In vivo antifungal activity and biocompatibility of Cryptocarya moschata.

The objective of this study was evaluate, in vivo model, the antifungal activity of Cryptocarya moschata extract against Candida albicans and its biocompatibility. The animals (N = 50) were divided into groups (n = 5): CI/CG: candidiasis was induced and treated with C. moschata extract (0.045 g/mL); CI/NG: candidiasis was induced and treated with nystatin; CI/NT: candidiasis was induced and no treated; CI/CG-2: candidiasis was induced and treated with C. moschata extract (0.045 g/mL), reapplied after 24 h; CI/NG-2: candidiasis was induced and treated with nystatin, reapplied after 24 h; NCI/NT: candidiasis was not induced and no treated; NCI/CG: candidiasis was not induced and treated with C. moschata extract (0.045 g/mL); NCI/NG: candidiasis was not induced treated with nystatin; NCI/CG-2: candidiasis was not induced and treated with C. moschata extract (0.045 g/mL), reapplied after 24 h; NCI/NG-2: candidiasis was not induced and treated with nystatin, reapplied after 24 h. The fungi present in the lingual dorsum of mice were collected and analyzed by the count of colony-forming units. In addition, histological analysis was performed. Histologically, there was no cell damage in the mice's tongue, and there was a decrease in Candida biofilm, similar to the use of nystatin. It was concluded that the C. moschata extract was effective against C. albicans and was biocompatible.

Effectiveness of Disinfectant Liquid Soaps in the Reduction of Candida spp Present in Complete Dentures: A Crossover Randomized Clinical Trial.

PURPOSE: To evaluate the effectiveness of liquid disinfectant soaps for the reduction of microorganisms present on maxillary complete dentures. MATERIALS AND METHODS: The selected patients (N = 28) were randomly divided into four groups (n = 7), and each group underwent all four disinfection treatments in a different order. The disinfection treatments evaluated were: 0.5% sodium hypochlorite (positive control); Dettol liquid soap; Lifebuoy liquid soap; and phosphate-buffered saline solution (negative control). The patients were instructed to immerse their maxillary dentures in the disinfectant solution for 8 hours (overnight) for 7 days, with the solutions in a randomized sequence with a washout period of 1 week between solutions. Biofilm samples of the dental prostheses were obtained before and after each treatment with a sterile swab, and the microbiologic material was diluted and plated in selective media for Candida spp. Colony-forming unit count (CFU/mL) was performed in each group. One-way ANOVA with Welch correction was used for analysis, with Games-Howell post hoc test with a significance level of .05. RESULTS: A 3-log reduction in microorganisms was considered effective compared to baseline. The highest incidence observed was for Candida albicans, which presented with a frequency between 66% and 92%, followed by C tropicalis, with a frequency between 7% and 33%, in all experimental groups. Sodium hypochlorite was able to reduce more than 3 log10 of microorganisms for all patients, showing high antifungal effectiveness for both C albicans and C tropicalis species. Regarding the experimental groups, both liquid soaps (Dettol and Lifebuoy) were effective in reducing the two types of microorganisms. CONCLUSION: Liquid soaps were effective at reducing biofilm and may be an alternative for disinfection of removable partial dentures or complete dentures.