1.
Comb Chem High Throughput Screen
; 6(4): 313-20, 2003 Jun.
Artigo
em Inglês
| MEDLINE
| ID: mdl-12769674
RESUMO
Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format. Here, we illustrate the application of fluorescence read-out technologies for kinase targets in light of our own experiences in assay development and high-throughput screening.
Assuntos
Fluorometria/métodos , Proteínas Quinases/análise , Animais , Técnicas de Química Combinatória/métodos , Corantes Fluorescentes/química , Corantes Fluorescentes/metabolismo , Humanos , Inibidores de Proteínas Quinases , Proteínas Quinases/metabolismo
2.
Chembiochem
; 3(10): 1023-6, 2002 Oct 04.
Artigo
em Inglês
| MEDLINE
| ID: mdl-12362369