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1.
J Biotechnol ; 308: 141-147, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31866427

RESUMO

Substrate inhibition of enzymes is one of the main obstacles encountered frequently in industrial biocatalysis. Haloketone reductase SsCR was seriously inhibited by substrate 2,2',4'-trichloroacetophenone. In this study, two essential loops were found that have a relationship with substrate binding by conducting X-ray crystal structure analysis. Three key residues were selected from the tips of the loops and substituted with amino acids with lower hydrophobicity to weaken the hydrophobic interactions that bridge the two loops, resulting in a remarkable reduction of substrate inhibition. Among these variants, L211H showed a significant attenuation of substrate inhibition, with a Ki of 16 mM, which was 16 times that of the native enzyme. The kinetic parameter kcat/Km of L211H was 3.1 × 103 s-1 mM-1, showing the comparable catalytic efficiency to that of the wild-type enzyme (WT). At the substrate loading of 100 mM, the space time yield of variant L211H in asymmetric reduction of the haloketone was 3-fold higher than that of the WT.

2.
Cancer Imaging ; 19(1): 79, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31791422

RESUMO

BACKGROUND: Vena cava thrombus is one of the main clinical manifestations of locally aggressive renal cell carcinoma (RCC). Inferior vena cava (IVC) wall invasion and presence of bland thrombus could affect the surgical outcome. This study aims to assess the value of contrast-enhanced ultrasound (CEUS) in detecting wall invasion and differentiating bland thrombus from tumor thrombus during robot-assisted IVC thrombectomy for RCC. METHODS: The intraoperative CEUS findings of 60 patients with RCC accompanied by IVC tumor thrombus were retrospectively analyzed. The CEUS features were compared with the intra- and post-operative pathological findings. CEUS in patients with wall invasion showed that the tumor thrombus was enhanced synchronously with the IVC wall, and the continuity of the IVC wall was lost. In contrast, in patients without wall invasion, CEUS showed that the contrast agent could pass between the tumor thrombus and the IVC wall, and the continuity of IVC wall was good. Typically, contrast-enhanced perfusion was seen in tumor thrombus but not in bland thrombus. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of CEUS were statistically analyzed. RESULTS: The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of the typical enhancement mode of CEUS were 93.1, 93.5, 93.3, 93.1, and 93.5% in identifying wall invasion and 100, 96, 96.7, 83.3, and 100% in differentiating bland thrombus from tumor thrombus, respectively. There were excellent inter-observer agreements for identifying IVC wall invasion and differentiating bland thrombus from tumor thrombus with kappa coefficients of 0.90 and 0.97. CONCLUSIONS: The present study indicates that intraoperative CEUS plays an important role in robot-assisted IVC thrombectomy for RCC. It can detect wall invasion and differentiate bland thrombus from tumor thrombus, thus offering real-time information to the operator during surgery.

3.
Br J Radiol ; 92(1104): 20190634, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31613647

RESUMO

OBJECTIVE: The aim of this study was to examine the local myocardial segments in hypertrophic cardiomyopathy (HCM) by MRI T1 and T2 mapping, and to investigate how tissue remodeling correlates with structural and functional remodeling in HCM. METHODS: 47 patients with HCM and 19 healthy volunteers were enrolled in this study. All subjects underwent cardiac MRI at 3.0 T. Native T1 and T2 values, end-diastolic wall thickness (EDTH), and percentage of systolic wall thickening (PSWT) were assessed in the left ventricular segments according to the American Heart Association model. Myocardial segments were categorized as normal, non-hypertrophic, mild-hypertrophic, moderate-hypertrophic, and severe-hypertrophic based on EDTH. The difference among all five groups, and the correlation between native T1 and T2 values, EDTH, and PSWT were evaluated. RESULTS: Native T1 and T2 values were significantly elevated in both non-hypertrophic and hypertrophic segments of HCM patients compared to controls (both p < 0.001). PSWT was preserved in non-hypertrophic segments (p = 0.838), while significantly impaired (p < 0.001) in hypertrophic segments. Native T1 value of severe hypertrophic segments in HCM was significantly higher than segments of mild and moderate hypertrophy (p < 0.05). CONCLUSION: In HCM patients, the non-hypertrophic myocardial segments already demonstrated significantly elevated T1 and T2 values, despite normal wall thickness and preserved contraction function. The finding suggests that tissue remodeling may precede morphological and functional remodeling in HCM. MRI native T1 and T2 mapping can provide additional value for HCM diagnosis at an early stage. ADVANCES IN KNOWLEDGE: Myocardial tissue remodeling, as detected by MRI native T1 and T2 mapping, occurs earlier than morphological and functional changes in HCM patients.


