Development of a yeast cell based method for efficient screening of high yield tacrolimus production strain.

Tacrolimus (FK506) is a widely used and clinically important immunosuppressant drug that can be produced by fermentation of Streptomyces tsukubaensis. The industrial strains are typically obtained through multiple rounds of mutagenesis and screening, a labor-intensive process. We have established an efficient yeast cell based screening method for the evolutionary process of high-FK506-yielding strain. The S. tsukubaensis strains of different FK506 yields were tested for zone of growth inhibition of the wild type and calcineurin mutant (cnb1∆) yeast strains. We found that different FK506 yields correspond well to altered yeast growth inhibitions. Based on the combinational inhibition effects of FK506 with different antifungals that have been frequently reported, we also tested the zone of inhibition by addition of fluconazole, amphotericin B and caspofungin to the medium. In the end, for the best screening performance, we systemically evaluated the strategy when different yeast strains and different antifungals were used according to the clarity, size, and divergence of the inhibition circles. Using different yeast strains and antifungals, we successfully broadened the screening spectrum. An efficient high-FK506-yield S. tsukubaensis screening method has been established and optimized. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03870-y.

ADCdb: the database of antibody-drug conjugates.

Antibody-drug conjugates (ADCs) are a class of innovative biopharmaceutical drugs, which, via their antibody (mAb) component, deliver and release their potent warhead (a.k.a. payload) at the disease site, thereby simultaneously improving the efficacy of delivered therapy and reducing its off-target toxicity. To design ADCs of promising efficacy, it is crucial to have the critical data of pharma-information and biological activities for each ADC. However, no such database has been constructed yet. In this study, a database named ADCdb focusing on providing ADC information (especially its pharma-information and biological activities) from multiple perspectives was thus developed. Particularly, a total of 6572 ADCs (359 approved by FDA or in clinical trial pipeline, 501 in preclinical test, 819 with in-vivo testing data, 1868 with cell line/target testing data, 3025 without in-vivo/cell line/target testing data) together with their explicit pharma-information was collected and provided. Moreover, a total of 9171 literature-reported activities were discovered, which were identified from diverse clinical trial pipelines, model organisms, patient/cell-derived xenograft models, etc. Due to the significance of ADCs and their relevant data, this new database was expected to attract broad interests from diverse research fields of current biopharmaceutical drug discovery. The ADCdb is now publicly accessible at: https://idrblab.org/adcdb/.

The effectiveness of taught, self-help mindfulness-based interventions on Chinese adolescents' well-being, mental health, prosocial and difficult behavior, and coping strategy.

Despite China's enduring historical connection with mindfulness and the growing recognition of mindfulness-based interventions (MBIs) in Western education, mindfulness remains relatively underexplored in the Chinese education system. This study addresses the scarcity of resources and certified instructors in China by assessing the effectiveness of MBIs in both taught and self-help forms in improving well-being and prosocial behavior and regulating negative emotions and behaviors among Chinese adolescents. The analysis included 362 Chinese students (mean age = 13.03, SD = 0.50, 47.5% girls) from 12 classes, assigned to the taught MBI group (N = 129, post), self-help MBI group (N = 116, post), or control group (N = 117, post). Paired-sample t-tests and multi-level modeling were used, accounting for data nesting and incorporating gender, whether students live in school, and age as covariates. Results revealed a significantly greater improvement in well-being in the taught group compared with the control, with both MBI groups demonstrating increased prosocial behavior. While the taught group showed a significant decrease in loneliness, it was accompanied by an increase in internalizing problems. Coping strategies varied across the groups, with no significant changes in depression, anxiety, and stress levels. These findings imply the potential value of integrating MBIs into the Chinese educational system, especially given the self-help approach's favorable outcomes.

The Association Between Food Addiction and Weight Status in School-Age Children and Adolescents.

