Value of brain tissue oxygen saturation in neonatal respiratory distress syndrome: a clinical study.

Neonatal respiratory distress syndrome (NRDS) is one of the major causes of pre-term mortality and morbidity among very-low-birth-weight infants (VLBWI) in low- and middle-income countries (LMIC). Some of the neonates pass away despite admission and care in intensive care units (ICUs). The present clinical trial seeks the application value of elevating oxygen saturation in the brain cells of pre-term neonates born with NRDS. Near-infrared spectroscopy (NIRS) was used to monitor the neonates' microscopic cerebral oxygenation levels do determine hemoglobin concentration in brain tissues, whereas the pulse oximetry was used to measure oxygenation levels among the patients. In statistical analyses, the Analysis of Variance (ANOVA), and descriptive statistics was deployed in the Jupyter Notebook environment using Python language. High saturation of oxygen in the brain tissues result in important biological and physiological processes, including enhanced oxygen supply to cells, reduced severity of NRDS, and balancing oxygen demand and supply. The correlations of oxygen saturation with systemic saturation of oxygen, the saturation of oxygen in brain tissues, the association between brain-specific and systemic saturation, and the impact of these outcomes on clinical practices were deliberated. Also, the pH gas values, the saturation of oxygen in neonates' brain tissues, metabolic acidosis, the effect of acid-base balance and cerebral oxygen supply, and the oxygenation of brain tissues and the pH values emerged as important variables of oxygenation of brain tissues in pre-term neonates. Oxygen saturation in brain cells influence vital physiological and biological processes. Balancing acid-base saturation or levels is needed despite the challenging achievement. Oxygenation of brain tissues improve the brain's overall functioning.

Blocking brown adipocyte ß3-adrenoceptor attenuates blood-spinal cord barrier impairment and chronic postsurgical pain in a rat model of preoperative stress.

Preoperative stress has been recognized as an independent risk factor for chronic postsurgical pain (CPSP). However, the underlying mechanisms of CPSP influenced by preoperative stress remain elusive. Previous studies indicated that excessive stress could induce disruption of the blood-spinal cord barrier (BSCB). We wondered whether and how BSCB involves in CPSP by using a single prolonged stress (SPS) combining plantar incision model in male rats to mimic preoperative stress-related postsurgical pain. Here, we observed that preoperative SPS-exposed rats exhibited relentless incisional pain, which was accompanied by impairment of BSCB and persistent elevation of serum IL-6. Intraperitoneal injections of Tocilizumab (an IL-6 receptor monoclonal antibody) not only mitigated BSCB breakdown but also alleviated pain behaviors. In addition, intervening ß3-adrenoceptor (ADRB3) signaling in brown adipocytes by SR59230a (a specific ADRB3 antagonist) treatment or removal of brown adipose tissues could effectively decrease serum IL-6 levels, ameliorate BSCB disruption, and alleviate incisional pain. Further results displayed that SI-exposed rats also showed markedly spinal microglia activation. And exogenous His-tagged IL-6 could pass through the disrupted BSCB, which might contribute to microglia activation. Injection of SR59230a or ablation of brown adipose tissues could effectively reduce the activation of spinal microglia. Thus, our findings suggest that serum IL-6 induced by brown adipocyte ADRB3 signaling contributed to BSCB disruption and spinal microglia activation, which might be involved in preoperative stress mediated CPSP. This work indicates a promising treatment strategy for preoperative stress induced CPSP by blocking ADRB3.

Construction of Z-scheme AgCl/BiOCl heterojunction with oxygen vacancies for improved pollutant degradation and bacterial inactivation.

