF-WQ9CCMQ- A is showed the best psychometric property on Construct validity, Criterion validity, test-retest reliability and internal consistency reliability. The diagnostic validity indicated that the area under the ROC curve was 0.928 (95%CI: 0.924-0.932) for the Gentleness constitution scale, and were 0.895-0.969 and 0.911-0.981 for unbalance constitution scales using the cut-off value of the original CCMQ as 40 ("yes" standard) and 30 ("tendency" standard), respectively. CONCLUSIONS: Our study successfully developed a well short-form which has good psychometric property, and excellent diagnostic validity consistent with the original. New and simplified instrument and opportunity are provided for body constitution identification, health management and primary care implementation.

Discovery of a potent inhibitor targeting the cap-binding domain of the PB2 subunit of influenza RNA-dependent RNA polymerase.

Influenza pandemics have emerged as a significant global public health and security concern. PB2, a crucial subunit of the influenza RNA-dependent RNA polymerase (RdRP), has been identified as a promising target for influenza treatment. We herein report the discovery of a potent novel PB2 inhibitor, 7-51A, with a KD value of 1.64 nM as determined by ITC. The high activity of 7-51A was elucidated by the co-crystal structure of the PB2-7-51A complex, and comparative analysis revealed unique interactions that had never been observed before. The preliminary pharmacological evaluation indicated that 7-51A exhibited commendable cellular safety, hepatic microsomal metabolic safety and stability. Collectively, 7-51A was found to be an effective PB2 inhibitor and could be used as a lead compound for further studies.

A highly selective self-powered sensor based on the upconversion nanoparticles/CdS nanospheres for chlorpyrifos detection.

Light sources are crucial for photoelectrochemical (PEC) self-powered sensing, where visible light is widely used. However, due to its high energy, it has some downsides as an irradiation source for overall system, so it is urgent to achieve effective near-infrared (NIR) light absorption because it makes up a significant portion of the solar spectrum. Herein, up-conversion nanoparticles (UCNPs) that could increase the energy of low-energy radiation were combined with semiconductor CdS as the photoactive material (UCNPs/CdS), which broadens the response range of solar spectrum. The NIR light-excited self-powered sensor could be produced via oxidizing H2O at photoanode and lowering dissolved oxygen at cathode under the NIR light without external voltage. Meanwhile, molecularly imprinted polymer (MIP) was added to photoanode as a recognition element to increase the sensor's selectivity. The open-circuit voltage of the self-powered sensor grew linearly as chlorpyrifos concentration climbed from 0.01 to 100 ng mL-1, showing good selectivity as well as reproducibility. This work provides valuable basis for the preparation of efficient and practical PEC sensor with NIR light response.

Periodic Acid Modification of Chemical-Bath Deposited SnO2 Electron Transport Layers for Perovskite Solar Cells and Mini Modules.

Chemical bath deposition (CBD) has been demonstrated as a remarkable technology to fabricate high-quality SnO2 electron transport layer (ETL) for large-area perovskite solar cells (PSCs). However, surface defects always exist on the SnO2 film coated by the CBD process, impairing the devices' performance. Here, a facile periodic acid post-treatment (PAPT) method is developed to modify the SnO2 layer. Periodic acid can react with hydroxyl groups on the surface of SnO2 films and oxidize Tin(II) oxide to Tin(IV) oxide. With the help of periodic acid, a better energy level alignment between the SnO2 and perovskite layers is achieved. In addition, the PAPT method inhibits interfacial nonradiative recombination and facilitates charge transportation. Such a multifunctional strategy enables to fabricate PSC with a champion power conversion efficiency (PCE) of 22.25%, which remains 93.32% of its initial efficiency after 3000 h without any encapsulation. Furthermore, 3 × 3 cm2 perovskite mini-modules are presented, achieving a champion efficiency of 18.10%. All these results suggest that the PAPT method is promising for promoting the commercial application of large-area PSCs.

Enhanced Spectral Response of ZnO-Nanorod-Array-Based Ultraviolet Photodetectors by Alloying Non-Isovalent Cu-O with CuAlO2 P-Type Layer.

