Retraction of "Cu(II)-Containing Metal-Organic Framework with 1D Hexagonal Channels for Cyanosilylation Reaction and Anticancer Activity on Osteosarcoma Cells".

[This retracts the article DOI: 10.1021/acsomega.0c06270.].

miR-9-5p Mediates ABCC1 to Elevate the Sensitivity of Glioma Cells to Temozolomide.

Chemotherapy combined with surgery is an important clinical treatment for glioma, but endogenous or acquired temozolomide (TMZ) resistance can lead to poor prognosis. microRNA (miR)-9-5p acts in biological function of glioma, but the drug resistance of miR-9-5p in glioma is under exploration. The study intended to test the molecular mechanism of miR-9-5p in glioma cells. MTT assay was applied to investigate the chemosensitivity enhancement of miR-9-5p on TMZ in glioma cells U87-TMZ and U251-TMZ, and in vivo experiments confirmed its role on tumor growth in nude mice. The results of double luciferase reporter gene assay, qRT-PCR and WB indicated that miR-9-5p directly targeted ABCC1 (ATP binding cassette subfamily C member 1) to reduce its expressions. MTT and flow cytometry indicated that elevation of miR-9-5p or down-regulation of ABCC1 could inhibit proliferation-induced apoptosis of drug-resistant cells, and the decrease of miR-9-5p could reverse the reduction of ABCC1 on proliferation-induced apoptosis of drug-resistant cells. In vivo experiments showed that miR-9-5p could promote the anti-tumor role of TMZ. To sum up, the increase of miR-9-5p directly targets ABCC1 and may make glioma cells sensitive to TMZ.

Cu(II)-Containing Metal-Organic Framework with 1D Hexagonal Channels for Cyanosilylation Reaction and Anticancer Activity on Osteosarcoma Cells.

A novel metal-organic framework (MOF) has been produced via Cu(NO3)2·6H2O reaction with 3-(1H-tetrazol-5-yl)pyridine (HL) in water, and its chemical formula is {[(Cu(L)2(H2O)2](H2O)8) n } (1). Due to its high density of coordinately unsaturated sites along with large one-dimensional (1D) hexagonal channels, the activated complex 1 (1a) was explored as the solvent-free heterogeneous catalyst for cyanosilylation under mind conditions. The inhibitory function of compound 1a against the survival rate of OS-732 osteosarcoma cells was evaluated via Cell Counting Kit-8 (CCK-8) detection kit. Furthermore, the Annexin V-FITC/PI detection kit and the active oxygen (ROS) detection kit was carried out to determine the cell apoptosis levels and the ROS accumulation in OS-732 osteosarcoma cells after treatment by compound 1a.

Invasiveness-triggered state transition in malignant melanoma cells.

Cancer cells are considered to have high morphological heterogeneity in human melanoma tissue. Here, we report that epithelial cancer cells are dominant in different development stages of human melanoma tissues. The cellular and molecular mechanisms that maintain melanoma cells in the epithelial state are further investigated in the A2058 cell line. We find that micropore (8 µm) transwell invasion, but not superficial migration in the scratch assay, can induce remarkable morphological changes between epithelial and mesenchymal melanoma cells within 4 days. The morphological switch is associated with dynamic changes of epithelial-mesenchymal transition (EMT) hallmarks E-cadherin and vimentin. Further immunoflurencent staining and co-immunoprecipitation assay showed the uncoupling of the M3 muscarinic acetylcholine receptor (mAChR) and the p75 neurotrophin receptor (p75NTR) in epithelial melanoma cells. Specific knockdown of M3 mAChR by small interfering RNA (siRNA) significantly abrogates the transition of spindle-shaped mesenchymal cells to epithelial cells. Collectively, we report a cellular model of invasiveness-triggered state transition (ITST) in which melanoma cell invasion can induce morphological changes between epithelial and mesenchymal cells. ITST is one of the biological basis for maintaining metastatic melanoma cells in the epithelial state. Furthermore, M3 mAChR receptor-mediated ITST provides a novel therapeutic strategy to inhibit the development of malignant melanoma.

Clinical features and prognostic factors in children with fulminant myocarditis / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To investigate the clinical features and prognostic factors in children with fulminant myocarditis.</p><p><b>METHODS</b>The clinical data of 24 children with fulminant myocarditis were retrospectively analyzed. According to the prognosis, these children were classified into two groups: survival (n=12) and death (n=12). The risk factors influencing prognosis in children with fulminant myocarditis were identified by logistic regression analysis.</p><p><b>RESULTS</b>Among the 24 cases of fulminant myocarditis, gastrointestinal symptoms were found as initial symptoms in 14 cases, neurological symptoms in 12 cases, respiratory symptoms in 1 case, and cardiac symptoms in 2 cases. On admission, serum levels of creatine kinase MB, troponin I, and brain natriuretic peptide (BNP) were all increased. Besides, left ventricular ejection fraction (LVEF) decreased in 22 cases (92%), cardiothoracic ratio increased in 10 cases, third-degree atrioventricular block was observed in 8 cases, ST-segment changes were found in 11 cases and ventricular tachycardia was identified in 2 cases. LVEF in the death group was lower than in the survival group (P<0.05), while the peak level of serum BNP during hospitalization in the death group was higher than in the survival group (P<0.05). The multivariate logistic regression analysis revealed that LVEF was the risk factor influencing prognosis (OR=7.418; P<0.05).</p><p><b>CONCLUSIONS</b>Fulminant myocarditis has no specific clinical features in children. A decreased LVEF is a risk factor for poor prognosis in children with fulminant myocarditis.</p>

