Identification and Characterization of a New Microalga Dysmorphococcus globosus-HI from the Himalayan Region as a Potential Source of Natural Astaxanthin.

Synthesized astaxanthin (ASX), stereoisomers of 3S,3'R, 3R,3'R, and 3S,3'S, have over 95% market share and have relatively poor antioxidant and bioactivity properties, with persistent issues in terms of biological functions, health benefits, and biosafety if compared to natural ASX. Bioprospecting of new microalgal strains could be vital for a new source of powerful antioxidant (ASX). In this study, a new algal strain was isolated from the Indian foothills of the Himalayas. Its identity was discerned by morphological and DNA barcode studies. It is a unicellular spheroidal cell-shaped alga with 100-200 µm diameter. The isolate has 93.4% similarity to Dysmorphococcus globosus species based on 18S-rDNA phylogenetic analysis and named as D. globosus-HI (HI stands for Himalayan India). Its growth and major cellular components (carotenoids, carbohydrates, protein, lipids, fatty acid profile, and ASX) were optimized using the seven different culture media. The highest biomass (1.14 g L-1) was observed in the MBBM medium, with a specific growth rate (0.087 day-1), division/day (0.125), and cellular yield (6.16 x 106 cells/mL). The highest carotenoids (1.56 mg g-1), lipids (32.5 mg L-1), and carbohydrates (135.62 mg L-1) were recorded in the 3N-BBM medium. The maximum ω3-FAs (17.78%), ω6-FAs (23.11%), and ω9-FAs (7.06%) were observed in MBBM, JW, and BG-11 medium respectively. The highest amount of antioxidant ASX was accumulated in the 3N-BBM medium (391 mg L-1). It is more than any other known algal species used in the production of natural ASX. The optimized biochemical studies on the D. globosus-HI strain should fulfill the increasing demand for natural ASX for commercial application.

Critical evaluation of functional aspects of evaporation barriers through environmental and economics lens for evaporation suppression - A review on milestones from improved technologies.

Climate change models predict an increase in rainfall variability, leading to floods and drought events, hence intensifying the need for reservoirs. However, up to 50% of reservoirs' capacity is lost by evaporation, affecting their function of ensuring water availability and stability. Over decades biological, chemical and physical barriers "covers" were developed for inhibiting evaporation. Such barrier's efficiency and applicability are still a matter of discussion, given their economic efficiency, environmental consequences, and operational difficulties are accounted for. In this review, we discussed the efficiency, applicability, and environmental suitability of these covers. Compared to the physical covers, the chemical and biological solutions tend to be less efficient. However, the use of physical covers is multidisciplinary, involving climate, material, and hydrological sciences, and are more efficient. Among the physical covers, the use of suspended covers and free-floating elements decreases evaporation to the tune of 85 and 80.0%, respectively. However, the economic efficiency of free-floating elements remains an open question since all studies overlooked their water footprint (water used in the manufacturing process of these covers), which was found to be very high. The use of these covers decreases heat storage, gas exchange rate, and light availability that could adversely influence dissolved oxygen, water quality, aquatic organisms, and the water ecosystem's function. These ecological consequences have not yet been investigated. The exception is the suspended covers, which have had determinate effects on dissolved oxygen and algae growth. Due to light weight, floating elements' operation is unstable and vulnerable to move due to wind effects. Therefore, such covers must be engineered to increase their stability. Free-floating elements could provide a visible and scalable solution to evaporation suppression when considering their economic visibility, environmental effects, and stability against wind and wave effects under the field conditions. However, these covers can be viable only when water availability is the limiting factor in crop production. We found that studies at reservoir scale are highly limited, therefore, investigations at reservoirs' scale emphasizing ecological aspects, cover stability and cost efficiency, are urgently needed.