Diphenyl diselenide protects cultured MCF-7 cells against tamoxifen-induced oxidative DNA damage.

Diphenyl diselenide (DPDS) is an electrophilic reagent used in the synthesis of a variety of pharmacologically active organoselenium compounds. Studies have shown its interesting pharmacodinamic properties, as antioxidant, antimutagenic and antitumoral effects. Here we report the antigenotoxic properties of DPDS against tamoxifen (TAM)-induced oxidative DNA damage in MCF-7 cultured cell line. We determined the cytotoxicity by lactate dehydrogenase (LDH) leakage assay and evaluated oxidative DNA damage by modified comet assay employing the enzymes formamidopyrimidine DNA-glycosylase (Fpg) and endonuclease III (Endo III). Our results demonstrate that the cellular effects of DPDS appear to be complex and concentration-dependent. The present findings show that DPDS is not genotoxic (at concentrations lower than 2.0µmol/L) in MCF-7 cells, as observed in the modified comet assay. Moreover, DPDS protects against TAM-induced oxidative DNA damage, probably by its antioxidant activity, without interfering with its cytotoxicity. In this manner, the treatment with low concentrations of DPDS, a synthetic organoselenium compound, could be used as a potent antigenotoxic agent to prevent the risk of cancer induction triggered by tamoxifen hormone therapy. Thereby, more studies concerning the toxicity of DPDS and its structural derivatives are still necessary for future safe therapeutic application and development of novel chemopreventive compounds for combined therapy in breast cancer.

Evaluation of the effects and mechanisms of action of flutriafol, a triazole fungicide, on striatal dopamine release by using in vivo microdialysis in freely moving rats.

The purpose of the present work was to assess the effects of flutriafol, a triazole fungicide, on in vivo dopamine (DA) release from rat striatum, using brain microdialysis coupled to high-performance liquid chromatography with electrochemical detection (HPLC-EC). Intrastriatal administration of flutriafol (1, 6 and 12 mM) produced significant concentration-dependent increases in DA levels to 218.5+/-51%, 1376+/-245% and 3093+/-345% compared with basal values, respectively. Those increases in DA levels could be due to an increased DA exocytotic release and/or a change in the activity of DA transporter (DAT). Thus, we investigated the effects of flutriafol (6mM) under Ca(++)- or Na(+)-free conditions, and after pretreatment with reserpine and TTX. When flutriafol was perfused in either Ca(++)- or Na(+)-free Ringer, the DA levels reduced 92% and 70%, respectively; perfusion of flutriafol in TTX-treated (10 microM) or reserpine-pretreated animals (10mg/kg), reduced the levels of DA to 73% and 86%, respectively. Co-infusion of flutriafol and nomifensine (20 microM) shows that the flutriafol-induced DA release did not involve the DAT. Our results suggest that flutriafol induces DA release via vesicular-, Ca(++)-, Na(+)- and TTX-dependent mechanism, being independent of DAT.

Relative effectiveness of iron bis-glycinate chelate (Ferrochel) and ferrous sulfate in the control of iron deficiency in pregnant women.

The relative effectiveness of daily supplementation of iron deficiency during pregnancy using 15 mg/day of iron from iron-bis-glycinate chelate (71 pregnant women), or 40 mg iron from ferrous sulfate (74 pregnant women) was evaluated by measuring hemoglobin, transferrin saturation and serum ferritin, at the beginning of the study (< 20 weeks of pregnancy) and at 20-30 weeks and 30-40 weeks thereafter. Ingestion for 13 weeks or more was considered adequate. Seventy three percent of the Ferrochel consuming group and 35% of the ferrous sulfate consuming group were considered to have taken the treatment adequately. The decrease in levels of all the measured parameters was significantly less pronounced in the group that consumed Ferrochel in spite of the lower treatment dose. Iron depletion was found in 30.8% of the women treated with Ferrochel and in 54.5% of the women than consumed ferrous sulfate. Of the factors responsible for non compliance taste was reported in 29.8% of the ferrous sulfate consumers and none in the groups that consumed Ferrochel. It is concluded that daily supplementation with Ferrochel was significantly more effective, in spite of the lower dose, than supplementation with ferrous sulfate.