Assuntos
Cardiomiopatia Hipertrófica/diagnóstico por imagem , Coração/diagnóstico por imagem , Imagem por Ressonância Magnética/métodos , Miocárdio/patologia , Adulto , Cardiomiopatia Hipertrófica/patologia , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
4.
J Environ Manage ; 246: 119-127, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31176177

RESUMO

In this study, the impact of COD/SO42- ratio in sewage on methane and hydrogen sulfide production in sewer biofilms was investigated by using three identical lab-scale gravity sewer systems. The results showed that the COD/SO42- played a key role in the competition between methanogenic archaea (MA) and sulfate reducing bacteria (SRB). Both the lowest methane and hydrogen sulfide production were obtained at COD/SO42- ratio of 6. The carbon transformation revealed that the activity of both MA and SRB was inhibited at this COD/SO42- ratio. Methanosarcina and Methanobacterium were the two dominant MA, while Desulfonema, Desulfotomaculum and Desulfovibrio were the dominant SRB in this case. The specific SRB activity measured by batch tests proved that acetate was mainly degraded by the MA, while propionate was the preferred substrate for the SRB.


Assuntos
Sulfeto de Hidrogênio , Microbiota , Carbono , Hidrogênio , Redes e Vias Metabólicas , Metano , Esgotos , Sulfatos
5.
Materials (Basel) ; 12(12)2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31226794

RESUMO

Nitrogen hybridization is an attractive way to enhance the wettability and electric conductivity of porous carbon, which increases the capacitance of carbon-based supercapacitor, however, there is lack of low-cost methods to prepare the nitrogen-doped porous carbon materials. Herein, a novel facile nitrogen-containing bio-phenolic resin was synthesized by polymerization of the carbamate bio-oil, Phenol and paraformaldehyde. As a precursor of nitrogen-doped porous carbon, the nitrogen-containing bio-phenol resin was activated by the one-step molten-salt method. The resultant nitrogen-doped porous carbon showed a high specific surface area up to 1401 m2·g-1. As a supercapacitor electrode, the nitrogen-doped porous carbons showed specific capacitance of 159 F·g-1 at 0.5 A·g-1. It also exhibited high cyclic stability with 94.8% retention of the initial specific capacitance over 1000 charge-discharge cycles at 1.0 A·g-1. The results suggest that these nitrogen-containing bio-phenol resin provide a new source of nitrogen-doped porous carbon for high-performance supercapacitor electrodes.

6.
Materials (Basel) ; 12(7)2019 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-30987389

RESUMO

Aged asphalt can enormously affect the performance of asphalt pavement and cause serious environmental hazards. Microcapsule-modified asphalt is one of the effective means to improve the anti-aging ability of asphalt. In this work, novel sustained-release microcapsules containing rejuvenator were prepared by the solvent evaporation method. The morphology of the microcapsules was characterized by scanning electron microscopy (SEM). The sustained-release properties of the microcapsules were investigated by static thermogravimetric analysis. The physical properties such as penetration, ductility, softening point, and Brookfield viscosity of the original asphalt and microcapsule-modified asphalt were studied. In addition, the viscoelasticity of the original asphalt and microcapsule-modified asphalt was investigated by means of a dynamic shear rheometer (DSR). The results show that the prepared microcapsules have a smooth surface and a complete encapsulation with an average particle size of 60 µm. After the heating treatment (above 140 °C), a large number of micropores were formed on the shell surface of microcapsules, which provided a structural basis for the sustained-release of rejuvenator. The release rate of the rejuvenator was obviously slowed down by the microcapsules. The aging behavior of sustained-release microcapsules containing rejuvenator-modified asphalt can be greatly improved. The enhanced anti-aging properties of sustained-release microcapsule-modified asphalt are attributed to the functions of the rejuvenator which can be slowly released from the micropores on the microcapsules' surface, after which the light components lost in the original asphalt can be supplemented.

