Prognostic value of systemic immune-inflammation index in patients with urinary system cancers: a meta-analysis.

OBJECTIVE: The systemic immune-inflammation index (SII), an inexpensive and widely available hematologic marker of inflammation, has been linked to tumor progression, metastatic spread, and poor patient prognosis. The objective of this study is to explore the prognostic value of SII in patients with urinary system cancers (USCs). MATERIALS AND METHODS: A comprehensive literature search was conducted by searching the PubMed, EMBASE, Web of Science, Cochrane Library, Chinese National Knowledge Infrastructure (CNKI), and Wanfang databases from inception to May 10, 2020, to identify potential studies that assessed the prognostic role of the SII in USCs. The hazard ratio (HR) with a 95% confidence interval (CI) were used to evaluate the correlation between SII and overall survival (OS), progression-free survival (PFS), and cancer-specific survival (CSS) in USCs patients. RESULTS: A total of 12 studies, including 2,693 USCs patients, were eventually included in the meta-analysis. Elevated SII index was significantly associated with poor OS (HR=1.28, 95% CI: 1.17-1.39, p<0.001), PFS (HR=1.51, 95% CI: 1.25-1.82, p<0.001) and CSS (HR=3.42, 95% CI: 1.49-7.91, p<0.001). Furthermore, subgroup analysis indicated that higher SII than a cutoff value could predict poor OS in renal cell carcinoma (HR=1.23, p<0.001), prostate carcinoma (HR=1.95, p<0.001), bladder carcinoma (HR=5.40, p<0.001), testicular cancer (HR=6.09, p<0.001) and upper tract urothelial carcinoma (HR=2.19, p<0.001). Besides, these associations did not vary significantly by tumor subtypes and stages of USCs, sample sizes, study types, cutoff value defining elevated NLR, treatment methods, and NOS scores. CONCLUSIONS: SII may serve as a useful prognostic indicator in USCs and contribute to prognosis evaluation and treatment strategy formulation. However, more well-designed studies are warranted to verify our findings.

[The expression and significance of heparanase in oral squamous cell carcinoma with different metastatic potentials].

Objective: To detect the expression of heparanase (HPSE) in oral squamous cell carcinoma (OSCC) with different metastatic potentials and investigate its clinical significance. Methods: Transcriptional and translational status of HPSE in OSCC cell lines with different metastatic capacities, primary OSCC samples and their paired metastatic cancer tissues were determined using real-time polymerase chain reaction (PCR) and Western blotting analysis. Immunohistochemistry was used to evaluate HPSE expression in 131 OSCC samples. The correlation between HPSE expression pattern and clinicopathological parameters and clinical outcome in patients with OSCC were analyzed. HPSE level was reduced using HPSE-siRNAs in OSCC cell lines and its impact on cell migration and invasion was measured by scratch assay and matrigel invasion assay. Results: The mRNA and protein levels of HPSE were remarkably up-regulated in OSCC cell lines with highly metastatic capacity (P<0.000 1) and metastatic OSCC tissues (P<0.000 1). The protein levels of HPSE were strongly associated with lymph node metastasis (P<0.000 1) and tumor node metastasis stage (P=0.012). Survival analyses revealed that high HPSE expression was associated with worse overall survival (P=0.000 3). Multivariate Cox proportional analyses indicated that HPSE expression was strongly associated with clinical outcome in patients with OSCC (HR=2.203, 95% CI: 1.203-3.988, P=0.009). The siRNA-mediated silencing of HPSE could suppress the migration and invasion (P=0.008) of HN12 cells in vitro. Conclusions: The up-regulation of HPSE contributes to invasion and metastasis of OSCC. HPSE may serve as a useful biomarker for patients with OSCC.

Genetic diversity based on SSR analysis of the cultured snakehead fish, Channa argus, (Channidae) in China.

