RESUMO
Results of 106 in vitro fertilization procedures were used to evaluate the usefulness of tests of human sperm function for predicting fertilization rates. Sperm tests included concentration, motility, morphology, vitality (eosin Y exclusion), nuclear immaturity (aniline blue stain), and hypo-osmotic swelling. Only the number of sperm in the insemination medium, percentage normal morphology, and vitality were statistically significant in logistic regression models of fertilization rates. The other tests, such as the hypo-osmotic swelling test, did not give additional information about fertilization rates in this study. It is concluded that logistic regression analysis of factors affecting results of fertilization in vitro provides a powerful tool for evaluating some clinical tests of sperm function.
Assuntos
Fertilização in vitro , Sêmen/análise , Espermatozoides/fisiologia , Feminino , Humanos , Infertilidade/terapia , Masculino , Análise de RegressãoAssuntos
Clomifeno/uso terapêutico , Fertilização in vitro/efeitos dos fármacos , Menotropinas/uso terapêutico , Menstruação/efeitos dos fármacos , Ensaios Clínicos como Assunto , Transferência Embrionária , Estradiol/sangue , Feminino , Humanos , Indução da Ovulação , Gravidez , Estudos ProspectivosRESUMO
The effects of maternal preovulatory serum and human fetal cord serum supplement in culture medium in human in vitro fertilization and embryo development were compared in 208 cases over an 8-month period when there were no significant changes in other variables. A significantly higher pregnancy rate was observed in the fetal cord serum group despite no significant difference being found in fertilization and embryo cleavage rates. This suggests a difference in the health of the embryo cultured in different serum supplements.
