Botulinum toxin promotes orofacial antinociception by modulating TRPV1 and NMDA receptors in adult zebrafish.

The aim of the study was to evaluate the possible involvement of transient receptor potential (TRP) channels, Acid-sensing ion channels (ASIC) and N-Methyl-D-aspartate receptor (NMDAR) in the orofacial antinociceptive behaviour effect of botulinum toxin type A (BoNT/A) in adult zebrafish. Initially, the open field test was performed to evaluate the effect of BoNT/A on the locomotor activity of zebrafish. Subsequently, the animals were pretreated with BoNT/A (0.05U, 0.1U or 0.5U/masseter) and acute orofacial nociception was induced by cinnamaldehyde, capsaicin, menthol, acid saline or glutamate applied to the lip or masseter muscle. In another group of experiments, animals were pre-treated with capsazepine (TRPV1 antagonist) or ketamine (NMDAR antagonist) to investigate the mechanism of antinociception. The possible involvement of central C-fibre afferents was also investigated using capsaicin desensitized animals. A molecular docking study was performed to observe the in silico interaction of BoNT/A with TRPV1 and NMDA channels. Pretreatment with BoNT/A reduced the nociceptive behaviour induced by capsaicin and glutamate. Antinociception was effectively inhibited by capsazepine and ketamine, as well as by capsaicin-induced desensitization. Consistent with these in vivo findings, the molecular docking study indicated that BoNT/A can interact with TRPV1 and NMDAR. The results indicate the involvement of TRP and NMDAR mechanisms in the orofacial antinociceptive behaviour effect of BoNT/A. The results also confirm the pharmacological relevance of BoNT/A as an inhibitor of orofacial nociception behaviour.

Effect of neutralization and hydrofluoric acid precipitate remotion on the compressive strength of monolithic lithium disilicate crowns.

BACKGROUND: The cleaning protocol for the ceramic surface after acid etching resulted in a decrease in bond strength and flexural strength of a glass ceramic. This study aims to evaluate the effect of different ceramic surface treatments after hydrofluoric acid etching (HF) on the compressive strength of monolithic lithium disilicate crowns. METHODS: Forty (40) human third molars received conventional full coverage preparation. After performing digital impressions of teeth preparations, ceramic blocks were machined using a CAD/CAM system in order to obtain the crowns. The crowns were distributed in 4 groups as ceramic surface treatment (N.=10): (HF) - 4.9% HF for 20s + air-water spray for 30s; (HFN) - HF + neutralizing agent for 5 min (N); (HFU) - HF + ultrasonic bath for 5 min (U); e (HFNU) - HF + N + U. SEM and EDS analysis was performed in each group in order to characterize the ceramic surface and to verify the chemical element distribution after HF cleaning protocols. A silane layer was applied (for 60s), and crowns were then cemented with dual resin cement. A compressive load was applied on the middle of the occlusal crown surface with a crosshead speed of 1 mm/min until fracture. Data were analyzed using ANOVA and Tukey test (α=0.05). RESULTS: Fluoride ions were found in samples of all postetching cleaning protocols. The mean value (Kgf) was: HF =169.92±21.37; HFN =187.34±34.79; HFU =166.63±40.22 and HFNU=175.26±40.22. The ceramic surface treatment after HF etching did not significantly influence (P>0.05) the compressive strength of the tested ceramic crowns. CONCLUSIONS: Surface treatments with neutralizing agent associated with the ultrasonic bath as the pre-cementation protocol was the most efficient protocol in eliminating the precipitate deposited on the porosities created by acid etching.

Evaluación de la Resistencia de Unión de Brackets Ortodónticos Fijados a Cerámica de Disilicato de Litio / Evaluation of Union Resistance of Orthodontic Bracelets Fixed to Lithium Dissylate Ceramic

