The Long Non-Coding RNA AC006329.1 Facilitates Hepatocellular Carcinoma Progression and Metastasis by Regulating miR-127-5p/SHC3/ERK Axis.

Purpose: Hepatocellular carcinoma(HCC) is the most common type of liver cancer and the sixth largest common cancer worldwide. Although surgical resection, hepatic arterial chemoembolization, targeted drugs and immunotherapy are currently available, the mortality of advanced patients remains high. Therefore, new therapeutic targets are urgently needed. In recent years, many studies have found that The long non-coding RNA(lncRNA) has multiple functions in human tumors, including participating in epigenetic, transcriptional, post-transcriptional and translational regulation, and is closely related to the progression of HCC. The purpose of this study was to investigate the role of AC006329.1 in HCC progression and provide theoretical guidance for finding new targets. Patients and Methods: AC006329.1 was screened out by transcriptome sequencing and quantitative real-time polymerase chain reaction (qRT-PCR). Then a series of functional tests in vivo and in vitro were conducted to investigate the effects of AC006329.1 on HCC progression and metastasis. Epithelial-mesenchymal transformation (EMT) of HCC was detected by Western blot and immunofluorescence staining. The targeted miRNA and downstream gene of AC006329.1 were predicted by databases and the pathway regulation axis eventually validated by dual luciferase reporter assays, qRT-PCR and WB. Results: AC006329.1 was found high expressed in HCC tissues and cell lines by qRT-PCR. The prognosis of HCC patients with high expressed AC006329.1 was poor. In vitro and in vivo, overexpression of AC006329.1 can promote the progression, metastasis and EMT of HCC by acting as a sponge of miR-127-5p to increase the expression of SHC3. In addition, up-regulation of miR-127-5p or knockdown of SHC3 can both reverse the promoting effects of AC006329.1 on progression, metastasis and EMT of HCC. Finally, WB and qRT-PCR analysis was discovered that AC006329.1 can facilitate HCC progression, EMT and metastasis by competitively inhibiting miR-127-5p to activate SHC3/ERK signaling pathway. Conclusion: These above experimental results confirmed that AC006329.1 can facilitate HCC progression, EMT and metastasis by acting as a competing endogenous RNA (ceRNA) to inhibit miR-127-5p and activate SHC3/ERK signaling pathway.

Enhancing the Activity of Surface Immobilized Antimicrobial Peptides Using Thiol-Mediated Tethering to Poly(ethylene glycol).

Considering the need for versatile surface coatings that can display multiple bioactive signals and chemistries, the use of more novel surface modification methods is starting to emerge. Thiol-mediated conjugation of biomolecules is shown to be quite advantageous for such purposes due to the reactivity and chemoselectivity of thiol functional groups. Herein, the immobilization of poly(ethylene glycol) (PEG) and antimicrobial peptides (AMPs) to silica colloidal particles based on thiol-mediated conjugation techniques, along with an assessment of the antimicrobial potential of the functionalized particles against Pseudomonas aeruginosa and Staphylococcus aureus is investigated. Immobilization of PEG to thiolated Si particles is performed by either a two-step thiol-ene "photo-click" reaction or a "one-pot" thiol-maleimide type conjugation using terminal acrylate or maleimide functional groups, respectively. It is demonstrated that both immobilization methods result in a significant reduction in the number of viable bacterial cells compared to unmodified samples after the designated incubation periods with the PEG-AMP-modified colloidal suspensions. These findings provide a promising outlook for the fabrication of multifunctional surfaces based upon the tethering of PEG and AMPs to colloidal particles through thiol-mediated biocompatible chemistry, which has potential for use as implant coatings or as antibacterial formulations that can be incorporated into wound dressings to prevent or control bacterial infections.

Microparticles of High Entropy Alloys Made by Laser-Induced Forward Transfer.

The controlled deposition of CoCrFeNiMo0.2 high-entropy alloy (HEA) microparticles was achieved by using laser-induced forward transfer (LIFT). Ultra-short laser pulses of 230 fs of 515 nm wavelength were tightly focused into ∼2.4 µm focal spots on the ∼50-nm thick plasma-sputtered films of CoCrFeNiMo0.2. The morphology of HEA microparticles can be controlled at different fluences. The HEA films were transferred onto glass substrates by magnetron sputtering in a vacuum (10-8 atm) from the thermal spray-coated substrates. The absorption coefficient of CoCrFeNiMo0.2α≈6×105 cm-1 was determined at 600-nm wavelength. The real and imaginary parts of the refractive index (n+iκ) of HEA were determined from reflectance and transmittance by using nanofilms.