7.
Materials (Basel) ; 12(3)2019 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-30717319

RESUMO

Potassium geopolymer (KGP) ceramics are synthesized by the molten salt method. Metakaolin changes to a potassium geopolymer through a reaction with potassium silicate at 80 °C/24 h. Potassium geopolymer, with a silicon to aluminum (Si/Al) molar ratio of 2, turns into a leucite ceramic in molten potassium salt (KCl) at 900 °C/6 h. X-Ray Diffraction analysis of the KGP treated by molten KCl salt shows the amorphous phase change to leucite crystal phase. A scanning electron microscope image of sintered KGP proves that the grain size of the leucite crystal decreases with soaking time. Compared with solid state sintering, liquid molten salt sintering KGP can be more easily formed into dense ceramics at lower temperatures.

8.
Medicine (Baltimore) ; 98(4): e14077, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30681564

RESUMO

RATIONALE: Congenital pulmonary dysplasia (CPD), a congenital lung anomaly, is a heterogeneous group of developmental disorders with diverse clinical and imaging manifestations, including pulmonary agenesis, pulmonary aplasia, and pulmonary hypoplasia. Patients with CPD always have recurrent respiratory tract infections, dyspnea, and/or wheezing. To the best of the author's knowledge, no case of CPD with mild to moderate hemoptysis has been reported so far. Considering the rarity of this presentation, we herein report a case of hemoptysis caused by CPD. PATIENT CONCERNS: We report on an 11-year-old girl who survived for 11 years with hypoplasia of the left lung without any associated complications except hemoptysis. DIAGNOSIS: Left pulmonary hypoplasia. INTERVENTIONS: The patient underwent left lower pulmonary lobe resection. OUTCOMES: The patient recovered favorably after the operation, and no complications were observed. The hemoptysis was controlled and the cough disappeared after the surgery. LESSONS: CPD is a life-threatening disorder rarely encountered in the clinic, which plays an important role in various pediatric respiratory diseases. Misdiagnosis or delayed diagnosis of pulmonary malformations results in unnecessary treatments and hospitalization. The patients should undergo pulmonary contrast-enhanced CT and bronchoscopy for accurate and timely diagnosis, followed by surgical treatment.


Assuntos
Pneumopatias/diagnóstico , Pulmão/anormalidades , Criança , Feminino , Hemoptise/etiologia , Humanos , Pneumopatias/complicações , Pneumopatias/cirurgia
9.
Graefes Arch Clin Exp Ophthalmol ; 256(11): 2103-2112, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30187128

RESUMO

PURPOSE: The aim of this study was to explore the pathological changes of the nasolacrimal duct in rabbits with experimentally induced obstructive dacryocystitis in correlation with lacrimal endoscopic findings. METHODS: The rabbit model of obstructive dacryocystitis was created by injecting 0.15 ml of self-curing resin into the lacrimal duct. The control group received 0.15 ml of normal saline. Within 16 weeks after the obstructive, lacrimal endoscopy and pathological examination of the nasolacrimal duct were conducted at different time points of 1, 2, 4, 8, and 16 weeks. RESULTS: In the control group, lacrimal endoscopy revealed pink and smooth mucosa; and the pathological analysis revealed an epithelial layer that was composed of superficial columnar cells and a deep basal epithelial layer. The experimental rabbits showed clinical manifestations of obstructive dacryocystitis a week after the injection of self-curing resin. At weeks 1 and 2, the lacrimal endoscopy showed mucosal hyperemia and hemorrhagic spots on the nasolacrimal duct; and the pathological features included epithelial cell swelling and inflammatory cell infiltration. At weeks 4 and 8, the experimental group showed alternatively red and white mucosa under the lacrimal endoscopy, and the pathological features included proliferative epithelium accompanied by papillary hyperplasia. At week 16, the experimental group showed pale and coarse mucosa and white membrane-like layer covering the mucosal surface, and the pathological features included epithelial necrosis, squamous metaplasia, and sub-epithelial fibrosis. CONCLUSION: The mucosa of the nasolacrimal duct showed different pathological features at different time points after lacrimal duct obstruction, which was well correlated with the endoscopic findings. It is possible to predict the pathological stages by the endoscopic observation in NLOD patients.