CuAlO2 was synthesized by a hydrothermal method, in which the Cu-O dimers were incorporated by simply altering the ratio of the reactants and the temperature. The incorporation process increases the grain size in CuAlO2, and modulates the work function and binding energies for CuAlO2 due to the partial substitution of Cu+ 3d10 with Cu2+ 3d9 orbitals in the valence band maximum by alloying non-isovalent Cu-O with a CuAlO2 host. Based on the ZnO nanorod arrays (NRs) ultraviolet photodetector, CuAlO2/Cu-O fabricated by the low-cost drop-coating method was used as the p-type hole transport layer. The incorporation of the Cu-O clusters into CuAlO2 lattice to enhance the conductivity of CuAlO2 is an effective way for improving ZnO NRs/CuAlO2 device performance. The photodetectors exhibit significant diode behavior, with a rectification ratio approaching 30 at ±1 V, and a dark saturation current density 0.81 mA cm-2. The responsivity of the ZnO-NRs-based UV photodetector increases from 13.2 to 91.3 mA/W at 0 V bias, with an increase in the detectivity from 2.35 × 1010 to 1.71 × 1011 Jones. Furthermore, the ZnO NRs/[CuAlO2/Cu-O] photodetector exhibits a maximum responsivity of 5002 mA/W at 1.5 V bias under 375 nm UV illumination.

Design, synthesis, and biological screening of a series of pyrazolo [1,5-a]quina-zoline derivatives as SIRT6 activators.

SIRT6 has emerged as a novel therapeutic target for a variety of diseases. In this study, a total of 102 pyrazolo [1,5-a]quinazoline derivatives were designed and synthesized. The result revealed that 2-methyl-N-(4-phenoxy-phenyl)pyrazolo [1,5-a]quinazoline-5-amine (21q) was the most active compound by structure-activity relationship study, which significantly enhanced SIRT6 defatty-acylation activity with an EC1.5 value of 1.85±0.41 µM and EC50 value of 11.15±0.33 µM. The biological activity of 21q was further verified by differential scanning fluorimetry assay (DSF) and surface plasmon resonance assay (SPR). Molecular docking showed that the pyrazolo [1,5-a]quinazoline of 21q formed a hydrogen bond with Val115 and four π- π interactions with Phe64, Phe82 and Phe86. 21q can significantly improve the thermal stability of SIRT6 protein and inhibit the PI3K/Akt signaling pathway in mouse embryonic fibroblasts (MEFs), thereby inhibiting the proliferation of MEFs. Collectively, we discovered a new potent SIRT6 activator, which can be taken as a lead compound for later studies.

Polymerization-Induced Aggregation Approach toward Uniform Pd Nanoparticle-Decorated Mesoporous SiO2/WO3 Microspheres for Hydrogen Sensing.

Hydrogen as an important clean energy source with a high energy density has attracted extensive attention in fuel cell vehicles and industrial production. However, considering its flammable and explosive property, gas sensors are desperately desired to efficiently monitor H2 concentration in practical applications. Herein, a facile polymerization-induced aggregation strategy was proposed to synthesize uniform Si-doped mesoporous WO3 (Si-mWO3) microspheres with tunable sizes. The polymerization of the melamine-formaldehyde resin prepolymer (MF prepolymer) in the presence of silicotungstic acid hydrate (abbreviated as H4SiW) leads to uniform MF/H4SiW hybrid microspheres, which can be converted into Si-mWO3 microspheres through a simple thermal decomposition treatment process. In addition, benefiting from the pore confinement effect, monodispersed Pd-decorated Si-mWO3 microspheres (Pd/Si-mWO3) were subsequently synthesized and applied as sensitive materials for the sensing and detection of hydrogen. Owing to the oxygen spillover effect of Pd nanoparticles, Pd/Si-mWO3 enables adsorption of more oxygen anions than pure mWO3. These Pd nanoparticles dispersed on the surface of Si-mWO3 accelerated the dissociation of hydrogen and promoted charge transfer between Pd nanoparticles and WO3 crystal particles, which enhanced the sensing sensitivity toward H2. As a result, the gas sensor based on Pd/Si-mWO3 microspheres exhibited excellent selectivity and sensitivity (Rair/Rgas = 33.5) to 50 ppm H2 at a relatively low operating temperature (210 °C), which was 30 times higher than that of the pure Si-mWO3 sensor. To develop intelligent sensors, a portable sensor module based on Pd/Si-mWO3 in combination with wireless Bluetooth connection was designed, which achieved real-time monitoring of H2 concentration, opening up the possibility for use as intelligent H2 sensors.

Corrigendum: A single vaccination of nucleoside-modified Rabies mRNA vaccine induces prolonged highly protective immune responses in mice.