[Quantification on driving forces for spatiotemporal evolution of precipitation-use efficiency of grassland across recent two decades in Otog Banner, Inner Mongolia, China]. / 近20å¹´å† è’™å¤é„‚æ‰˜å ‹æ——è‰åœ°é™æ°´åˆ©ç”¨æ•ˆçŽ‡æ—¶ç©ºæ¼”å˜çš„é©±åŠ¨åŠ›é‡åŒ–.

Precipitation use efficiency (PUE) is an effective index to evaluate the relationship between grassland productivity and precipitation in arid and semi-arid regions. To explore the driving mechanism of climate change and human activities on grassland PUE, we used the improved CASA model to estimate net primary productivity (NPP) of grassland from 2001 to 2020 in Otog Banner, Inner Mongolia. The PUE was obtained combining with the spatial interpolation data of precipitation. The spatiotemporal evolution of PUE and its responses to the six climate factors were analyzed using simple and piecewise linear regression. A quantitative analysis method based on partial derivatives was used to quantitatively evaluate the relative contributions of climate change and human activities to PUE dynamics. The results showed that the annual average value of PUE was 0.748 g C·m-2·mm-1 in Otog Banner, and that the inter-annual fluctuation had a significant downward trend at a rate of 0.014 g C·m-2·mm-1·a-1. Across the study area, PUE increased from west part to east part, and exhibited significant single-peak piecewise linear patterns along the growth gradients of temperature, precipitation, relative humidity, sunshine hours, and ET0. There was a sustained and significant increase pattern of fast first and then slow along the wind speed gradient. 94.3% of the grassland in the study area showed a decrease trend in PUE, and 43.6% area showed severely decreased. This prominent decrease in PUE was co-driven by climate change and human activities, whose contributions were -1.162×10-2 and -0.240×10-2 g C·m-2·mm-1·a-1, respectively. Climate change was the primary driving force and precipitation was the key climate driving factor for the decrease in PUE.

Two-Photon Dual-Emissive Carbon Dot-Based Probe: Deep-Tissue Imaging and Ultrasensitive Sensing of Intracellular Ferric Ions.

Carbon dots (CDs)-based nanoparticles have been extensively explored for biological applications in sensing and bioimaging. However, the major translational barriers to CDs for imaging and sensing applications include synthetic strategies to obtain monodisperse CDs with tunable structural, electronic, and optical properties in order to achieve high-resolution deep-tissue imaging, intracellular detection, and sensing of metal ions with high sensitivity down to nanomolar levels. Herein, we report a novel strategy to synthesize and develop a multifunctional nitrogen-doped CDs probe of different sizes using a new combination of carbon and nitrogen sources. Our results show that the structural characteristics (i.e., the surface density of emissive traps and bandgaps levels) depend on the size of the CDs, which ultimately influences their optical properties. This work also demonstrates the development of a two-photon dual-emissive fluorescent multifunctional probes (3-FCDs) by conjugating fluorescein isothiocyanate on the surface of nitrogen-doped CDs. 3-FCDs show excellent near-infrared two-photon excitation ability, single-wavelength excitation, high photostability, biocompatibility, low cytotoxicity, and good cell permeability. Using two-photon fluorescence imaging, our multifunctional probe shows excellent deep-tissue high-resolution imaging capabilities with penetration depth up to 3000 and 280 µm in hydrogel scaffold and pigskin tissue, respectively. The designed probe exhibits ultrasensitivity and specificity toward Fe3+ ions with a remarkable detection limit of 2.21 nM using two-photon excitation. In addition, we also demonstrate the use of multifunctional CDs probe for ultrasensitive exogenous and real-time endogenous sensing of Fe3+ ions and imaging in live fibroblasts with rapid response times for intracellular ferric ion detection.

Influence of charged defects on the interfacial bonding strength of tantalum- and silver-doped nanograined TiO2.