Assuntos
Dacriocistite/patologia , Modelos Animais de Doenças , Endoscopia , Obstrução dos Ductos Lacrimais/patologia , Ducto Nasolacrimal/patologia , Animais , Doença Crônica , Feminino , Fibrose , Masculino , Membrana Mucosa , Coelhos
10.
Exp Ther Med ; 16(4): 3303-3308, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30233676

RESUMO

Myocardial infarction is one of the most common human cerebrovascular conditions and frequently leads to ischemic stroke. Evidence has indicated that magnetic resonance imaging (MRI) is a potential method for the diagnosis of patients with cardiovascular injury. However, the efficacy of MRI in diagnosing patients with myocardial infarction requires to be improved. In the present study, a novel nano-size contrast agent, a chitosan/Fe3O4-enclosed albumin (CFEA), was introduced that was used to quantify blood volume and permeability in the infarcted myocardium. A total of 68 patients with suspected myocardial infarction were recruited to analyze the efficacy of MRI combined with CFEA (MRI-CFEA). All patients received diagnosis by MRI and MRI-CFEA. It was revealed that MRI-CFEA provided a higher signal intensity than MRI in the same patients. It was demonstrated that the diagnostic efficacy of MRI-CFEA for patients with myocardial infarction was higher than that of MRI (P<0.05). By MRI-CFEA, 50/68 of cases with myocardial infarction were diagnosed, providing a significantly higher diagnostic rate compared with the 38/68 of cases diagnosed by contrast-enhanced MRI (P<0.01). MRI-CFEA successfully discriminated the infarcted regions based on a decreased fractional blood volume and increased permeability-surface (PS) area product in the infarcted myocardium. A pharmacodynamics analysis indicated that CFEA was eliminated within 24 h in all individuals. In conclusion, the present study provided a novel method to diagnose infarcted myocardium for patients with myocardial infarction, providing an imaging biomarker for the assessment of endothelial dysfunction in the clinic.

11.
Genes (Basel) ; 9(8)2018 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-30061525

RESUMO

Non-invasive prediction of isocitrate dehydrogenase (IDH) genotype plays an important role in tumor glioma diagnosis and prognosis. Recently, research has shown that radiology images can be a potential tool for genotype prediction, and fusion of multi-modality data by deep learning methods can further provide complementary information to enhance prediction accuracy. However, it still does not have an effective deep learning architecture to predict IDH genotype with three-dimensional (3D) multimodal medical images. In this paper, we proposed a novel multimodal 3D DenseNet (M3D-DenseNet) model to predict IDH genotypes with multimodal magnetic resonance imaging (MRI) data. To evaluate its performance, we conducted experiments on the BRATS-2017 and The Cancer Genome Atlas breast invasive carcinoma (TCGA-BRCA) dataset to get image data as input and gene mutation information as the target, respectively. We achieved 84.6% accuracy (area under the curve (AUC) = 85.7%) on the validation dataset. To evaluate its generalizability, we applied transfer learning techniques to predict World Health Organization (WHO) grade status, which also achieved a high accuracy of 91.4% (AUC = 94.8%) on validation dataset. With the properties of automatic feature extraction, and effective and high generalizability, M3D-DenseNet can serve as a useful method for other multimodal radiogenomics problems and has the potential to be applied in clinical decision making.