[Changes in nutritional status of pregnant women assisted at the basic health network, Santo Andre, Brazil]. / Evolución del estado nutricional de embarazadas atendidas en la red basica de salud, Santo Andre, Brasil.

Nutritional conditions in the pre-gestacional period and during pregnancy were evaluated in 372 pregnant women based on the body mass index and Rosso's Graph. Approximately half (51%) the pregnant women presented normal pre-gestacional weight; however, 17.7% were underweight and 31.3% overweight. In the third trimester, 18.8% were underweight and 28.2% overweight. The weight at birth increased as nutritional conditions during pregnancy improved. These results show the importance of appropriate anthropometric control in order to prevent a bad neonatal prognosis and reaffirms the importance of such activity during the whole routine of pre-natal control.

[Rice and bean diets increase hepatic and plasmatic activity of gammaglutamyltranspeptidase in young rats]. / Dietas à base de arroz e feijão aumentam a atividade plasmática e hepática da gama-glutamiltranspeptidadase em ratos jovens.

The effect of feeding rice and bean diets in both hepatic and plasmatic activity of gamma-glutamyltranspeptidase-(GGT-EC 2.3.2.2) activity was evaluated in growing-rats (Experiment A) and mature rats (Experiment B). During 28 days, the animals were fed with isocaloric-diets composed by tree levels of rice, bean or rice-and-bean protein. Similarly with the aproteic group, a significant increase on both the hepatic and plasmatic GGT activity were showed with the lowest levels of protein, when compared with 25% casein control group. This rise was more effective in growing-rats fed on legume-based diets (as bean or rice-and-bean diets), making evident a differential effect of age and an exacerbated effect of the protein restriction with the lowest sulfur amino acids disposal. These alterations suggest a metabolic adaptation of GGT to both the inadequate protein and sulfur-amino acid levels, thus supporting the hypothesis that the Glutathione levels may be reduced by these legume-based diets.

[Iron nutritional status in pregnant adolescents, São Paulo, Brazil]. / Estado nutricional del hierro de gestantes adolescentes, São Paulo, Brasil.

The frequency of anemia, iron deficiency and iron body stores was assessed in 155 pregnant teenagers of low socioeconomic status in a prenatal care unit of a beneficent hospital in São Paulo, Brazil. By the criterion of the World Health Organization (Hb < 12 g/dL) 14.2% of the pregnant adolescents had anemia. The iron deficiency diagnostic by saturation of transferrin < 16% and zinc protoporphyrin concentration > 60 (mol/mol heme were 45.8 and 42.6%, respectively. The iron body store (serum ferritin < 12 micrograms/L) was depleted for 48.4% of adolescents. It is concluded that the iron nutritional status of these adolescents were characteristics of the pregravidic inadequate iron store. Despite low percentage of the anemia, the high frequency of iron deficiency and depleted iron stores suggest a practical procedure to detect iron deficiency and the use of iron supplementation in teenagers.

DL-methionine supplementation of rice-and-bean diets affects gamma-glutamyltranspeptidase activity and glutathione content in livers of growing rats.