A nano-grained layer including line defects was formed on the surface of a Ti alloy (Tialloy, Ti-6Al-4V ELI). Then, the micro- and nano-grained Tialloy with the formation of TiO2 on its top surface was coated with a bioactive Ta layer with or without incorporating an antibacterial agent of Ag that was manufactured by magnetron sputtering. Subsequently, the influence of the charged defects (the defects that can be electrically charged on the surface) on the interfacial bonding strength and hardness of the surface system was studied via an electronic model. Thereby, material systems of (i) Ta coated micro-grained titanium alloy (Ta/MGTialloy), (ii) Ta coated nano-grained titanium alloy (Ta/NGTialloy), (iii) TaAg coated micro-grained titanium alloy (TaAg/MGTialloy) and (iv) TaAg coated nano-grained titanium alloy (TaAg/NGTialloy) were formed. X-ray photoelectron spectroscopy was used to probe the electronic structure of the micro- and nano-grained Tialloy, and so-formed heterostructures. The thin film/substrate interfaces exhibited different satellite peak intensities. The satellite peak intensity may be related to the interfacial bonding strength and hardness of the surface system. The interfacial layer of TaAg/NGTialloy exhibited the highest satellite intensity and maximum hardness value. The increased bonding strength and hardness in the TaAg/NGTialloy arises due to the negative core charge of the dislocations and neighbor space charge accumulation, as well as electron accumulation in the created semiconductor phases of larger band gap at the interfacial layer. These two factors generate interfacial polarization and enhance the satellite intensity. Consequently, the interfacial bonding strength and hardness of the surface system are improved by the formation of mixed covalent-ionic bonding structures around the dislocation core area and the interfacial layer. The bonding strength relationship by in situ XPS on the metal/TiO2 interfacial layer may be examined with other noble metals and applied in diverse fields.

Usefulness of Deep Hypothermic Circulatory Arrest and Regional Cerebral Perfusion in Children.

To compare the safety and usefulness of deep hypothermic circulatory arrest (DHCA) and regional cerebral perfusion (RCP) during pediatric open heart surgery. Between January 1, 2004 and September 30, 2012, 1250 children with congenital cardiac defect underwent corrective operation with the DHCA or RCP technique in the Shanghai Children's Medical Center. Of them, 947 cases underwent the operation with the aid of DHCA (DHCA group), and 303 cases with RCP (RCP group). The mean DHCA time was 30.64±15.81 (7-63) minutes and mean RCP time was 36.18±12.86 (10-82) minutes. The mortality rate was 7.18% (68/947) and 6.60% (20/30) in two groups, respectively. The postoperative incidences of temporary and permanent neurological dysfunction were 6.23% (59/947) in the DHCA group and 2.64% (8/303) in the RCP group (p<0.01). The incidence of other complications such as low cardiac output, renal dysfunction, and lung issues are similar in both groups. RCP is a reliable technique for cerebral protection and it facilitates time-consuming corrected procedures for complex congenital cardiac defect repair procedures.

[Chinese medicinal culture in Tianmu Mountain before 1949].

According to the Annals of Western Tianmu Mountain, the medicinal plants in Tianmu Mountain reached as many as 1 200 types, with diversified species and excellent qualities, hence, the titles of "valuable thesaurus of Tianmu Mountain." The rich medicinal resources promotes the flourishing of local trading of crude medicines. From 1931 to 1940, based on the survey of the rural economy in Lin'an, Yuqian, and Changhua counties, there were 97 drugstores. Moreover, there are lots of folk stories and poems about medical herbs in Tianmu Mountain. Therefore, Tianmu Mountain is world-renowned because of its abundant resources of medical herbs, and the prosperous medicinal trading offered rich source materials for literature writings.

Pulsatile flow improves cerebral blood flow in pediatric cardiopulmonary bypass.

