RESUMO
Several pathologies of the gastrointestinal tract, particularly food allergy, are due to an exaggerated and imbalanced response of the gut mucosal immune system. The intestinal microflora is an important constituent of the gut mucosal barrier against food allergens and there is increasing evidence that one important acquired factor predisposing to food allergy in infants is the gut microflora. Indeed, the balance of bifidobacteria versus Clostridia in the neonatal flora appears to determine the allergic status in infants. In earlier studies, it was shown that the higher prevalence of allergies in infants fed standard formulas, compared to breast-fed infants, correlated with lower frequencies of bifidobacteria in their faeces. Certain Lactobacillus probiotic strains can have an inhibitory impact on allergic inflammation. The mechanisms implicated are still unclear, but it seems that they can involve both proteolytic and/or immunomodulatory functions. One challenge will be to find a probiotic strain that elicits all these functions and that fulfills all safety criteria.
Assuntos
Hipersensibilidade Alimentar/terapia , Probióticos/uso terapêutico , Hipersensibilidade Alimentar/imunologia , Humanos , Lactente , Recém-Nascido , Intestinos/microbiologia , LactobacillusRESUMO
BACKGROUND: Mechanisms of systemic IgE suppression by oral tolerance have been extensively studied, but less is known about oral tolerance induction in mice challenged at mucosal sites. We have previously shown in systemically challenged mice that high-dose tolerance suppressed specific but not bystander IgE. In an attempt to mimic oral tolerance in food-allergic patients, we have investigated how IgE suppression could be induced in mice sensitized orally against beta-lactoglobulin (BLG). METHODS: Mice were immunized orally against BLG using cholera toxin as adjuvant. Before oral sensitization, mice were administered milk whey proteins, either in the form of a single high-dose gavage, or by prolonged ad libitum administration of various doses. RESULTS: Orally sensitized mice mounted a BLG-specific IgE response. In contrast to systemically challenged mice, a single high-dose gavage of whey protein given prior to the onset of oral sensitization resulted in the suppression of both specific and bystander IgE. When mice were fed moderate to low doses of milk whey proteins daily ad libitum in the drinking water during 3 weeks prior to oral sensitization, all doses effectively suppressed antigen-specific IgE. However, bystander IgE suppression was observed only at the lowest doses. When mice were tolerized during 4 days instead of 3 weeks, IgE titers remained unchanged. CONCLUSIONS: In orally sensitized mice, bystander IgE suppression depended on the dose of tolerogen, but also on its mode of administration. Mucosally induced IgE responses were suppressed by a mechanism that was distinct from that operating in the periphery.
Assuntos
Imunoglobulina E/biossíntese , Lactoglobulinas/imunologia , Administração Oral , Animais , Linfócitos T CD4-Positivos/metabolismo , Toxina da Cólera/farmacologia , Citocinas/biossíntese , Feminino , Imunoglobulina A/sangue , Imunoglobulina E/sangue , Imunoglobulina G/sangue , Imunoglobulina G/classificação , Camundongos , Camundongos Endogâmicos BALB CRESUMO
We investigated whether certain strains of lactic acid bacteria (LAB) could antagonize specific T-helper functions in vitro and thus have the potential to prevent inflammatory intestinal immunopathologies. All strains tested induced various levels of both interleukin-12 (IL-12) and IL-10 in murine splenocytes. In particular, Lactobacillus paracasei (strain NCC2461) induced the highest levels of these cytokines. Since IL-12 and IL-10 have the potential to induce and suppress Th1 functions, respectively, we addressed the impact of this bacterium on the outcome of CD4(+) T-cell differentiation. For this purpose, bacteria were added to mixed lymphocyte cultures where CD4(+) T-cells from naive BALB/c mice were stimulated weekly in the presence of irradiated allogeneic splenocytes. In these cultures, L. paracasei NCC2461 strongly inhibited the proliferative activity of CD4(+) T cells in a dose-dependent fashion. This was accompanied by a marked decrease of both Th1 and Th2 effector cytokines, including gamma interferon, IL-4, and IL-5. In contrast, IL-10 was maintained and transforming growth factor beta (TGF-beta) was markedly induced in a dose-dependent manner. The bacteria were not cytotoxic, because cell viability was not affected after two rounds of stimulation. Thus, unidentified bacterial components from L. paracasei NCC2461 induced the development of a population of CD4(+) T cells with low proliferative capacity that produced TGF-beta and IL-10, reminiscent of previously described subsets of regulatory cells implicated in oral tolerance and gut homeostasis.