Gamma-glutamyltranspeptidase (GGT-EC 2.3.2.2) activity and glutathione (GSH) content were measured in livers of female weanling Wistar rats (N = 5-18), submitted to rice-and-bean diets (13 and 6% w/w protein), both supplemented or not with DL-methionine (0.5 and 0.23 g/100 g dry diet, respectively). After 28 days, the rats on the rice-and-bean diets showed significantly higher levels (four times higher) of liver GGT activity and a concomitant 50% lower concentration of liver GSH in comparison with control groups feeding on casein. The addition of DL-methionine to rice-and-bean diets significantly increased the liver GSH content, which reached levels 50% higher than those found in animals on casein diets. The increase in GSH was accompanied by a decrease in liver GGT activity, which did not reach levels as low as those observed in the control groups. No significant correlation could be established between GGT and GSH changes under the present experimental conditions. Linear correlation analysis only revealed that in animals submitted to unsupplemented rice-and-bean diets GSH concentration was positively associated (P < 0.05) with weight gain, food intake and food efficiency. GGT, however, was negatively correlated (P < 0.05) with food intake only, and exclusively for supplemented rice-and-bean diets. The high levels of GGT activity observed in the present study for rats receiving a rice-and-bean mixture could be a result of the poor quality of these diets associated with their deficiency in sulfur amino acids. The results also suggest that diet supplementation with methionine could be important in the reduction of the deleterious effects of GSH depletion by restoring the intracellular concentration of this tripeptide.

DL-methionine supplementation of rice-and-bean diets affects gamma-glutamyltranspeptidase activity and glutathione content in livers of growing rats

Gamma-glutamyltranspeptidase (GGT-EC 2.3.2.2) activity and glutathione (GSH) content were measured in livers of female weanling Wistar rats (N = 5-18), submitted to rice-and-bean diets (13 and 6 per cent w/w protein), both supplemented or not with DL-methionine (0.5 and 0.23 g/100 g dry diet, respectively). After 28 days, the rats on the rice-and-bean diets showed significantly higher levels (four times higher) of liver GGT activity and a concomitant 50 per cent lower concentration of liver GSH in comparison with control groups feeding on casein. The addition of DL-methionine to rice-and-bean diets significantly increased the liver GSH content, which reached levels 50 per cent higher than those found in animals on casein diets. The increase in GSH was accompanied by a decrease in liver GGT activity, which did not reach levels as low as those observed in the control groups. No significant correlation could be established between GGT and GSH changes under the present experimental conditions. Linear correlation analysis only revealed that in animals submitted to unsupplemented rice-and-bean diets GSH concentration was positively associated (P<0.05) with weight gain, food intake and food efficiency. GGT, however, was negatively correlated (P<0.05) with food intake only, and exclusively for supplemented rice-and-bean diets. The high levels of GGT activity observed in the present study for rats receiving a rice-and-bean mixture could be a result of the poor quality of these diets associated with their deficiency in sulfur amino acids. The results also suggest that diet supplementation with methionine could be important in the reduction of the deleterious effects of GSH depletion by restoring the intracellular concentration of this tripeptide.

[Nutritional status of adolescent pregnancy in São Paulo, Brazil]. / Estado nutricional de gestantes adolescentes en São Paulo, Brasil.

Several anthropometric patterns were used to evaluate the nutritional status of 155 pregnant teenagers of low socioeconomic status in a prenatal care unit of a beneficent hospital in São Paulo, Brazil. Using the body mass index 25.2% of them were classified as underweight before pregnancy and 3.3% during pregnancy. The Siqueira's et al curve and the Rosso's normogram showed higher percentages of underweight, as well as of overweight and obesity. The results indicated the necessity to review the anthropometrics patterns that classify specifically the nutritional status of pregnant adolescents.

[Prevalence of iron deficiency anemia in female adolescents in Taboaoa da Serro, SP. Brazil]. / Prevalência de anemia e deficiência de ferro em adolescentes do sexo feminino-Taboão da Serra, SP, Brasil.

It was studied an equiprobabilistic sample of 262 female teenagers living in the city of Taboão da Serra, Brazil, with the aim of evaluating the prevalence of anemia and iron-deficiency. Anemia was identified by the hemoglobin level and the prevalence of iron-deficiency was determined through the concentration of free erythrocyte protoporphyrin. It was observed a prevalence of 17.6% of anemia, and its occurrence was inversely related to factors indicative of the economic-social level: income, schooling of parents and home's characteristics. The presence of anemia was higher in the group that did not have reached menarche, and its occurrence did not show relation with the interval between menstrual cycle. The prevalence of iron-deficiency was of 29,4% and it was not verified correlation between the bloody hemoglobin and the free erythrocyte protoporphyrin, except in severe cases of anemia.

