RESUMO
In patients with IgG incomplete non-complement binding warm autoantibodies, the subclass composition of the antibodies was studied in relation to the occurrence of increased haemolysis in vivo and the adherence of the patients red cells to peripheral blood monocytes (PBM) in vitro. The presence of IgG3 autoantibodies was almost always accompanied by haemolytic anaemia, but the presence of IgG1 autoantibodies only in some patients but not in others. IgG2 and IgG4 autoantibodies were not associated with increased red cell destruction. A relation identical to that between subclass composition and increased haemolysis was found between subclass composition and adherence of the patients erythrocytes to PBM and thus a strong correlation between positive adherence in vitro and increased red cell destruction in vivo. These results support an important role of adherence to mononuclear phagocytic cells in the destruction of red cells sensitized with non-complement binding IgG antibodies. Strong indications were found that IgG1 autoantibodies are of two kinds, only one of which causes adherence to phagocytes and thus increased red cell destruction.
Assuntos
Citotoxicidade Celular Dependente de Anticorpos , Hemólise , Imunoglobulina G , Monócitos , Fagócitos/imunologia , Anemia Hemolítica/imunologia , Autoanticorpos , Adesão Celular/efeitos dos fármacos , Eritrócitos/imunologia , Hemólise/efeitos dos fármacos , Humanos , Técnicas In Vitro , Prednisona/farmacologiaRESUMO
Highly purified C-type particles from milk of NZB mice were ineffective in the transfer of Coombs positive hemolytic anemia and cryoglobulinemia to neonatal BALB/cHeA and NZC/B1 mice. Injection of neonatal NZB mice with these particles did not change the frequency or time of onset of these phenomena. Antinuclear antibodies were found in all injected animals in the same frequencies as in the control groups.
Assuntos
Anemia Hemolítica Autoimune/etiologia , Doenças Autoimunes/etiologia , Transtornos das Proteínas Sanguíneas/etiologia , Crioglobulinas , Retroviridae , Animais , Animais Recém-Nascidos , Anticorpos Antinucleares/análise , Teste de Coombs , Feminino , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos NZB , Camundongos Endogâmicos , Leite/microbiologia , Ratos , Ratos Endogâmicos , Retroviridae/isolamento & purificação , Especificidade da Espécie , Timo/microbiologiaAssuntos
Transfusão de Sangue , Leucócitos/imunologia , Animais , Anticorpos , Antígenos/análise , Incompatibilidade de Grupos Sanguíneos/prevenção & controle , Preservação de Sangue , Separação Celular , Filtração , Congelamento , Gossypium , Humanos , Imunização , Controle de Qualidade , Coelhos , Diálise Renal , Extratos de Tecidos/administração & dosagemAssuntos
Transfusão de Sangue , Antígenos de Histocompatibilidade , Absorção , Aglutininas , Animais , Formação de Anticorpos , Especificidade de Anticorpos , Plaquetas/imunologia , Células da Medula Óssea , Transplante de Medula Óssea , Separação Celular/métodos , Sobrevivência Celular , Teste de Coombs , Eritrócitos/imunologia , Filtração , Imunofluorescência , Granulócitos/imunologia , Humanos , Imunização , Isoanticorpos/análise , Leucócitos/imunologia , Masculino , Coelhos , Imunologia de Transplantes , Transplante HomólogoRESUMO
The serum of 100 patients with myasthenia gravis and 441 of their first-degree relatives was studied for the presence of autoantibodies against several antigens. Antibodies to skeletal muscle were present in 22% of the patients and in 2% of the relatives. Both these frequencies were significantly higher than those in matched control subjects. Also, antinuclear antibodies were present more often both in the patients and in the relatives. Typing for HL-A antigens had shown a positive correlation between HL-A 8 and myasthenia gravis which was significantly higher in women than in men. Antibodies to skeletal muscle and thymomas were found to be much rarer in HL-A 8-positive patients than in HL-A 8-negative patients; HL-A 8-positive patients acquired the disease at an earlier age.HL-A 2-positive patients more often had thymomas and antibodies to skeletal muscle than HL-A 2-negative patients; HL-A 2-positive patients acquired myasthenia gravis at a later age.The fact that the clinical aspects of the HL-A 8-negative and HL-A 2-positive patients were different from those of the HL-A 8-positive and HL-A 2-negative patients justifies the hypothesis that there are two forms of myasthenia gravis.