Assuntos
Linfócitos T CD4-Positivos/imunologia , Interleucina-10/biossíntese , Lactobacillus/imunologia , Fator de Crescimento Transformador beta/biossíntese , Animais , Linfócitos T CD4-Positivos/citologia , Linfócitos T CD4-Positivos/microbiologia , Divisão Celular , Células Cultivadas , Técnicas de Cocultura , Feminino , Interferon gama/metabolismo , Interleucina-12/biossíntese , Interleucina-4/metabolismo , Interleucina-5/metabolismo , Ácido Láctico , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Baço/citologia , Baço/imunologia , Células Th1/imunologia , Células Th2/imunologiaRESUMO
One mechanism regulating the ability of different subsets of T helper (Th) cells to respond to cytokines is the differential expression of cytokine receptors. For example, Th2 cells express both chains of the interferon gamma receptor (IFN-gammaR), whereas Th1 cells do not express the second chain of the IFN-gammaR (IFN-gammaR2) and are therefore unresponsive to IFN-gamma. To determine whether the regulation of IFN-gammaR2 expression, and therefore IFN-gamma responsiveness, is important for the differentiation of naive CD4(+) T cells into Th1 cells or for Th1 effector function, we generated mice in which transgenic (TG) expression of IFN-gammaR2 is controlled by the CD2 promoter and enhancer. CD4(+) T cells from IFN-gammaR2 TG mice exhibit impaired Th1 polarization potential in vitro. TG mice also display several defects in Th1-dependent immunity in vivo, including attenuated delayed-type hypersensitivity responses and decreased antigen-specific IFN-gamma production. In addition, TG mice mount impaired Th1 responses against Leishmania major, as manifested by increased parasitemia and more severe lesions than their wild-type littermates. Together, these data suggest that the sustained expression of IFN-gammaR2 inhibits Th1 differentiation and function. Therefore, the acquisition of an IFN-gamma-unresponsive phenotype in Th1 cells plays a crucial role in the development and function of these cells.
Assuntos
Interferon gama/imunologia , Transdução de Sinais/imunologia , Células Th1/imunologia , Animais , Antígenos/imunologia , Divisão Celular , Polaridade Celular , Células Cultivadas , Feminino , Expressão Gênica , Hemocianinas/imunologia , Humanos , Memória Imunológica/imunologia , Listeria monocytogenes/imunologia , Listeriose/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Camundongos Transgênicos , Receptores de Interferon/genética , Receptores de Interferon/imunologia , Células Th1/citologia , Receptor de Interferon gamaRESUMO
A protective immune response to the intracellular parasite Leishmania major requires the development of a Th1 CD4+ T cell phenotype. We demonstrate herein that BALB/c mice, which normally develop a susceptible Th2 response to L. major infection, are protected when co-injected with an agonistic anti-murine CD40 mAb. Anti-CD40 mAb-mediated protection in this system was found to be T cell dependent, since it was not observed in C57BL/6 x 129 mice that were rendered T cell deficient (TCR beta-/- x TCR delta-/-) and L. major susceptible. Anti-CD40 mAb stimulation of L. major-infected BALB/c mice was accompanied by increased IL-12 and IFN-gamma production in draining lymph nodes, analyzed either by direct expression, or in an antigen-specific in vitro recall assay. The protective role of these cytokines was indicated by the finding that anti-CD40 mAb-mediated protection of L. major-infected BALB/c mice could be reversed by co-treating the animals with neutralizing anti-IL-12 and/or anti-IFN-gamma mAb. Collectively, these data suggest that BALB/c mice develop a protective Th1 CD4+ T cell response to L. major infection when co-injected with anti-CD40 mAb. While the CD40-CD40L interaction has been previously shown to be vital in the control of murine Leishmaniasis, the current study establishes in vivo that anti-CD40 mAb treatment alone is sufficient to protect BALB/c mice from L. major infection and raises the possibility of utilizing this approach for vaccination strategies.
