[Adsorbed subunit influenza vaccine: its isolation and characteristics]. / Adsorbirovannaia grippoznaia sub''edinichnaia (AGS)vaktsina: poluchenie i kharakteristika.

Experimental batches of adsorbed subunit influenza vaccine were prepared from the envelope of glycoprotein antigens separated from the influenza virion by treatment with a cationic detergent (cetyltrimethylammonium bromide). Purified and concentrated influenza virus strains A(H1N1) and A(H3N2) were obtained by gradient centrifugation; additional purification, with a view to removing ovalbumin and structural components of the chorioallantoic membrane, was achieved by gelfiltration. The composition and biological properties of the vaccine are described.

[Immune response of noninbred mice to subvirion influenza vaccines with various antigen and sorbent loads]. / Izuchenie immunnogo otveta neinbrednykh myshei na subvirionnye gripkoznye vaktsiny s var'iruemoi nagruzkoi antigena i sorbenta.

The variants of splitted and subunit influenza monovaccines from virus strains A/Leningrad/385/80R (H3N2) and A/Kiev/59/79R (H1N1), adsorbed on aluminium hydroxide and having the varying content of hemogglutinin and the carrier, have been studied. The immune response of noninbred mice to a single and double injections of these vaccines have been evaluated, the concentrations of the antigen and the carrier inducing a high response in the animals, have been determined. Differences in the immunological potency of hemagglutinins H1 and H3 have been noted.

[Determination of bacterial endotoxin admixtures in inactivated influenza vaccines]. / Opredelenie primesei bakterial'nykh éndotoksinov v inaktivirovannykh grippoznykh vaktsinakh.

The materials substantiating the possibility of using the method for the determination of the lethal effect of endotoxin on dactinomycin-treated mice are presented. This determination is made with a view to detecting the admixtures of endotoxins in whole-virion and subvirion inactivated influenza vaccines at different stages of their manufacture, as well as in the final product. The proposed test is highly sensitive, rather simple in its practical realization and can be used for evaluating the degree of the purification of influenza vaccines from endotoxins.