RESUMEN: El objetivo de este trabajo consistió en evaluar la resistencia al cizallamiento de brackets ortodónticos cementados a una cerámica odontológica a base de disilicato de litio. Fueron confeccionadas 80 muestras rectangulares de cerámica vítrea a base de disilicato de litio. Las muestras fueron divididas inicialmente en 4 grupos de 20 discos según el tratamiento de superficie: ácido fosfórico 37 % por 30 segundos (HP); ácido hidrofluorhidro 10 % por 60 segundos (HF); óxido de aluminio durante 4 segundos (SB); y aspersión con punta diamantada durante 10 segundos (DW). Cada grupo fue dividido en dos subgrupos para la cementación de brackets Edgwise: metálico y cerámico. Los dos nuevos grupos fueron subdivididos en termociclados o no termociclados. Las muestras termocicladas pasaron por 500 ciclos con variaciones de temperatura entre 5 °C a 55 °C. Los valores numéricos obtenidos en la prueba de cizallamiento se sometieron al análisis estadístico descriptivo (media y desviación estándar), habiéndose realizado la prueba de normalidad Shapiro-Wilk y las pruebas no paramétricas de Kruskal-Wallis y Mann-Whitney. Se verificó que no hubo diferencia estadísticamente significativa en relación al tipo de tratamiento de superficie y la realización o no de termociclado, observándose diferencia estadísticamente significativa sólo en el tipo de bracket, siendo la resistencia de unión en el bracket metálico mayor que en el de cerámica. Así, el tipo de tratamiento de superficie y el envejecimiento por termociclaje no fueron decisivos en la resistencia de unión de brackets ortodónticos a la cerámica de disilicato de litio.

ABSTRACT: To evaluate the shear strength of cemented orthodontic brackets to a dental ceramic based on lithium disilicate. Rectangular samples 80 based glassceramic lithium disilicate were prepared. The samples were initially divided into 4 groups of 20 discs according to surface treatment: 37 % phosphoric acid for 30 seconds (HP); 10 % hydrofluoric acid for 60 seconds (HF); blasting with aluminum oxide for 4 seconds (SB); and diamond-tipped for 10 seconds (DW). Each group was divided into two subgroups for the Edgwise bracket cementation: metallic and ceramic. The two new groups were subdivided into thermocyclers or nonthermocyclics. The thermocycled samples passed 500 cycles with temperature variations between 5 ° C to 55 ° C. The numerical values obtained in the shear test were submitted to descriptive statistical analysis (mean and standard deviation), and the Shapiro-Wilk normality test and nonparametric Kruskal-Wallis and Mann-Whitney tests were performed. It was found that there was no statistically significant difference in the type of surface treatment and conducting or not thermocycling, observing statistically significant difference only on the bracket type, and the bond strength at higher metal bracket than in ceramics. Thus, the type of surface treatment and thermocycling aging were not decisive in the bond strength of orthodontic brackets to the lithium disilicate ceramic.

Evaluation of the Accuracy of Conventional and Digital Methods of Obtaining Dental Impressions / Evaluación de la Exactitud de los Métodos Convencionales y Digitales de la Obtención de Impresiones Dentales

ABSTRACT: The aim of this study was to carry out a literature review on the accuracy of conventional and digital dental impression methods, as well as present the various three-dimensional intraoral scanning systems. A bibliographic search was carried out in PUBMED's main health database, in which works published between 2008 and 2018 were collected. Laboratory studies, case reports and systematic reviews were included, addressing topics that deal with conventional digital materials, impression and precision. Articles that did not evaluate impression materials, their behavior and techniques to obtain a good impression of oral structures were excluded. Through a review in the literature, obtained the following findings: the most critical stage in macking a dental prothesis is to take the dental impression. The conventional technique of impression consists of obtaining a negative copy of the intra-oral situation that will be poured into gypsum, obtaining a positive copy, on which the work will be carried out. Digital scanning systems were not superior to conventional moldings when comparing fidelity, accuracy and detail reproduction; in contrast, they were superior to conventional impression when considering clinical chair time, patient and operator preference, and patient comfort.

RESUMEN: El objetivo de este estudio consistió en realizar una revisión bibliográfica sobre la precisión de los métodos de moldeo dental convencionales y digitales, así como presentar los diversos sistemas de escaneo intraoral tridimensionales. Se realizó una búsqueda bibliográfica en la base de datos de salud principal de PUBMED en la cual se recopilaron trabajos publicados entre 2008 y 2018. Se incluyeron estudios de laboratorio, informes de casos y revisiones sistemáticas, abordando temas que tratan con materiales digitales convencionales, moldeo y precisión. Se excluyeron los artículos que no evaluaron los materiales de moldeo, su comportamiento y técnicas para obtener una buena impresión de las estructuras orales. A través de una revisión exhaustiva en la literatura, obtuvimos los siguientes hallazgos: el paso más crítico en la preparación de una pieza protésica es la impresión de la preparación. La técnica convencional de moldeo consiste en obtener una copia negativa de la situación intraoral que se verterá en el yeso, obteniendo una copia positiva, sobre la cual se realizará el trabajo. Resulta que los sistemas de escaneo digital no fueron superiores a las molduras convencionales al comparar la fidelidad, precisión y reproducción de detalles; en cambio, fueron superiores a las molduras convencionales al considerar el tiempo de trabajo clínico, la preferencia del paciente y del operador y la comodidad del paciente.