A facile Z-scheme AgCl/BiOCl heterojunction photocatalyst with oxygen vacancies was fabricated by a water-bath method. The structural, morphological, optical and electronic properties of as-synthesized samples were systematically characterized. The oxygen vacancies were confirmed by EPR, which could optimize the band-gap of the AgCl/BiOCl heterojunction and improve the photo-induced electron transfer. The optimized AgCl/BiOCl heterojunction showed excellent photocatalytic degradation efficiency (82%) for tetracycline (TC). Simultaneously, E. coli was completely inactivated within 60 min due to the AgCl/BiOCl heterojunction. The elevated catalytic activity of the optimal AgCl/BiOCl heterojunction was ascribed to the synergistic effect of the enhanced light absorption and effective photoinduced charge carrier separation and transfer. Moreover, the degradation efficiency of the AgCl/BiOCl heterojunction towards ofloxacin, norfloxacin and Lanasol Red 5B was 73%, 74% and 96%, respectively. The experimental factors for the degradation efficiency of TC were also studied. Furthermore, active species trapping experiments indicated that superoxide radicals (˙O2-) were the main reactive species, and the Z-scheme charge transfer mechanism helped to improve the photocatalytic activity.

Least-squares method constrained by phase smoothness for correcting illumination fluctuation errors in phase-shifting profilometry.

Phase-shifting fringe projection profilometry usually suffers from the errors induced by illumination fluctuations. As a result, ripple-like artifacts that have the same periods as fringes appear on the phase map. Because the illumination fluctuations cannot be simply modeled using fringe harmonics, their induced errors are difficult to remove by use of a phase-shifting algorithm. To solve this problem, this paper suggests a least-squares method constrained by phase smoothness. This method calculates fringe phases using the generalized phase-shifting algorithm and estimates coefficients related to illumination fluctuation by use of smoothed phase map. Alternately implementing these two steps enables one to eliminate effects of illumination fluctuations on the measurement results. Experimental results demonstrate that this proposed algorithm is helpful for improving measurement accuracy.

Least-squares method constrained by phase smoothness for correcting illumination fluctuation errors in phase-shifting profilometry: erratum.

This erratum corrects an error in Eq. (29) of the original paper, Appl. Opt.62, 8451 (2023)APOPAI0003-693510.1364/AO.505327.

Quasi-pointwise two-step phase-shifting profilometry with the fringe parameters estimated statistically.

Fringe projection profilometry is a popularly used three-dimensional measurement technique in which phase-measuring algorithms based on two-step phase shifting are usually used because of their best tradeoff between measurement resolution and speed. Most two-step phase-shifting algorithms involve neighboring or other spatial operations, thus having degraded accuracies at edges and discontinuities of the measured object surface. Pointwise two-step algorithms enable overcoming this issue. With them, however, the offsets of the dynamic ranges of the projector and camera are usually improperly overlooked or inaccurately estimated, thus inducing errors in their measurement results. For solving this problem, this paper suggests a quasi-pointwise two-step phase-shifting algorithm for fringe projection profilometry. This algorithm models the captured fringe patterns practically by taking the offsets of the dynamic ranges of the projector and camera into account, and estimates the fringe parameters from the statistics of fringe intensities. As a result, we can calculate fringe phases in a pointwise way from two fringe patterns having a phase difference of π/2 radians. The simulation and experimental results demonstrate that the proposed method has a relatively low level of errors in measuring object surfaces having isolated regions and discontinuities.

Harmonics elimination in phase-shifting fringe projection profilometry by use of a non-filtering algorithm in frequency domain.

In phase-shifting fringe projection profilometry, fringe harmonics caused by device nonlinearities as well as other factors may badly ruin the measurement results. Generally, the used phase-shifting algorithm enables restraint of effects of harmonics below a certain order depending on the number of phase shifts. When reducing the number of phase shifts for efficiency, high order harmonics will affect the phase-measuring results because of aliasing caused by insufficient sampling rate. To overcome this issue, this paper suggests a non-filtering technique operating in frequency domain, that enables improvement of measurement accuracy by eliminating effects of high order harmonics. With this technique, the phase-shifting algorithm is restated as a process of retrieving the fundamental complex fringes from the phase-shifted fringe patterns. Implementing a Fourier transform to this calculated complex fringe pattern, the actual fundamental signals and the aliased harmonics have their own lobes with separated peaks in the frequency domain. We reconstruct each order of the aliased harmonics by exploiting their relations with the fundamental signals and then estimate their magnitudes by using the spectral peaks. Instead of directly filtering the fringe spectrum, we subtract spectra of the harmonics from Fourier transform of the just calculated complex fringes, so that the Fourier spectrum of the fundamental fringes without harmonics is recovered through an iterative operation. Further, the phase map is measured accurately. Simulation and experimental results confirm that this proposed method can significantly suppress effects of fringe harmonics. Meanwhile, by taking advantage of non-filtering, it effectively preserves the edges and details of the measured surfaces from being blurred.