[This corrects the article DOI: 10.3389/fimmu.2022.1099991.].

Effects of mesylate-/tartrate-based ionic liquids-water mixtures on the phase transition behaviors and stability of corn starch: A comparative study.

As one of the most important biopolymers, starch has been applied to replace petroleum-derived polymers for "green" materials. Discovery of novel solvents and understanding of the solvent effects are critical challenges for the destruction of strong hydrogen bonds of starch molecules for manufacturing bio-based materials. Herein, two ionic liquids (ILs), 1-ethyl-3-methyl-imidazolium mesylate ([Emim][MS]) and 1-ethyl-3-methyl-imidazolium tartrate ([Emim][Tar]), were explored as novel solvents for starch. Their effects on phase transition behaviors, microstructure, hydrogen-bond interaction, crystalline structure, micromorphology and thermal stability of corn starch were compared systematically. With the IL/H2O ratio increasing, the starch/IL/H2O mixtures underwent endothermic, exothermic/endothermic and exothermic processes, sequentially. However, the starch properties were very different in two ILs-water systems, which were closely related to the solvent composition and IL structure. These differences were further explained by the interactions among starch, water and the two ILs on the basis of the quantum chemical calculations. It was found that [Emim][MS] had a stronger interaction with water than starch, whereas [Emim][Tar] preferred to bind with starch. This study not only provided experimental supports for understanding the starch behaviors in novel "green" solvents, but also laid the theoretical foundation for starch modification and industrial applications of starch-based materials in more appropriate solvents.

Piezoresistive Fibers with Large Working Factors for Strain Sensing Applications.

Piezoresistive fibers with large working factors remain of great interest for strain sensing applications involving large strains, yet difficult to achieve. Here, we produced strain-sensitive fibers with large working factors by dip-coating nanocomposite piezoresistive inks on surface-modified polyether block amide (PEBA) fibers. Surface modification of neat PEBA fibers was carried out with polydopamine (PDA) while nanocomposite conductive inks consisted of styrene-ethylene-butylene-styrene (SEBS) elastomer and carbon black (CB). As such, the deposition of piezoresistive coatings was enabled through nonconventional hydrogen-bonding interactions. The resultant fibers demonstrated well-defined piezoresistive linear relationships, which increased with CB filler loading in SEBS. In addition, gauge factors decreased with increasing CB mass fractions from ∼15 to ∼7. Furthermore, we used the fatigue theory to predict the endurance limit (Ce) of our fibers toward resistance signal stability. Such a piezoresistive performance allowed us to explore the application of our fibers as strain sensors for monitoring the movement of finger joints.

3D printed bionic self-powered sensing device based on fern-shaped nitrogen doped BiVO4 photoanode with enriched oxygen vacancies.

A portable three-dimensional (3D) printed bionic sensing device with enhanced photoelectric response was fabricated for sensitive detection of Bisphenol A (BPA). The proposed sensor is operated upon by using a highly reactive dual-electrode system to generate electrical output and provide the sensing signal under photoirradiation, without an external power source. The fern-shaped nitrogen doped BiVO4 photoanode with enriched oxygen vacancies (Ov) bismuth vanadate (N/Ov/BiVO4) photoanode was first synthesized and applied to construct a bionic sensing device. Density functional theoretical (DFT) calculation shows that the synergistic of nitrogen doping and Ov on the surface of photoanode leads to the emergence of impurity levels in BiVO4's electronic structure, promoting the effective separation of photogenerated electron-hole pairs. Impressively, the unique fern-shaped bionic structure enhances the mass transfer efficiency of the sensing system and provides abundant binding sites of aptamer, realizing signal amplification. Moreover, a portable sensing device for automatic sample injection and detection is developed by integrating the detection system into a micromodel based on micro-nano 3D printing technology. Benefit from this ingenious design, the proposed bionic aptasensor displayed excellent electricity output and achieved high sensitivity and selectivity of BPA detection with a low limit of detection (0.025 nM) and broad linear range from 0.1 nM to 100 µM, paving a new way for the development of portable and on-site sensing devices.

Change of tourism organizations: Implications from a review of cultural tourism research.

Change has been universally acknowledged as the perpetual theme for routine organizational life. As cultural tourism, a major element of global tourism consumption accounting for 40% of tourism employment, is becoming increasingly flourishing and promising, tourism organizations are also obliged to implement a series of organizational changes to adapt to the trending culturalization in the tourism domain. In light of this, this research, by outlining important sub-themes and trends of cultural tourism research, tracks the evolution of cultural tourism as a research field over the previous decades so as to analyze existing interconnections between the systematic review and tourism organizational change. Based on these interconnections, the research also manages to propose several potential implications for tourism organizations to optimize their future implement of daily organizational changes for the sake of adaptative survival and development.