Assuntos
Anticorpos Monoclonais/uso terapêutico , Antígenos CD40/imunologia , Leishmania major/imunologia , Leishmaniose Cutânea/imunologia , Animais , Citocinas/biossíntese , Feminino , Imunidade Inata , Leishmaniose Cutânea/genética , Leishmaniose Cutânea/prevenção & controle , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Knockout , Linfócitos T/imunologia , Células Th1/metabolismoRESUMO
B cell knockout mice are unable to clear a primary erythrocytic infection of Plasmodium chabaudi chabaudi. However, the early acute infection is controlled to some extent, giving rise to a chronic relapsing parasitemia that can be reduced either by drug treatment or by adoptive transfer of B cells. Similar to mice rendered B-cell deficient by lifelong treatment with anti-mu antibodies, B cell knockout mice (muMT) retain a predominant CD4+ Th1-like response to malarial antigens throughout a primary infection. This contrasts with the response seen in control C57BL/6 mice in which the CD4+ T-cell response has switched to that characteristic of Th2 cells at the later stages of infection, manifesting efficient help for specific antibodies in vitro and interleukin 4 production. Both chloroquine and adoptive transfer of immune B cells reduced parasite load. However, the adoptive transfer of B cells resulted in a Th2 response in recipient muMT mice, as indicated by a relative increase in the precursor frequency of helper cells for antibody production. These data support the idea that B cells play a role in the regulation of CD4+ T subset responses.
Assuntos
Linfócitos B/imunologia , Malária/imunologia , Subpopulações de Linfócitos T/imunologia , Células Th1/imunologia , Células Th2/imunologia , Animais , Cloroquina/uso terapêutico , Eritrócitos/parasitologia , Feminino , Genes de Imunoglobulinas , Cadeias mu de Imunoglobulina/genética , Camundongos , Camundongos Knockout , Plasmodium chabaudi/imunologia , Pirimetamina/farmacologiaRESUMO
The heat stable antigen (HSA, or murine CD24) is a glycosyl phosphatidylinositol-linked surface glycoprotein expressed on immature cells of most, if not all, major hematopoietic lineages, as well as in developing neural and epithelial cells. It has been widely used to stage the maturation of B and T lymphocytes because it is strongly induced and then repressed again during their maturation. Terminally differentiated lymphocytes, as well as most myeloid lineages, are negative for HSA. Erythrocytes are an exception in that they maintain high levels of HSA expression. HSA on naive B cells has been shown to mediate cell-cell adhesion, while HSA on antigen-presenting cells has been shown to mediate a costimulatory signal important for activating T lymphocytes during an immune response. Here, we characterize mice that lack a functional HSA gene, constructed by homologous recombination in embryonic stem cells. While T-cell and myeloid development appears normal, these mice show a leaky block in B-cell development with a reduction in late pre-B and immature B-cell populations in the bone marrow. Nevertheless, peripheral B-cell numbers are normal and no impairment of immune function could be detected in these mice in a variety of immunization and infection models. We also observed that erythrocytes are altered in HSA-deficient mice. They show a higher, tendency to aggregate and are more susceptible to hypotonic lysis in vitro. In vivo, the mean half-life of HSA-deficient erythrocytes was reduced. When infected with the malarial parasite Plasmodium chabaudi chabaudi, the levels of parasite-bearing erythrocytes in HSA-deficient mice were also significantly elevated, but the mice were able to clear the infection with kinetics similar to wild-type mice and were immune to a second challenge. Thus, apart from alterations in erythrocytes and a mild block in B-cell development, the regulated expression of HSA appears to be dispensable for the maturation and functioning of those cell lineages that normally express it.
