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1.
Sci Adv ; 10(4): eadk1033, 2024 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-38277456

RESUMO

Records of past societies confronted with natural climate change can illuminate social responses to environmental stress and environment-disease connections, especially when locally constrained high-temporal resolution paleoclimate reconstructions are available. We present a temperature and precipitation reconstruction for ~200 BCE to ~600 CE, from a southern Italian marine sedimentary archive-the first high-resolution (~3 years) climate record from the heartland of the Roman Empire, stretching from the so-called Roman Climate Optimum to the Late Antique Little Ice Age. We document phases of instability and cooling from ~100 CE onward but more notably after ~130 CE. Pronounced cold phases between ~160 to 180 CE, ~245 to 275 CE, and after ~530 CE associate with pandemic disease, suggesting that climate stress interacted with social and biological variables. The importance of environment-disease dynamics in past civilizations underscores the need to incorporate health in risk assessments of climate change.


Assuntos
Mudança Climática , Pandemias , Itália/epidemiologia , Civilização , Temperatura
2.
Data Brief ; 18: 1900-1906, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29904694

RESUMO

A high-resolution analysis of the distribution of major and trace elements across the Cretaceous/Paleogene boundary (KPgB) in the distal section of Agost (SE Spain) was performed. The KPgB sediments were drilled to recover a 22 cm-long core; the lower 5 cm corresponding to the uppermost Maastrichtian and the upper 17 cm to the lowermost Danian. The unconsolidated sediments were resin-embedded under O2-free conditions, cut and polished. Laser Ablation-Inductivity Coupled Plasma-Mass Spectrometry (LA-ICP-MS) analyses were conducted at 10 µm increments and a laser-beam of 80 µm. Discrete samples were taken immediately prior to the resin-embedding and analyzed by Inductivity Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). Results obtained by both analytical methods (LA-ICP-MS and ICP-OES) are presented. (Further interpretations and discussion are included in Sosa-Montes de Oca et al., 2018 [6]).

3.
ISME J ; 12(6): 1414-1426, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29666446

RESUMO

The Kryos Basin is a deep-sea hypersaline anoxic basin (DHAB) located in the Eastern Mediterranean Sea (34.98°N 22.04°E). It is filled with brine of re-dissolved Messinian evaporites and is nearly saturated with MgCl2-equivalents, which makes this habitat extremely challenging for life. The strong density difference between the anoxic brine and the overlying oxic Mediterranean seawater impedes mixing, giving rise to a narrow chemocline. Here, we investigate the microbial community structure and activities across the seawater-brine interface using a combined biogeochemical, next-generation sequencing, and lipid biomarker approach. Within the interface, we detected fatty acids that were distinctly 13C-enriched when compared to other fatty acids. These likely originated from sulfide-oxidizing bacteria that fix carbon via the reverse tricarboxylic acid cycle. In the lower part of the interface, we also measured elevated rates of methane oxidation, probably mediated by aerobic methanotrophs under micro-oxic conditions. Sulfate reduction rates increased across the interface and were highest within the brine, providing first evidence that sulfate reducers (likely Desulfovermiculus and Desulfobacula) thrive in the Kryos Basin at a water activity of only ~0.4 Aw. Our results demonstrate that a highly specialized microbial community in the Kryos Basin has adapted to the poly-extreme conditions of a DHAB with nearly saturated MgCl2 brine, extending the known environmental range where microbial life can persist.


Assuntos
Bactérias , Cloreto de Magnésio/química , Microbiota , Sais/química , Água do Mar/microbiologia , Microbiologia da Água , Biomarcadores/metabolismo , Lipídeos/química , Mar Mediterrâneo , Oxigênio/química , Filogenia , RNA Ribossômico 16S/química , Sulfatos/química , Sulfetos/química
4.
Rev Sci Instrum ; 87(4): 043103, 2016 04.
Artigo em Inglês | MEDLINE | ID: mdl-27131650

RESUMO

The next generation of far infrared space observatories will require extremely sensitive detectors that can be realized only by combining extremely low intrinsic noise with high optical efficiency. We have measured the broad-band optical response of ultra-sensitive transtion edge sensor (TES) bolometers (NEP≈2aW/Hz) in the 30-60-µm band where radiation is coupled to the detectors with a few-moded conical feedhorn and a hemispherical backshort. We show that these detectors have an optical efficiency of 60% (the ratio of the power detected by the TES bolometer to the total power propagating through the feedhorn). We find that the measured optical efficiency can be understood in terms of the modes propagating through the feedhorn with the aid of a spatial mode-filtering technique.

