RESUMO
We describe colistin-resistant KPC-2-producing Klebsiella pneumoniae isolates from cerebrospinal fluid, belonging to ST423, selected during treatment for neuroinfection. Colistin resistance was related to mgrB gene interruption by an IS5-like.
Assuntos
Colistina/farmacologia , Farmacorresistência Bacteriana/genética , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae , beta-Lactamases/genética , DNA Bacteriano/líquido cefalorraquidiano , Humanos , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Testes de Sensibilidade Microbiana , Mutagênese Insercional/genéticaRESUMO
Here, we report the isolation of 31 Acinetobacter baumannii strains producing OXA-253 in a single large Brazilian city. These strains belonged to five different sequence types (STs), including 4 STs not previously associated with blaOXA-253 In all strains, the blaOXA-253 gene was located in a plasmid within a genetic environment similar to what was found previously in Brazil and Italy. The reported data emphasize the successful transmission of the blaOXA-253 gene through a large area and the tendency for this resistance determinant to remain in the A. baumannii population.
Assuntos
Acinetobacter baumannii/efeitos dos fármacos , Antibacterianos/farmacologia , beta-Lactamases/metabolismo , Acinetobacter baumannii/enzimologia , Acinetobacter baumannii/genética , Brasil , Hospitais , Itália , Testes de Sensibilidade Microbiana , Plasmídeos/genética , beta-Lactamases/genéticaRESUMO
The open process used to ferment sugar cane juice or molasses to produce ethanol fuel is prone to contamination by bacterial cells of different species, in particular Lactobacilli. The situation can be exacerbated by the emergence of resistant cells to industrial antibiotics that are normally used to combat this contamination. In this work, two Lactobacillus vini isolates from ethanol distilleries were identified and found to be resistant to doxycycline, a tetracycline derivative, although sensitive to other antibiotics tested. The identification of these isolates was confirmed by sequencing the pheS gene and their clonal origin was shown by PCR-fingerprinting analysis. Moreover, the isolates were shown to carry the transposable element Tn916 that harboured the tet-M gene. Furthermore, conjugation experiments showed that both isolates were capable of transferring this element, and as a result, the tet-M gene, to Enterococcus faecalis reference strain. Finally, the identification of tetracycline resistance in the same distilleries in other Lactobacilli, suggested that inter-species transfer of antibiotic resistance may be occurring in the industrial environment, and thus impairing the efficiency of the antibiotic treatment and causing serious health concerns.
Assuntos
Elementos de DNA Transponíveis , Microbiologia Industrial , Lactobacillus/efeitos dos fármacos , Lactobacillus/genética , Resistência a Tetraciclina , Antibacterianos/metabolismo , Conjugação Genética , Impressões Digitais de DNA , DNA Bacteriano/genética , Doxiciclina/metabolismo , Enterococcus faecalis/efeitos dos fármacos , Enterococcus faecalis/genética , Transferência Genética Horizontal , Genótipo , Lactobacillus/classificação , Reação em Cadeia da PolimeraseAssuntos
Antibacterianos/farmacologia , Carbapenêmicos/farmacologia , Enterobacter/efeitos dos fármacos , Enterobacter/enzimologia , Infecções por Enterobacteriaceae/microbiologia , Resistência beta-Lactâmica , beta-Lactamases/metabolismo , Brasil , Enterobacter/genética , Enterobacter/isolamento & purificação , Genótipo , Humanos , Testes de Sensibilidade Microbiana , beta-Lactamases/genéticaRESUMO
OBJECTIVES: Qnr-mediated quinolone resistance is increasingly detected worldwide, but few studies have been carried out so far in Brazil. The aim of this study was to test for qnr determinants in isolates of ciprofloxacin-resistant Klebsiella pneumoniae. METHODS: Fifteen ciprofloxacin-resistant isolates from urine cultures of hospitalized patients at a university hospital in North-East Brazil were investigated. Specific PCRs were performed for blaCTX-M and blaTEM, qnr, and class 1 integrons. Plasmid analyses and sequence type (ST) determination were performed, as described previously. RESULTS: The KP 930 isolate showed qnrA1 and blaTEM-1, together with dfrA12 and aadA2 in a class 1 integron. The qnr gene was located in a 133-kb plasmid. Multilocus sequence typing classified the isolate as ST147. CONCLUSIONS: We identified the combination of qnr with ST147 in Brazil; this is a clone that has disseminated widely and successfully in Latin America. The purpose of describing Qnr-mediated quinolone resistance in North-East Brazil is to draw attention to the spread of this mechanism in the country.
Assuntos
Antibacterianos/farmacologia , Ciprofloxacina/farmacologia , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Plasmídeos/genética , Adulto , Brasil , Resistência Microbiana a Medicamentos/genética , Feminino , Genes Bacterianos , Humanos , Integrons , Klebsiella pneumoniae/isolamento & purificaçãoRESUMO
Two new examples of OXA-72-producing Acinetobacter baumannii isolate resistant to a broad spectrum of antimicrobials, but not polymyxin B, have been identified in Recife, Brazil. Molecular typing indicated a close genetic link with the OXA-72-producing A. baumannii previously isolated in São Paulo, suggesting the possibility of clonal dissemination within the country.