Electromechanical Performance of Strain Sensors Based on Viscoelastic Conductive Composite Polymer Fibers.

Flexible conductive polymer composite (CPC) fibers that show large changes in resistance with deformation have recently gained much attention as strain-sensing components for future wearable electronics. However, the electrical resistance of these materials decays with time during dynamic cyclic loading, a deformation performed to simulate their real application as strain sensors. Despite the extensive research on CPC fibers, the mechanism leading to this decay in the electromechanical response under repetitive cycles remains unreported. Herein, this behavior is investigated using fiber-based strain sensors wet spun from thermoplastic polyurethane (TPU) consisting of a carbonaceous hybrid conductive filler system of carbon black (CB) and carbon nanotubes (CNTs). We found electrical viscosity to predict the observed electromechanical resistance decay. This implies that cycling these materials enables the relaxation of both the polymer chains and the conductive network. In addition, the resulting piezoresistive fibers are sensitive to deformation in the region of low strain (gauge factor of 6.0 within 3.0% strain), remain conductive under 280.5% deformation, and are stable for more than 2000 cycles. Finally, we demonstrate the potential of TPU/CB-CNT fibers as strain sensors for monitoring human motion.

Research on Translation of Chinese Medicine Constitution (Tizhi) Academic Terms: Based on Memetics and Delphi Method.

Background: With the continuous in-depth research of Chinese medicine constitution (tizhi) and the continuous expansion of cross research with new disciplines, internationalization will become the future trend of Chinese medicine constitution (tizhi). Translating the terms of Chinese medicine constitution (tizhi) into English is the first step for Chinese medicine constitution (tizhi) to go international. Language memes play an important role in information transmission in social interpersonal communication activities. The continuous replication and dissemination of translation memes make language spread and popularized. Because there is no fixed translation method at present, based on the particularity of Chinese medicine constitution (tizhi), we decided to use the Delphi method to complete the term translation research. Objective: The purpose of this study is to provide a standard and unified translation method for terms of traditional Chinese medicine (TCM) constitution with Chinese characteristics through the Delphi expert consultation strategy. Methods: Forward translation and expert consensus were conducted to complete this study. We sorted out the related terms of Chinese medicine constitution (tizhi) theory and invited an expert from the World Federation of Chinese Medicine Societies (WFCMS) to complete the initial forward translation. An expert of Chinese medicine constitution (tizhi) theory joined this process. Then, we invite relevant professionals to evaluate this translation version using the Delphi method. Results: Following a 3-round Delphi survey, the translation criteria of 61 (92.42%) terms were unified, and 5 terms resulted in no consensus and reached consensus on the translation method of Chinese medicine constitution (tizhi) theory. A major problem about how to translate "" is identified. 25 experts participated in this study, and the drop-out rate is 0% in the 3-round Delphi survey. Translation challenges include the following: (1) translation methods of "Chinese medicine constitution (tizhi) theory"; (2) experts' understanding deviation on the definitions of some terms. Conclusions: The average mode, full score ratio, standard deviation, coefficient of variation, and variation ratio of expert scores are analyzed. The diversity of regions and professional titles of experts shows that they have a high degree of authority. The scores of terms indicate the consistent of study results, so they can be used as a reference for the translation of Chinese medicine constitution (tizhi) theory.

Obeticholic acid aggravates liver injury by up-regulating the liver expression of osteopontin in obstructive cholestasis.

AIMS: Obeticholic acid (OCA) was approved for the treatment of primary biliary cholangitis (PBC) patients, as it can significantly improve the level of serum alkaline phosphatase. However, OCA-induced liver injury in PBC patients puts them at risk of acute chronic liver failure, thus limiting the clinical application of OCA. Osteopontin (OPN), an extracellular cell matrix molecule, is highly induced in many cholestatic liver diseases. Herein we explored whether liver injury exacerbation by OCA was related to OPN. MAIN METHODS: Bile duct ligation (BDL) mice were treated with OCA (40 mg/kg) to evaluate its effect on liver injury and OPN involvement. Enzyme-linked immunosorbent assay, western blot, immunohistochemistry, and other assays were used to detect OPN levels in serum and liver. Immunohistochemistry, and immunofluorescence, among other assays, were used to evaluate the extent of ductular reaction. The extent of fibrosis was also determined using various assays, such as immunohistochemistry, quantitative real-time PCR (qPCR), and hydroxyproline assays. KEY FINDINGS: OPN was overexpressed in the liver of BDL mice treated with OCA. OCA induced overexpression of OPN exacerbated ductular reaction, fibrosis, and liver inflammation, and reduced hepatocyte proliferation. SIGNIFICANCE: Upon liver injury, OCA upregulates the expression of OPN in the liver and accelerates disease progression. This mechanism helps explain the risk of liver damage associated with OCA.

