RESUMO
The practical application of genistein as a low toxicity chemotherapeutic drug is hindered by many of its in vivo properties. To overcome these obstacles, a new multifunctional drug delivery system is developed, which is based on covalently attaching genistein onto Fe3O4 nanoparticles coated by cross-linked carboxymethylated chitosan (CMCH). The structure of the Fe3O4-CMCH-genistein nano-conjugate was confirmed by transmission electron micrographs (TEM), X-ray diffraction (XRD) and Fourier-transfer infrared (FT-IR) spectroscopy. The nano-conjugate shows good water solubility and superparamagnetic properties with a saturation magnetization of 55.1 emu/g. The effects of free genistein and FeO4-CMCH-genistein nano-conjugate on the proliferation and apoptosis of gastric cancer cell line SGC-7901 were investigated by MTT assay and flow cytometry (FACS). MTT results indicate that the Fe3O4-CMCH-genistein nano-conjugate exhibits a significantly enhanced inhibition effect to the SGC-7901 cancer cells than the free genistein. FACS data suggests that the inhibition on cell proliferation of the nano-conjugate is related with an induced apoptosis process. This drug delivery system is promising for future multifunctional chemotherapeutic application that combines drug release and magnetic hyperthermia therapy.
Assuntos
Portadores de Fármacos/química , Compostos Férricos/química , Genisteína/administração & dosagem , Genisteína/química , Nanopartículas/química , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/fisiopatologia , Anticarcinógenos/administração & dosagem , Anticarcinógenos/química , Materiais Biocompatíveis/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Portadores de Fármacos/efeitos da radiação , Campos Eletromagnéticos , Compostos Férricos/efeitos da radiação , Humanos , Magnetismo , Nanopartículas/ultraestrutura , Neoplasias Gástricas/patologiaRESUMO
There are two independent mol-ecules in the asymmetric unit of the title compound, C(37)H(26)N(2)O(2). The crystal structure is stabilized by a weak intra-molecular hydrogen bond as well as C-Hâ¯π inter-actions.
RESUMO
The title compound, C(8)H(5)N(5), was synthesized from phthalonitrile. The benzonitrile and tetra-zole rings are inclined at a dihedral angle of 37.14â (11)°. In the crystal structure, inter-molecular N-Hâ¯N hydrogen bonds link the tetra-zole rings of adjacent mol-ecules, forming chains along the a axis.
RESUMO
The mol-ecule of the title compound, C(14)H(9)N(3), is essentially planar, the dihedral angle formed by the benzimidazole ring system with the benzene ring being 3.87â (3)°. In the crystal packing, mol-ecules are linked into zigzag chains running parallel to the b axis by inter-molecular N-Hâ¯N hydrogen-bond inter-actions.
RESUMO
In the title compound, C(8)H(8)N(4)S, the planar triazole ring forms a dihedral angle of 13.7â (2)° with the phenyl ring. The crystal structure is stabilized by inter-molecular N-Hâ¯S hydrogen-bond inter-actions, linking the mol-ecules into chains along the a axis.
RESUMO
In the title compound, C(11)H(9)N(5)S, the dihedral angle between the mean planes of the thione-substituted triazole ring and benzonitrile ring is 4.28â (3)°. Inter-molecular N-Hâ¯S hydrogen bonds link the mol-ecules together into characteristic dimers.
RESUMO
In the title compound, [CoCl(2)(C(5)H(9)N(3))(2)], the Co(II) atom adopts a slightly distorted tetra-hedral coordination geometry provided by two chloride anions and two N atoms from the organic ligands. The dihedral angle between the pyrazole rings is 85.91â (10)°. In the crystal structure, mol-ecules are linked into a three-dimensional network by inter-molecular N-Hâ¯N and N-Hâ¯Cl hydrogen-bonding inter-actions.