Effect of increasing doses of chitosan to grazing beef steers on the relative population and transcript abundance of Archaea and cellulolytic and amylolytic bacterias.

This paper aims to investigate the influence of increasing chitosan doses on the relative proportion and abundance of cellulotytic, amylolytic bacteria, and Archaea transcripts for grazing cattle. Five rumen cannulated crossbread steers [3.6 months and 300 ± 25 kg body LW (live weight), mean ± standard deviation] were used in a 5 × 5 latin square design, randomly assigned to treatment sequence containing chitosan added to 0, 400, 800, 1200, or 1600 mg/kg concentrate. There was the effect of chitosan on the population of Fibrobacter succinogenes, Ruminococcus albus, and Archaea. The lowest population of these bacteria of 576.60 mg/kg DM (dry matter), 1010.40 mg/kg DM, and 634.80 mg/kg DM were noted when chitosan was added at levels of 3.87, 4.16, and 3.52. Except for Ruminococcus albus, which was not affected by increasing chitosan doses, supplementation of this additive in the concentrate quadratically increased the relative abundance of Fibrobacter succinogenes and Archaea Supplemental 740 mg CHI/kg concentrate for grazing steers receiving concentrate at 150 grams/100 kg LW is recommended to promote minimal effect on the relative population and abundance of cellulolytics and amylomatics and to restrict Archaea growth.

Effect of crude glycerin levels on meat quality and carcass characteristics of crossbred Boer goats.

This study assessed the effects of four levels of crude glycerin (0, 50, 100, and 150 g/kg dry matter (DM) basis) in the diet of Boer crossbred goat kids on the qualitative and quantitative carcass characteristics as well as meat quality. Thirty-two crossbred, castrated Boer x undefined breed goat kids with an initial average weight of 17.8 ± 2.2 kg between 3 and 4 months of age were distributed across a complete randomized experiment with four treatments and eight replicates. The DM intake linearly decreased (p < .05) as the crude glycerin inclusion level in the diet increased. Crude glycerin levels decreased (linear effect, p < .05) empty body weight, hot dressing percentage, and cold dressing percentage. Conformation and subcutaneous fat thickness were not affected (p > .05) by dietary crude glycerin. Crude glycerin levels decreased (linear effect, p = .03) rib eye area of the Longissimus dorsi muscle, however, did not affect color, cooking loss, and shear force. The crude glycerin can be included up to 50 g/kg DM in the diet of crossbred Boer goats without negatively affecting carcass characteristics and meat quality. It can be recommended as an energy source in finishing diets.

Nutrient intake, digestibility, feeding behavior, nitrogen balance, and performance of feedlot goat kids fed high-concentrate diets containing licury cake (Syagrus coronata).

The current trial was carried out to evaluate the impacts of adding licury cake (Syagrus coronata) in high-concentrate diets for goat kids feedlot-finished on growth performance and metabolic parameters. Forty crossbred non-castrated Boer goat kids with approximately four months and similar initial live weights (20.15 ± 2.79 kg). The experimental design used in this study was a completely randomized, with four diets (0, 10, 20, and 30% of licury cake inclusion in a dry matter basis (DM)). Licury cake inclusion decreased nutrient intake and digestibility (P < 0.05). Times spent in feeding (P < 0.001) and rumination (P = 0.003) activities, number of events in feeding (P < 0.001) and idling (P = 0.015) increased, whereas a linear reduction occurred in feeding and rumination efficiencies of DM and neutral detergent fiber (P < 0.001). Nitrogen (N) intake, retained N (P < 0.001), triglycerides concentrations (P = 0.002), aspartate-aminotransferase, alanine-aminotransferase, and gamma-glutamyltransferase decreased with the licury cake inclusion (P < 0.05). Final body weight (P = 0.008), average daily gain (P = 0.001), and total weight gain (P = 0.001) decreased linearly when licury cake was included in diets. High-concentrate diets containing increasing levels of licury cake decrease intake and apparent digestibility of nutrients and performance when supplied for goat kids feedlot-finished. Therefore, the inclusion of the studied levels of licury cake in goat kids fed high-concentrate diets is not recommended for feedlot-finished systems.