Background: The association between food addiction (FA) and weight status in children and adolescents remains poorly understood. This study aimed to elucidate the association between FA and weight status using the validated Chinese version of the dimensional Yale Food Addiction Scale for Children 2.0 (dYFAS-C 2.0). Methods: Participants were enrolled from clinic visitors for regular physical check in a children's hospital. The dYFAS-C 2.0 was translated into Chinese and validated using reliability and validity tests. The participants' body mass index Z score (BMIZ) and waist-to-height ratio (WHtR) were used to characterize weight status. The FA severity was assessed using the translated dYFAS-C 2.0. Results: Among the 903 children and adolescents enrolled, 426 (47.2%) completed the survey [277 (65%) females and 149 (35%) males]. The Cronbach α of translated dYFAS-C 2.0 was 0.934, and confirmatory factor analysis indicated an acceptable model fit. FA correlated positively with BMIZ and WHtR in the whole sample after adjusting for the effect of gender (p < 0.001). Further analyses showed that the correlation remained significant in participants with BMIZ > 1 (p = 0.006) but not in those with BMIZ ≤ 1 (p = 0.220). However, the correlations between FA and WHtR were statistically significant in both participants with or without abdominal obesity (p < 0.05). The FA could explain 12.1 and 15.8% of variance in BMIZ and WHtR, respectively. The corresponding cutoff points of FA for excessive weight risk were 0.7 (BMIZ) and 0.4 (WHtR). Conclusion: The dYFAS-C 2.0 has good reliability and validity in the Chinese population. FA is associated with weight status characterized by BMIZ and WHtR, especially in participants with BMIZ > 1 and in those with abdominal obesity. Clinical Trial Registration: [www.chictr.org.cn], identifier [ChiCTR2100052239].

Detection of candidate gene networks involved in resistance to Sclerotinia sclerotiorum in soybean.

Quantitative trait locus (QTL) mapping often yields associations with dissimilar loci/genes as a consequence of diverse factors. One trait for which very limited agreement between mapping studies has been observed is resistance to white mold in soybean. To explore whether different approaches applied to a single data set could lead to more consistent results, haplotype-trait association and epistasis interaction effects were explored as a complement to a more conventional marker-trait analysis. At least 10 genomic regions were significantly associated with Sclerotinia sclerotiorum resistance in soybean, which have not been previously reported. At a significance level of α = 0.05, haplotype-trait association showed that the most prominent signal originated from a haplotype with 4-SNP (single nucleotide polymorphism) on chromosome 17, and single SNP-trait analysis located a nucleotide polymorphism at position rs34387780 on chromosome 3. All of the peak-SNPs (p-value < 0.05) of each chromosome also appeared in their respective haplotypes. Samples with extreme phenotypes were singled-out for association studies, 25-30% from each end of the phenotypic spectrum appeared in the present investigation to be the most appropriate sample size. Some key genes were identified by epistasis interaction analysis. By combining information on the nearest positional genes indicated that most loci have not been previously reported. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses suggest potential candidate genes underlying callose deposition in the cell wall and mitogen-activated protein kinase (MAPK) signaling pathway-plant, as well as plant-pathogen interaction pathway, were activated. Integration of multi-method genome-wide association study (GWAS) revealed novel genomic regions and promising candidate genes in novel regions, which include Glyma.01g048500, Glyma.03g129100, Glyma.17g072200, and the Dishevelled (Dvl) family of proteins on chromosomes 1, 3, 17, and 20, respectively.

Correction: Construction of PtSe2/Ge heterostructure-based short-wavelength infrared photodetector array for image sensing and optical communication applications.

Correction for 'Construction of PtSe2/Ge heterostructure-based short-wavelength infrared photodetector array for image sensing and optical communication applications' by Yu Lu et al., Nanoscale, 2021, 13, 7606-7612, DOI: 10.1039/D1NR00333J.

Examining Social Status Profiles with Gender, School Attended, SES, Academic Achievement and Wellbeing in Urban China.

Previous research has produced inconsistent findings about the relationships between aggressive and prosocial behavior with likeability and popularity. This study utilized latent profile analysis to identify naturally occurring social status profiles with these indicators and to explore their associations with gender, school attended, subjective social status, academic achievement, and wellbeing. The study recruited 818 (aged 12-15 years, 46% girls) Chinese adolescents and revealed four unique social status profiles: high aggressive-low likeability, low social status, average, and high prosocial-high social status groups. A bi-strategic profile did not emerge. The low social status and high aggressive groups exhibited the lowest academic achievement and wellbeing suggesting that more attention should be devoted to these students both in school and in future research.

Construction of PtSe2/Ge heterostructure-based short-wavelength infrared photodetector array for image sensing and optical communication applications.