Simplificando o registro interoclusal de arcos parcialmente desdentados com extremidades livres / Simplifying the interoclusal record of partially edentulous arches with free ends

A ausência de uma relação intercuspídica causada por perdas dentárias em um ou mais segmentos de arco na região posterior poderá trazer consigo, ao longo do tempo, prejuízos estéticos e funcionais que dificultarão o tratamento reabilitador. Neste contexto, poderão estar presentes extrusões dentárias, diminuição da DVO, sobremordida acentuada e alteração da conformação natural dos planos oclusais. Torna-se então fundamental a montagem de modelos de estudo em articulador semiajustável (ASA) para o planejamento do caso. Para que isto aconteça é necessário um registro interoclusal preciso que forneça suporte e estabilidade aos modelos de estudo na simulação da realidade clínica. O presente artigo relata breve descrição técnica de registro interoclusal com moldagem inicial simultânea para montagem de modelos de estudo em articulador numa única sessão clínica (AU).

The absence of an intercuspid relationship caused by dental losses in one or more arch segments in the posterior region may bring with it, over time, aesthetic and functional damages that will make rehabilitation treatment difficult. In this context, may be present dental extrusions, reduction of OVD, marked vertical overlap, and alteration of natural conformation of the occlusal planes. It is then fundamental to assemble semi-adjustable articulator study models (ASA) for case planning. For this to happen, a precise interocclusal record is needed to provide support and stability to the study models in the simulation of clinical reality. The present article reports a brief technical description of interocclusal record with simultaneous initial molding for assembly of study models in articulator in a single clinical session (AU).

A tecnologia 3D e suas aplicações na Odontologia moderna ­ revisão sistemática / 3D technology and its applications in modern Dentistry ­ systematic review

Identificar e apresentar diferentes tecnologias 3D utilizadas atualmente na Odontologia, avaliando as vantagens e limitações dessa ferramenta. A abordagem metodológica empregada consistiu de pesquisa sistemática realizada em diferentes motores de busca, como Pubmed e portal CAPES, e nas bases de dados Science Direct e Medline para coletar estudos clínicos que abordassem aplicações da manufatura aditiva em diferentes áreas da Odontologia. As palavras-chave para a busca textual foram Odontologia (Dentistry); Reabilitação Bucal (Oral Rehabilitation) e Tecnologia Odontológica (Dental Technology). A modalidade de pesquisa desse projeto foi conduzida por diretrizes contidas nas orientações para realização de revisões sistemáticas (PRISMA). Foram selecionados 11 artigos trazendo o uso da impressão 3D nas áreas de cirurgia e traumatologia bucomaxilofacial (n=5); prótese dentária (n=3) e Implantodontia (n=3). Como resultados, podemos dizer que os estudos demonstram a evolução das técnicas de manufatura aditiva, acompanhadas pelas suas aplicações, iniciando com a estereolitografia com a finalidade de planejamento reverso, treinamento da execução de determinado procedimento cirúrgico, até a sinterização seletiva a laser com a fabricação de objetos resistentes, como implantes osseointegrados, a serem aplicados como produto final no tratamento do paciente. A tecnologia aditiva ainda é incipiente, mas a tendência é ser cada vez mais utilizada e seus benefícios melhores aproveitados pela Odontologia (AU).

Identify and present different 3D technologies currently used in Dentistry, evaluating the advantages and limitations of this tool. The methodological approach employed consisted of systematic research conducted in different search engines, such as Pubmed and CAPES portal, and in the databases Science Direct and Medline to collect clinical studies that addressed additive manufacturing applications in different areas of Dentistry. The keywords for the textual search were Dentistry; Oral Rehabilitation; and Dental Technology. The research modality of this project was conducted by directives contained in the guidelines for conducting systematic reviews (PRISMA). Eleven articles were selected, demonstrating the use of 3D printing in the areas like buccomaxillofacial surgery and traumatology (n = 5); prosthodontics (n = 3); and Implantology (n = 3). As results, we can say that studies demonstrate the evolution of additive manufacturing techniques, followed by their applications, starting with stereolithography for the purpose of reverse planning, certain surgical procedure execution training until the selective laser sintering with the manufacturing of resistant objects, such as osseointegrated implants, to be applied as final product in the treatment of the patient. The additive technology is still incipient, but the tendency is to be increasingly used and that Dentistry takes advantage of its better benefits (AU).

Influence of post-etching surface treatment and thermo-mechanical cycling on fracture strength of ceramics.