5.
Artigo em Inglês | MEDLINE | ID: mdl-24182597

RESUMO

OBJECTIVE: The gain of mineralized bone was compared between deproteinized bovine bone allograft (DBA) and biphasic calcium phosphate (BCP) for dental implant placement. STUDY DESIGN: Five patients with atrophic maxillae underwent bilateral sinus elevation with DBA (Bio-Oss) and BCP (Straumann BoneCeramic). After 3 to 8 months, 32 Camlog implants were placed, and biopsies were retrieved. Bone and graft volume, degree of bone mineralization, and graft degradation gradient were determined using micro-computed tomography, and bone formation and resorption parameters were measured using histomorphometry. Implant functioning and peri-implant mucosa were evaluated up to 4 years. RESULTS: Patients were prosthetically successfully restored. All but one of the implants survived, and peri-implant mucosa showed healthy appearance and stability. Bone volume, graft volume, degree of bone mineralization, and osteoclast and osteocyte numbers were similar, but BCP-grafted biopsies had relatively more osteoid than DBA-grafted biopsies. CONCLUSIONS: The BCP and DBA materials showed similar osteoconductive patterns and mineralized bone, although signs of more active bone formation and remodeling were observed in BCP- than in DBA-grafted biopsies.


Assuntos
Regeneração Óssea/efeitos dos fármacos , Substitutos Ósseos/farmacologia , Transplante Ósseo/métodos , Fosfatos de Cálcio/farmacologia , Seio Maxilar/fisiopatologia , Idoso , Análise de Variância , Animais , Regeneração Óssea/fisiologia , Bovinos , Implantação Dentária Endóssea , Feminino , Seguimentos , Técnicas Histológicas , Humanos , Masculino , Seio Maxilar/diagnóstico por imagem , Seio Maxilar/cirurgia , Pessoa de Meia-Idade , Microtomografia por Raio-X/métodos
6.
Extremophiles ; 17(2): 289-99, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23340764

RESUMO

To extend the knowledge of anaerobic ammonium oxidation (anammox) habitats, bacterial communities were examined in two hypersaline sulphidic basins in Eastern Mediterranean Sea. The 2 m thick seawater-brine haloclines of the deep anoxic hypersaline basins Bannock and L'Atalante were sampled in intervals of 10 cm with increasing salinity. (15)N isotope pairing incubation experiments showed the production of (29)N2 and (30)N2 gases in the chemoclines, ranging from 6.0 to 9.2 % salinity of the L'Atalante basin. Potential anammox rates ranged from 2.52 to 49.65 nmol N2 L(-1) day(-1) while denitrification was a major N2 production pathway, accounting for more than 85.5 % of total N2 production. Anammox-related 16S rRNA genes were detected along the L'Atalante and Bannock haloclines up to 24 % salinity, and the amplification of the hydrazine synthase genes (hzsA) further confirmed the presence of anammox bacteria in Bannock. Fluorescence in situ hybridisation and sequence analysis of 16S rRNA genes identified representatives of the marine anammox genus 'Candidatus Scalindua' and putatively new operational taxonomic units closely affiliated to sequences retrieved in marine environments that have documented anammox activity. 'Scalindua brodae' like sequences constituted up to 84.4 % of the sequences retrieved from Bannock. The anammox community in L'Atalante was different than in Bannock and was stratified according to salinity increase. This study putatively extends anammox bacterial habitats to extremely saline sulphidic ecosystems.