Uncertainty of Artificial Intelligence Assistant: The Effect of Assistant Type on Variety Seeking.

In service marketing, AI assistants and self-service technology have become popular. As a result, it is critical to enrich the understanding of whether consumers react differently in the artificial intelligence (AI) service context in comparison with the human service context. This study examines the effect of assistant type (AI vs. human) on consumers' decision-making. Through three experiments, this research finds that variety seeking will be higher when consumers are making decision in AI (vs. human) service environment. Furthermore, we tested uncertainty as the underlying mechanism. Moreover, we demonstrated that this pattern is moderated by situational involvement. Specifically, in consumption contexts of high involvement, the consumers are less likely to seek variety, and in consumption contexts of low involvement, they prefer more variety (study 3). This research offers service providers new insights by revealing how, why, and when the interaction of AI technology influences consumers' decision-making in service marketing.

A flexible photoelectrochemical aptasensor using heterojunction architecture of α-Fe2O3/d-C3N4 for ultrasensitive detection of penbritin.

The performance of photoelectrochemical (PEC) analysis system relies closely on the properties of the photoelectric electrodes. It is of great significance to integrate photoactive materials with flexible substrates to construct ultra-sensitive PEC sensors for practical application. This work reports a novel photoelectrode developed by immobilizing α-Fe2O3 nanoparticles (NPs)/defect-rich carbon nitride (d-C3N4), an excellent Z-scheme heterojunction photoelectric material, onto three-dimensional (3D) flexible carbon fiber textile. Specifically, 3D hierarchical structure of flexible carbon fiber textile provides larger specific surface area and higher mechanical strength than traditional electrodes, resulting in more reaction sites and faster reaction kinetics to achieve signal amplification. Simultaneously, α-Fe2O3/d-C3N4 Z-scheme heterojunction exhibits enhanced light absorption capability and high redox ability, thus dramatically improving the PEC performance. This photoelectrode was used to construct a flexible PEC aptasensor for ultrasensitive detection of penbritin, demonstrating excellent performance in terms of wide linear range (0.5 pM-50 nM), low detection limit (0.0125 pM) and high stability. The design principle is applicable to the manufacture of other photoelectric sensing systems, which provides an avenue for the development of portable environmental analysis and field diagnostics equipment.

A single vaccination of nucleoside-modified Rabies mRNA vaccine induces prolonged highly protective immune responses in mice.

Background: Rabies is a lethal zoonotic disease that kills approximately 60,000 people each year. Although inactivated rabies vaccines are available, multiple-dose regimensare recommended for pre-exposure prophylaxis or post-exposure prophylaxis,which cuts down the cost- and time-effectiveness, especially in low- and middle incomecountries. Methods: We developed a nucleoside-modified Rabies mRNA-lipid nanoparticle vaccine (RABV-G mRNA-LNP) encoding codon-optimized viral glycoprotein and assessed the immunogenicity and protective efficacy of this vaccine in mice comparing to a commercially available inactivated vaccine. Results: We first showed that, when evaluated in mice, a single vaccination of RABV-G mRNA with a moderate or high dose induces more potent humoral and T-cell immune responses than that elicited by three inoculations of the inactivated vaccine. Importantly, mice receiving a single immunization of RABV-G mRNA, even at low doses, showed full protection against the lethal rabies challenge. We further demonstrated that the humoral immune response induced by single RABV-G mRNA vaccination in mice could last for at least 25 weeks, while a two-dose strategy could extend the duration of the highly protective response to one year or even longer. In contrast, the three-dose regimen of inactivated vaccine failed to do so. Conclusion: Our study confirmed that it is worth developing a single-dose nucleoside-modified Rabies mRNA-LNP vaccine, which could confer much prolonged and more effective protection.