BACKGROUNG: The aim of this study was to evaluate fracture strength of lithium disilicate-based ceramic crowns submitted to neutralization of hydrofluoric acid (HF) precipitates associated with ultrasonic bath and to thermo-mechanical cycling. METHODS: Eighty human molars received conventional full crown preparation after being included in polyurethane standard to simulate periodontal ligament. After scanning, the ceramic blocks were machined using CAD/CAM system to obtain the crowns. The crowns were distributed according to surface treatment: S and S-C; HF and silane; SNU and SNU-C; HF neutralization of HF precipitates, ultrasonic bath and silane. The crowns were cemented with dual cure resin cement and the specimens in Groups S-C and SNU-C were submitted to thermo-mechanical cycling (5/55 °C/60 s while 1.2 million mechanical cycles of 4 Hz/100 N). The samples were submitted to compressive test. Analysis of fractures was performed macroscopically (Burke method) and using Scanning Electron Microscope. The data of fracture strength were analyzed using the two-way ANOVA. RESULTS: No statistical difference among the groups was found (p-value = 0,799). Most failures were associated with the remaining tooth fracture and the fracture origin was located on the cementation surface. CONCLUSIONS: Postetching cleaning protocols do not improve the fracture strength of ceramics crowns. Thermo-mechanical aging did not weakened the ceramic crown.

Association between the facial type and morphology of the upper central incisor in normal occlusion subjects.

AIM: The purpose of this study was to verify the association between the facial type and the morphology of the upper central incisor, to potentially provide a guide for artificial tooth selection in esthetic and prosthetic rehabilitation. MATERIALS AND METHODS: The sample consisted of 51 lateral teleradiographs (lateral cephalometric radiographs) and cast models of subjects with a naturally optimal occlusion and at least four of the six keys of Andrews, for optimal occlusion. The facial type was defined by two cephalometric measurements (SN.Gn and SN.GoGn) and classified into dolichofacial, meso- facial and brachyfacial after digitizing the radiographs. The incisor morphology was classified into quadrangular, oval and triangular after 3D digitizing the models and evaluation of the images by 12 dentists. The evaluation between the examiners was performed by Kappa test. In order to test the differences between the variables, it was used a chi-square test (5%). RESULTS: No significant difference (p < 0.05) was found between the studied variables. CONCLUSION: Facial type was not associated with the morphology of the upper central incisor, considering the methodology and population included in the present study. CLINICAL SIGNIFICANCE: Using the facial type as a way to select the morphology of the upper central incisor proved to be inadequate in this study.

Cementation of ceramics and indirect composite resin to enamel and dentin using different resin-based cements ? Shear bond strength / Cimentação de cerâmica e resina composta indireta ao esmalte à dentina usando diferentes cimentos resinosos - resistência ao cisalhamento

Objective: To evaluate the shear bond strength of three different resin cements: self-etching (P), etch-and-rinse (R) and self-adhesive (U), to enamel and dentin using two types of restorative materials. Methods: One hundred twenty bovine incisors had the buccal surface ground to obtain a flat and smooth surface of approximately 25 mm2. Half of the specimens had the enamel exposed (E), and the other half had the dentin exposed (D). An ad-hesive tape with a 3 mm-diameter orifice delimited the bonding area. Sixty 3 x 3 mm lithium disilicate glass-ceramic cylinders (C) and 60 indirect composite resin cylinders (R) were cemented to enamel or dentin using three types of resin cements, resulting in 12 experimental groups represented by symbols allusive to treatment protocols: PEC; PER; PDC; PDR; REC; RER; RDC; RDR; UEC; UER; UDC; and UDR. Shear bond strength test was performed with a crosshead speed of 0.5 mm/min. The data were subjected to ANOVA and Tukey?s test with a significance level of 5%. Results (MPa ± DP): the highest mean value obtained, REC group (17.46 ± 3.29), differed significantly from others, except for the PEC group (14.60 ± 5.88). Among the groups with lower mean values, there was a predominance of those where the indirect resin was used regardless the substrate and type of resin cement, and the UDR group (1.03 ± 1.03) was statistically different from the others. Conclusion: In this study, all three experimental variables tested influenced the shear bond strength of tooth-cement-restoration interfaces.

Effect of cement shade and light-curing unit on bond strength of a ceramic cemented to dentin.