Joint least-squares algorithm correcting phase-shift errors and detector nonlinearity simultaneously in phase-shifting interferometry.

Phase-shifting interferometry may suffer from the errors caused by the miscalibration of the phase shifter and the nonlinearity of the detector simultaneously. These errors are not easy to eliminate because they are generally coupled with each other in interferograms. For solving this issue, we suggest a joint least-squares phase-shifting algorithm. It allows one to decouple these errors through an alternate least-squares fitting procedure, thus accurately estimating phases, phase shifts, and coefficients of the detector response simultaneously. The converging condition of this algorithm, associated with the uniqueness of the equation solution and anti-aliasing phase shifting, is discussed. Experimental results demonstrate that this proposed algorithm is helpful for improving phase-measuring accuracy in phase-shifting interferometry.

Construction of a novel two-dimensional AgBiO3/BiOBr step-scheme heterojunction for enhanced broad-spectrum photocatalytic performance.

Efficient S-scheme heterojunction photocatalysts were prepared through in situ growth of AgBiO3 on BiOBr. The self-assembled hierarchical structure of AgBiO3/BiOBr was formed from flower-like AgBiO3 and plate-like BiOBr. The optimized AgBiO3/BiOBr heterojunction possessed excellent visible-light photocatalytic degradation efficiency (83%) for ciprofloxacin (CIP) after 120 min, with 1.46- and 4.15-times higher activity than pure AgBiO3 and BiOBr, respectively. Furthermore, the removal ratio of multiple organic pollutants including tetracycline, Rhodamine B, Lanasol Red 5B and methyl orange was also investigated. Environmental interference experiments demonstrated that high pollutant concentrations, low photocatalyst dose and the addition of ions (SO4 2-, PO4 3-, HPO4 2-, H2PO4 -) inhibited the photocatalytic activities. Subsequently, a simultaneous degradation experiment showed the competitive actions between CIP and RhB for radicals, decreasing the photocatalytic activity of CIP. Furthermore, trapping and electron spin resonance experiments showed that h+ and ˙O2 - played a certain role in the degradation process and that ˙OH acted as assistant.

Mechanism of simultaneous lead and chromium removal from contaminated wastewater by a schwertmannite-like mineral.

In this study, a schwertmannite-like mineral was synthesized for the removal of lead (Pb) and chromium (Cr) from contaminated wastewater. A shaking flask test was performed (150 r/min, 1 h) with FeSO4·7H2O, H2O2, Na2SiO3, and CaCl2 added for the mineral synthesis reaction. Results show that optimal performance was achieved with the addition of 1.24 g/L FeSO4·7H2O, 0.75 g/L H2O2, 1.27 g/L Na2SiO3, and 0.44 g/L CaCl2 at a water temperature of 28 °C, with coexisting ion (Na+, K+, Mg2+) concentrations of 1.50 mmol/L and 0.50 mmol/L EDTA as a complexing agent. Under these optimal conditions, maximum Pb and Cr removal rates of 95.08% and 97.99%, respectively, were achieved within the first 1 min of the mineral synthesis reaction, with the synthesis reaction completed by 6 min. The simultaneous removal of Pb and Cr during the schwertmannite-like material synthesis process occurred via electrostatic adsorption and coprecipitation. When the concentration of the complexing agent was increased from 0.75 to 6.03 mmol/L, the Pb removal rate decreased from 71.88 to 35.45%, and the Cr removal rate decreased from 95.13 to 75.07%, showing that Pb and Cr removal exhibited significant levels of inhibition. In contrast, varying reaction temperatures induced no significant differences. The Pb and Cr dissolution rates from Pb/Cr-containing schwertmannite-like minerals were 8.18% and 2.86% after 40 days, respectively. Therefore, the risk of secondary dissolution of heavy metals is small.