12.
Acad Radiol ; 25(11): 1398-1404, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-29752156

RESUMO

RATIONALE AND OBJECTIVES: Spectral computed tomography (CT) imaging is widely used in the diagnosis of various cancers. This study aimed to analyze the characteristics of lung squamous cell carcinoma (SC) and adenocarcinoma (AC) using spectral CT imaging. METHODS: Sixty patients who were examined via spectral CT imaging and confirmed as having AC or SC via surgery and pathology were enrolled in this research project. A spectrum CT scanner was used, and both plain and enhanced CT scans were conducted to acquire spectral images. All patients' samples were used to detect the expression of thyroid transcription factor-1 (TTF-1) and epidermal growth factor receptor (EGFR) in cancer cells via immunohistochemical methods. RESULTS: Among the 27 cases with AC, 18 cases were identified as TTF-1 positive, 9 cases were found to be TTF-1 negative, 20 cases were confirmed as EGFR positive, and 7 cases were found to be EGFR negative. Among the 33 patients with SC, 6 cases were identified as TTF-1 positive, 27 cases were found to be TTF-1 negative, 19 cases were confirmed as EGFR positive, and 14 cases were found to be EGFR negative. No statistically significant differences were observed in normalized iodine concentration (NIC), K values, and calcium content between the TTF-1-positive and TTF-1-negative groups when considering patients. Statistically significant differences in NIC and K values were noted between the EGFR-positive and EGFR-negative groups among patients with AC, but no such difference was observed regarding calcium content. Significant differences in NIC, K values, and calcium content were observed between the EGFR-positive and EGFR-negative groups among patients with SC. CONCLUSIONS: In lung cancer cells, the parameters of spectral CT imaging, including NIC and K values, reflect the microvessel density and blood supply. Calcium content is an indicator of the growth status of lung SC.


Assuntos
Adenocarcinoma/diagnóstico por imagem , Carcinoma de Células Escamosas/diagnóstico por imagem , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/metabolismo , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Adulto , Idoso , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Receptores ErbB/metabolismo , Feminino , Humanos , Iodo/metabolismo , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Fator Nuclear 1 de Tireoide/metabolismo , Tomografia Computadorizada por Raios X
13.
J Thorac Dis ; 10(3): 1377-1385, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29707287

RESUMO

Background: Guidelines of the Global Initiative for Asthma recommend the use of peak expiratory flow (PEF) in the assessment and management of patients with asthma. However, normal PEF values for Chinese children have not been thoroughly investigated. Methods: This was a cross-sectional study of 3,169 healthy children aged 5-14 years from research centers in five cities of China: Guangzhou, Suzhou, Chengdu, Xi'an, and Beijing. We established pediatric reference values for PEF using a mini peak flow meter. PEF values recorded by the mini peak flow meter were compared with those obtained using a spirometer. Results: Height was the biometric variable with greatest correlation to PEF for both sexes. Significant differences were noted between males and females. The regression equation for boys was calculated as PEF =4.39× height (cm) -300.48 (R2 =0.76, P<0.001); for girls, this equation was PEF =4.13× height (cm) -278.04 (R2 =0.72, P<0.001). PEF values for Chinese children according to age were close to those of Irish, Turkish, and British children but were lower than those of children in Greece; PEF values according to height were similar to those of Turkish and Danish children but lower than values for children in Ireland. Conclusions: We established normal PEF values and developed predictive equations using linear regression analysis for Chinese children aged 5-14 years, while Greece and Ireland references were inappropriate for Chinese children.

