[The surveillance of nosocomial bacteremia in the Intensive Care Unit of the Hospital Pediátrico Docente Centro Habana]. / Vigilancia de la bacteriemia nosocomial en la Unidad de Cuidados Intensivos del Hospital Pediátrico Docente Centro Habana.

A prospective study was carried out in order to assess nosocomial bacteremia in the Intensive Care Unit of the Centrohabana Teaching Pediatric Hospital, from January to May 1988. 66.7% of the bacteremia episodes diagnosed were of a nosocomial origin, mostly secondary. Nosocomial bacteremia rate was 15.5 per 100 admissions, with predominance in the age group under 1 year of age. Risk factors for acquiring nosocomial bacteremia were hospital stay longer than 72 hours, age under 1 year, tracheal intubation, deep venous catheterization and urinary catheterization. The most frequently associated microorganisms were Staphylococcus epidermis, Escherichia coli and Pseudomonas aeruginosa.

[Epidemiologic markers in the study of Pseudomonas aeruginosa from a pediatric intensive care unit]. / Marcadores epidemiológicos en el estudio de Pseudomonas aeruginosa procedentes de una unidad de cuidados intensivos pediátrica.

A study was made on 198 Pseudomonas aeruginosa strains isolated at a pediatric intensive care unit from January to August 1988, using pyocin typing and antibiotic typing as markers. The most frequent circulating pyocin types were 31.83 and atypical 23578. The germ showed high resistance in vitro to the antimicrobial drugs used and the resistance patterns were distributed in 19 different antibiotic types.

[Microbiologic aspects of bacteremia in patients hospitalized in the intensive care unit]. / Aspectos microbiologicos de la bacteriemia en pacientes ingresados en la Unidad de Cuidados Intensivos.

The microbiologic aspects of the bacteriemia were prospectively studied in patients hospitalized in a pediatric Intensive Care Unit. Thirty six episodes of bacteriemia were detected in 29 patients. Secondary bacteriemia prevailed on the primary ones. The most frequent infectious foci associated with bacteriemia were the infections of respiratory tract, followed by intravascular catheterism. The microorganisms more frequently associated with bacteriemia were: Haemophilus influenzae, Staphylococcus epidermidis, Eacherichia coli and Pseudomonas seruginosa.

[Identification of non-fermenting gram-negative bacteria. "Havana Center" Pediatric Teaching Hospital. December 1986 through May 1987]. / Identificación de bacterias gramnegativas no fermentadoras. Hospital Pediátrico Docente "Centro Habana". Diciembre de 1986 a mayo de 1987.

A study is made of non-fermenting gram-negative bacterial strains isolated at the Microbiology Lab, "Centro Habana" Pediatric Teaching Hospital. The strains studied come from various types of samples with prevalence of exudates and smears. A definite biotyping of 100% of strains is made. 90.0% are classified within the genus Pseudomonas. In addition, organisms of the genera Acinetobacter, Alcaligenes, Achromobacter, and Moraxella are identified. The most frequent species turned out to be Pseudomonas aeruginosa, which accounted for 68.4% of the total.

[Isolation of beta lactamase enzyme in strains of Staphylococcus aureus with the method of starch paper]. / Detección de la enzima beta lactamasa en cepas de Staphylococcus aureus por el método del papel de almidón.

The authors worked with 2,273 strains of Staphylococcus aureus isolated from clinical samples processed at the Microbiology laboratory of Centro Habana Pediatric Teaching Hospital in the period ranging from June 1985 to December 1986. All strains underwent the bacterial sensitivity test against antibiotics by Bauer-Kirby technique. Fourty-eight strains were sensitive to penicillin - which accounts for 2.11% - by the beta-lactamase detection test by the starch paper method. In all cases, negative results were obtained, which is in agreement with the results of the sensitivity tests.

[Introduction of King A and King B media for the diagnosis of Pseudomonas aeruginosa at the Teaching Pediatric Hospital of Centro Habana]. / Introducción de los medios King A y King B para el diagnóstico de Pseudomonas aeruginosa en el Hospital Pediátrico Docente de Centro Habana.

The use of King A and King B media for the faster diagnosis of Pseudomonas aeruginosa is introduced in the Microbiology Laboratory of Centro Habana Pediatric Teaching Hospital. The appropriate use of these media allows to classify as Pseudomonas aeruginosa 77.2% of the Pseudomonas sp. strains studied, which represent 95.2% of the total of strains identified as Pseudomonas aeruginosa, according to Hugh and Gilardi conventional technique. There are no significant differences in the implementation of the two procedures (X2: 0.5; p less than 0.05). The demonstrations of pyocines with King A medium is significantly superior to the method for extraction and solubility of the pigment in chloroform (X2: 15.05; p less than 0.01).

Encuesta serologica a ninos inmunizados con vacuna antipertussis en Ciudad de la Habana. / Serologic searching in children immunized with antipertussis vaccine at Havana City

Se realizo la busqueda de anticuerpos aglutinantes antipertussis en 480 sueros sanguineos, procedentes de ninos de 0 a 14 anos, inmunizados con la vacuna triple (DPT) en la Ciudad de La Habana.Se utilizo la tecnica de aglutinacion en tubo, con diluciones de suero al duplo, utilizando 10 tubos, se comenzo con la dilucion de 1:20. Se empleo el antigeno para la reaccion de aglutinacion de Bortedella pertussis preparado en el INHEM. De los 480 sueros estudiados, en el 71% se observo aglutinacion y en 174 (36,2%) con titulos de 1:320 o mas. Se relacionan los resultados del estudio serico con los grupos de edades