The Arabidopsis Mitochondrial Pseudouridine Synthase Homolog FCS1 Plays Critical Roles in Plant Development.

As the most abundant RNA modification, pseudouridylation has been shown to play critical roles in Escherichia coli, yeast and humans. However, its function in plants is still unclear. Here, we characterized leaf curly and small 1 (FCS1), which encodes a pseudouridine synthase in Arabidopsis. fcs1 mutants exhibited severe defects in plant growth, such as delayed development and reduced fertility, and were significantly smaller than the wild type at different developmental stages. FCS1 protein is localized in the mitochondrion. The absence of FCS1 significantly reduces pseudouridylation of mitochondrial 26S ribosomal RNA (rRNA) at the U1692 site, which sits in the peptidyl transferase center. This affection of mitochondrial 26S rRNA may lead to the disruption of mitochondrial translation in the fcs1-1 mutant, causing high accumulation of transcripts but low production of proteins. Dysfunctional mitochondria with abnormal structures were also observed in the fcs1-1 mutant. Overall, our results suggest that FCS1-mediated pseudouridylation of mitochondrial 26S rRNA is required for mitochondrial translation, which is critical for maintaining mitochondrial function and plant development.

Anti-aliasing phase reconstruction via a non-uniform phase-shifting technique.

The conventional phase-shifting techniques commonly suffer from frequency aliasing because of their number of phase shifts below the critical sampling rate. As a result, fringe harmonics induce ripple-like artifacts in their reconstructed phase maps. For solving this issue, this paper presents an anti-aliasing phase-measuring technique. Theoretical analysis shows that, with phase-shifting, the harmonics aliased with the fundamental frequency component of a fringe signal depend on the greatest common divisor (GCD) of the used phase shifts. This fact implies a possibility of removing such aliasing effects by selecting non-uniform phase shifts that together with 2π have no common divisors. However, even if we do so, it remains challenging to separate harmonics from the fundamental fringe signals, because the systems of equations available from the captured fringe patterns are generally under-determined, especially when the number of phase shifts is very few. To overcome this difficulty, we practically presume that all the points over the fringe patterns have an identical characteristic of harmonics. Under this constraint, using an alternate iterative least-squares fitting procedure allows us to estimate the fringe phases and the harmonic coefficients accurately. Simulation and experimental results demonstrate that this proposed method enables separating high order harmonics from as few as 4 fringe patterns having non-uniform phase shifts, thus significantly suppressing the ripple-like phase errors caused by the frequency aliasing.

Removal of Antimony(V) from Drinking Water Using nZVI/AC: Optimization of Batch and Fix Bed Conditions.

Antimony (Sb) traces in water pose a serious threat to human health due to their negative effects. In this work, nanoscale zero-valent iron (Fe0) supported on activated carbon (nZVI) was employed for eliminating Sb(V) from the drinking water. To better understand the overall process, the effects of several experimental variables, including pH, dissolved oxygen (DO), coexisting ions, and adsorption kinetics on the removal of Sb(V) from the SW were investigated by employing fixed-bed column runs or batch-adsorption methods. A pH of 4.5 and 72 h of equilibrium time were found to be the ideal conditions for drinking water. The presence of phosphate (PO43-), silicate (SiO42-), chromate (CrO42-) and arsenate (AsO43-) significantly decreased the rate of Sb(V) removal, while humic acid and other anions exhibited a negligible effect. The capacity for Sb(V) uptake decreased from 6.665 to 2.433 mg when the flow rate was increased from 5 to 10 mL·min-1. The dynamic adsorption penetration curves of Sb(V) were 116.4% and 144.1% with the weak magnetic field (WMF) in fixed-bed column runs. Considering the removal rate of Sb(V), reusability, operability, no release of Sb(V) after being incorporated into the iron (hydr)oxides structure, it can be concluded that WMF coupled with ZVI would be an effective Sb(V) immobilization technology for drinking water.