PURPOSE: To evaluate the effect of cement shade, light-curing unit, and water storage on tensile bond strength (σ) of a feldspathic ceramic resin bonded to dentin. MATERIALS AND METHODS: The dentin surface of 40 molars was exposed and etched with 37% phosphoric acid, then an adhesive system was applied. Forty blocks of feldspathic ceramic (Vita VM7) were produced. The ceramic surface was etched with 10% hydrofluoric acid for 60 s, followed by the application of a silane agent and a dual-curing resin cement (Variolink II). Ceramic blocks were cemented to the treated dentin using either A3 or transparent (Tr) shade cement that was activated using either halogen or LED light for 40 s. All blocks were stored in 37°C distilled water for 24 h before cutting to obtain non-trimmed bar-shaped specimens (adhesive area = 1 mm2 ± 0.1) for the microtensile bond strength test. The specimens were randomly grouped according to the storage time: no storage or stored for 150 days in 37°C distilled water. Eight experimental groups were obtained (n = 30). The specimens were submitted to the tensile bond strength test using a universal testing machine at a crosshead speed of 1 mm/min. The data were statistically analyzed using ANOVA and Tukey's posthoc tests (a = 0.05). RESULTS: The mean bond strength values were significantly lower for the corresponding water stored groups, except for the specimens using A3 resin cement activated by halogen light. There was no significance difference in mean bond strength values among all groups after water storage. CONCLUSION: Water storage had a detrimental effect under most experimental conditions. For both cement shades investigated (Tr and A3) under the same storage condition, the light-curing units (QTH and LED) did not affect the mean microtensile bond strengths of resin-cemented ceramic to dentin.

Fracture resistance of bovine incisors restored with different glass fiber posts: effect of the diameter of fiber post.

AIM: Compare the effect of three post designs on the fracture resistance and failure modes of composite core - fiber post - crownless tooth sets. MATERIALS AND METHODS: Ninety bovine incisors were selected and divided into nine groups of 10 specimens. The teeth were assigned to three groups based on the post design: Cylindrical, tapered, and double-tapered. Each group was subdivided into three subgroups in accordance with the diameter of the post: Small (No.1), medium (No.2), and large (No.3). The Panavia F system was used for post cementation. The specimens were mounted in acrylic resin blocks with a layer of silicone rubber covering the roots. A universal testing machine compressively loaded the specimens from the palatal side at a crosshead speed of 1 mm/min and at an angle of 135Ί to the long axis of the teeth, until failure occurred. The failure mode was determined by a stereomicroscope inspection of all the specimens. Data were analyzed by one-way ANOVA and the Tukey test (P < 0.05). RESULTS: The fracture resistance was affected by the type of post (P < 0.0001). A narrower diameter for all of the post systems allowed for higher resistance. The main failure mode in the large cylindrical group was catastrophic fractures, while the main failures in the other eight groups were favorable. CONCLUSION: Narrower diameter posts showed higher fracture resistance. The dominant failure pattern was repairable fracture, except for those with large cylindrical groups.

Resistance to compression of weakened roots subjected to different root reconstruction protocols

OBJECTIVE: This study evaluated, in vitro, the fracture resistance of human non-vital teeth restored with different reconstruction protocols. MATERIAL AND METHODS: Forty human anterior roots of similar shape and dimensions were assigned to four groups (n=10), according to the root reconstruction protocol: Group I (control): non-weakened roots with glass fiber post; Group II: roots with composite resin by incremental technique and glass fiber post; Group III: roots with accessory glass fiber posts and glass fiber post; and Group IV: roots with anatomic glass fiber post technique. Following post cementation and core reconstruction, the roots were embedded in chemically activated acrylic resin and submitted to fracture resistance testing, with a compressive load at an angle of 45º in relation to the long axis of the root at a speed of 0.5 mm/min until fracture. All data were statistically analyzed with bilateral Dunnett's test (α=0.05). RESULTS: Group I presented higher mean values of fracture resistance when compared with the three experimental groups, which, in turn, presented similar resistance to fracture among each other. None of the techniques of root reconstruction with intraradicular posts improved root strength, and the incremental technique was suggested as being the most recommendable, since the type of fracture that occurred allowed the remaining dental structure to be repaired. CONCLUSION: The results of this in vitro study suggest that the healthy remaining radicular dentin is more important to increase fracture resistance than the root reconstruction protocol.

Effect of carbon and glass fiber posts on the flexural strength and modulus of elasticity of a composite resin.