In this work, we present the construction of a multilayered PtSe2/Ge heterostructure-based photodetector array comprising 1 × 10 device units operating in the short-wavelength infrared (SWIR) spectrum region. The as-fabricated heterostructures show an obvious photovoltaic effect, providing the devices with the ability to work as self-driven photodetectors. Upon 1550 nm illumination, a typical photodetector exhibits prominent photoresponse performance with the current on/off ratio, responsivity, external quantum efficiency and specific detectivity reaching 1.08 × 103, 766 mA W-1, 61.3% and 1.1 × 1011 Jones, respectively. The device also has a fast response speed with rise/fall times of 54.9 µs/56.6 µs. Thanks to the respectable homogeneity in device performance, the photodetector array can reliably record an image of a "diode symbol" produced by SWIR irradiation. What is more, the photodetector is successfully integrated into a SWIR optical communication system serving as an optical receiver to transmit a text signal. The above results imply a huge possibility of the present heterostructure-based photodetector array for some optoelectronic purposes such as SWIR image sensing and optical communication applications.

Comparative proteomics analysis reveals differentially accumulated proteins associated with male and female A. chinensis var. chinensis bud development.

BACKGROUND: Kiwifruit (Actinidia chinensis var. Chinensis) is abundant with vitamin C and is a rapidly developing crop in China, New Zealand, and other countries. It has been widely used as a raw material for food and kiwifruit wine. Among these, A. chinensis var. chinensis and A. chinensis var. deliciosa are the most valuable kiwifruit in production. Kiwifruit is a typical dioecious plant and its female and male plants have different economic values. Therefore, sex identification, especially at the seedling stage, has important implications for the scientific planning of its production and economic benefits. However, the kiwifruit sex regulation mechanism is very complex and molecular studies are in the initial stages. Currently, there is not a universal and effective sex identification method for A. chinensis. METHODS: In this study, we used a label-free quantitative proteomics approach to investigate differentially accumulated proteins, including their presence/absence and significantly different levels of abundances during A. chinensis var. chinensis male and female flower bud development. RESULTS: A total of 6485 proteins were identified, among which, 203 were identified in male buds, which were mainly associated with phenylalanine metabolism, tyrosine metabolism, and plant hormone signal transduction. In female buds, 241 were identified, which were mainly associated with the ErbB signaling pathway, growth hormone synthesis, secretion and action, and mRNA surveillance pathway. A total of 373 proteins were significantly differentially accumulated proteins (fold change > 2; P < 0.05), of which, 168 were upregulated and 205 were downregulated. Significant differences between proteins involved 13 signaling pathways, most of which were involved in flavonoid biosynthesis, phenylpropanoid biosynthesis, and starch and sucrose metabolism. Protein interaction analysis showed that enriched protein nodes included cell division cycle 5-like protein, 40S ribosomal protein S8, ribosomal protein, and 40S ribosomal protein like, which interact with 35, 25, 22, and 22 proteins, respectively. CONCLUSIONS: This study provide valuable information for cloning key genes that control sex traits and functionally analyze their roles, which lay a foundation to the development of molecular markers for male and female kiwifruit identification.

Recovery of gold from waste mobile phone circuit boards and synthesis of nanomaterials using emulsion liquid membrane.

In recent years, waste mobile phones have become popular as electronic waste due to the huge amount, serious pollution with improper disposal and high resource value. It is imperative to realize the recycling of resources in waste mobile phones. The application of emulsion liquid membrane in the recovery of gold and synthesis of nanomaterials from waste mobile phone printed circuit boards (WMPCBs) was studied. The components of the emulsion liquid membrane, effects of 7 factors on the extraction rate and the morphology of the synthesized nanomaterials were explored. The results show that it is possible to extract 99.79% of Au(III) from WMPCBs leachate with kerosene as diluent, Span80 as surfactant, methyl isobutyl ketone(MIBK) as carrier, ascorbic acid solution as stripping agent and liquid paraffin as membrane stabilizer. The external phase pH and the internal phase concentration had a greater influence on the extraction efficiency of Au(III) among 7 factors. The morphology of the synthesized product was affected by the concentration and type of the surfactant. It provides a new idea to connect recovery from waste with deep processing, extending the waste mobile phone recycling process chain and achieving high-value utilization of waste mobile phone secondary metal resource products.

Adsorption of gold from waste mobile phones by biochar and activated carbon in gold iodized solution.

