The expression of PGC-1α in carotid atherosclerotic plaque induced by cytomegalovirus / 中华实验和临床病毒学杂志

Objective@#To investigate the role of peroxisome proliferator-activated-receptor-γ coactivator-1α (PGC-1α) in carotid atherosclerosis due to human cytomegalovirus (HCMV) infection.@*Methods@#Thirty-three samples of carotid arterial sclerosis plaques after carotid endarterectomy (CEA) were collected in the experiment group, and 12 pieces of normal intracranial arteries were collected in the control group. The plaques of carotid artery were studied using in situ hybridization (ISH) and immunohistochemistry (IHC) for the role of PGC-1α in HCMV related atherosclerosis. HCMV immediate early (IE) gene of two groups was detected using ISH, and the expression of PGC-1α using IHC.@*Results@#HCMV IE gene was positive 72.7% in the atherosclerotic plague group, while 16.6% in the control group, there was significant difference between the two groups (P<0.05). As to PGC-1α expression, in the atherosclerosis group, 8/33(24.2%) were strongly positive (+ + + ), 9/33(27.2%) were positive (+ + ), 6/33(18.2%) were weakly positive (+ ), and 10/33(30.3%) were negative (-). While in the control group, 1/12(8.3%) was strongly positive (+ + + ), 2/12(16.7%) were positive (+ + ), and 1/12 (8.3%) was weakly positive (+ ), and 8/12 (66.7%) were negative (-). The differences between the two groups were statistically significant (P<0.05).@*Conclusions@#HCMV immediate early gene and PGC-1α were both related to athersclerotic plaque formation, suggesting that PGC-1α may play an important role in as the plaque formation due to HCMV infection.

Role of HCMV infection in the activation and oxidative stress of cultured human umbilical vein endothelial cells / 中华实验和临床病毒学杂志

Objective@#To investigate the role of the activation and oxidative stress of cultured human umbilical vein endothelial cells (HUVEC) after HCMV infection.@*Methods@#HUVECs were divided into four groups: control, HCMV(+ ), after HCMV AD169 infection, and the supernatant of the culture was extracted, and the cells were lysed. The levels of vascular cellular adhesion molecule-1 (VCAM-1) in HUVEC were measured by real-time PCR. And the content of nitrogen monoxide (NO) of the supernatant was detected by nitrate reductasemethod accordingly.@*Results@#Twenty-four hours after infection, the mRNA expression of VCAM-1 in HUVECs of HCMV infected group increased obviously compared to control, and NO quantity increased accordingly and time-dependently. There was significant difference between groups(P<0.01).@*Conclusions@#HCMV increases the mRNA expression of VCAM-1 and quantity of NO, which may contribute to the formation of AS.

Effect of HSV-1 infection on the viability of the primary cultured chicken embryo telencephalon neurons / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To observe the effect of HSV-1 infection on the viability of the cultured chicken embryo telencephalon neurons in vitro.</p><p><b>METHODS</b>Primary culture model of chicken embryo of telencephalon neurons was established. Herpes simplex virus type 1 (HSV-1) was propagated in Vero cells and viral titer was measured by plaque forming method. Inverted microscope, transmission electron microscope, MTT cell viability and DNA agarose gels electrophoresis were used to evaluate the effect of HSV-1 infection on the neurons.</p><p><b>RESULTS</b>At 24, 48, 72 hours post infection the viability of the neurons decreased by 25%, 56%, 97% as compared with the control group. Morphological changes and DNA agarose gel electrophoresis showed a necrotic effect.</p><p><b>CONCLUSIONS</b>HSV-1 infection induced a remarkable decrease on the viability of neurons by necrosis rather than apoptosis.</p>

Effect of caspase-3 on the primary cultured mice cortical neurons with herpes simplex virus type-1 infection / 中华神经科杂志

Objective To study the effect of caspase-3 on the primary cultured mice cortical neuron w ith herpes simplex virus type 1 (HSV-1) infection. Methods A spectrophotometer was used to de tect the absorbance units at 405 nm of the caspase-3 substrate,the chrom o phore p-nitroanilide(pNA ).Comparing with the normal group, virus group, s orbital group,and sorbital group with virus in the absorbance units of chromophore substrate.The changes in inner mitochondrial membrane p otential of the different group cells were analyzed with flow cytometry. Results The released chromphore was measured by determining the absorbance at 405 nm. N group is 0.394 2, V group is 0.240 8, NS group is 0.539 2, VS gr oup is 0.465 0,the control C1 group is 0.290 0, C2 group is 0.235 0 and all groups were analyzed by statistic (P