14.
J Magn Reson Imaging ; 47(1): 91-96, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-28577335

RESUMO

PURPOSE: To investigate the application of whole-lesion histogram analysis of pharmacokinetic parameters for differentiating malignant from benign breast lesions on dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). MATERIALS AND METHODS: In all, 92 women with 97 breast lesions (26 benign and 71 malignant lesions) were enrolled in this study. Patients underwent dynamic breast MRI at 3T using a prototypical CAIPIRINHA-Dixon-TWIST-VIBE (CDT-VIBE) sequence and a subsequent surgery or biopsy. Inflow rate of the agent between plasma and interstitium (Ktrans ), outflow rate of agent between interstitium and plasma (Kep ), extravascular space volume per unit volume of tissue (ve ) including mean value, 25th/50th/75th/90th percentiles, skewness, and kurtosis were then calculated based on the whole lesion. A single-sample Kolmogorov-Smirnov test, paired t-test, and receiver operating characteristic curve (ROC) analysis were used for statistical analysis. RESULTS: Malignant breast lesions had significantly higher Ktrans , Kep , and lower ve in mean values, 25th/50th/75th/90th percentiles, and significantly higher skewness of ve than benign breast lesions (all P < 0.05). There was no significant difference in kurtosis values between malignant and benign breast lesions (all P > 0.05). The 90th percentile of Ktrans , the 90th percentile of Kep , and the 50th percentile of ve showed the greatest areas under the ROC curve (AUC) for each pharmacokinetic parameter derived from DCE-MRI. The 90th percentile of Kep achieved the highest AUC value (0.927) among all histogram-derived values. CONCLUSION: The whole-lesion histogram analysis of pharmacokinetic parameters can improve the diagnostic accuracy of breast DCE-MRI with the CDT-VIBE technique. The 90th percentile of Kep may be the best indicator in differentiation between malignant and benign breast lesions. LEVEL OF EVIDENCE: 4 Technical Efficacy Stage: 2 J. Magn. Reson. Imaging 2018;47:91-96.


Assuntos
Neoplasias da Mama/diagnóstico por imagem , Mama/diagnóstico por imagem , Meios de Contraste/química , Imagem por Ressonância Magnética/métodos , Adolescente , Adulto , Idoso , Biópsia , Feminino , Humanos , Interpretação de Imagem Assistida por Computador , Processamento de Imagem Assistida por Computador , Pessoa de Meia-Idade , Prognóstico , Curva ROC , Reprodutibilidade dos Testes , Estatísticas não Paramétricas , Adulto Jovem
15.
J Huazhong Univ Sci Technolog Med Sci ; 37(6): 938-942, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29270757

RESUMO

The clinical application of orbital magnetic resonance (MR) T2-mapping imaging in detecting the disease activity of Graves' ophthalmopathy (GO), and the predictive values of therapy response to intravenous glucocorticoid (ivGC) were investigated. Approved by the local institutional review board (IRB), 106 consecutive patients with GO were included in this prospective study. All subjects were divided into two groups according to the patients' clinical activity score (CAS): the CAS positive group (CAS ≥3) or the CAS negative group (CAS <3). T2 relaxation time of extraocular muscles (T2RT; ms) and the areas of four extra-ocular muscles (AEOMs; mm2) were measured by 3D T2-mapping MR sequence before and after methylprednisolone treatment, so as the CAS and some ophthalmic examinations including visual acuity, intra-ocular pressure, eyeball movement, diplopia and proptosis. In addition, 24 healthy volunteers were recruited as the control group. The mean T2RT and AEOMs in CAS positive group were higher than those in CAS negative group. Both CAS positive and negative groups had significantly higher mean T2RT and AEOMs than the control group (P<0.01). There was a positive correlation between T2RT and AEOMs values in GO patients, both of them had a positive correlation with CAS and the ophthalmic examinations. It was concluded that to evaluate the activity of GO, CAS was mostly related to inflammation symptoms of ocular surface, more than that, T2RT and AEOMs were also related to abnormal findings of the ophthalmic examinations including high ocular pressure, impaired eyeball movement, diplopia and proptosis. T2RT and AEOMs can reflex the inflammation state of ocular muscles better. CAS combined with 3D T2-mapping MR imaging could improve the sensitivity of detection of active GO so as the prediction and evaluation of the response to methylprednisolone treatment.