Assuntos
Amônia/metabolismo , Bactérias Anaeróbias/isolamento & purificação , Água do Mar/microbiologia , Anaerobiose , Bactérias Anaeróbias/classificação , Bactérias Anaeróbias/genética , Bactérias Anaeróbias/metabolismo , Genes Bacterianos , Genes de RNAr , Hidrazinas/metabolismo , Mar Mediterrâneo , Isótopos de Nitrogênio , Oxirredução , Filogenia , Salinidade , Análise de Sequência de DNA
7.
Artigo em Inglês | MEDLINE | ID: mdl-23246225

RESUMO

OBJECTIVE: To assess the predictors of implant failure after grafted maxillary sinus (GMS). MATERIAL AND METHODS: A total of 1045 implants were inserted in 224 patients/347 GMS during a period of 14 years. Kaplan-Meyer and multivariate log-regression analysis were used to assess the following variates: patient's age, gender, smoker/nonsmoker, American Society of Anesthesiologists (ASA) class, one/two-stage surgery, merged/submerged healing, membrane, antibiotics, auto/allo/xenogenic bone grafts, implant's lengths/surface/diameter, crestal bone atrophy/quality, implant region, prosthetics, opposing dentition, and implant proximity to evaluate the predictors and relative risk (hazard ratio [HR]) of implant failure. RESULTS: Significant implant failure predictors were the graft material (HR = 4.7), with superior results for autogenic bone, residual crestal bone height (HR = 3.51), ASA class (HR = 2.73), surgical technique (HR = 2.56), implant proximity (HR = 2.07), smoker (HR = 1.98), and age (>60/HR=1.39). All other factors were insignificant. Overall survival rate was 93.3%. CONCLUSIONS: GMS is effective when the predictors are considered. Patient selection, including the ASA status, smoking, residual bone height, and the graft material are the predominant predictors. In highly atrophic situations, autogenic bone grafts showed superiority; however, in less atrophic cases, nonautogenic bone-grafts are equivalent.


Assuntos
Implantes Dentários/estatística & dados numéricos , Falha de Restauração Dentária/estatística & dados numéricos , Procedimentos de Cirurgia Plástica/métodos , Levantamento do Assoalho do Seio Maxilar/métodos , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Antibioticoprofilaxia/estatística & dados numéricos , Atrofia , Densidade Óssea/fisiologia , Substitutos Ósseos/uso terapêutico , Transplante Ósseo/métodos , Implantação Dentária Endóssea/efeitos adversos , Implantação Dentária Endóssea/métodos , Implantação Dentária Endóssea/estatística & dados numéricos , Planejamento de Prótese Dentária , Feminino , Seguimentos , Previsões , Nível de Saúde , Humanos , Masculino , Maxila/patologia , Membranas Artificiais , Pessoa de Meia-Idade , Procedimentos de Cirurgia Plástica/efeitos adversos , Procedimentos de Cirurgia Plástica/estatística & dados numéricos , Estudos Retrospectivos , Fatores de Risco , Fatores Sexuais , Levantamento do Assoalho do Seio Maxilar/efeitos adversos , Levantamento do Assoalho do Seio Maxilar/estatística & dados numéricos , Fumar , Análise de Sobrevida
9.
Microb Ecol ; 62(3): 655-68, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21538105

RESUMO

We investigated the top 30-cm sediment prokaryotic community structure in 5-cm spatial resolution, at an active site of the Amsterdam mud volcano, East Mediterranean Sea, based on the 16S rRNA gene diversity. A total of 339 and 526 sequences were retrieved, corresponding to 25 and 213 unique (≥98% similarity) phylotypes of Archaea and Bacteria, respectively, in all depths. The Shannon-Wiener diversity index H was higher for Bacteria (1.92-4.03) than for Archaea (0.99-1.91) and varied differently between the two groups. Archaea were dominated by anaerobic methanotrophs ANME-1, -2 and -3 groups and were related to phylotypes involved in anaerobic oxidation of methane from similar habitats. The much more complex Bacteria community consisted of 20 phylogenetic groups at the phylum/candidate division level. Proteobacteria, in particular δ-Proteobacteria, was the dominant group. In most sediment layers, the dominant phylotypes of both the Archaea and Bacteria communities were found in neighbouring layers, suggesting some overlap in species richness. The similarity of certain prokaryotic communities was also depicted by using four different similarity indices. The direct comparison of the retrieved phylotypes with those from the Kazan mud volcano of the same field revealed that 40.0% of the Archaea and 16.9% of the Bacteria phylotypes are common between the two systems. The majority of these phylotypes are closely related to phylotypes originating from other mud volcanoes, implying a degree of endemicity in these systems.


