COL15A1 interacts with P4HB to regulate the growth and malignancy of HepG2.2.15 cells.

Liver cancer is the fourth most common cause of cancer related deaths, ranking sixth in terms of incidence rate, and hepatocellular carcinoma (HCC) is the main type of liver cancer. Hepatitis B virus (HBV) infection is the main cause of HCC, and currently, HBV related HCC has become an important public health issue. COL15A1 encodes the alpha chain of collagen XV, a member of the FACIT collagen family, which has anti-angiogenic and anti-tumoral properties and play a vital role in tissue homeostasis in the liver, and its specific function in HBV-related HCC still needs further exploration. This study aimed to determine the regulatory role of COL15A1 in HBV-related HCC and explored the underlying mechanisms at the cellular level. Firstly, the biochip analysis results showed that the expression of COL15A1 was increased in human HBV-related HCC tissues. Furthermore, HBV induction also could significantly increase the expression of COL15A1 in hepatoma cell lines. Functionally, it found that COL15A1 silencing could significantly inhibit apoptosis and promote proliferation, migration, invasion and growth of HepG2.2.15. Mechanically, it found that COL15A1 could interact with P4HB，and its silencing could significantly increase the expression level of P4HB, thereby inhibiting the GRP76 expression and promoting growth and malignancy of HepG2.2.15 cells, revealing COL15A1 might play an anticancer role in HBV-related HCC.

Gradient selenium-doping regulating interfacial charge transfer in zinc sulfide/carbon anode for stable lithium storage.

Metal sulfides have attracted much attentions as anode materials for lithium-ion batteries (LIBs) because of the high theoretical capacity. However, the poor electronic conductivity and large volume variation usually give rise to the rapid capacity decay and undesirable rate performance, severely hampering their practical application. Herein, a gradient selenium-doped hollow sandwich structured zinc sulfide/carbon (ZnS/C) composite (Se-HSZC) is designed and fabricated as long life-span and stable anode material for LIBs. The gradient Se-doping enhances the interfacial charge transfer in Se-HSZC, while the unique double carbon shell sandwich structure further greatly reduces the volume expansion and ensures the electron fast transportation. Consequently, the Se-HSZC anode presents outstanding rate capability (654 mAh g-1 at 2 A g-1) with remarkable reversible capacity (567 mAh g-1 after 1500 cycles at 4 A g-1) for the half battery. In particular, a reversible capacity of 457 mAh g-1 at 0.5 A g-1 is achieved after 50 cycles for the full battery with LiNi0.6Co0.2Mn0.2O2 as cathode. This work offers a promising design route of novel metal sulfides nanostructures for high performance LIBs.