Short-term prognostic factors in children with acute liver failure / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To investigate the factors that influence the short-term (6 months) prognosis in children with acute liver failure.</p><p><b>METHODS</b>The clinical information of 53 children with acute liver failure treated between June 2008 and September 2013 was retrospectively analyzed. The patients were divided into survival group (n=21) and death group (n=32) according to their outcomes. The liver function parameters and incidence of complications were compared between the two groups, and multivariate logistic regression analysis was used to identify major factors affecting the short-term prognosis in these patients.</p><p><b>RESULTS</b>There were significant differences between the death and survival groups in the indices of international normalized ratio (INR), blood ammonia and serum albumin (Alb), and complications such as hepatic encephalopathy, gastrointestinal hemorrhage, and multiple organ failure (P<0.05). Multivariate logistic regression analysis demonstrated that serum Alb, INR, and hepatic encephalopathy were the major factors affecting the short-term prognosis of acute liver failure (OR=0.616, 75.493 and 1210.727 respectively; P<0.05).</p><p><b>CONCLUSIONS</b>INR, hepatic encephalopathy and serum Alb are the major factors that influence the short-term prognosis in children with acute liver failure.</p>

[Effects of adsorbents on partitioning of Cd and Pb in MSW incineration].

An incineration experiment using simulated municipal solid waste (MSW) in the tubular furnace was performed to study the effects of SiO2, Al2o3 and CaO on the partitioning of Cd and Pb under different operating conditions which included adsorbents percentage, furnace temperature and residence time. The results indicated that the increasing of SiO2, AL2O3, CaO concentration were favorable for Cd to partition in the bottom ash. And for Pb, SiO2 and Al2O3 had the same effects. However, the condition with CaO showed an opposite tendency of increasing Pb distribution in fly ash. While all of these adsorbents increasing, the effects were also enhanced. When the temperature of the tubular furnace was 850 degrees C, the adsorption efficiencies of these adsorbents for Cd were in the sequence of CaO > Al, O3 > SiO2, and for Pb, Al2O3 > SiO2 > CaO. In addition,with increasing of tubular furnace temperature and residence time, there would be more Cd and Pb compounds moving to the fly ash.

Negative regulation of endogenous protein kinase Calpha on the dynamic change of carbachol-induced intracellular calcium response in different melanoma cells.

Regulations of intracellular protein kinase C (PKC) on carbachol (CCh)-induced intracellular calcium ([Ca(2+)]i) responses were investigated in different stages of melanoma cells. We found that CCh (1 mM) significantly increased [Ca(2+)]i with 6-, 4-, 4-, and 25-folds intensities in WM793B, 451Lu, SK-MEL-5, and A2058 melanoma cells, respectively. Pretreatment of phorbol 12, 13-dibutyrate (PDBu, 2 microM), an activator of intracellular PKC, significantly suppressed CCh-induced peak reactions in WM793B, SK-MEL-5, and A2058 cells. RT-PCR data showed that mRNA levels of PKCalpha were 12-, 4-, 6-, and 0.9-folds higher in above four melanoma cells. Short interfering RNA (siRNA) targeting to PKCalpha in WM793B cells enhanced CCh-induced peak calcium reactions. Present data indicated that CCh-induced [Ca(2+)]i responses were dynamically changed in different stages of melanoma progression. Moreover, intracellular PKCalpha activated by exogenous agonist and expressed through endogenous gene transcription negatively regulated CCh-induced calcium responses. The functional analysis on the relationship between CCh-induced calcium response and endogenous PKCalpha expression might be helpful to predict the development of melanoma.

[Effects of sulfur compounds on Pb partitioning in a simulated MSW incinerator].