The objective of this study was to evaluate the effect of pulsatile flow on cerebral blood flow (CBF) in infants with the use of a mild hypothermic cardiopulmonary bypass (CPB). Thirty infants scheduled for open heart surgery were randomized to the pulsatile group (Group P, n = 15) and nonpulsatile group (Group NP, n = 15). In Group P, pulsatile perfusion was applied during the aortic cross-clamping period, whereas nonpulsatile perfusion was used in Group NP. The systolic peak velocity (Vs), the end of diastolic velocity (Vd), the mean velocity (Vm), and the pulsatility index (PI) and the resistance index (RI) of the middle cerebral artery were measured by a transcranial Doppler (TCD) ultrasound after anesthesia (T1; baseline), at the beginning of CPB (T2), 10 min after aortic cross-clamping (T3), 3 min after declamping (T4), at the cessation of CPB (T5), and at the end of the operation (T6). During T3 and T4, the Vs in Group P was significantly higher than in Group NP. However, there were no statistically significant differences between Vd and Vm. The PI and RI in Group P were also higher than those in Group NP (both P < 0.05). During T5, Vd and Vm were higher in Group P (P < 0.05), whereas there was no difference in Vs. Additionally, PI and RI in Group P were significantly lower than those in Group NP (P < 0.05). However, there was no difference during T6. Pulsatile perfusion may increase CBF and decrease cerebral vascular resistance in the early period after mild hypothermic CPB.

Development of mechanical circulatory support devices in China.

Myocardial dysfunction leading to low cardiac output syndrome is a common clinical pathophysiological state. Currently, the use of mechanical circulatory support (MCS) is an essential aspect of the treatment of patients with cardiac failure. Several groups in China are engaged in the design and development of MCS devices. These devices can be classified as pulsatile, rotary, and total artificial heart (TAH). There are two types of pulsatile pump, which are driven by air (pneumatic). One of these pumps, the Luo-Ye pump, has been used clinically for short-term support since 1998. The other is a push-plate left ventricular device, which has a variable rate mode. Various rotary devices are classified into axial and centrifugal pumps, depending on the impeller geometry. Most rotary pumps are based on the maglev principle, and some types have been used clinically. Others are still being studied in the laboratory or in animal experiments. Furthermore, certain types of total implantable pump, such as the UJS-III axial pump and the UJS-IV aortic valvo-pump, have been developed. Only one type of TAH has been developed in China. The main constituents of this artificial heart are two axial pumps, two reservoir tanks mimicking the right and left atria, flow meters, two pressure gauges, and a resistance adaptor. Although the development of mechanical assist devices in China is still in a nascent stage, a number of different types of MCS devices are currently being studied.

[Application of spatial autocorrelation analysis to the COD, SO2 and TSP emission in Jiangsu province].

Spatial autocorrelation is an effective tool of spatial statistics, which is used to disclose the spatial structure of regional disparity. There are two different scales to measure regional spatial dependence: global spatial autocorrelation and local spatial autocorrelation. Based on environmental data of 13 cities in Jiangsu province from 1990 to 2006, the regional disparity of COD, SO2 and TSP emission was discussed by using spatial autocorrelation analysis methods. The results show that total emission of COD and TSP decreased respectively from 596 353 t and 1 101 404 t in 1990 to 291 762 t and 704734 t in 2006, while total emission of SO2 kept steady. In 2006, Global Moran's I of COD, SO2 and TSP emission was 0.465 7, 0.214 2 and 0.510 1 respectively. It is identified that positive spatial autocorrelation is presented and spatial aggregation pattern of COD, SO2 and TSP emission are appeared. However, spatial aggregation pattern of COD emission appears earlier than that of SO2 and TSP, and spatial aggregation degree of COD is also higher than that of SO2 and TSP. There are different spatial patterns between southern and northern Jiangsu. In southern Jiangsu, Global Moran's I of COD, SO2 and TSP emission had increased to 0.499 7, 0.320 2 and 0.298 3 up to 2006, and spatial aggregation pattern appeared remarkably. In northern Jiangsu, most of the Global Moran's I were less than -0.2, and spatial aggregation pattern disappeared accordingly. High cluster region of COD emission is Suzhou, Wuxi and Changzhou, and high cluster region of SO2 emission is Suzhou and Wuxi. However, spatial pattern of TSP emission does not change much and five cities of southern Jiangsu (Suzhou, Wuxi, Changzhou, Zhenjiang, Nanjing) are still the high cluster region. The last, the research provides an important cognition to regional environment disparity and macro-environmental strategy, and a significant means to harmonious society and eco-province construction in Jiangsu province.

[Analysis of primary elemental speciation distribution in mungbean during enzymatic hydrolization].

