RESUMO
Objective To investigate the expression of TFPT in non-small cell lung cancer (NSCLC), evaluate its clinical significance and study its influence on NSCLC. Methods Total of 50 specimens of carcinoma tissues and para-carcinoma tissue after surgery were collected.Then qRT-PCR was performed for TFPT expression. The correlations between the expression of TFPT and pathological characteristics were analyzed.pcDNA3.1-TFPT was transfected to up-regulate TFPT expression in A549 cells,and the transfection efficiency was evaluated by qRT-PCR.CCK8 assay was performed to evaluate the effects of ectopic TFPT on proliferation of A549 cells.The expression of TCF3 protein was determined by Western blot.Results The expression level of TFPT was up-regulated in tumor tissue compared with that in the para-carcinoma tissue. The expression of TFPT was associated with patients'gender, tumor size, differentiation degree, lymphatic metastasis and the distal metastasis (P < 0.01). Meanwhile, overexpression of TFPT increased the expression of TCF3 and the proliferation of A549 cells. Conclusions The expression of TFPT is obviously increased in NSCLC patients of female,larger tumor size, higher clinical stage or advanced histological grade. TFPT may promote cell proliferation by affecting the expression of TCF3 proteins.
RESUMO
The efficacy of the standard first-line chemotherapy regimen for advanced non-small cell lung cancer (NSCLC) has reached a plateau.Study confirmed that chemotherapy drugs and molecular targeted drug maintenance treatment can prolong the survival of patients with advanced NSCLC.Immunotherapy has become a maintenance treatment for advanced NSCLC,and further research is needed to confirm its efficacy.