The aim of this study was to evaluate the effect of prefabricated fiber posts on the flexural strength and modulus of elasticity of a composite resin. Thirty bar-shaped specimens measuring 25 x 2.0 x 2.0 mm were made, containing posts that were 1.3 mm in diameter and 20 mm long. Each group contained 10 specimens: Group 1, resin without post; Group 2, resin with carbon fiber post; Group 3, resin with glass fiber post. The samples were immersed in water at 37 degrees C until the three-point loading test was performed at a speed of 1.0 mm/minute. The results were statistically analyzed by ANOVA and Tukey's test (P = 0.05). Both fiber posts were similar in strength and both were stronger than the control. Group 3 obtained a higher mean modulus of elasticity than Groups 1 and 2, which were similar. The results of this study demonstrated that the presence of a fiber post significantly raised flexural strength values and the glass fiber post significantly increased the modulus of elasticity of the evaluated composite resin.

Effect of hydrofluoric acid etching duration on the roughness and flexural strength of a lithium disilicate-based glass ceramic.

The aim of this study was to examine the effect of different acid etching times on the surface roughness and flexural strength of a lithium disilicate-based glass ceramic. Ceramic bar-shaped specimens (16 mm x 2 mm x 2 mm) were produced from ceramic blocks. All specimens were polished and sonically cleaned in distilled water. Specimens were randomly divided into 5 groups (n=15). Group A (control) no treatment. Groups B-E were etched with 4.9% hydrofluoric acid (HF) for 4 different etching periods: 20 s, 60 s, 90 s and 180 s, respectively. Etched surfaces were observed under scanning electron microscopy. Surface profilometry was used to examine the roughness of the etched ceramic surfaces, and the specimens were loaded to failure using a 3-point bending test to determine the flexural strength. Data were analyzed using one-way ANOVA and Tukey's test (?=0.05). All etching periods produced significantly rougher surfaces than the control group (p<0.05). Roughness values increased with the increase of the etching time. The mean flexural strength values were (MPa): A=417 ± 55; B=367 ± 68; C=363 ± 84; D=329 ± 70; and E=314 ± 62. HF etching significantly reduced the mean flexural strength as the etching time increased (p=0.003). In conclusion, the findings of this study showed that the increase of HF etching time affected the surface roughness and the flexural strength of a lithium disilicate-based glass ceramic, confirming the study hypothesis.

Resistance to compression of weakened roots subjected to different root reconstruction protocols.

OBJECTIVE: This study evaluated, in vitro, the fracture resistance of human non-vital teeth restored with different reconstruction protocols. MATERIAL AND METHODS: Forty human anterior roots of similar shape and dimensions were assigned to four groups (n=10), according to the root reconstruction protocol: Group I (control): non-weakened roots with glass fiber post; Group II: roots with composite resin by incremental technique and glass fiber post; Group III: roots with accessory glass fiber posts and glass fiber post; and Group IV: roots with anatomic glass fiber post technique. Following post cementation and core reconstruction, the roots were embedded in chemically activated acrylic resin and submitted to fracture resistance testing, with a compressive load at an angle of 45º in relation to the long axis of the root at a speed of 0.5 mm/min until fracture. All data were statistically analyzed with bilateral Dunnett's test (α=0.05). RESULTS: Group I presented higher mean values of fracture resistance when compared with the three experimental groups, which, in turn, presented similar resistance to fracture among each other. None of the techniques of root reconstruction with intraradicular posts improved root strength, and the incremental technique was suggested as being the most recommendable, since the type of fracture that occurred allowed the remaining dental structure to be repaired. CONCLUSION: The results of this in vitro study suggest that the healthy remaining radicular dentin is more important to increase fracture resistance than the root reconstruction protocol.

Effect of hydrofluoric acid etching duration on the roughness and flexural strength of a lithium disilicate-based glass ceramic

The aim of this study was to examine the effect of different acid etching times on the surface roughness and flexural strength of a lithium disilicate-based glass ceramic. Ceramic bar-shaped specimens (16 mm x 2 mm x 2 mm) were produced from ceramic blocks. All specimens were polished and sonically cleaned in distilled water. Specimens were randomly divided into 5 groups (n=15). Group A (control) no treatment. Groups B-E were etched with 4.9 percent hydrofluoric acid (HF) for 4 different etching periods: 20 s, 60 s, 90 s and 180 s, respectively. Etched surfaces were observed under scanning electron microscopy. Surface profilometry was used to examine the roughness of the etched ceramic surfaces, and the specimens were loaded to failure using a 3-point bending test to determine the flexural strength. Data were analyzed using one-way ANOVA and Tukey's test (?=0.05). All etching periods produced significantly rougher surfaces than the control group (p<0.05). Roughness values increased with the increase of the etching time. The mean flexural strength values were (MPa): A=417 ± 55; B=367 ± 68; C=363 ± 84; D=329 ± 70; and E=314 ± 62. HF etching significantly reduced the mean flexural strength as the etching time increased (p=0.003). In conclusion, the findings of this study showed that the increase of HF etching time affected the surface roughness and the flexural strength of a lithium disilicate-based glass ceramic, confirming the study hypothesis.