In the present paper, trace elements contents of cuprum, zincum, manganese and ferrum in mungbean and their primary speciation distribution during enzymatic hydrolization were investigated with ICP-AES OPTIMA 5300DV plasma emission spectroscopy. The trace elements were separated into two forms, i.e. dissolvable form and particulate form, by cellulose membrane with 0.45 microm of pore diameter. All the samples were digested by strong acid (perchloric acid and nitric acid with 1 : 4 ratio ). The parameters of primary speciations of the four elements were calculated and discussed. The results showed: (1) Contents of cuprum, zincum, manganese and ferrum in mungbean were 12.77, 31.26, 18.14 and 69.38 microg x g(-1) (of dry matter), respectively. Different treatment resulted in different elemental formulation in product, indicating that more attention should be paid to the trace elements pattern when producing mungbean beverage with different processes. (2) Extraction rates of cuprum, zincum, manganese and ferrum in extract were 68.84%, 51.84%, 63.97% and 30.40% with enzymatic treatments and 36.22%, 17.58%, 7.85% and 22.99% with boil treatment, respectively. Both boil and enzymatic treatments led to poor elemental extraction rates, which proved that it was necessary to take deep enzymatic hydrolysis treatment in mungbean beverage process as the trace element utilization rate was concerned. (3) Amylase, protease and cellulose showed different extraction effectiveness of the four trace elements. Generally, protease exhibited highest efficiency for the four elements extraction. All of the four trace elements were mostly in dissolvable form in all hydrolysates and soup. (4) Relative standard deviations and recovery yields are within 0.12%-0.90% (n = 11) and 98.6%-101.4%, respectively. The analysis method in this paper proved to be accurate.

Effect of flow rate, negative pressure, and duration of modified ultrafiltration on hemodynamics and inflammatory mediators.

The objective of this study was to evaluate the effect of flow rate, negative pressure, and duration on modified ultrafiltration (MUF). Eighty children weighing less than 10 kg with congenital heart disease were randomly divided into four groups: group C (conventional MUF); group H (high flow rate MUF); group P (high negative pressure MUF); and group L (long duration, high flow rate MUF). The changes in body weight, hematocrit, and hemodynamics were recorded. Tumor necrosis factor and interleukin-6 were measured before bypass, bypass stop, and MUF cessation. The durations of MUF in groups H and P were significantly shorter than in the other two groups; the volume filtered in group L was much greater than in the other three groups. The changes of bodyweight, heart rate, blood pressure, and hematocrit were similar in all groups. The increased extent of inflammatory mediators was a little lower in group L. Modified ultrafiltration can reverse hemodilution and improve cardiac function even with high flow rate or negative pressure. Prolonging the duration of MUF can filter out more inflammatory mediators, but the increased trend cannot be reversed in 15 minutes.

Seven years' experience of pediatric cardiopulmonary bypass: 8685 cases in Shanghai Children's Medical Center.

Herein we report our 7-year-experience of cardiopulmonary bypass. Between April 1, 1999, and December 31, 2005, 8685 children with congenital heart disease underwent cardiopulmonary bypass in Shanghai Children's Medical Center. The number of cases increased every year, and the number of patients younger than 1 year or weighing <10 kg also increased. Different bypass technology was adapted according to the operation. Roller pumps were used for most of the patients. Crystal cardioplegia (St. Thomas II) was used until blood cardioplegia was introduced in 2003. Ultrafiltrators were set up for infants weighing <10 kg. Mortality rates per year ranged from 1.81% to 3.70%. The most frequent complication was low cardiac output, about 12% in recent years. Arrhythmia, infection, and lung complications were the next three most frequent problems after surgery.

Left ventricular assist device for pediatric postcardiotomy cardiac failure.

Children with postcardiotomy heart failure who are unresponsive to maximal medical management have limited options for survival. Short-term mechanical circulatory support, such as with extracorporeal membrane oxygenation and ventricular assist devices, has assumed an expanding role in the care of these patients by providing a bridge to recovery. This report describes the use of left ventricular assist devices with centrifugal pumps in five pediatric cases (weight <15 kg) with postcardiotomy heart failure.

[The role of graded reoxygenation with cardiopulmonary bypass in prevention of reoxygenation injury and its safety].