Assuntos
Archaea/isolamento & purificação , Bactérias/isolamento & purificação , Biodiversidade , Sedimentos Geológicos/microbiologia , Archaea/genética , Archaea/crescimento & desenvolvimento , Bactérias/genética , Bactérias/crescimento & desenvolvimento , DNA Arqueal/genética , DNA Bacteriano/genética , Sedimentos Geológicos/química , Mar Mediterrâneo , Filogenia , RNA Ribossômico 16S/genética , Água do Mar/química , Água do Mar/microbiologia , Análise de Sequência de DNA , Microbiologia da Água
10.
Clin Oral Implants Res ; 20(11): 1231-9, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19709060

RESUMO

OBJECTIVES: Although implants with a roughened surface are widely used today, little is known about the long-term effect of a roughened surface compared with the conventional machined surface on clinical and radiographic parameters. The purpose of this study is to investigate the long-term differences between moderately rough (tioblasted) titanium implants and minimally rough machined/turned surfaces with respect to marginal bone resorption and the peri-implant soft tissues in the same patient. MATERIAL AND METHODS: In 20 fully edentulous patients, with severely resorbed mandibles, a total of 80 Astra Tech dental implants were placed in the mandible to support a bar construction with a full overdenture. In each patient two minimally rough-surfaced (turned) and two moderately rough-surfaced (tioblast) implants were placed alternately. Clinical evaluation was carried out at base line (prosthetic installation), 6 months, 1, 2, 3, 4, 5 and 12 years. Radiographic evaluation using standardized individual filmholders was carried out at base line (prosthetic installation), 6 months, 1, 5 and 12 years. RESULTS: In two patients, during the abutment surgery, one turned implant showed insufficient osseointegration and was replaced. One implant showed an abutment fracture after 9 years and was kept as a sleeper. From base line up to 12 years, no implant was lost. No significant differences were found between both implant surfaces concerning the clinical parameters such as plaque, calculus, bleeding and probing pocket depth. The mean (SD) marginal bone changes up to 12 years varied between -0.11 and +0.01 mm for the turned and -0.2 and +0.01 mm for the tioblast implants. No significant difference in marginal bone loss was found between both implant surfaces. CONCLUSION: We conclude that after 12 years of follow-up, no differences could be found between the turned and the tioblasted implants, both for soft and for hard tissue parameters.


Assuntos
Dente Suporte , Implantes Dentários , Planejamento de Prótese Dentária , Prótese Dentária Fixada por Implante , Arcada Edêntula/reabilitação , Adulto , Idoso , Perda do Osso Alveolar/complicações , Dente Suporte/efeitos adversos , Implantes Dentários/efeitos adversos , Índice de Placa Dentária , Falha de Restauração Dentária , Retenção de Dentadura/instrumentação , Prótese Total Inferior , Revestimento de Dentadura , Método Duplo-Cego , Feminino , Humanos , Análise de Intenção de Tratamento , Arcada Edêntula/complicações , Estudos Longitudinais , Masculino , Mandíbula/diagnóstico por imagem , Pessoa de Meia-Idade , Osseointegração , Índice Periodontal , Radiografia , Estatísticas não Paramétricas , Propriedades de Superfície , Titânio
11.
Proc Natl Acad Sci U S A ; 106(23): 9151-6, 2009 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-19470485

RESUMO

Urania basin in the deep Mediterranean Sea houses a lake that is >100 m deep, devoid of oxygen, 6 times more saline than seawater, and has very high levels of methane and particularly sulfide (up to 16 mM), making it among the most sulfidic water bodies on Earth. Along the depth profile there are 2 chemoclines, a steep one with the overlying oxic seawater, and another between anoxic brines of different density, where gradients of salinity, electron donors and acceptors occur. To identify and differentiate the microbes and processes contributing to the turnover of organic matter and sulfide along the water column, these chemoclines were sampled at a high resolution. Bacterial cell numbers increased up to a hundredfold in the chemoclines as a consequence of elevated nutrient availability, with higher numbers in the upper interface where redox gradient was steeper. Bacterial and archaeal communities, analyzed by DNA fingerprinting, 16S rRNA gene libraries, activity measurements, and cultivation, were highly stratified and metabolically more active along the chemoclines compared with seawater or the uniformly hypersaline brines. Detailed analysis of 16S rRNA gene sequences revealed that in both chemoclines delta- and epsilon-Proteobacteria, predominantly sulfate reducers and sulfur oxidizers, respectively, were the dominant bacteria. In the deepest layers of the basin MSBL1, putatively responsible for methanogenesis, dominated among archaea. The data suggest that the complex microbial community is adapted to the basin's extreme chemistry, and the elevated biomass is driven largely by sulfur cycling and methanogenesis.


