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The Nyainqentanglha Metallogenic Belt (NMB) is an economically important lead-zinc ore province located in the Central and Southern Lhasa subterrane, Tibet. The NMB consists mainly of skarn-type lead-zinc polymetallic deposits that form at the contact between Late Cretaceous-Eocene intrusive rocks and carbonatite/volcanic-sedimentary strata. These deposits are generally considered to be related to S-type granites formed by the Indo-Asian continental collision. However, the εHf(t) (ave. -1.6) of zircons from the mineralization-related granitoids indicates that it is crust-mantle mixing products. In addition, the volcanic rocks of the Dianzhong Formation, which have a close spatio-temporal relationship with the skarn-type deposits, typically show mantle-derived features. Therefore, the occurrence of AFC processes in mantle-derived material passing through the thick basement of the Lhasa Terrane may better explain the genesis of the mineralization-related granitoids. In this study, we present results on zircon U-Pb ages, major and trace elements of the granitoids associated with skarn-type mineralization from the Narusongduo district. LA-ICP-MS zircon U-Pb dating shows that the granite formed in the Paleocene (58.6 ± 0.5 Ma). Combined with published regional data, we propose the mineralization age of the skarn-type deposits in the NMB ranges from the Late Cretaceous to Eocene. Detailed petrology and geochemistry of mineralization-related granitoids are evaluated to constrain the magmatic evolution process. The granitoids have high contents of SiO2 (mean 72.8 %), K2O (mean 4.0 %), Rb (mean 186.9 ppm), DI (differentiation index) 84.7, A/CNK (mean 1.3), and low contents of MgO, TFe, suggesting that the granitoids have undergone strong differentiation. In addition, the continuous decrease of P2O5 with progressive differentiation and the lower average P2O5 abundance suggest that the mineralization-related granitoids belong to the I-type granite. The results suggest that the mineralization-related granitoids in the NMB originated from the mantle and is the fractionated I-type granite formed by the process of AFC (Assimilation and Fractional Crystallization). Considering that Pb and Zn often coexist in deposits, we suggest that magmatic differentiation may play an important role in the formation of the granite-related Pb and Zn mineralization.
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Objective@#To investigate the clinical research of Guo's Liulian therapy in treating acute pancreatitis intestinal mucosal barrier dysfunction and provide reference for the patients with acute pancreatitis.@*Methods@#The 68 patients with acute pancreatitis who were admitted to our hospital from September 2016 to May 2018 were selected as study subjects. According to the random number table method, the patients were divided into the observation group and the control group, with 34 cases in each group. The patients in the control group were treated with conventional western medicine. The patients in the observation group were treated with Guo's Liulian therapy on the basis of the above treatment. The time of intestinal paralysis was compared between the two groups. The changes of intestinal mucosal barrier function and serum inflammatory factor related indexes before and after treatment were analyzed in the two groups. The difference of therapeutic effects between the two groups was observed.@*Results@#In the observation group, the abdominal pain relief time, abdominal distention relief time, bowel sound recovery time, and first bowel anus defecation time were significantly lower than those in the control group (t=7.621, 5.332 6.625, 8.762, all Ps<0.01). After treatment, serum LPS, DAO, ET, D-lactic acid, TNF-α, IL-1, IL-6, IL-8, and IL-10 and urine L/M were significantly lower in the observation group, while the serum ITF and MFG-E8 were significantly higher in the observation group (t=9.856, 6.974, 9.784, 16.068, 9.550, 12.506, 22.343, 16.625, 6.774, 23.469, 20.118, 23.031, all Ps<0.01).@*Conclusions@#The Guo's Liulian therapy treatment of acute pancreatitis in patients with intestinal mucosal barrier dysfunction can reduce the palsy remission time, reduce inflammation, increase serum ITF, MFG-E8 levels, repair of intestinal permeability, and improve the intestinal barrier function.
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Objective To investigate the clinical research of Guo's Liulian therapy in treating acute pancreatitis intestinal mucosal barrier dysfunction and provide reference for the patients with acute pancreatitis. Methods The 68 patients with acute pancreatitis who were admitted to our hospital from September 2016 to May 2018 were selected as study subjects. According to the random number table method, the patients were divided into the observation group and the control group, with 34 cases in each group. The patients in the control group were treated with conventional western medicine. The patients in the observation group were treated with Guo's Liulian therapy on the basis of the above treatment. The time of intestinal paralysis was compared between the two groups. The changes of intestinal mucosal barrier function and serum inflammatory factor related indexes before and after treatment were analyzed in the two groups. The difference of therapeutic effects between the two groups was observed. Results In the observation group, the abdominal pain relief time, abdominal distention relief time, bowel sound recovery time, and first bowel anus defecation time were significantly lower than those in the control group (t=7.621, 5.332 6.625, 8.762, all Ps<0.01). After treatment, serum LPS, DAO, ET, D-lactic acid, TNF-α, IL-1, IL-6, IL-8, and IL-10 and urine L/M were significantly lower in the observation group, while the serum ITF and MFG-E8 were significantly higher in the observation group (t=9.856, 6.974, 9.784, 16.068, 9.550, 12.506, 22.343, 16.625, 6.774, 23.469, 20.118, 23.031, all Ps<0.01). Conclusions The Guo's Liulian therapy treatment of acute pancreatitis in patients with intestinal mucosal barrier dysfunction can reduce the palsy remission time, reduce inflammation, increase serum ITF, MFG-E8 levels, repair of intestinal permeability, and improve the intestinal barrier function.
