Reduced intestinal FADS1 gene expression and plasma omega-3 fatty acids following Roux-en-Y gastric bypass.

BACKGROUND & AIMS: Roux-en-Y gastric bypass (RYGB) limits food ingestion and may alter the intestinal expression of genes involved in the endogenous synthesis of polyunsaturated fatty acids (PUFAs). These changes may decrease the systemic availability of bioactive PUFAs after RYGB. To study the impact of RYGB on the dietary ingestion and plasma concentration of PUFAs and on the intestinal expression of genes involved in their endogenous biosynthesis in severely obese women with type 2 diabetes. METHODS: Before, and 3 and 12 months after RYGB, obese women (n = 20) self-reported a seven-day dietary record, answered a food frequency query and provided plasma samples for alpha-linolenic (ALA), eicosapentaenoic (EPA), docosahexaenoic (DHA) and arachidonic (ARA) acid assessment by gas chromatography. Intestinal biopsies (duodenum, jejunum and ileum) were collected through double-balloon endoscopy before and 3 months after RYGB for gene expression analysis by microarray (Human GeneChip 1.0 ST array) and RT-qPCR validation. RESULTS: Compared to the preoperative period, patients had decreased intakes of PUFAs, fish and soybean oil (p < 0.05) and lower plasma concentrations of ALA and EPA (p < 0.001) 3 and 12 months after RYGB. FADS1 gene expression was lower in duodenum (RT-qPCR fold change = -1.620, p < 0.05) and jejunum (RT-qPCR fold change = -1.549, p < 0.05) 3 months following RYGB, compared to before surgery. CONCLUSION: RYGB decreased PUFA ingestion, plasma ALA and EPA levels, and intestinal expression of FADS1 gene. The latter encodes a key enzyme involved in endogenous biosynthesis of PUFAs. These data suggest that supplementation of omega-3 PUFAs may be required for obese patients undergoing RYGB. Clinical Trial Registry number and website: www.clinicaltrials.gov - NCT01251016; Plataforma Brasil - 19339913.0.0000.0068.

Efficiency of the 24-hour food recall instrument for assessing nutrient intake before and after Roux-en-Y gastric bypass.

BACKGROUND: Nutritional and food intake assessments before and after bariatric surgery may be important to correct eating habits and nutritional deficiencies. AIM: To assess the efficiency of the 24-hour food recall (24hR) form as a fast method for assessing nutrient intake before and after a Roux-en-Y gastric bypass (RYGB). METHODS: This study analyzed data from 10 obese patients with type 2 diabetes mellitus (T2DM). Food intake (calories, macronutrients, and micronutrients) before and 3 months after RYGB were assessed with the 24hR as well as the seven-day food record (7dR) as the gold standard reference. Virtual Nutri Plus® software was used to quantify nutrients. RESULTS: The 7dR data revealed deficits in the estimated intake of total fiber and 14 out of 22 micronutrients pre- and postoperatively, combined; the 24hR failed to detect intake deficits in only two of these micronutrients (vitamins A and B3). Other postoperative deficits included carbohydrates, vitamin B1, copper, and iron, on which the 24hR was sensitive to only the iron deficit. In our pre- versus post-operative comparison analyses, the 7dR revealed decreases in total calories, carbohydrates, total and all subclasses of fat and fiber, and 12 micronutrients; in the analogous comparison analyses, the 24hR failed to detect decreases in the ingestion of monounsaturated and saturated fats and in six of these micronutrients. CONCLUSIONS: In obese T2DM patients, the 24hR performed reasonably well for probing nutrient intake before and after RYGB, but is not recommended for tracking changes over time, including pre- versus postoperative deficits.

Introducción: Las evaluaciones nutricionales y de ingesta de comida antes y después de cirugía bariátrica puede ser importante para corregir hábitos de alimentación y deficiencias nutricionales. Objetivo: Evaluar la eficacia del formulario de recordatorio de alimento de 24 horas (24hR) como método rápido de evaluación de ingesta de nutrientes antes y después de bypass gástrico en Y de Roux (RYGB). Métodos: Este estudio analizó datos de 10 pacientes obesos con diabetes mellitus de tipo 2 (T2DM). Se evaluó la ingesta de alimentos (calorías, macronutrientes y micronutrientes) antes y 3 meses después de RYGB con el 24hR además del registro de alimentos de siete días (7dR) como patrón de referencia estándar. Se utilizó el software Virtual Nutri Plus® para cuantificar los nutrientes. Resultados: Los datos del 7dR revelaron deficiencias en la ingesta estimada de fibra total y 14 de 22 micronutrientes pre- y postoperación, combinados; el 24hR no pudo detectar deficiencias de ingesta en solo dos de estos micronutrientes (vitaminas A y B3). Otras deficiencias postoperatorias incluyeron carbohidratos, vitamina B1, cobre y hierro, sobre las que el 24hR detectó solo la deficiencia de hierro. En nuestros análisis comparativos pre- y post-operación, el 7dR reveló descensos en calorías totales, carbohidratos, grasas totales y todas las subclasses de grasa y fibra, y 12 micronutrientes; en los análisis comparativos análogos, el 24hR no logró detectar descensos en la ingesta de grasas monoinsaturadas y saturadas y en seis de estos micronutrientes. Conclusiones: En pacientes obesos con T2DM, el 24hR funcionó razonablemente bien demostrando la ingesta de nutrientes antes y después de RYGB, pero no se recomienda para registrar cambios a lo largo del tiempo, incluyendo deficiencias pre- y postoperación.