Simultaneously detect three illegal additive components in Yinhuang soft capsule by TLC-SERS / 药学实践杂志

Objective To establish a simultaneous detection of diclofenac sodium, fenbufen and rotundine hydrochloride illegally added in Yinhuang soft capsules by TLC-SERS. Methods The samples to be measured were placed on the silica gel plate, and the thin layer was developed with petroleum ether-ethyl acetate (3:5). Location detection was carried out under 254 nm ultraviolet lamp. Nano-silver colloidal solution was sprayed at each spot of separation according to the optimum conditions, and then qualitative identification was done by TLC-SERS. Results A method for simultaneous determination of diclofenac sodium, fenbufen and rotundine hydrochloride illegally added in Yinhuang soft capsule by TLC-SERS was established. And the minimum detection limits of three chemical constituents were determined. Conclusion TLC-SERS was a rapid, accurate and sensitive method for the simultaneous and rapid detection of diclofenac sodium, fenbufen and rotundine hydrochloride illegally added into Yinhuang soft capsule.

The sensitivity evaluation of Raman spectroscopy and near infrared spectroscopy ;in toxic adulterant detection of pharmaceutical excipient / 药学实践杂志

Objective To develop a rapid method for the detection of toxic adulterant (diethylene glycol) in pharmaceuti-cal excipient (glycerol) .Method The detection sensitivity of Raman/near infrared (NIR) spectroscopy combined with moving window correlation coefficient (MWCC) method was evaluated .Results The detection sensitivity of Raman spectroscopy was superior to that of NIR spectroscopy and with the assistance of MWCC method ;the sensitivity had been further improved . Conclusion Raman spectroscopy has the potential to become the effective method in the on-site detection of toxic adulterant in pharmaceutical excipient .

Modified local straight-line screening to detect synthetic drugs in adulterated herbal medicines.

A local straight-line screening (LSLS) algorithm was recently designed as a method to detect synthetic drug(s) in adulterated herbal medicines based on infrared spectroscopy. Some modifications are made in this paper to improve the existing LSLS algorithm, including interpolation, second derivation, and change of calculation regions from 3 to 7 data points. These modifications have decreased the effect of unpredicted noises and baseline shift on infrared spectroscopy, resulting in outstanding detailed spectral characteristics of the suspected synthetic drugs. The algorithm has been tested using five kinds of synthetic drugs (sibutramine, fenfluramine, lovastatin, sildenafil, and methyldopa) in 40 herbal medicine samples. The concentration of the synthetic drug(s) predicted by the modified LSLS algorithm is closer to those determined by high-performance liquid chromatography. Consequently, the correct results rise from 30 obtained using the original LSLS to 36 obtained using the modified LSLS in 40 samples, the false negative responses drop from 5 to 1, and the false positive responses drop from 5 to 3. The results obtained using the M-LSLS algorithm based on the sibutramine spectrum collected at different times and on different instruments also vary within acceptable ranges. These allow the method to be more appropriate for the preliminary screening of herbal medicines suspected of adulteration with synthetic drugs, with high rapidity, accuracy, and cost effectiveness.