The effect of sulfur compounds (including sulfur, sulfide, sulfite and sulfate), initial concentration of heavy metal and operating conditions on Pb emission in MSW incineration were investigated using a simulated tubular furnace with the simulated MSW. Operating conditions of the experiment included combustion chamber temperature and MSW residence time. The concentration of Pb was measured by ICP-AES after the digesting of samples including bottom ash, fly ash and flue gas according to related USEPA methods. The results indicated that all 4 sulfur compounds tended to increase Pb partitioning in fly ash and decrease Pb partitioning in bottom ash. The increasing of S and Na2S content tended to decrease Pb partitioning in bottom ash, meanwhile, the content of Na2SO3 and Na2SO4 have no significant effects on Pb partitioning. Incineration temperature showed a significant effect on Pb volatilization, and thus the Pb partitioning in fly ash increasing along with temperature went upwards. Pb did not partition in flue gas during the whole experimental temperature range. Furthermore, the effect of initial concentration of heavy metal had a significant influence on Pb partitioning. The more initial concentration of Pb was, the more Pb partitioned in bottom ash. MSW residence time was also investigated. The longer MSW residence time was, the less Pb partitioned in bottom ash.

[Effects of chlorides on Cd partitioning and speciation in a simulated MSW incinerator].

The effects of chlorides (including inorganic chloride NaCl and organic chloride PVC) on Cd emission in MSW (municipal solid waste) incineration was investigated using a simulated tubular furnace and the simulated MSW spiked with heavy metals. The concentrations of heavy metals were measured by ICP-AES after the digesting of samples including bottom ash, fly ash and flue gas according to related USEPA Methods. Heavy metal species in bottom ash and fly ash were identified by X-ray diffraction technique and the spectra of elements distributed and micrographs of bottom ash and fly ash were detected by energy dispersive X-ray system and scanning electron microscope, respectively. The results indicated that the emission of Cd tended to be enhanced with the increasing of chloride content, and 97% Cd partitioned in fly ash after spiked chlorides. However, the content effect of inorganic chloride NaCl and inorganic chloride PVC on Cd emission had no significant difference. The effect of PVC on Cd partitioning was affected by temperature, 80.51% Cd partitioned in fly ash at 550 degrees C and 97.91% Cd partitioned in fly ash at 850 degrees C, when temperature went above the melting point of CdCl2 (568 degrees C), the effect of temperature became less important. The effect of NaCl on Cd partitioning was not affected by temperature, 95.02% Cd partitioned in fly ash at 550 degrees C and 96.58% Cd partitioned at 1 000 degrees C when spiked NaCl. Residence time had no significant effect on Cd partitioning with spiked chlorides. The two different mechanisms of chlorides effects on Cd emission were identified by bottom ash and fly ash analysis using SEM/EDS and XRD technique.

[Gene cloning and expression analysis of a male sterility related gene BoDHAR from broccoli].

A differentially expressed cDNA fragment obtained from a cDNA-AFLP analysis, which performed on floral buds of male sterile and fertile lines of cabbage, was used as a querying probe to blast the Genbank and Arabidopsis databases. Based on the assembled homologous cDNA sequences, a full-length cDNA of 633 bp for BoDHAR was cloned by RT-PCR. Furthermore, we have experimentally cloned and sequenced the 5' flanking sequence of gene BoDHAR by genomic walking method based on ligation-mediated PCR. The full length DNA sequence with 1486bp, containing two introns, was achieved. Homologous analysis shows that gene has 82.3% identity at nucleotide level, and 79.6% identity at amino acid level with Arabidopsis dehydroascorbate reductase (DHAR) gene AT1 G19570.1. Structurally, BoDHAR encodes a polypeptide of 210 amino acids, which contains a GST-c-DHAR domain highly conserved among other members of the DHAR superfamily and has multiple phosphorylation sites. Promoter predictions software indicated that the 5' upstream region contained putative transcription signals and conserved sequences, one CAAT-box, one G-box and four TGAC-like motifs. To advance our understanding of gene BoDHAR, tissue expression pattern were analyzed by semi-quantitative RT-PCR. The results indicate that expression level of gene BoDHAR is higher in fertile buds than that in sterile buds, and expressed intensively in the anther.

[Induction of IgG and IgM production by Epstein-Barr virus protein].

AIM: To detect the production of IgG and IgM by cultured umbilical blood B cell stimulated by UV and heat-inactivated Epstein-Barr virus (EBV). METHODS: Mononuclear cells were isolated routinely from umbilical blood, then monocytes, NK cells and cytotoxic T cells were eliminated by L-leucine methy ester method; remained T cells were eliminated by AET-SRBC rosette method. The purified B cells were treated with UV or heat-inactivated EBV respectively and cultured in complete IMDM. The IgG and IgM in the supernatant were detected by ELISA. RESULTS: The IgG and IgM production(A value) in UV-inactivated EBV group increased from 18th day to 30 th day after stimulation compared with the baseline. The A value of all time points in heat-inactivated EBV group had no significant difference. CONCLUSION: UV-inactivated EBV may induce IgG and IgM production with a time-effect relationship, suggesting that EBV protein is a key component of stimulating Ig production. This result provide the basis for the further researching the effects of functional EBV protein on the production of natural autoantibodies (NAA).