RESUMO
OBJECTIVE: To study the coumarin glucosides of Angelica dahurica. METHODS: Fresh roots and rhizomes of Angelica dahurica were extracted with ethanol at room temperature. Repeated column chromatography and preparative HPLC were used to isolate and purify the compounds. Their structures were elucidated on the basis of chemical evidence and spectral analysis. RESULTS: Twenty-six coumarin glucosides were isolated from Angelica dahurica, while here we reported 7 of them: sec.-O-beta-D-Galactopyranosyl-(R)-byakangelicin (I);8-O-beta-D-Galactopyranosyl-xanthotoxol (II); 7-O-beta-D-Apiofuranosyl-(1-->6)-beta-D-Glucopyranosyl-peucedanol (III); (R)-peucedanol-7-O-beta-D-Glucopyranoside (IV); sec.-O-beta-D-Glucopyranosyl-(R)-Oxypeucedaninhydrate (V); 7-O-beta-D-Galactopyranosyl-Sco-poletin( VI); Aesculin (VII). CONCLUSION: Compound V is a new compound, Compound VII is isolated from Umbellifera for the first time, compounds III, IV are isolated from Angelica for the first time,while compound I, II and VI are isolated from this plant for the first time.
Assuntos
Angelica/química , Cumarínicos/química , Glucosídeos/química , Raízes de Plantas/química , China , Cumarínicos/isolamento & purificação , Etanol/química , Furocumarinas/química , Furocumarinas/isolamento & purificação , Glucosídeos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Rizoma/químicaRESUMO
OBJECTIVE: To research the chemical constituents from stems and leaves of Lonicera macranthoides. METHODS: Various column chromatographies were employed to isolate and purify the constituents. Their structures were elucidated by spectral analysis (IR, MS, 1H-NMR, 13 C-NMR) and chemical evidence. RESULTS: Nine constituents were obtained and identified as loganin (I), loganic acid (II), morroniside (III),7-O-ethyl-morroniside (IV), scopoletin (V), caffeic acid (VI), chlorogenic acid (VII), beta-sitosterol (VIII), daucosterol (IX). CONCLUSION: Compounds I-VI are isolated from the plant for the first time. All the compounds are found for the first time from the stems and leaves of Lonicera macranthoides.
Assuntos
Glicosídeos/isolamento & purificação , Iridoides/isolamento & purificação , Lonicera/química , Plantas Medicinais/química , Escopoletina/isolamento & purificação , Ácidos Cafeicos/química , Ácidos Cafeicos/isolamento & purificação , Glicosídeos/química , Iridoides/química , Estrutura Molecular , Folhas de Planta/química , Caules de Planta/química , Escopoletina/químicaRESUMO
OBJECTIVE: To study the chemical constituents from the bulbs of Zephyranthes candida. METHODS: The chemical constituents were separated and purified by means of several chromatographies and their structures were elucidated by spectroscopic methods. RESULTS: Six compounds were isolated and identified as trisphaeridine (I), p-hydroxybenzaldehyde (II), sucrose (III), (-)-amarbellisine (IV), lycorine (V), (+)-haemanthamine (VI). CONCLUSION: Compounds I - IV are obtained from this genus for the first time.
