RESUMO
Reported are the results of a multicentre study involving 40 laboratories that was carried out in France to assess all the currently available methods used for the serodiagnosis of toxoplasmosis. For this purpose 10 batches of control sera were prepared with titres in the range 0-260 IU per ml. These sera were tested in nine laboratories using immunofluorescence methods; in three laboratories using dye tests; in forty laboratories using enzyme-linked immunosorbent assay; in four laboratories using direct agglutination and haemagglutination; in seven laboratories using the high-sensitivity IgG agglutination test; and in three laboratories using the latex agglutination test. In this way, 70 series of titrations were carried out using seven procedures and the results were compared with those obtained using the WHO reference serum in 15 cases, with the French national E6 serum in 16 other cases, and in 39 cases using 15 reference sera supplied by the reagent manufacturers. Rigorous comparison of the tests was not possible in all cases because one aim of the study was to ensure that the tests were carried out under the usual working conditions that prevailed in the participating laboratories. The results obtained indicate that the serological tests currently available for toxoplasmosis are acceptable for its serodiagnosis. Presentation of the titres in IU has advantages; however, caution is required since the definition of IU varies according to the test and reagents used. It is therefore essential that the conditions and limits for a positive reaction be carefully defined in each case, especially for commercially available kits.
Assuntos
Testes Sorológicos/métodos , Toxoplasmose/imunologia , Testes de Aglutinação , Ensaio de Imunoadsorção Enzimática , Imunofluorescência , Testes de Hemaglutinação , Humanos , Testes de Fixação do Látex , Padrões de Referência , Toxoplasmose/diagnósticoRESUMO
One hundred twenty-nine European laboratories participated in a collaborative, multicentre study designed to evaluate the overall reliability of different serological techniques for diagnosis of Toxoplasma gondii infection. Five freeze-dried reference sera were distributed to each laboratory, each of which analysed the sera with its routine methods. The enzyme-linked immunosorbent assay was the technique used most frequently, followed by the immunofluorescent antibody technique. Only nine laboratories performed the Sabin-Feldman dye test. In general, there was good concordance between qualitative results, but for sera with low concentrations of Toxoplasma gondii-specific IgG antibodies, some false-negative results were found. For specific IgM and IgA antibodies, the immunosorbent agglutination assay proved the most sensitive. The present study demonstrates the need for regular assessment of laboratory serodiagnosis of Toxoplasma gondii infection.
Assuntos
Anticorpos Antiprotozoários/sangue , Toxoplasma/imunologia , Animais , Imunofluorescência , Humanos , Técnicas Imunoenzimáticas , Imunoglobulina M/sangue , Testes SorológicosRESUMO
A study made among drug addicts in the Northern suburbs of Paris enables establishing a stabilisation of positive H.I.V. serology prevalence among them, a fact related to the free sale of syringes and needles. In Africa, the nosocomial transmission of H.I.V. by non-sterilisable but reused needles and syringes is demonstrated, and two cases are described. Its importance is certain but must be precise. The prevention of this epidemiological modality must be done by systematic use of reusable and sterilisable injectable material.
PIP: Reuse of disposable needles and syringes plays an important role in the transmission of HIV in France as in Africa. From September 1985-March 1986, 51% of drug addicts systematically screened in the northern suburbs of Paris were found to be HIV positive. Studies in the same population through 1988 indicated that the rate of HIV infection had remained stable, as had the average age of addicts, duration of addiction, and sex ratio. Stabilization of seropositivity rates among drug addicts in the northern suburbs in believed to have been due to information programs alerting addicts to the risk of contamination through sharing of needles and to the legal sale of syringes and needles instituted in May 1987. HIV transmission through reuse of disposable needles and syringes has been demonstrated in Africa. It is attributable to the poverty of health services and of the general population. The significance of HIV transmission by reuse of disposable materials in the health services appears to be considerable but is difficult to quantify. Prevention of transmission by this means will require correct use of sterilizable needles and syringes.