OBJECTIVE: To investigate the role of graded reoxygenation with cardiopulmonary bypass (CPB) in prevention of reoxygenation injury in children with cyanotic congenital heart defects, and to evaluate its safety. METHODS: Twenty pediatric patients with cyanotic congenital heart defect were randomly divided into 2 equal groups according to CPB methodology: group 1, undergoing routine hyperoxic CPB with CPB primed and initiated at FiO(2) of 1.0, and group 2 undergoing modified CPB with graded reoxygenation (CPB was primed and initiated at FiO(2) of 0.21 and the FiO(2) was increased slowly to 30% to 60% over the next 5 to 10 minutes). Serum troponin 1 (TnI), S100beta protein, and malondialdehyde (MDA) were measured before CPB, and 1 minute, 5 minutes, and 10 minutes after CPB. Near infrared spectroscopy (NIRS) was applied to evaluate the cerebral oxygenated hemoglobin (HbO(2)) and oxidized cytochrome aa3 (CytOx) and jugular venous lactate was measured during the reoxygenation period. Clinical indexes were observed. RESULTS: Before CPB the TnI, S100beta, and MDA levels in these 2 groups were all normal without significant differences between them. After initiation of CPB the TnI, S100beta, and MDA levels in the two groups began to increase. The serum Tn1 levels 1 minute and 5 minutes after the initiation of CPB of the group 1 were significant lower than those of the group 2 (both P < 0.01). The serum S100beta levels 1, 5, and 10 minutes after the initiation of CPB in the group 2 were all lower than those of the group 1 and there were significant differences between these 2 group 5 and 10 minutes after (both P < 0.05). The serum MDA levels 1, 5, and 10 minutes of the group 1 were significantly higher than those of the group 2 (all P < 0.05). NIRS showed that HbO(2) decreased slightly because of hemodilution when CPB was begun, and then increased rapidly 2 minutes after the initiation of CPB; and CytOx decreased progressively during the reoxygenation period, however, without significant difference between these 2 groups. The serum lactate level was markedly increased 1 minute after the initiation of CPB and then gradually decreased through the reoxygenation period, however, without significant differences between these 2 groups. There was no significant difference between the two groups in clinical observation. CONCLUSION: Not damaging the cerebral aerobic metabolism, graded reoxygenation with CPB can reduce the extent of reoxygenation injury of routine hyperoxic CPB and is an easy, effective, and safe CPB strategy.

[Investigation on Qiantang medical school].

Qiantang medical school came into being during the late Ming Dynasty and the early Qing Dynasty, and lasted for 200 years until Guang Xu Reign in the late Qing Dynasty. Lu Zhiyi and Zhang Suichen were the early representative figures; Zhang Zhicong, Zhang Xiju and Gao Shizong were the mid-period representative figures; and Zhong Xuelu was the late representative figure. They respected consistently the classics and the ancients, cultivated new talents, studied medical literature with a trinity of teaching, studying the classics and practising medicine as its characteristic. Eventually, it developed under the specific background of time and geographical environment as the only academic medical school enbodying teaching, studying the classics, and medical practice as a whole with distinguished achievements.

[Arterial switch operation for transposition of the great arteries and double outlet of right ventricle with subpulmonary ventricular septum defect].

OBJECTIVE: To summarize the clinical experience on the arterial switch operation for complex congenital heart disease in recent 3 years in our hospital. METHODS: Sixty-one patients with complex congenital heart disease received by arterial switch operation from Jan 2000 to May 2003. There were 26 patients with transposition of the great arteries and intact ventricular septum (TGA-IVS), 19 patients with transposition of the great arteries and ventricular septal defect (TGA-VSD) and 16 patients with double outlet of right ventricle with subpulmonary ventricular septum defect (Taussig-Bing). RESULTS: There were 2 death among TGA-IVS cases, 4 deaths among TGA-VSD cases and 4 deaths among Taussig-Bing cases. The total operative mortality was 16%. All patients were followed up 3 months to 3 years. In all patients, the cyanosis disappeared and the physical activities increased obviously. One patients with Taussig-Bing keep moderate mitral valve reflux unchanged, 2 patients with TGA had pulmonary and aortic supra-valve stenosis with 40 mmHg pressure gradient. One patient with subpulmonary stenosis and residual VSD was re-operated 3 months later. All survivor had good heart function. CONCLUSIONS: The effect of arterial switch operation on the treatment of TGA was well accepted in this study. The procedure used in treatment on Taussig-Bing can prevent pulmonary obstruction and avoid the complication of left ventricular outflow tract obstruction caused by intraventricular repair.