Assuntos
Archaea/metabolismo , Bactérias/metabolismo , Água do Mar/microbiologia , Enxofre/metabolismo , Ecossistema , Manganês/metabolismo , Dados de Sequência Molecular , Nitratos/metabolismo , Oxigênio/metabolismo , Salinidade , Água/metabolismo
12.
Appl Environ Microbiol ; 74(10): 3198-215, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18378658

RESUMO

In this study we determined the composition and biogeochemistry of novel, brightly colored, white and orange microbial mats at the surface of a brine seep at the outer rim of the Chefren mud volcano. These mats were interspersed with one another, but their underlying sediment biogeochemistries differed considerably. Microscopy revealed that the white mats were granules composed of elemental S filaments, similar to those produced by the sulfide-oxidizing epsilonproteobacterium "Candidatus Arcobacter sulfidicus." Fluorescence in situ hybridization indicated that microorganisms targeted by a "Ca. Arcobacter sulfidicus"-specific oligonucleotide probe constituted up to 24% of the total the cells within these mats. Several 16S rRNA gene sequences from organisms closely related to "Ca. Arcobacter sulfidicus" were identified. In contrast, the orange mat consisted mostly of bright orange flakes composed of empty Fe(III) (hydr)oxide-coated microbial sheaths, similar to those produced by the neutrophilic Fe(II)-oxidizing betaproteobacterium Leptothrix ochracea. None of the 16S rRNA gene sequences obtained from these samples were closely related to sequences of known neutrophilic aerobic Fe(II)-oxidizing bacteria. The sediments below both types of mats showed relatively high sulfate reduction rates (300 nmol x cm(-3) x day(-1)) partially fueled by the anaerobic oxidation of methane (10 to 20 nmol x cm(-3) x day(-1)). Free sulfide produced below the white mat was depleted by sulfide oxidation within the mat itself. Below the orange mat free Fe(II) reached the surface layer and was depleted in part by microbial Fe(II) oxidation. Both mats and the sediments underneath them hosted very diverse microbial communities and contained mineral precipitates, most likely due to differences in fluid flow patterns.


Assuntos
Bactérias/classificação , Bactérias/metabolismo , Biodiversidade , Sedimentos Geológicos/microbiologia , Ferro/metabolismo , Enxofre/metabolismo , Arcobacter/citologia , Arcobacter/genética , Bactérias/genética , Bactérias/isolamento & purificação , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Compostos Férricos/metabolismo , Compostos Ferrosos/metabolismo , Genes de RNAr , Leptothrix/citologia , Dados de Sequência Molecular , Oxirredução , Filogenia , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , Sulfetos/metabolismo
13.
Nature ; 440(7081): 203-7, 2006 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-16525471

RESUMO

The chemical composition of the Bannock basin has been studied in some detail. We recently showed that unusual microbial populations, including a new division of Archaea (MSBL1), inhabit the NaCl-rich hypersaline brine. High salinities tend to reduce biodiversity, but when brines come into contact with fresher water the natural haloclines formed frequently contain gradients of other chemicals, including permutations of electron donors and acceptors, that may enhance microbial diversity, activity and biogeochemical cycling. Here we report a 2.5-m-thick chemocline with a steep NaCl gradient at 3.3 km within the water column betweeen Bannock anoxic hypersaline brine and overlying sea water. The chemocline supports some of the most biomass-rich and active microbial communities in the deep sea, dominated by Bacteria rather than Archaea, and including four major new divisions of Bacteria. Significantly higher metabolic activities were measured in the chemocline than in the overlying sea water and underlying brine; functional analyses indicate that a range of biological processes is likely to occur in the chemocline. Many prokaryotic taxa, including the phylogenetically new groups, were confined to defined salinities, and collectively formed a diverse, sharply stratified, deep-sea ecosystem with sufficient biomass to potentially contribute to organic geological deposits.