Assuntos
Antígenos CD/genética , Linfócitos B/patologia , Eritrócitos/patologia , Hematopoese/genética , Glicoproteínas de Membrana , Animais , Antígenos CD/biossíntese , Linfócitos B/imunologia , Linfócitos B/efeitos da radiação , Medula Óssea/patologia , Medula Óssea/efeitos da radiação , Cruzamento , Antígeno CD24 , Eritrócitos/imunologia , Eritrócitos/metabolismo , Feminino , Hematopoese/imunologia , Hematopoese/efeitos da radiação , Contagem de Linfócitos/efeitos da radiação , Malária/sangue , Malária/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Knockout , Plasmodium chabaudi/crescimento & desenvolvimento , Quimera por Radiação , Linfócitos T/imunologia , Linfócitos T/patologia , Linfócitos T/efeitos da radiaçãoRESUMO
Splenic CD4+NK1.1+ T cells have been shown to secrete large and transient amounts of IL-4 mRNA 90 min after i.v. injection of anti-CD3 Ab, suggesting that this novel subset of T cells may induce Th2 responses in the spleen by quickly providing IL-4 at the onset of an immune response. beta2-microglobulin-deficient (beta2m(o/o)) mice have been shown to contain strongly reduced numbers of NK1.1+ T cells and to be severely impaired in their capacity for rapid induction of IL-4 mRNA in response to anti-CD3, demonstrating that these cells are MHC class I dependent. To address the role of CD4+NK1.1+ T cells in the induction of Th2 responses against Leishmania major, we have dissected the onset and the outcome of the immune response elicited against the parasite in BALB/c-beta2m(o/o) mice and in anti-NK1.1-treated congenic BALB/c mice expressing the NK1.1 marker (BALB/c-NK1.1+). Both BALB/c-beta2m(o/o) and NK1.1-depleted BALB/c-NK1.1+ mice developed a progressive, nonhealing disease that was indistinguishable from wild-type mice. Upon infection, early induction of IL-4 mRNA in the lymph node was not affected in BALB/c-beta2m(o/o) and in NK1.1-depleted BALB/c-NK1.1+ mice, but was abrogated by injection of a CD4-depleting Ab. These data suggest that, in the lymph node, MHC class I-dependent CD4+NK1.1+ T cells do not play a major role in the generation of Th2 responses against L. major. To investigate whether the inability of NK1.1+ T cells to induce IL-4 production in the lymph node was specific to L. major Ag, mice were challenged with low doses of anti-CD3 Ab s.c. in the footpad. In contrast to the spleen, normal levels of IL-4 mRNA were expressed in the lymph nodes of BALB/c-beta2m(o/o) mice. Thus, the MHC class I-dependent CD4+NK1.1+ cell population that gives a rapid IL-4 response in the spleen appears not to contribute significantly to early induced IL-4 responses in the popliteal lymph nodes.
Assuntos
Antígenos/análise , Antígenos CD4/análise , Antígenos de Histocompatibilidade Classe I/fisiologia , Interleucina-4/biossíntese , Ativação Linfocitária , Proteínas/análise , Subpopulações de Linfócitos T/imunologia , Subpopulações de Linfócitos T/metabolismo , Células Th2/imunologia , Animais , Antígenos Ly , Antígenos de Superfície , Feminino , Lectinas Tipo C , Leishmania major/patogenicidade , Leishmaniose Cutânea/imunologia , Linfonodos/citologia , Linfonodos/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Subfamília B de Receptores Semelhantes a Lectina de Células NK , Fatores de TempoRESUMO
Mice deficient of mature B cells due to a targeted disruption of the transmembrane exon of the Ig mu-chain gene (mu-MT mice) can reduce a primary acute infection with the malaria parasite Plasmodium chabaudi chabaudi (AS strain) to low levels but are unable to eliminate parasites and instead develop chronic relapsing parasitemias. This model of B cell deficiency confirms previous findings using anti-mu-treated mice that B cells are required for final parasite clearance. Injection of B cells from immune donors into chronically infected mu-MT mice enabled them to clear their infection within 1 wk. When mu-MT mice that had been cured of their malaria infection by treatment with chloroquine were rechallenged with P. c. chabaudi (AS) they developed secondary infections of a magnitude similar to a primary infection, in contrast to wild-type mice in which a secondary challenge results only in a transient low patent parasitemia. These results suggest that B cell-dependent mechanisms play a crucial role in immunity to secondary infections. There is a pronounced expansion of gamma delta cells in the spleen of chronically infected mu-MT mice. After clearance of parasites in mu-MT mice either after adoptive transfer of immune B cells or by treatment with chloroquine, gamma delta T cells returned to levels observed in wild-type mice. This suggests that the expansion of gamma delta cells observed in mu-MT mice is due to the chronic persistence of parasites, rather than to the lack of B cells.