Adsorption of Sb(III) from Aqueous Solution by nZVI/AC: A Magnetic Fixed-Bed Column Study.

The effectiveness of nanoscale zero-valent iron(nZVI) immobilized on activated carbon (nZVI/AC) in removing antimonite (Sb(III)) from simulated contaminated water was investigated with and without a magnetic fix-bed column reactor. The experiments were all conducted in fixed-bed columns. A weak magnetic field (WMF) was proposed to increase the exclusion of paramagnetic Sb(III) ions by nZVI/AC. The Sb(III) adsorption to the nZVI and AC surfaces, as well as the transformation of Sb(III) to Sb(V) by them, were both increased by using a WMF in nZVI/AC. The increased sequestration of Sb(III) by nZVI/AC in the presence of WMF was followed by faster nZVI corrosion and dissolution. Experiments were conducted as a function of the pH of the feed solution (pH 5.0-9.0), liquid flow rate (5-15 mL·min-1), starting Sb(III) concentration (0.5-1.5 mg·L-1), bed height nZVI/AC (10-40 cm), and starting Sb(III) concentration (0.5-1.5 mg·L-1). By analyzing the breakthrough curves generated by different flow rates, different pH values, different inlet Sb(III) concentrations, and different bed heights, the adsorbed amounts, equilibrium nZVI uptakes, and total Sb(III) removal percentage were calculated in relation to effluent volumes. At pH 5.0, the longest nZVI breakthrough time and maximal Sb(III) adsorption were achieved. The findings revealed that the column performed effectively at the lowest flow rate. With increasing bed height, column bed capacity and exhaustion time increased as well. Increasing the Sb(III) initial concentration from 0.5 to 1.5 mg·L-1 resulted in the rise of adsorption bed capacity from 3.45 to 6.33 mg·g-1.

Spatial phase-shifting profilometry by use of polarization for measuring 3D shapes of metal objects.

In this paper, we present a polarization spatial phase-shifting method for fringe projection profilometry. It enables us to measure the three-dimensional shape of a metal object in a fast way requiring only a single-shot implementation. With this method, a couple of projectors are equipped, in front of their lens, with linear polarization filters having orthogonal polarization directions, so that they can simultaneously cast two sinusoidal fringe patterns having different phase shifts onto the measured metal surfaces without mixture. To register the two projected patterns, we suggest a fringe alignment method based on the epipolar geometry between the projectors. By taking advantage of the property of metal surfaces in maintaining polarization state of incident light, the deformed fringe patterns on the measured surfaces are captured by using two coaxially-arranged polarization cameras. As a result, the fringe phases are calculated by using a two-step phase-shifting algorithm and further the 3D shapes of the measured surfaces are reconstructed. Experimental results demonstrate the proposed method to be valid and efficient in measuring metal objects.

Adsorption Kinetics of Arsenic (V) on Nanoscale Zero-Valent Iron Supported by Activated Carbon.

The presence of arsenic (As) in drinking water is of serious concern due to its negative impact on human health. This work reports on the kinetics of nanoscale zero-valent iron (Fe0) supported by activated carbon (NZVI/AC) for the removal of As (V) species from aqueous solutions. To better understand the factors affecting this process, we investigated the effects of various experimental parameters including initial As (V) concentration, adsorbent dosage, pH, temperature, and coexisting ions on the adsorption kinetics using a batch-adsorption method. The optimum conditions for As (V) removal by NZVI/AC were found to be: 318 K, pH 3.5, an adsorbent dosage of 1.5 g/L, and an equilibrium time of 72 h. A greater mass of NZVI/AC, lower concentration of As (V) and lower pH positively promoted adsorption kinetics. The presence of phosphate (PO43-) and silicate (SiO42-) markedly inhibited As (V) removal kinetics. However, in the presence of 4.5 g/L NZVI/AC, ≥99.9% of As (V) was removed from raw groundwater.