Assuntos
Archaea/metabolismo , Bactérias/metabolismo , Ecossistema , Oxigênio/metabolismo , Células Procarióticas/metabolismo , Água do Mar/microbiologia , Microbiologia da Água , Aerobiose , Anaerobiose , Archaea/classificação , Archaea/genética , Archaea/isolamento & purificação , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Biodiversidade , Dados de Sequência Molecular , Oceanos e Mares , Células Procarióticas/classificação , Navios
14.
Science ; 307(5706): 121-3, 2005 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-15637281

RESUMO

Deep hypersaline anoxic basins in the Mediterranean Sea are a legacy of dissolution of ancient subterranean salt deposits from the Miocene period. Our study revealed that these hypersaline basins are not biogeochemical dead ends, but support in situ sulfate reduction, methanogenesis, and heterotrophic activity. A wide diversity of prokaryotes was observed, including a new, abundant, deeply branching order within the Euryarchaeota. Furthermore, we demonstrated the presence of a unique, metabolically active microbial community in the Discovery basin, which is one of the most extreme terrestrial saline environments known, as it is almost saturated with MgCl2 (5 M).


Assuntos
Archaea/fisiologia , Fenômenos Fisiológicos Bacterianos , Ecossistema , Água do Mar/microbiologia , Cloreto de Sódio , Anaerobiose , Archaea/classificação , Archaea/isolamento & purificação , Bactérias/classificação , Bactérias/isolamento & purificação , Biodiversidade , Análise por Conglomerados , Euryarchaeota/classificação , Euryarchaeota/isolamento & purificação , Euryarchaeota/fisiologia , Genes Arqueais , Genes Bacterianos , Genes de RNAr , Cloreto de Magnésio/análise , Mar Mediterrâneo , Metano/metabolismo , Dados de Sequência Molecular , Oxirredução , Filogenia , RNA Ribossômico 16S/genética , Água do Mar/química , Sulfatos/metabolismo
15.
Biomaterials ; 26(12): 1445-51, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15482833

RESUMO

We and others have shown earlier that porous beta-tricalcium phosphate (TCP) (Cerasorb) can be used in patients to augment the maxillary sinus floor prior to placement of oral dental implants. To better understand the transformation of TCP particles into bone tissue, we analyse here the appearance of cells with osteogenic or osteoclastic potential in relation to these particles. In biopsies taken at 6 months after sinus floor augmentation we observed bone growth into the TCP particles but also replacement by soft connective tissue. To identify possible osteoprogenitor cells in this tissue, histological sections were immunostained with an antibody to Runx2/Cbfa1, an essential and early transcription factor for osteoblast differentiation. The osteogenic potential of cells was further confirmed by immunostaining for bone sialoprotein (BSP) and osteopontin (OPN). Other sections were stained for Tartrate Resistant Acid Phosphatase (TRAP) activity to identify cells with osteoclastic capacity. Runx2/Cbfa1 positive connective tissue cells were found in abundance throughout and around the TCP particles, even at a distance of several millimetres from the maxillary bone surface. About 95% of the cells found within TCP particles stained positive for Runx2/Cbfa1. Fewer cells stained positive for BSP and OPN, suggesting more mature osteoblastic properties. Mono- and binucleate TRAP-positive cells, but no multinucleate TRAP-positive osteoclasts, were found in the soft tissue infiltrating the TCP and at the surface of the TCP particles. Both the Runx2/Cbfa1 positive and the TRAP-positive cells decreased apically with increasing vertical distance from the maxillary bone surface. This data suggests that the TCP particles attract osteoprogenitor cells that migrate into the interconnecting micropores of the bone substitute material by 6 months. The lack of large multinucleate TRAP positive cells suggests that resorption of the TCP material by osteoclasts plays only a minor role in its replacement by bone. Chemical dissolution, possibly favoured by a high cell metabolism in the particles, seems the predominant cause of TCP degradation. The abundance of Runx2/Cbfa1 positive cells would indicate that with a greater time of healing there will be further bone deposition into these particles.


