RESUMO
The possible association between susceptibility to infection with Cryptococcus neoformans var. gattii and HLA phenotype was examined in a group of Papua New Guinean patients. There was no evidence for a statistically significant association between susceptibility to infection and HLA class I and HLA class II phenotypes, although analysis of data which had not been subjected to the appropriate Bonferroni correction factor suggested a trend for susceptibility linked to HLA B*5601.
Assuntos
Criptococose/genética , Criptococose/imunologia , Predisposição Genética para Doença/imunologia , Antígenos HLA-D/imunologia , Antígenos de Histocompatibilidade Classe I/genética , Complexo Principal de Histocompatibilidade , Cryptococcus neoformans , Predisposição Genética para Doença/microbiologia , Humanos , Papua Nova Guiné , FenótipoRESUMO
We have developed a simple, rapid and reliable method for specifically amplifying and cloning full-length HLA-B genes from genomic DNA. Using this methodology we characterized three alleles of interest at the molecular level. Two of the alleles appeared in our routine class I PCR-SSOP typing system, a variant of B*5801 found in the Daudi cell line and RCE 56 and a variant of B*4101 found in a number of volunteer donors on our Bone Marrow Donor Registry. The third, a variant B35 allele found in RCE 80, was first identified as unusual by serology. Our sequencing analysis of exon 2 and exon 3 identified two of these alleles as the recently reported novel HLA-B*5802 and HLA-B*4102 alleles, while the third represents a new B35 allele officially designated B*3513.
Assuntos
Antígenos HLA-B/genética , Reação em Cadeia da Polimerase/métodos , Alelos , Sequência de Bases , DNA , Variação Genética , Antígeno HLA-B35/genética , Humanos , Dados de Sequência Molecular , Homologia de Sequência do Ácido Nucleico , Células Tumorais CultivadasRESUMO
The flow cytometric crossmatch is a technique that is increasingly being used by clinical transplant laboratories. In this multicenter study by the British Society for Histocompatibility and Immunogenetics Flow Cytometry Group, a series of crossmatches were carried out to determine whether different centers obtained same results when performing the same crossmatch. There was greater than 80% agreement among participating laboratories on the results of 35/54 tests. There was no clear agreement in the remaining 20 cases. Quantitative analysis, estimating the number of cell-bound fluorescein molecules, demonstrated that differences in the criteria used by each center to define a positive crossmatch were responsible for some discordant results. When applied, definition of positivity based on the molecules of fluorescein increased concordance from 57.5% to 81.4%.l. These results suggest that a criterion for the interpretation of results based on quantitative analysis of bound antibody may be more reliable than methods in current routine use.