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BACKGROUND:Activation of Notch signaling plays a critical role in stem cel differentiation, and this effect seems to be cel-type dependent. Little is reported on the role of activation of Notch1 signaling in the differentiation of c-Kit+ bone marrow mesenchymal stem cels. OBJECTIVE:To analyze the influence of activation of Notch1 signaling on the differentiation of c-Kit+ bone marrow mesenchymal stem cels. METHODS:The Notch1 intracelular domain (N1-ICD) was obtained from the cDNA library by PCR and cloned intoBamHI/AgeI digested adenoviral GV314 plasmid to construct N1-ICD overexpressing shuttle plasmid, and the positive clones were verified by sequencing. N1-ICD shuttle plasmid and helper plasmids pBHGloxΔE1,3 Cre were used to co-transfect HEK293T cels to obtain N1-ICD overexpressing adenoviral particles (N1-ICD-Ad). The c-Kit+ subpopulation were isolated from bone marrow mesenchymal stem cels of the Sprague-Dawley rat femurviamagnetic activated cel sorting. After transfection of the c-Kit+ BMSCs with N1-ICD-Ad adenovirus, we assessed the activation of Notch1 signaling and differentiation in c-Kit+ bone marrow mesenchymal stem cels by quantitative RT-PCR and immunofluorescent staining. RESULTS AND CONCLUSION:N1-ICD coding sequence was successfuly generated from the cDNA library, and then was cloned into the linearized adenoviral vectors GV314. The resistant clones were verified by sequencing. With the assistance of packaging plasmids, recombinant N1-ICD-Ad adenovirus plasmids were successful packaged in HEK293T cels, and its title was 2×1012 PFU/L. c-Kit+ bone marrow mesenchymal stem cels with the purity of 91.6% were successfuly isolated from the bone marrow mesenchymal stem cels of the Sprague-Dawley rat femur. Compared with the blank and negative controls, N1-ICD-Ad infection in the c-Kit+ bone marrow mesenchymal stem cels led to substantial accumulation of N1-ICD in the cytoplasm and nuclei, significantly unregulated expressions of Hes1 (a downstream gene of Notch) and cardiomyocyte differentiation genes Nkx2.5 and cTnT, significantly increased the expression of von Wilebrand factor, an endothelial cel differentiation gene, and mildly increased the expression of smooth muscle22α, a smooth muscle cel differentiation gene. These experimental results indicate that the activation of Notch1 signaling contributes to multi-lineages differentiation of c-Kit+ bone marrow mesenchymal stem cels, and the construction of N1-ICD overexpressing adenoviral vector makes the foundation for further research on the role of Notch1 signaling in stem cel biology.
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RA is a self-immune disease mainly with chronic,from-head-to-foot,in-progress synovial arthropathy,whose internal reason is inefficiency liver blood,and dysfunctional liver also joins the attack course.It is treated in early,middle and late 3 stages,combining with trend on wind,coldness,wetness and hotness,respectively with corresponding drugs,as well as western medicine.Generally,it can be controlled within 6 months,with clinical relieving effect.
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Objective To discuss the curative effect and functionary mechanism of intraparotid injection on Sjogren’s syndrome (SS), validate the action of restoring the parotid configuration destroyed by immunoreaction and protecting unspoiled parotid, testify the action of improving the function of parotid excreting saliva by intraparotid injection. Methods Patients diagnosed as SS were divided into control group and treatment group. The patient of two groups were all treated by the same dosage hormone and Runzaoling capsule, at the same time, the treatment group were added the intraparotid injection. The parotid configuration, morphologic manifestation and functional melioration were observed. Result There was remarkable difference about the change of parotid configuration (t=1.67, P
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Objective To explore the effects and molecular mechanisms of arsenic trioxide(As 2O 3) in inducing apoptosis of colonic cancer cell line SW480. Methods The morphologic changes and apoptotic rate of SW480 cells induced by As 2O 3 were observed with fluorescent microscopy and flow cytometry. The bcl-2 and Fas expressions induced by As 2O 3 in SW480 cells were examined by immunohistochemical method. Results 24 hours after exposed to As 2O 3, SW480 cells exhibited typical morphologic changes of apoptosis, the apoptotic rate of which was 2.1%～10.6%. A typical subdiploid peak before G 1 phase was observed by flow cytometry, and As 2O 3 mainly acted in G 2/M phase. As 2O 3 decreased bcl-2 expression and increased Fas expression in SW480 cells. Conclusion As 2O 3 could induce apoptosis of SW480 cells. The molecular mechanism of As 2O 3-induced apoptosis of SW480 cells might be down-regulating the bcl-2 expression and up-regulating the Fas expression.
