Rituximab combined with Bruton tyrosine kinase inhibitor to treat elderly diffuse large B-cell lymphoma patients: Two case reports.

BACKGROUND: Diffuse large B-cell lymphoma (DLBCL) is a common aggressive non-Hodgkin's lymphoma (NHL), accounting for 30%-40% of adult NHLs. This report aims to explore the efficacy and safety of rituximab combined with Bruton tyrosine kinase inhibitors (BTKis) in the treatment of elderly patients with DLBCL. CASE SUMMARY: The clinical data of two elderly patients with DLBCL who received rituximab combined with BTKi in our hospital were retrospectively analyzed, and the literature was reviewed. The patients were treated with chemotherapy using the R-miniCHOP regimen for two courses. Then, they received rituximab in combination with BTKi. CONCLUSION: The treatment experience in these cases demonstrates the potential efficacy of rituximab combined with BTKi to treat elderly DLBCL patients, thus providing a new treatment strategy.

Effects of Danshen Injection () on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells.

OBJECTIVE: To explore the effects of Danshen Injection () on inhibition proliferation, inducing apoptosis and its possible mechanisms on human erythroid leukemic (HEL) cells. METHODS: The commercial Chinese patent medicine of Danshen Injection was extracted and isolated from Chinese herb of Salvia miltiorrhiza bung. The inhibition effects of proliferation were assayed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method in HEL cells treated by Danshen Injection at various concentrations for 48 h. The cellular apoptosis was observed in morphology, analyzed by flow cytometry with annexin V and propidium iodide (PI) staining, and examined by DNA degradation ladder on agarose gel electrophoresis. Meanwhile, the expression levels of mutant Janus kinasez (JAK2) gene and phosphorylation-JAK2 (P-JAK2) protein were detected by allele specific-polymerase chain reaction and Western blot. RESULTS: The proliferation of HEL cells was effectively inhibited by Danshen Injection in a dose-dependent manner, with suppression rates from 19.46±2.31% to 50.20±5.21%. Typical apoptosis cells was observed in Danshen Injection treated HEL cells, the rates of annexin V positive cells increased obviously in a dose-dependent manner, as well as the DNA degradation ladder of apoptosis revealed on gel electrophoresis. The expression levels of mutant JAK2 gene and P-JAK2 protein reduced gradually with increasing dosage of Danshen injection. CONCLUSION: Danshen Injection could not only significantly inhibit the proliferation, but also induce apoptosis in HEL cells; down-regulation of the mutant JAK2 gene and P-JAK2 protein expressions are probably one of its molecular mechanisms.

The effect of polymorphism F279Y of GHR gene on milk production trait in Chinese Holstein cattle / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To study the effect of the polymorphism F279Y of the growth hormone receptor (GHR) gene on milk yield and composition in Chinese Holstein cattle.</p><p><b>METHODS</b>Hundred thirty two Chinese Holstein cattle were selected as study materials, according to DHI production performance method to get the data of milk yield and composition; PCR- SSCP and sequencing method were used to detect the genotypes; least square method was used to acquire correlation analysis.</p><p><b>RESULTS</b>Chinese Holstein cattle F279Y of GHR gene loci A and T allele frequency were 0.68 and 0.32, respectively, the experimental group significantly deviated from Hardy Weinberg equilibrium (P < 0.01); 305 d milk yield of AA genotype was significantly higher than AT type (P < 0.05), 305 d milk fat yield, 305 d milk protein yield and 305 d lactose of AT type had better trend than those of AA type in numeric; Therefore, allele A was dominant gene of high milk yield, allele T has positive effect on milk composition.</p><p><b>CONCLUSION</b>Mutation F279Y of GHR gene can be used as genetic markers in Chinese Holstein milk production traits of marker assisted selection (MAS) breeding.</p>