Assuntos
Oftalmopatia de Graves/diagnóstico por imagem , Oftalmopatia de Graves/tratamento farmacológico , Imagem por Ressonância Magnética/métodos , Metilprednisolona/uso terapêutico , Músculos Oculomotores/diagnóstico por imagem , Adolescente , Adulto , Idoso , Anti-Inflamatórios/uso terapêutico , Estudos de Casos e Controles , Movimentos Oculares , Feminino , Glucocorticoides/uso terapêutico , Oftalmopatia de Graves/fisiopatologia , Humanos , Pressão Intraocular , Masculino , Pessoa de Meia-Idade , Músculos Oculomotores/efeitos dos fármacos , Músculos Oculomotores/fisiopatologia , Estudos Prospectivos , Índice de Gravidade de Doença , Resultado do Tratamento , Acuidade Visual
16.
J Thorac Dis ; 9(11): 4538-4549, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29268524

RESUMO

Background: Although there are over 1.34 billion Chinese in the world, nationwide spirometric reference values for Chinese are unavailable, which is usually based on Caucasian conversion. The aim of this study was to establish spirometric reference values for Chinese with a national wide sample. Methods: We enrolled healthy non-smokers in 24 centers in Northeast, North, Northwest, Southwest, South, East and Central China from January 2007 to June 2010. Spirometry was performed according to American Thoracic Society and European Respiratory Society guidelines. Reference equations were established using the Lambda-Mu-Sigma (LMS) method for forced expiratory volume in 1 second (FEV1), forced vital capacity (FVC), FEV1/FVC, peak expiratory flow (PEF) and maximal midexpiratory flow (MMEF). Popular Caucasian reference values adjusted with ethnic conversion factors were validated with Chinese measured spirometry data. The present study also compared with other published Chinese equations for spirometry. Results: A total of 7,115 eligible individuals aged 4 to 80 years (50.9% females) were recruited. Reference equations against age and height by gender were established, including predicted values and lower limits of normal (LLNs). Validated with Chinese data, the mean percentage differences of Caucasian reference values adjusted with ethnic conversion factors were -10.2% to 1.8%, and the percentages of total subjects under LLNs were 0.1% to 8.9%. Compared with this study, the percentage differences of previous Chinese studies ranged from -17.8% to 11.4%, which were found to significantly overestimate or underestimate lung function. Conclusions: This study established new reference values for better interpretation of spirometry in Chinese aged 4 to 80 years, while Caucasian references with adjustment were inappropriate for Chinese.

17.
J Ophthalmol ; 2017: 3438041, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28717520

RESUMO

We established a chronic dacryocystitis model by injecting of 0.05, 0.1, and 0.15 ml self-curing resin via the lacrimal punctum in rabbits. Animals were randomized into four groups (n = 11 animals/group). The control group received 0.15 ml normal saline. Within three months postinjection, epiphora and eye discharge were observed. At the 90th day postlacrimal passage irrigation, CT dacryocystography was performed to find changes in the lacrimal image, and hematoxylin and eosin staining was made to identify pathological changes of the lacrimal sac. Three months postinjection, the rabbits in control group and those who received 0.05 and 0.1 ml self-curing resin failed to develop chronic dacryocystitis. However, 8/11 (72.7%) rabbits those received 0.15 ml self-curing resin were symptomatic and showed complete reflux in lacrimal passage irrigation, indicating the obstruction of the nasolacrimal duct. CT dacryocystography showed that the obstruction was present only in the animals with chronic dacryocystitis. Pathological examinations of chronic dacryocystitis also revealed significantly inflammatory changes, such as mucus epithelium thickening, irregular papillary proliferation, and submucosal fibrous deposition. Local injection of 0.15 ml self-curing resin can induce permanent obstruction of the nasolacrimal duct in rabbits and establish a model of chronic dacryocystitis.

