RESUMO
The Extensible Markup Language (XML) is a newly adopted Internet protocol for data interchange designed to bring the key features of the Standard Generalized Markup Language (SGML; ISO 8879:1986)--extensibility, complex structures, and validation--to the World Wide Web. In this paper, we describe an architecture that uses XML to mediate between disparate client-server systems for structured reporting and decision support.
Assuntos
Técnicas de Apoio para a Decisão , Sistemas Computadorizados de Registros Médicos/organização & administração , Linguagens de Programação , Integração de Sistemas , Teorema de Bayes , Sistemas de Apoio a Decisões Clínicas , Humanos , Internet/normas , Sistemas Computadorizados de Registros Médicos/normas , SoftwareRESUMO
Tn5 is a composite transposon consisting of two IS50 sequences in inverted orientation with respect to a unique, central region encoding several antibiotic resistances. The IS50R element encodes two proteins in the same reading frame which regulate the transposition reaction: the transposase (Tnp), which is required for transposition, and an inhibitor of transposition (Inh). The inhibitor is a naturally occurring deletion variant of Tnp which lacks the N-terminal 55 amino acids. In this report, we present the purification of both the Tnp and Inh proteins and an analysis of their DNA binding properties. Purified Tnp, but not Inh, was found to bind specifically to the outside end of Tn5. Inh, however, stimulated the binding activity of Tnp to outside-end DNA and was shown to be present with Tnp in these bound complexes. Inh was also found to exist as a dimer in solution. These results indicate that the N-terminal 55 amino acids of Tnp are required for sequence-specific binding. They also suggest that Inh inhibits transposition by forming mixed oligomers with Tnp which still bind to the ends of the transposon but are defective for later stages of the transposition reaction.