Effect of selenium and vitamin E supplementation on the metabolic status of dairy goats and respective goat kids in the peripartum period.

This study aimed to examine the effects of selenium (Se) and vitamin E (vitE) supplementation on blood cell counts and blood metabolite concentrations in goats and their kids. Fifteen Saanen goats (average age 6 years of age; average initial body weight of 70 ± 10 kg) and 21 ½ Saanen × ½ Pardo Alpine crossbred goat kids (average body weight of 3.70 ± 0.64 kg) were used. Animals were distributed in a completely randomized design with five replicates per diet for mother goats and seven for goat kids and randomly assigned into three groups in the following diets: CON, control basal diet; Se, inclusion of 3.2 mg of Se/kg DM; SevitE, inclusion of 3.2 mg Se/kg DM and 1145 IU/day vitE/kg DM. Effects of time were observed on red blood cells, hemoglobin, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin in goats and goat kids. Effects of time were observed on differential counts of leucocytes, lymphocytes, and monocytes in goat kids. Interaction was observed for high-density lipoprotein and total protein in goats and for triglycerides, beta-hydroxybutyrate (BHBA), and gamma-glutamyltransferase (GGT) in goat kids. Effects of time were observed on low-density lipoprotein, triglycerides, glucose, lactate, BHBA, non-esterified fatty acids (NEFA), creatinine, aspartate-aminotransferase, and GGT in goats and all blood metabolites in goat kids. Selenium, vitE, or association in the evaluated levels are not sufficient to change blood cell counts when supplied in diets for goats or goat kids. However, the effect of time or interaction between time and diets change the blood metabolite concentrations in the animals.

Palm kernel cake in high-concentrate diets for feedlot goat kids: nutrient intake, digestibility, feeding behavior, nitrogen balance, blood metabolites, and performance.

This study was conducted to evaluate the effects of including palm kernel cake (PKC) in high-concentrate diets for feedlot goat kids on nutrient intake, digestibility, feeding behavior, nitrogen balance, blood metabolites, and performance. Thirty-two castrated crossbred Boer × mixed breed goat kids with an average age of 4 months and an initial body weight of 19.65 ± 3.00 kg were distributed in a completely randomized design. The diets included one of four levels (0, 12, 24, and 36%) of PKC on a total dry matter basis. The PKC inclusion in the diets promoted quadratic effects in the nutrient intakes (P ≤ 0.05). The digestibility of ether extract increased (P = 0.010), whereas the digestibility of non-fibrous carbohydrates decreased (P = 0.017) with the inclusion of PKC. Palm kernel cake inclusion promoted a quadratic effect on the time spent per episode feeding and decreased the times spent idling and ruminated bolus per day (P ≤ 0.05). The ingested and retained nitrogen decreased with the inclusion of PKC (P ≤ 0.05). The inclusion of PKC in the diets had quadratic effects in the cholesterol concentrations, albumin (A), globulin (G), A:G ratio, and gamma-glutamyltransferase enzyme activity (P ≤ 0.05). The PKC inclusion promoted a quadratic increase in total weight gain (P = 0.026), with the highest value achieved at the inclusion level of 11.68%. The inclusion of up to 12% PKC in high-concentrate diets increases the intake and growth performance without compromising the digestibility of nutrients and feed efficiency.

Estimation of ruminal outflow in buffaloes fed diets with different energy and protein sources by use of reticular and omasal sampling.