Differential effects of protein restriction on gamma-glutamyltranspeptidase [EC 2.3.2.2] activity in young and mature rats.

Gamma-glutamyltranspeptidase (GGT, EC 2.3.2.2) activity was determined in the plasma and liver of 40 young (50 days old) and 40 mature (300 days old) male Wistar rats, after a protein restriction period of 28 days. Casein protein levels used were: 1%, 3%, 5%, 7% and 28% (control). Weanling rats submitted to protein-free diet and adult rats submitted to that and other low-protein diets (1%, 3% or 5% casein) presented weight reductions (-0.38 +/- 0.07 g/day and -0.98 +/- 0.19 g/day, respectively). Only in young animals did these weight reductions parallel those of food consumption (37 +/- 12% of control), plasma protein (52 +/- 11% of control), plasma albumin (70 +/- 12% of control), hepatic RNA (68 +/- 7% of control) and protein (71 +/- 8% of control). A marked effect of the protein restriction, increasing the GGT activity, was also observed only in young rats. A significant (P < 0.05) rise was promoted by the protein-free diet in the plasma GGT (2.83 +/- 1.39 vs 0.69 +/- 0.50 mU/ml for control) and by both the protein-free and 3% casein diets, in the liver GGT (respectively, 16.00 +/- 6.72 and 7.75 +/- 3.49 vs 0.94 +/- 0.57 U/g protein for control). The different results obtained for young in relation to mature animals could be explained by the reduction of both protein and sulfur-containing amino acid requirements with aging.

[Nutritional and disease diagnosis in institutionalized children. Day care center Elisio Teixeira Leite, Perus, SP 1993]. / Diagnóstico nutricional e de saúde de crianças institucionalizadas - creche Elísio Teixeira Leite, Perus, SP, 1993.

As a part of one survey about health conditions in institutionalized children, the nutritional status were evaluated. Data were obtained through antropometric assessment in a sample of 111 pre-school children matriculated in a small official day-care center in Perus-SP. According to Gomez, 50.0% of all that children were classified as malnourished, 23.4% of them in the I degree; 22.4% in II degree and only 4.3% in III degree. Taking into account the Waterlow's classification there was a highest frequency of cronic-actual malnutrition (30.8%) concentrated in children between 12-48 months of age. Additionally, these children presented highest incidence of infective-respiratory and intestinal diseases. These evidences lead on to the improvement of the follow-up of children growth and food consumption, specially that under 24 meses, to attempt for an early identification of malnutrition, thus increasing the chance of nutritional recuperation.

[Nutrition evaluation of female university students]. / Avaliação nutricional de estudantes universitários do sexo feminino.

The self-evaluation of nutritional status, utilized as a teaching strategy of introductory nutrition course at Nursing School (São Paulo's University), gave a subsidy to this work. With this purpose, 50 female university students 3rd'semester (mean age was 21.7) were required to complete a three days food record and report their approximate height and weight. The analysis of food intake adequacy indicated that 98% of the students had insufficient intake (less than 90% of recommended of National Research Council-1980) of, at least, one of the nutrients (protein, calcium, iron, vitamin A, B1, B2, C and niacin). It was evidenced an inadequate intake (less than 90% of RDA) of calcium (80% of the students), vitamin A (72%), iron (64%), vitamin B1 (74%), vitamin B2 (56%) and vitamin C (34%). The inadequate intake was more frequently and serious (less than 60% RDA) for calcium (42%), vitamin A (20%) and iron (16%) showing a vitamins and minerals deficient diet.