The application of laboratory-generated biochar and activated carbon adsorbents in gold iodized solution for the recycling of waste mobile phone printed circuit boards (WMPCBs) is investigated. This research aims to solve problems associated with the existing gold recovery technologies of WMPCBs. Currently, the disposal of WMPCBs is expensive, involves complex processes, and contributes to secondary pollution. In this study, laboratory-generated biochar is produced from corn straw, wheat straw, and wood chips by pyrolysis. The effects of factors on the adsorption efficiency are investigated, and the optimal operating conditions for biochar and activated carbon adsorption are determined. The following optimal parameters were found for activated carbon: temperature = 25 °C, particle size = 40-60 mesh, dosage = 0.05 g/10 mL, pH = 7, reaction time = 2 h, and oscillation frequency = 200 r/min. The adsorption efficiency reached 98.6%. For biochar, optimization involved: raw material from corn straw at a pyrolysis temperature = 700 °C, reaction time = 5 h, oscillation frequency = 200 r/min, pH = 3, dosage = 0.15 g/10 mL, and temperature = 50 °C. An adsorption efficiency of 98% was achieved. The two adsorbents were compared, and results demonstrated that the adsorption properties of the laboratory-generated biochar were slightly inferior to those of the activated carbon; however, they were similar. Biochar adsorption can reuse waste, which may not only solve the current problems related to WMPCB recycling, but can help to achieve a "win-win" situation of increased environmental protection and sustainable utilization of resources.

Does Immigrant Generation Matter? Re-examining the Ethnic Density Effects on Mental Health of Ethnic Minorities in Britain.

Objectives: In this study, we extend previous research by re-examining the ethnic density effects on mental health of British ethnic minorities and exploring how the relationship varies across generations at a lower geographic scale Lower Super Output Areas (official census geographical classification designed for the consistent reporting of local statistics). Methods: We used random intercept logistic multilevel models to analyze the second wave (2010-2011) of the United Kingdom Household Longitudinal Study (UKHLS). Results: The results show that after controlling for neighborhood deprivation, respondents' socio-demographic characteristics, duration of stay in a neighborhood and moving preference, ethnic concentration has a detrimental effect on mental health for Pakistanis, Bangladeshis, black Caribbeans and black Africans. Moreover, the results show that the detrimental effects are particularly pronounced for the first-generation Pakistanis, Bangladeshis and Indians compared to their second-generation counterparts. Conclusions: Policy interventions to ameliorate ethnic disadvantages in mental health may need to be more targeted to first-generation South Asian minorities living in ethnically clustered areas, and that previous research overlooking migration generation may conceal important internal differences within British ethnic minorities.

An efficient utilization of chromium-containing vanadium tailings: Extraction of chromium by soda roasting-water leaching and preparation of chromium oxide.

Chromium-containing vanadium tailings (CCVT), an industrial waste, were utilized to extract chromium efficiently by soda roasting-water leaching process and for the preparation of highly pure chromium oxide. The effect of extraction of chromium under different roasting and leaching conditions were analyzed using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The maximum chromium extraction rate of 91.51% was obtained when soda (Na2CO3) and CCVT were mixed in a molar ratio (n (Na2CO3)/n (Cr2O3)) of 8, roasted at 900 °C and maintained for 120 min. Then, the roasted product was leached in water at 60 °C for 60 min with a liquid-solid mass ratio (L/S) of 10. During soda roasting, the chromium-containing phase (Fe0.6Cr0.4)2O3 combines with Na2CO3 to form Na2CrO4, which was then transferred into the leaching liquid, post water leaching. The by-products such as NaFeTiO4, Na2CaSiO4, and Na0.68Fe0.68Si0.32O2 were left in the leaching residue which was called chromium tailings (CT). 87.40% chromium oxide was recovered from the unpurified leaching liquid after reduction and precipitation by adding Na2S, followed by roasting the deposit. This process not only relieved the potential threat of the industrial waste CCVT to the environment but also realized the recovery of the valuable element chromium.

Absent Pulmonary Valve Syndrome in a Fetus: A Case Report and Literature Review.

INTRODUCTION: The main characteristics of absent pulmonary valve syndrome (APVS) include the absence or hypoplasia of the pulmonary valve, stenosis of the pulmonary valve annulus, and aneurysmal dilatation of the pulmonary trunk and its branches. In the more common type 1, the tetralogy of Fallot-like type, there is a ventricular septal defect, overriding aorta, pulmonary arterial dilatation, and absence of ductus arteriosus, The second type has an intact ventricular septum, less pulmonary artery dilatation, and a patent ductus arteriosus, with or without tricuspid atresia. CASE REPORT: This APVS had an intact ventricular septum with an absent ductus arteriosus. CONCLUSION: The APVS with intact ventricular septum with an absent ductus arteriosus may represent a third type of APVS.