This study aimed to evaluate the effects of different traditional or alternative energy and protein sources, associated or not, on feeding behavior, ruminal kinetics, and post-ruminal flow of nutrients. Besides, it was assessed diets' effects on different sites (reticulum and omasum) of buffaloes. Four ruminally cannulated male Murrah buffaloes (average initial weight of 637 ± 66.37 kg) were randomly distributed in a 4 × 4 Latin square design. Treatments were arranged as 2 × 2 factorial arrangement. The first factor evaluated was the inclusion of energy sources (ground corn and crude glycerin), and the second factor was the inclusion of protein sources (soybean meal and cottonseed cake). Buffaloes fed cottonseed cake had a higher content of neutral detergent fiber (NDF) and potentially digestible detergent fiber (pdNDF) in the rumen environment than buffaloes fed soybean meal. There was a sampling site effect on rumen digestion rates of pdNDF, passage rates of indigestible neutral detergent fiber (iNDF), and pdNDF, and flow of iNDF. In this study, omasal collections were more representative. Total replacement of ground corn by crude glycerin promoted less NDF ruminal digestibility, and care should be taken to include this energy source. The cottonseed cake does not cause a difference in rumen dynamics and can totally replace soybean meal in feedlot buffaloes' diet.

Digestion, ruminal metabolism, and feeding behavior of buffaloes fed diets supplemented with soybean oil, whole and raw soybean, and calcium salts of fatty acids.

This study aimed to evaluate the effects of the inclusion of unsaturated fatty acid (UFA) sources on the nutrient intake, apparent digestibility, ruminal fermentation, and feeding behavior in diets for buffaloes. Four castrated Murrah buffaloes with approximately 24 months of age and an initial average body weight of 351 ± 15 kg were randomly assigned to a 4 × 4 Latin square experiment, containing the following diets: (1) control (CON): control diet based on soybean meal and ground corn, (2) soybean oil (SO): dietary inclusion of 2.20% (DM basis), (3) whole raw soybean (WRS): dietary inclusion of 16.0%, and (4) calcium salts of FA (CSFA): dietary inclusion of 2.60%. There was an effect of diets in ether extract intake among buffaloes fed UFA and CON diets, and among buffaloes fed CSFA and WRS diets (P < 0.05). Diets containing UFA sources provided higher EE digestibility (P < 0.05). Buffaloes fed WRS had higher rumen pH values than animals fed the CSFA diet (P < 0.05). Supplementation of UFA sources decreased the molar concentrations of short-chain fatty acids (P < 0.05). Diets influenced the times spent in chewing, idling, and the rumination efficiencies of DM and NDF (P < 0.05). The supplementation with WRS, SO, and CSFA does not negatively affect intake, digestion, ruminal metabolism, and feeding behavior. The WRS as a fat supplement source decreases dietary costs by replacing ground corn and soybean meal simultaneously compared to other fat sources used. Nevertheless, whole and raw soybean in buffaloes' diet can reduce chewing and rumination activity.

Assessment of the metabolic, protein, energy, and liver profiles of lambs finished in a feedlot and receiving diets containing groundnut cake.

Forty-five crossbred Dorper × Santa Inês lambs were selected to assess the effect of replacing soybean meal by groundnut cake metabolic profile and histopathological evaluation of the kidney and liver tissues. The animals were 5 months old on average with a 24.5 ± 5.3 kg initial body weight which were maintained fed in feedlot with groundnut cake (0, 25, 50, 75, and 100%) as the replacement for soybean meal. Confinement lasted 84 days, and on the last day, the animals were fasted and slaughtered. A completely randomized design with five treatments and nine repetitions was used. Blood samples were collected from the jugular vein of each animal to evaluate the metabolic, protein, energy, and liver profiles of lambs. No effect was observed on protein, energy, or enzymatic profiles, which remained within the normal range for sheep. A histopathological examination was performed, and no deleterious effects from groundnut cake were observed in the liver or kidney tissues. Groundnut cake as a replacement for soybean meal can be used as an alternative protein source up to 100% because it does not affect the metabolic, protein, and energy profiles or the liver and kidney functions of the growing sheep for slaughter.