O objetivo deste estudo foi avaliar o efeito de diferentes tempos de condicionamento ácido na rugosidade de superfície e resistência flexural de uma cerâmica à base de disilicato de lítio. Espécimes cerâmicos em forma de barra (16 mm x 2 mm x 2 mm) foram produzidos a partir de blocos cerâmicos. Todos os espécimes foram polidos e limpos em banho de ultrasom em água destilada. Os espécimes foram aleatoriamente divididos em 5 grupos (n=15). Grupo A (controle) sem tratamento. Grupos B-E condicionamento com ácido fluorídrico 4,9 por cento (HF) por 4 diferentes períodos de condicionamento: 20 s, 60 s, 90 s e 180 s, respectivamente. As superfícies condicionadas foram observadas sob microscopia eletrônica de varredura. Perfilometria de superfície foi utilizada para examinar a rugosidade das superfícies condicionadas, e os espécimes foram carregados até a falha pelo teste de flexão três pontos. Os valores foram analisados usando ANOVA um fator e teste de Tukey (?=0,05). Todos os períodos de condicionamento produziram superfícies significantemente mais rugosas do que o grupo controle (p<0,05). Os valores de rugosidade aumentaram com o tempo de condicionamento. Os valores médios de resistência à flexão foram (MPa): A=417 ± 55; B=367 ± 68; C=363 ± 84; D=329 ± 70; e E=314 ± 62. O condicionamento com HF reduziu significativamente os valores médios de resistência à flexão conforme o tempo de condicionamento aumentou (p=0,003). Os achados deste estudo mostraram que o aumento do tempo de condicionamento ácido influenciou a rugosidade de superfície e resistência flexural de uma cerâmica à base de disilicato de lítio confirmando a hipótese do mesmo.

Bond strength of a composite resin to an adhesive luting cement.

This study evaluated the influence of surface treatment on the shear bond strength of a composite resin (CR), previously submitted to the application of a temporary cement (TC), to an adhesive luting cement. Eight-four CR cylinders (5 mm diameter and 3 mm high) were fabricated and embedded in acrylic resin. The sets were divided into 6 groups (G1 to G6) (n=12). Groups 2 to 6 received a coat of TC. After 24 h, TC was removed and the CR surfaces received the following treatments: G2: ethanol; G3: rotary brush and pumice; G4: air-abrasion; G5: air-abrasion and adhesive system; G6: air-abrasion, acid etching and adhesive system. G1 (control) did not receive TC or any surface treatment. The sets were adapted to a matrix and received an increment of an adhesive luting cement. The specimens were subjected to the shear bond strength test. ANOVA and Tukey's tests showed that G3 (8.53 MPa) and G4 (8.63 MPa) differed significantly (p=0.001) from G1 (13.34 MPa). The highest mean shear bond strength values were found in G5 (14.78 MPa) and G6 (15.86 MPa). Air-abrasion of CR surface associated with an adhesive system provided an effective bond of the CR to the adhesive luting cement, regardless the pre-treatment with the phosphoric acid.

Efeito da neutralização e remoção sônica do precipitado do ácido fluorídrico na resistência à fratura de coroas cerâmicas / Effect of neutralization and sonic remotion of the hydrofluoric acid precipitate on the fracture strength of ceramic crowns

Testou-se o efeito de diferentes formas de tratamento da superfície cerâmica após condicionamento com ácido hidrofluorídrico (HF) na resistência à fratura de coroas cerâmicas à base de dissilicato de lítio. Quarenta terceiros molares humanos receberam um preparo convencional para coroa total. Após escaneamento digital dos preparos, blocos cerâmicos foram usinados pelo sistema CAD/CAM para obtenção das coroas. A espessura da película de cimento das coroas ao preparo foi verificada com silicone de adição. As coroas foram distribuídas nos 4 grupos conforme o tratamento da superfície cerâmica (n=10): (HF)- HF à 4.9% por 20s + spray ar-água por 30s; (HFN)– HF + agente neutralizador por 5 min (N); (HFU) – HF + banho sônico por 5 min (U); e (HFNU)– HF +N + U. Após aplicação de uma camada de silano (60s), as coroas foram cimentadas com cimento resinoso dual. Uma carga compressiva de 1000 Kg foi aplicada no centro da face oclusal da coroa com velocidade de 1 mm/min até a fratura. Os dados foram analisados por ANOVA e Tukey (α=0,05). A carga média de fratura (Kgf) foi: HF = 169,92 ± 21,37; HFN =187,34 ± 34,79; HFU = 166,63 ± 40,22 e HFNU = 175,26 ± 40,22. O tratamento da superfície cerâmica após condicionamento ácido não influenciou significativamente (p>0.05) na resistência à fratura das coroas cerâmicas testadas, sugerindo que é desnecessário qualquer tratamento adicional para remoção de resíduos após o uso de HF e lavagem com jato de água-ar.