Assuntos
Linfócitos B/imunologia , Malária/imunologia , Plasmodium chabaudi , Animais , Anticorpos Antiprotozoários/administração & dosagem , Modelos Animais de Doenças , Feminino , Marcação de Genes , Cadeias mu de Imunoglobulina/genética , Imunoterapia Adotiva , Linfopenia/genética , Linfopenia/imunologia , Malária/genética , Malária/parasitologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Mutantes , Parasitemia/genética , Parasitemia/imunologia , Plasmodium chabaudi/imunologia , Receptores de Antígenos de Linfócitos T gama-delta/metabolismo , Subpopulações de Linfócitos T/imunologia , Fatores de TempoRESUMO
The molecular mechanisms involved in the regulation of gene expression by transforming growth factor-beta (TGF-beta) have been analyzed. We show that TGF-beta specifically induces the activity of the proline-rich trans-activation domain of CTF-1, a member of the CTF/NF-I family of transcription factors. A TGF-beta-responsive domain (TRD) in the proline-rich transcriptional activation sequence of CTF-1 was shown to mediate TGF-beta induction in NIH-3T3 cells. Mutagenesis studies indicated that this domain is not the primary target of regulatory phosphorylations, suggesting that the growth factor may regulate a CTF-1-interacting protein. A two-hybrid screening assay identified a nucleosome component, histone H3, as a specific CTF-1-interacting protein in yeast. Furthermore, the CTF-1 trans-activation domain was shown to interact with histone H3 in both transiently and stably transfected mammalian cells. This interaction requires the TRD, and it appears to be upregulated by TGF-beta in vivo. Moreover, point mutations in the TRD that inhibit TGF-beta induction also reduce interaction with histone H3. In vitro, the trans-activation domain of CTF-1 specifically contacts histone H3 and oligomers of histones H3 and H4, and full-length CTF-1 was shown to alter the interaction of reconstituted nucleosomal cores with DNA. Thus, the growth factor-regulated trans-activation domain of CTF-1 can interact with chromatin components through histone H3. These findings suggest that such interactions may regulate chromatin dynamics in response to growth factor signaling.
Assuntos
Proteínas Estimuladoras de Ligação a CCAAT , Proteínas de Ligação a DNA/metabolismo , Histonas/metabolismo , Prolina/metabolismo , Fatores de Transcrição/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Células 3T3 , Animais , Sequência de Bases , Cromatina/metabolismo , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/genética , Regulação da Expressão Gênica , Glutamina/metabolismo , Humanos , Camundongos , Dados de Sequência Molecular , Fatores de Transcrição NFI , Nucleossomos/metabolismo , Oligodesoxirribonucleotídeos , Mutação Puntual , Regiões Promotoras Genéticas , Fatores de Transcrição/química , Fatores de Transcrição/genéticaRESUMO
Interleukin(IL)-4 promotes the development of T helper (TH)2 cells, induces immunoglobulin class switching to IgG1 and is thought to be essential for switching to IgE. During a primary infection with the erythrocytic stages of Plasmodium chabaudi chabaudi, TH1 and TH2 cells specific for the parasite appear sequentially as infection progresses. To dissect the possible role of TH2 responses at the later stages of infection, mice with a targetted disruption of the IL-4 gene were infected with P. chabaudi. IL-4-deficient mice were able to control and clear a primary infection, although recrudescent parasitemias were significantly higher in these mice compared with wild-type littermates; demonstrating that IL-4 per se is not required for parasite elimination. To evaluate the actual impairment of TH2 functions in the absence of IL-4 in vivo during an infection with P. chabaudi; the cellular and humoral responses to the parasite generated in vitro and in vivo were compared in the two types of mice. Our data indicate that in vitro TH1 responses and ex vivo IL-12 mRNA levels were sustained in the IL-4-deficient mice compared with wild-type littermates. Correspondingly, TH2-associated cytokine mRNA such as IL-5 and IL-6, but not IL-10, were reduced early in infection in the deficient animals. However, these cytokines were expressed at comparable levels at the later stages of infection in both types of mice. Reflecting these differences in TH function, IgG1 responses were decreased in vitro and delayed in vivo, whereas IgG2a and IgG2b responses appeared earlier in vivo in the deficient mice. Strikingly, IgE secretion was not blocked in vivo in the deficient mice; the onset of the synthesis of IgE mRNA was delayed during infection and the amount of circulating IgE was five times lower than in the wild-type littermates after 5 weeks of infection. All these impairments of TH2-related activities were insufficient to affect parasite clearance in the deficient mice, probably due to the fact that such activities were only delayed and could take place normally at the later stages of infection.