Assuntos
Benzaldeídos/isolamento & purificação , Dioxóis/isolamento & purificação , Liliaceae/química , Fenantridinas/isolamento & purificação , Alcaloides de Amaryllidaceae/química , Alcaloides de Amaryllidaceae/isolamento & purificação , Benzaldeídos/química , Dioxóis/química , Etanol/química , Espectroscopia de Ressonância Magnética , Fenantridinas/química , Raízes de Plantas/química , Sacarose/química , Sacarose/isolamento & purificaçãoRESUMO
Macranthoside B (MB) is a hederagenin saponin extracted from the flower bud of Lonicera macranthoides. In this study, we defined the anticancer effect of MB both in vitro and in vivo using cell proliferation assays and xenograft tumor growth assays. Our data indicate that MB inhibits the proliferation of various kinds of cancer cells with IC(50) values in the range of 10-20 microM. Moreover, the volume and weight of xenograft tumors in nude mice treated with 5mg/kgMB were decreased remarkably compared to those of the vehicle control group. Furthermore, DAPI staining and flow cytometry analysis with Annexin V/PI double staining revealed that more apoptotic cells were observed following MB treatment. In addition, degradation of PARP (poly-ADP-ribose polymerase), and activation of the caspase cascade for intrinsic pathways were observed. We also found that the expression of Bcl-2 protein decreased and the protein level of Bax increased which leading to an increase of the Bax/Bcl-2 ratio. Our results showed that MB exhibited strong anti-tumor effect and mitochondrion-mediated apoptosis induction involved in it.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Lonicera/química , Ácido Oleanólico/análogos & derivados , Saponinas/farmacologia , Animais , Anexina A5/biossíntese , Anexina A5/genética , Apoptose/efeitos dos fármacos , Western Blotting , Caspases/genética , Caspases/fisiologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Feminino , Citometria de Fluxo , Humanos , Indicadores e Reagentes , Camundongos , Camundongos Endogâmicos BALB C , Mitocôndrias/efeitos dos fármacos , Ácido Oleanólico/farmacologia , Poli(ADP-Ribose) Polimerases/biossíntese , Poli(ADP-Ribose) Polimerases/genética , Proteínas Proto-Oncogênicas c-bcl-2/biossíntese , Proteínas Proto-Oncogênicas c-bcl-2/genética , Transdução de Sinais/efeitos dos fármacos , Ensaios Antitumorais Modelo de Xenoenxerto , Proteína X Associada a bcl-2/biossíntese , Proteína X Associada a bcl-2/genéticaRESUMO
OBJECTIVE: To study the chemical constituents of flower buds of Lonicera macranthoides. METHODS: The 90% EtOH extract of Lonicera macranthoides. was successively partitioned with petroleum ether and ethyl acetete. RESULTS: Repeated column chromatography of the ethyl acetete fraction afforded the following compounds (1-9): ginnol (1), triacontanol (2), ursolic acid (3), beta-sitosterol (4), triacontane (5), palmitic acid (6), beta-daucosterol (7), 3-decyl-3-octyldocosan-1-ol (8), 3-dodecyl-3-nonyldocosan-1-ol (9). All compounds except 4 are isolated from this plant for the first time while compounds 2, 3, 5, 8 and 9 are their first time been isolated from genus Lonicera.
Assuntos
Álcoois Graxos/isolamento & purificação , Lonicera/química , Parafina/isolamento & purificação , Plantas Medicinais/química , Triterpenos/isolamento & purificação , Álcoois Graxos/química , Flores/química , Espectroscopia de Ressonância Magnética , Ácido Palmítico/química , Ácido Palmítico/isolamento & purificação , Parafina/química , Sitosteroides/química , Sitosteroides/isolamento & purificação , Triterpenos/química , Ácido UrsólicoRESUMO
OBJECTIVE: To study the chemical constituents of the roots of Angelica pubescens. METHODS: Fresh roots of Angelica pubescens were extracted with EtOH,the chemical constituents in the extract were isolated by column chromatography as macroporous absorption resin,and ODS etc. Their structures were confirmed by spectral analysis (NMR, MS). RESULTS: Seven compounds were isolated and their structures were identified as nodakenetin (I), umbelliferone (II), columbianetin (III), beta-D-glucosyl-columbianetin (IV), columbianin (V), tert. -O-beta-D- glucopyranosyl -(R)-by-akangelicin (VI) and sec. -O-beta-D- glucopyranosyl-(R) -byakangelicin (VII). CONCLUSION: Compound IV, VI and VII are isolated from Angelica pubescens for the first time.