Efficient depth recovering method free from projector errors by use of pixel cross-ratio invariance in fringe projection profilometry.

In fringe projection profilometry, errors related to projectors are not easy to compensate for, as a projector is much more difficult to calibrate than a camera. Immune to projector errors, the depth recovering method based on pixel cross-ratio invariance enables circumventing this issue by calculating the depth of a point from the shift of its camera pixel instead of from its fringe phase. With this existing technique, however, one has to search three reference phase maps along epipolar lines for pixels having the same phases. Doing so increases the measurement time significantly. To improve measurement efficiency, this paper derives, from the pixel cross-ratio invariance, a generic function representing the relationship between the depths and the corresponding pixel shifts and suggests a calibration method for determining its coefficient matrices. Using this function allows us to recover object depths just by searching a single reference phase map, thus reducing the time duration for data processing to about one-third. Besides, different from the previous method, which depends on exactly three reference phase maps, the proposed method calculates the function coefficients from more reference phase maps in the least-squares sense and denoises the benchmark reference phases by use of averaging technique, thus improving measurement accuracy. Experimental results demonstrate this method to be effective in improving measurement accuracy and efficiency.

Transcriptome-wide analysis of pseudouridylation of mRNA and non-coding RNAs in Arabidopsis.

Pseudouridine (Ψ) is widely distributed in mRNA and various non-coding RNAs in yeast and mammals, and the specificity of its distribution has been determined. However, knowledge about Ψs in the RNAs of plants, particularly in mRNA, is lacking. In this study, we performed genome-wide pseudouridine-sequencing in Arabidopsis and for the first time identified hundreds of Ψ sites in mRNA and multiple Ψ sites in non-coding RNAs. Many predicted and novel Ψ sites in rRNA and tRNA were detected. mRNA was extensively pseudouridylated, but with Ψs being under-represented in 3'-untranslated regions and enriched at position 1 of triple codons. The phenylalanine codon UUC was the most frequently pseudouridylated site. Some Ψs present in chloroplast 23S, 16S, and 4.5S rRNAs in wild-type Col-0 were absent in plants with a mutation of SVR1 (Suppressor of variegation 1), a chloroplast pseudouridine synthase gene. Many plastid ribosomal proteins and photosynthesis-related proteins were significantly reduced in svr1 relative to the wild-type, indicating the roles of SVR1 in chloroplast protein biosynthesis in Arabidopsis. Our results provide new insights into the occurrence of pseudouridine in Arabidopsis RNAs and the biological functions of SVR1, and will pave the way for further exploiting the mechanisms underlying Ψ modifications in controlling gene expression and protein biosynthesis in plants.

A membrane-anchored fluorescent probe for detecting K(+) in the cell microenvironment.

Cell-surface fluorescent probes are effective tools in cell biology and engineering. Here, we for the first time report a diacyllipid-aptamer conjugate-based fluorescent probe which could anchor on cell membrane for real-time tracking of potassium ions in the cell microenvironment.

Nucleic acid aptamer-mediated drug delivery for targeted cancer therapy.

Aptamers are emerging as promising therapeutic agents and recognition elements. In particular, cell-SELEX (systematic evolution of ligands by exponential enrichment) allows in vitro selection of aptamers selective to whole cells without prior knowledge of the molecular signatures on the cell surface. The advantage of aptamers is their high affinitiy and binding specificity towards the target. This Minireview focuses on single-stranded (ss) oligonucleotide (DNA or RNA)-based aptamers as cancer therapeutics/theranostics. Specifically, aptamer-nanomaterial conjugates, aptamer-drug conjugates, targeted phototherapy and targeted biotherapy are covered in detail. In reviewing the literature, the potential of aptamers as delivery systems for therapeutic and imaging applications in cancer is clear, however, major challenges remain to be resolved, such as the poorly understood pharmacokinetics, toxicity and off-target effects, before they can be fully exploited in a clinical setting.