[Study on the diagnostic value of imageology of giant cell tumour of tendon sheath].

OBJECTIVE: To investigate the diagnostic value of imageology of giant cell tumour of tendon sheath (GCTS) including X-ray, CT and MRI. METHODS: Thirty-five patients with GCTTS confirmed by operation and pathology were retrospectively analyzed. There were 16 males and 19 females. The average age was 39.4 years, ranged from 7 to 66 years. All the patients underwent X-ray examination, 8 patients underwent CT examination, and 16 patients underwent MRI examination. RESULTS: There were 2 patients in knee joint, 6 patients in ankle joint, 1 patient in capitulum radius, 2 patients in wrist joint, 14 patients in hand and 10 patients in foot. Ten cases were the diffuse form, and 25 cases were the focal form. The X-ray results: the slightly high density soft tissue mass surrounding the bone were shown in 32 cases, 3 cases were normal. The bone erosion were shown in 9 cases, the obvious destruction of bone were shown in 5 cases. CT results: The soft tissue mass and the destruction of bone were shown clearly. MRI results: On T1WI, the signal intensity of GCTTS almost was similar to those of skeletal muscle in 9 cases and was slightly lower than those of skeletal muscle in 7 cases. On T2WI, the signal intensity presented mainly hypointensity with patchy isointensity or hyperintensity signal. A little of fluid was shown in 6 cases. CONCLUSION: X-ray can demonstrate the lesion and erosion of bone, destruction of bone can clearly be shown on CT. The low intensity signal on MRI T1WI and T2WI is the characteristic appearance of GCTTS. And it can clearly show the lesion range and type of GCTTS.

[Synthesizing A beta(1-15) multiple antigen peptide vaccine by indirect conjugation and its immunological activity].

AIM: To explore the method of synthesizing the A beta(1-15) multiple antigen peptide (MAP) vaccine and to identify its quality and the immunological activity. METHODS: MAP A beta(1-15) was synthesized by indirect conjugation and analyzed by RP-HPLC,SDA-PAGE and amino acid analysis. Then, C57BL/6 mice were immunized with synthesized MAP A beta(1-15). The specific anti-A beta antibody in the sera of the immunized mice was identified by ELSA. RESULTS: There was a high and wide peak wave in the RP-HPLC chromatogram. The 8 protein bands identified by SDA-PAGE was identical with 1 to 8 branch of MAP A beta(1-15). The amino acid sequence of synthesized MAP A beta(1-15) was almost similar with the standard. High titer of anti A beta antibody was obtained in the C57BL/6 mice immunized with MAP A beta(1-15). CONCLUSION: MAP A beta(1-15) could be synthesized successfully by indirect conjugation and the synthesized MAP A beta(1-15) had satisfactory immune activity. But the purification of the synthesized complex remained to be a problem.