Assuntos
Fosfatos de Cálcio/uso terapêutico , Regeneração Tecidual Guiada Periodontal/métodos , Seio Maxilar/citologia , Seio Maxilar/cirurgia , Procedimentos Cirúrgicos Pré-Protéticos Bucais/métodos , Osteoblastos/citologia , Osteoclastos/citologia , Osteogênese/fisiologia , Substitutos Ósseos/química , Diferenciação Celular , Implantação Dentária Endóssea/métodos , Humanos , Seio Maxilar/efeitos dos fármacos , Osseointegração/fisiologia , Osteoblastos/fisiologia , Osteoclastos/fisiologia , Resultado do Tratamento
16.
Clin Oral Implants Res ; 15(6): 724-32, 2004 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-15533134

RESUMO

Tricalcium phosphate (TCP) has been historically a well-accepted material for bone augmentation. We examined the use of a porous beta-TCP (100%) in a split mouth model for sinus floor augmentation. Five patients were treated bilaterally, receiving 1-2 mm-sized beta-TCP particles (Cerasorb) on one side (test side) and autologous chin bone particles on the other (control) side. Four other patients were treated with a unilateral sinus floor augmentation using 100% beta-TCP (no controls). Biopsies of the augmented sites were taken at 6 months. Histomorphometry measurements were carried out in order to quantify bone augmentation at test and control sides. The average bone volume (BV) formed in the augmented sinus at the control side was 41% (32-56%) and 17% (9-27%) in the test side when all nine patients were included (statistically significant, P=0.04). When only the five bilateral patients were included, mean BV of the test side was 19% (13-27%), which was also significantly different from the control side (P=0.009). Osteoid formation tended to be higher in the test side biopsies (1.3%) than in the controls (0.3%) (marginally significant, P=0.1), indicating ongoing bone formation in the TCP material. The amount of lamellar bone at the test side was less than half the amount in the control side, indicating that remodelling had only recently started in the TCP-augmented side. The resorption surface, however, did not differ significantly between the two sides. These histological results indicate that Cerasorb is an acceptable bone substitute material for augmentation of the maxillary sinus. Due to the osteoconductive, but not osteoinductive properties of this material, the rate of bone formation is somewhat delayed in comparison to autologous bone.


Assuntos
Materiais Biocompatíveis/administração & dosagem , Fosfatos de Cálcio/administração & dosagem , Implantação Dentária/métodos , Seio Maxilar/cirurgia , Osteogênese/fisiologia , Adulto , Idoso , Materiais Biocompatíveis/uso terapêutico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Transplante Autólogo
17.
Clin Oral Implants Res ; 14(6): 759-66, 2003 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-15015953

RESUMO

Local bone defects in the anterior maxilla are commonly grafted with monocortical blocks of autologous bone in order to restore the defect site prior to the placement of dental implants. Increasing evidence suggests that osteocytes are involved in the control of bone remodelling and thus may be important for optimalisation of bone structure around implants, and thus for implant osseointegration. However, it is not well known whether osteocytes will survive when bone blocks are grafted into defects. We grafted 19 patients with monocortical bone blocks derived from the symphysis, to the defect site in the maxillary alveolar process. The bone grafts were left to heal for times varying from 2.5 to 7 months. During implant installation, bone biopsies were removed using a trephine burr, and processed for hard tissue histology. Bone histology and histomorphometry were then carried out in order to gain insight into the density, viability and remodelling of the graft. Clinically, all the bone grafts were successful, with no implant failures, and little resorption was seen. Histologically, bone volume expressed as percentage of tissue volume at the implant site varied from 27% to 57% with an overall average of 41%. Bone fields with empty osteocyte lacunae were observed and measured. The amount of this so-called nonvital bone (NVB) varied between 1% and 34% of the total tissue volume. The amount of NVB decreased significantly with the time of healing. The data suggest that the majority of the osteocytes of the monocortical bone do not survive grafting. The results indicate that the NVB is progressively remodelled into new vital bone 7 months after grafting.


Assuntos
Perda do Osso Alveolar/cirurgia , Aumento do Rebordo Alveolar , Transplante Ósseo , Sobrevivência de Enxerto/fisiologia , Doenças Maxilares/cirurgia , Adolescente , Adulto , Perda do Osso Alveolar/etiologia , Perda do Osso Alveolar/patologia , Biópsia , Sobrevivência Celular/fisiologia , Implantação Dentária Endóssea/métodos , Feminino , Seguimentos , Humanos , Masculino , Maxila/patologia , Maxila/cirurgia , Doenças Maxilares/complicações , Doenças Maxilares/patologia , Pessoa de Meia-Idade , Osteócitos/citologia
18.
Clin Oral Implants Res ; 13(4): 428-36, 2002 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12175381