The effect of different ceramic surface post hydrofluoric acid (HF) etching cleaning protocols in the fracture strength of lithium dissilicate glass ceramic crowns were evaluated. Forty maxillary third human molars received aconventional full preparation. Crowns were obtained from the digital scanning ofthe preparations and CAD/CAM blocks machining. Crown cement film thickness was evaluated with vinyl polysiloxane. The crowns were allocated in 4 groups according to the ceramic surface treatment (n=10): (HF)- 4.9% HF for 20s +water spray for 30s; (HFN)- HF + neutralizing agent for 5 min (N); (HFU) -: HF+sonic bath for 5 min (U) and (HFNU): HF + N + U. After a silane coat (60s), crowns were cemented with dual cure cement. A compressive load of 1000 Kg was applied in the occlusal surface center at crosshead speed of 1mm/min untilthe fracture. Data were analyzed by one-way ANOVA and Tukey tests (α = 0.05). Fracture strength mean for each group was (Kgf): HF = 169.92 ± 21.37; HFN =187.34 ± 34.79; HFU = 166.63 ± 40.22 e HFNU = 175.26 ± 40.22. There was no statistical difference as the surface treatment (p>0.05) suggesting that it is not necessary any additional ceramic surface treatment in order to remove debris after using of HF and air-water spray rinsing.

Bond strength of a composite resin to an adhesive luting cement

This study evaluated the influence of surface treatment on the shear bond strength of a composite resin (CR), previously submitted to the application of a temporary cement (TC), to an adhesive luting cement. Eight-four CR cylinders (5 mm diameter and 3 mm high) were fabricated and embedded in acrylic resin. The sets were divided into 6 groups (G1 to G6) (n=12). Groups 2 to 6 received a coat of TC. After 24 h, TC was removed and the CR surfaces received the following treatments: G2: ethanol; G3: rotary brush and pumice; G4: air-abrasion; G5: air-abrasion and adhesive system; G6: air-abrasion, acid etching and adhesive system. G1 (control) did not receive TC or any surface treatment. The sets were adapted to a matrix and received an increment of an adhesive luting cement. The specimens were subjected to the shear bond strength test. ANOVA and Tukeyʼs tests showed that G3 (8.53 MPa) and G4 (8.63 MPa) differed significantly (p=0.001) from G1 (13.34 MPa). The highest mean shear bond strength values were found in G5 (14.78 MPa) and G6 (15.86 MPa). Air-abrasion of CR surface associated with an adhesive system provided an effective bond of the CR to the adhesive luting cement, regardless the pre-treatment with the phosphoric acid.

A influência do tratamento de superfície sobre a resistência adesiva entre uma resina composta (RC), previamente submetida à aplicação de um cimento temporário (CT), e um cimento resinoso foi avaliada. Oitenta e quatro cilindros de RC (5 mm de diâmetro e 3 mm de altura) foram confeccionados e incluídos em resina acrílica. Os conjuntos foram divididos em 6 grupos (G1 a G6) (n=12). Os grupos de 2 a 6 receberam uma camada do CT. Após 24 h, o CT foi removido e as superfícies de RC receberam os seguintes tratamentos: G2: limpeza com etanol; G3: limpeza com escova rotatória e pedra pomes; G4: jateamento ; G5: jateamento e aplicação de adesivo; G6: jateamento, condicionamento ácido e adesivo. O G1(controle) não recebeu CT ou nenhum tratamento de superfície. Os conjuntos foram adaptados à matriz e receberam incremento de cimento resinoso. Os corpos de prova foram submetidos ao ensaio de cisalhamento. Os testes ANOVA e Tukey (p=0,001), demonstraram que os grupos G3 (8,53 MPa) e G4 (8,63 MPa) diferiram estatisticamente do grupo G1 (13,34 MPa). As maiores valores médios de resistência ao cisalhamento foram encontradas nos grupos G5 (14,78 MPa) e G6 (15,86 MPa). O jateamento da superfície da resina composta associado a um sistema adesivo, independente do pré-tratamento com o ácido fosfórico, proporcionou uma efetiva união ao cimento resinoso.