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Food Res Int ; 183: 114202, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38760133

RESUMO

Pixian broad bean paste is a renowned fermented seasoning. The fermentation of broad bean is the most important process of Pixian broad bean paste. To enhance the flavor of tank-fermented broad bean paste, salt-tolerant Bacillus amyloliquefaciens strain was inoculated, resulting in an increase in total amount of volatile compounds, potentially leading to different flavor characteristics. To investigate the fermentation mechanism, monoculture simulated fermentation systems were designed. Metabolomics and transcriptomics were used to explore Bacillus amyloliquefaciens' transcriptional response to salt stress and potential aroma production mechanisms. The results highlighted different metabolite profiles under salt stress, and the crucial roles of energy metabolism, amino acid metabolism, reaction system, transportation system in Bacillus amyloliquefaciens' hypersaline stress response. This study provides a scientific basis for the industrial application of Bacillus amyloliquefaciens and new insights into addressing the challenges of poor flavor quality in tank fermentation products.


Assuntos
Bacillus amyloliquefaciens , Fermentação , Metabolômica , Bacillus amyloliquefaciens/metabolismo , Bacillus amyloliquefaciens/genética , Transcriptoma , Microbiologia de Alimentos , Alimentos Fermentados/microbiologia , Compostos Orgânicos Voláteis/análise , Compostos Orgânicos Voláteis/metabolismo , Perfilação da Expressão Gênica , Paladar , Fabaceae/microbiologia
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