The snakehead fish Channa argus is an important food fish in China. We identified six microsatellite loci for C. argus. These six microsatellite loci and four other microsatellite markers were used to analyze genetic diversity in four cultured populations of C. argus (SD, JX, HN, and ZJ) and determine their relationships. A total of 154 alleles were detected at the 10 microsatellite loci. The average expected and observed heterozygosities varied from 0.70-0.84 and 0.69-0.83, respectively, and polymorphism information content ranged between 0.66 and 0.82 in the four populations, indicating high genetic diversity. Population JX deviated from mutation-drift equilibrium and may have experienced a recent bottleneck. Analysis of pairwise genetic differentiation revealed FST values that ranged from 0.028 to 0.100, which indicates a moderate level of genetic differentiation. The largest distances were observed between populations HN and SD, whereas the smallest distances were obtained between populations HN and JX. Genetic clustering analysis demonstrated that the ZJ and HN populations probably share the same origin. This information about the genetic diversity within each of the four populations, and their genetic relationships will be useful for future genetic improvement of C. argus through selective breeding.

Effects of high-mobility group box 1 on the proliferation and odontoblastic differentiation of human dental pulp cells.

AIM: To investigate the expression of high-mobility group box 1 (HMGB1) in human dental pulp tissues and the effects of HMGB1 on proliferation and odontoblastic differentiation of human dental pulp cells (hDPCs). METHODOLOGY: Immunohistochemical assay, immunofluorescence staining and flow cytometric analysis were used to detect the expression of HMGB1 in the human dental pulp and hDPCs, respectively. The proliferation of hDPCs was examined by CCK-8 after culturing human primary hDPCs in the presence of HMGB1 with different doses. Odontoblastic differentiation of hDPCs was determined using alkaline phosphatase (ALPase) activity assay and mineralized nodule formation. Important mineralization-related genes such as ALP, dental sialophosphoprotein (DSPP) and dental matrix protein-1 (DMP-1) were determined by real-time polymerase chain reaction. Western blot analysis was performed to determine the difference in expressions of DMP-1 and DSP with or without the presence of exogenous HMGB1. Simultaneously, messenger RNA and protein levels of HMGB1 and RAGE were also detected. The protein level of HMGB1 in the supernatants was quantified using ELISA analysis. RESULTS: HMGB1 was found in human dental pulp tissue and in the nuclei of hDPCs. During hDPC odontoblastic differentiation, HMGB1 translocated from the nuclei to the cytoplasm and then secreted out from hDPCs. Exogenous HMGB1 promoted hDPC proliferation and mineralized nodule formation. It up-regulated the activity of ALPase and the mRNA and protein levels of dentine matrix protein-1 (DMP-1), alkaline phosphatase (ALP), dentine sialophosphoprotein (DSPP) and receptor for advance glycation end (RAGE) of hDPCs. CONCLUSION: HMGB1 promoted the proliferation and odontoblastic differentiation of hDPCs.

Copper(II) complexes with N,N'-dialkyl-1,10-phenanthroline-2,9-dimethanamine: synthesis, characterization, DNA-binding thermodynamical and kinetic studies.

Copper(II) complexes (Cu-L, L=N,N'-dialkyl-1,10-phenanthroline-2,9-dimethanamine) were synthesized and characterized by elemental analyses, IR spectra and conductance measurement. The interaction of the copper(II) complex with calf thymus DNA was studied by means of UV melting experiments, fluorescence spectra and circular dichroic spectra. Using ethidium bromide as a fluorescence probe, the binding mode of the complexes Cu-L with calf-thymus DNA was studied spectroscopically. The results indicate that the complexes Cu-L perhaps interact with calf-thymus DNA by both intercalative and covalent binding. Kinetics of binding of the cupric complexes to DNA was studied for the first time using ethidium bromide as a fluorescence probe with stopped-flow spectrophotometer under pseudo-first-order condition. The stronger binding of two steps in the process of the complexes Cu-L interacting with DNA was observed, and the probable interaction process was discussed in detail. The corresponding k(obs) and E(a) of binding to DNA (where k(obs) is the observed pseudo-first-order rate constant, E(a) is the observed energy of activation) were obtained.

[A survey of the changes of serum inorganic salts and alkaline phosphatase after jaw fractures].