Assuntos
Interleucina-4/deficiência , Malária/imunologia , Plasmodium chabaudi/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Animais , Anticorpos Antiprotozoários/imunologia , Formação de Anticorpos , Sequência de Bases , Linfócitos T CD4-Positivos/imunologia , Citocinas/genética , Primers do DNA/química , Feminino , Expressão Gênica , Isotipos de Imunoglobulinas/imunologia , Cooperação Linfocítica , Camundongos , Camundongos Knockout , Dados de Sequência Molecular , RNA Mensageiro/genética , Baço/imunologiaRESUMO
CD4+ T cells are necessary for a protective immune response against the erythrocytic stages of the malaria parasite Plasmodium chabaudi chabaudi AS. B cells are not required for control of early acute parasitaemias, but appear to be important for final clearance of the infection, most probably by producing specific antibodies against the parasite. However, immune sera and immune IgG are unable to replace the protective capacity of B cells in adoptive transfer of immunity to P. chabaudi AS. It is therefore conceivable that B cells are required to achieve protective immunity, not only as effector plasma cells, but because they may also play a second important role. We have recently suggested that B cells may regulate the Th response to P. chabaudi AS during a primary infection. We discuss here the possibility of a dual requirement of B cells in achieving protective immunity to P. chabaudi AS.
Assuntos
Linfócitos B/imunologia , Malária/imunologia , Plasmodium chabaudi/imunologia , Animais , Anticorpos Antiprotozoários/imunologia , Humanos , Camundongos , Camundongos Knockout , Linfócitos T Auxiliares-Indutores/imunologiaRESUMO
This review describes the role of cytokines produced by CD4+ T cells and macrophages in response to the erythrocytic stages of P. chabaudi chabaudi and other malaria infections in mice. Since virtually all compartments of the immune system are activated during the response against malaria, the variety of cytokines produced during infection is considerable. There is, however, a clear differential expression of different cytokines during primary infection. Th1-related cytokines are predominantly produced during the acute phase of infection, and lead mainly to the induction of macrophage-derived cytokines. This antibody-independent pathway is probably on the one hand, sufficient for parasite control early in infection via macrophage-associated inflammatory responses, but can, on the other hand, also lead to the pathological consequences of infection. As the infection progresses, the pattern of cytokine production shifts towards a Th2-like response. B cells play a crucial role in this process. A major consequence of this switch to a production of Th2-related cytokines later in infection would be the down-regulation of IFN-gamma-induced macrophage activation and the promotion of antibody production by mature B cells. This suggest that the mechanism of parasite control in the later stages of infection is predominantly antibody-dependent.
Assuntos
Citocinas/fisiologia , Malária/imunologia , Plasmodium/imunologia , Animais , Eritrócitos/parasitologia , Linfócitos/imunologia , Macrófagos/imunologia , CamundongosRESUMO
Mice depleted of B cells from birth by treatment with anti-mu antibodies can control but not clear an infection with the malaria parasite Plasmodium chabaudi chabaudi (AS). Splenic CD4+ T cells from these mice were unable to mount a significant Th2 response to the parasite in vitro as shown by much lower precursor frequencies of Th cells for antibody production and of IL-4-producing cells compared with the response of control-treated mice. CD4+ T cells of the anti-mu-treated mice which respond to antigens of P. chabaudi chabaudi maintained a Th1 phenotype throughout primary infection, in contrast to control mice in which a sequential appearance of Th1 and Th2 responses was observed. These data show that Th1 responses in anti-mu-treated mice are sufficient to control parasitemia but not to eliminate an infection. The data further suggest that depletion of B cells by treatment with anti-mu antibodies reduces the generation of the Th2 subset during a primary response to P. chabaudi chabaudi.