RESUMO

In this study, high concentrations of bioactive glass (BG) particles were compared with autogenous bone in their capacity to augment maxillary bone when grafted in the human sinus floor using a split mouth design. Three female patients with severe maxillary atrophy underwent bilateral sinus floor elevation and bone grafting using 80-100% BG particles (300-355 microm in size) mixed with 20% to 0% iliac crest bone particles at one (experimental) side, and 100% iliac crest derived bone particles at the other (control) side. A total of 22 bone biopsies was taken at the time of fixture installation; that is, at 4, 6 and 15 months after grafting, and processed for histology and histomorphometry. At the control (autogenous bone) sides, trabecular bone amounted to 39% of the biopsy volume in the graft (site) at 4 months, almost 41% at 6 months, and 42% at 15 months. This bone contained viable osteocytes and was mostly of mature, lamellar type. At the experimental (BG particles) sides, the graft consisted of 27% of mostly woven (and some lamellar) bone at 4 months, 36% (woven and lamellar) bone at 6 months, and 39% (mainly lamellar) bone at 15 months. The grafted BG particles started to excavate at 4 months and their centers gradually filled with bone tissue. As a consequence, the volume of BG particles in the biopsy decreased from 29% at 4 months to 15% at 6 months and 8% at 15 months. The BG particles appeared to resorb within 1-2 years by dissolution rather than by osteoclastic activity. Parameters for bone turnover (% osteoid surface, % resorption surface) indicated that bone remodeling was very active at both experimental and control sides, during more than 6 months. These results suggest that mixtures of mainly (80-90%) BG particles and some (10-20%) autogenous bone are effective for bone regeneration in the augmented sinus offer 6 months healing time, while about 12 months healing time is needed for 100% BG particles.


Assuntos
Aumento do Rebordo Alveolar/métodos , Materiais Biocompatíveis/uso terapêutico , Substitutos Ósseos/uso terapêutico , Transplante Ósseo/métodos , Vidro , Maxila/cirurgia , Seio Maxilar/cirurgia , Implantes Absorvíveis , Idoso , Atrofia , Materiais Biocompatíveis/química , Biópsia , Matriz Óssea/patologia , Regeneração Óssea/fisiologia , Remodelação Óssea/fisiologia , Substitutos Ósseos/química , Transplante Ósseo/patologia , Implantes Dentários , Feminino , Seguimentos , Vidro/química , Humanos , Maxila/patologia , Seio Maxilar/patologia , Pessoa de Meia-Idade , Osteócitos/patologia , Solubilidade , Transplante Autólogo , Cicatrização/fisiologia
19.
Int J Periodontics Restorative Dent ; 22(3): 287-96, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12186351

RESUMO

A histomorphometric study was performed on six retrieved loaded hydroxyapatite (HA)-coated titanium implants. The implants had been clinically functioning in a 50-year-old patient. She originally presented a severely atrophic maxilla that was reconstructed by sinus floor augmentation using autogenous bone from the iliac crest. In spite of good clinical function, because of psychiatric problems, all implants, including some bordering bone, were removed using a trephine bur. Thin ground sections were prepared for histology and used for histomorphometry. The aim of this study was to observe the condition of the calcium phosphate coating after 2.5 years of functional loading, the nature of the bone-to-implant interface, as well as the density of the bone graft around the implants. Intimate and abundant bone-to-implant contact was observed, ranging from 90.4% to 99.8% along the implant surface. Active bone remodeling occurred within all threads, as demonstrated by secondary osteons close to the implant surface. The thickness of the calcium phosphate coating varied from 51 to 88 pm for the loaded retrieved implants, versus 53 to 89 microm for the nonused control implant. All implants showed bone contact including the first thread and up to the smooth titanium neck. Both the nature and thickness of the coating had hardly changed after 2.5 years of loading. The HA-coated implants used achieved excellent osseointegration and must be considered clinically safe and effective in maxillary grafted bone.


Assuntos
Materiais Revestidos Biocompatíveis , Implantes Dentários , Durapatita , Osseointegração , Perda do Osso Alveolar/cirurgia , Biodegradação Ambiental , Transplante Ósseo , Implantação Dentária Endóssea , Planejamento de Prótese Dentária , Prótese Dentária Fixada por Implante , Remoção de Dispositivo , Durapatita/metabolismo , Feminino , Humanos , Doenças Maxilares/cirurgia , Seio Maxilar/cirurgia , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Pré-Protéticos Bucais , Suporte de Carga
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