RESUMO
The microbiome research field is rapidly evolving, but the required biobanking infrastructure is currently fragmented and not prepared for the biobanking of microbiomes. The rapid advancement of technologies requires an urgent assessment of how biobanks can underpin research by preserving microbiome samples and their functional potential.
Assuntos
Bancos de Espécimes Biológicos/normas , Microbiota , Animais , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Bancos de Espécimes Biológicos/tendências , Pesquisa Biomédica , Humanos , Mamíferos/microbiologia , Plantas/microbiologia , Preservação BiológicaRESUMO
This paper describes a new method for recognizing hand configurations of the Brazilian Gesture Language - LIBRAS - using depth maps obtained with a Kinect® camera. The proposed method comprised three phases: hand segmentation, feature extraction, and classification. The segmentation phase is independent from the background and depends only on pixel depth information. Using geometric operations and numerical normalization, the feature extraction process was done independent from rotation and translation. The features are extracted employing two techniques: (2D)2LDA and (2D)2PCA. The classification is made with a novelty classifier. A robust database was constructed for classifier evaluation, with 12,200 images of LIBRAS and 200 gestures of each hand configuration. The best accuracy obtained was 95.41%, which was greater than previous values obtained in the literature.
Assuntos
Processamento de Imagem Assistida por Computador/métodos , Língua de Sinais , Adolescente , Adulto , Brasil , Bases de Dados Factuais , Feminino , Gestos , Mãos , Humanos , Idioma , Masculino , Adulto JovemRESUMO
OBJECTIVE: To determine the influence of prenatal zidovudine (ZDV) prophylaxis on the course of HIV- 1 infection in children by comparing the clinical outcome of infants born to HIV- 1-seropositive mothers who did versus those who did not receive ZDV during pregnancy. METHODS: Medical records of HIV-1-seropositive mothers and their infants were reviewed retrospectively. Participants were divided according to maternal ZDV use: no ZDV (n = 152); ZDV (n = 139). The main outcome measure was rapid disease progression (RPD) in the infant, defined as occurrence of a category C disease or AIDS-related death before 18 months of age. RESULTS: HIV vertical transmission rates were significantly different (no ZDV versus ZDV: 22.3% versus 12.2%; p = .034). Among infected infants, the RPD rate was 29.4% in the no ZDV group compared with 70.6% in the ZDV group (p = .012), and prematurity was significantly associated with a higher risk of RPD (p = .027). CONCLUSIONS: The rate of RPD was significantly higher among perinatally infected infants born to HIV-infected mothers treated with ZDV than among infected infants born to untreated mothers. The decreased proportion of infected infants with nonrapid disease progression in the former group might be related to the ability of ZDV to block intrapartum transmission preferentially and also to nonrapid disease progression resulting from intrapartum transmission.
Assuntos
Fármacos Anti-HIV/uso terapêutico , Infecções por HIV/tratamento farmacológico , Infecções por HIV/transmissão , Soropositividade para HIV/tratamento farmacológico , HIV-1 , Transmissão Vertical de Doenças Infecciosas , Complicações Infecciosas na Gravidez/virologia , Efeitos Tardios da Exposição Pré-Natal , Zidovudina/uso terapêutico , Contagem de Linfócito CD4 , Progressão da Doença , Feminino , Idade Gestacional , Infecções por HIV/mortalidade , Humanos , Lactente , Recém-Nascido , Recém-Nascido Prematuro , Transmissão Vertical de Doenças Infecciosas/estatística & dados numéricos , Gravidez , Complicações Infecciosas na Gravidez/tratamento farmacológico , Estudos Retrospectivos , Fatores de RiscoRESUMO
Many factors can lead cells to apoptosis during the various stages of cell life. This study was undertaken to characterize germ cell death in the epididymis of the adult Artibeus lituratus by histochemical and immunohistochemical techniques using light microscopy and transmission electron microscopy. The results showed that cells with a nuclear phenotype and ultrastructural characteristics of chromatin compaction were common in apoptosis. The Apoptag test confirmed that the suspected cells were apoptotic. It is suggested that immature germ cells, when released from the germinative epithelium, may be directed towards the epididymis instead of being disposed of in the testicle. Furthermore, intact immature cells can leave the testicle in the initial phases of apoptosis and complete this phenomenon in the epididymis.
Assuntos
Apoptose , Epididimo/patologia , Espermatozoides/patologia , Animais , Quirópteros , Masculino , Microscopia Eletrônica , Espermatozoides/ultraestruturaRESUMO
Oral myofibroma is an uncommon, benign, solitary proliferation of myofibroblastic tissue. Few cases affecting maxillofacial region have been reported. We present a case of gingival myofibroma, diagnosed on clinical, histopathological, immunohistochemical, and ultrastructural basis.