OBJECTIVE: To study the changes of serum salts and alkaline phosphatase in patients with jaw fractures and its roles. METHODS: The serum salts and alkaline phosphatase were determined in 40 cases of jaw fracture and 14 health persons. RESULTS: The results showed that there was no significant difference in Na(+), K(+), Cl(-), Ca(2+) contents between jaw fracture group and normal group (P>0.05), but P(3-) Mg(2+), ALP levels were higher in jaw fracture group than that in normal group (P<0.05). Furthermore the Ca(2+), P(3-), Mg(2+), ALP were higher in multiple or communition fracture of jaw than in single or double line fracture (P<0.05) except Ca content. The level of Ca(2+) was higher in single jaw fracture than in jaw damage with whole body injury (P>0.05) but the levels of P(3-),Mg(2+), ALP were no difference (P>0.05). As the same all the Ca(2+), P(3-), Mg(2+), ALP levels were higher in early stage of fracture than in 4 weeks after fracture (P<0.05). CONCLUSION: The contents of Ca(2+) P(3-), Mg(2+) ALP were increased after the axilla mandible were fractured and it has an important regulation role in the activity of osteoblast and osteoclast in healing of fractured jaw.

Synthesis, characterization and cytotoxicity of lanthanum(III) complexes with novel 1,10-phenanthroline-2,9-bis-alpha-amino acid conjugates.

Eight lanthanum(III) complexes with novel 1,10-phenanthroline-2,9-bis-alpha-amino acid conjugates were synthesized and characterized by elemental analyses, IR, MS, 1H-NMR, thermal analysis and conductance measurement. All lanthanum(III) complexes and the corresponding soluble ligand in water have been assayed for antitumor activity in vitro against HL-60 (human leukocytoma) cells, HCT-8 (human coloadenocarcinoma) cells, BGC-823 (human stomach carcinoma) cells, BeL-7402 (human liver carcinoma) cells and KB (human nasopharyngeal carcinoma) cells. The results show that several complexes have relative activity against different cell lines. In particular, the complexes La(L2) and La(L5) show relatively high activity against the BeL-7402 cell line. Moreover, they are slightly more effective than cisplatin. DNA binding studies indicate that the complex La(L2) possibly interacts with calf thymus DNA by both intercalative and covalent binding.

[An experimental study on dental pulp histology after the inferior alveolar nerve damage and autogenous nerve graft].

The dental pulpal histology was studied experimentally in rabbits after the inferior alveolar neurovascularity damage and nerve graft. The results revealed that the dental vitality was gradually repaired at different intervals after the inferior alveolar neurovascularity damage. In the early damaged period the pulpal blood vessels were reduced in number and some of them were swollen and permeable, in the late period the structure of the microvascular system became normal and abundant. The pulpal architecture was altered in the initial stage and with the tendency to repair gradually. The pulpal axon degenerated in early stage and reinnervated later. The authors indicate that there are no evident affections for dental pulpal tissues whatever the inferior alveolar neurovascularity is damaged and repaired. This has an important clinical value to define the dental pulp vitality in mandibular subapical osteotomy and tumor resection.

[The clinical observation of 247 tuberculosis cases in Oral Maxillofacial region]

This paper presents a report of clinical observation on tuberculosis of oral and maxillofacial region in 247 patients.Of these which diagnosed by histopathology.94 cases are from mucous membrane of oral cavity,108 subjects from lymph nodes,27 ones from parotid gland,15 persons from facial dermis and 3 from jaw.Among 247 patients.154 cases were male and 93 female.The youngest was 2 years old and the eldest one was 71 of age and the mean age was 32 years old.The shortest course of disease was 15 days and the longest was 30 years,the mean course of time was two and half years.The rate of clinical diagnosis accord with pathology was 41.3%,most error diagnosis were to suspect inflammation,tumors and ulcer.In addition,the etiology,the clinical manifestion,diagnosis and therapies of oral and maxillofacial tuberculosis were analyzed.

A comparative experimental study on primary and secondary nerve grafting following inferior alveolar nerve injury.

The damaged inferior alveolar nerve of rabbit was repaired with autogenous ipsilateral greater auricular nerve grafting primarily and secondarily, and electrophysiological, ultrastructural, microvascular and histochemical investigations were performed in this study. The results indicated that the experimental effects of group 1 out of 3 groups (group 1 with immediate grafting, group 2 with grafting after 4 weeks, and group 3 with grafting after 8 weeks) were the best and those of group 2 were second only to group 1 with no obvious difference between these two groups (P greater than 0.05). However, the results of group 3 were not good enough, and there was significant difference from the other two groups (P less than 0.05). Therefore, we were led to conclude that the injury should be repaired at once or within 4 weeks after inferior alveolar nerve was damaged, or within 2 or 3 months thereafter, to improve the function of the injured nerve as greatly as possible.