18.
Sci Rep ; 7(1): 3117, 2017 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-28596583

RESUMO

Preoperative renal tumor subtype differentiation is important for radiology and urology in clinical practice. Pharmacokinetic data (K trans & V e, etc.) derived from dynamic contrast-enhanced MRI (DCE-MRI) have been used to investigate tumor vessel permeability. In this prospective study on DCE-MRI pharmacokinetic studies, we enrolled patients with five common renal tumor subtypes: clear cell renal cell carcinoma (ccRCC; n = 65), papillary renal cell carcinoma (pRCC; n = 12), chromophobic renal cell carcinoma (cRCC; n = 9), uroepithelial carcinoma (UEC; n = 14), and fat-poor angiomyolipoma (fpAML; n = 10). The results show that K trans of ccRCC, pRCC, cRCC, UEC and fpAML (0.459 ± 0.190 min-1, 0.206 ± 0.127 min-1, 0.311 ± 0.111 min-1, 0.235 ± 0.116 min-1, 0.511 ± 0.159 min-1, respectively) were different, but V e was not. K trans could distinguish ccRCC from non-ccRCC (pRCC & cRCC) with a sensitivity of 76.9% and a specificity of 71.4%, respectively, as well as to differentiate fpAML from non-ccRCC with a sensitivity of 100% and a specificity of 76.2%, respectively. Our findings suggest that DCE-MRI pharmacokinetics are promising for differential diagnosis of renal tumors, especially for RCC subtype characterization and differentiation between fpAML and non-ccRCC, which may facilitate the treatment of renal tumors.


Assuntos
Aumento da Imagem , Neoplasias Renais/diagnóstico por imagem , Neoplasias Renais/patologia , Imagem por Ressonância Magnética , Adulto , Antineoplásicos/farmacocinética , Antineoplásicos/uso terapêutico , Diferenciação Celular/efeitos dos fármacos , Feminino , Humanos , Neoplasias Renais/tratamento farmacológico , Neoplasias Renais/metabolismo , Imagem por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Curva ROC , Carga Tumoral
19.
PLoS One ; 12(3): e0172903, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28257494

RESUMO

Domestication has altered a variety of traits within the Eurasian perch (Perca fluviatilis), including phenotypic, physiological and behavioral traits of Eurasian perch (Perca fluviatilis). Little is known, however, about the genetic changes between domesticated and wild Eurasian perch. In this study, we assembled a high-quality de novo reference transcriptome and identified differentially expressed genes between wild and domesticated Eurasian perch. A total of 113,709 transcripts were assembled, and 58,380 transcripts were annotated. Transcriptomic comparison revealed 630 differentially expressed genes between domesticated and wild Eurasian perch. Within domesticated Eurasian perch there were 412 genes that were up-regulated including MHCI, MHCII, chia, ighm within immune system development. There were 218 genes including try1, ctrl, ctrb, cela3b, cpa1 and cpb1, which were down-regulated that were associated with digestive processes. Our results indicated domestication drives the changes of immune and digestive system of Eurasian perch. Our study not only provide valuable genetic resources for further studies in Eurasian perch, but also provide novel insights into the genetic basis of physiological changes in Eurasian perch during domestication process.


Assuntos
Domesticação , Percas/genética , Transcriptoma/genética , Animais , Sistema Digestório , Regulação da Expressão Gênica/genética , Sistema Imunitário , Percas/fisiologia , Estresse Fisiológico/genética
20.
Sci Rep ; 6: 29146, 2016 07 06.
Artigo em Inglês | MEDLINE | ID: mdl-27380733

RESUMO

Pharmacokinetic parameters derived from dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) have been increasingly used to evaluate the permeability of tumor vessel. Histogram metrics are a recognized promising method of quantitative MR imaging that has been recently introduced in analysis of DCE-MRI pharmacokinetic parameters in oncology due to tumor heterogeneity. In this study, 21 patients with renal cell carcinoma (RCC) underwent paired DCE-MRI studies on a 3.0 T MR system. Extended Tofts model and population-based arterial input function were used to calculate kinetic parameters of RCC tumors. Mean value and histogram metrics (Mode, Skewness and Kurtosis) of each pharmacokinetic parameter were generated automatically using ImageJ software. Intra- and inter-observer reproducibility and scan-rescan reproducibility were evaluated using intra-class correlation coefficients (ICCs) and coefficient of variation (CoV). Our results demonstrated that the histogram method (Mode, Skewness and Kurtosis) was not superior to the conventional Mean value method in reproducibility evaluation on DCE-MRI pharmacokinetic parameters (K( trans) &Ve) in renal cell carcinoma, especially for Skewness and Kurtosis which showed lower intra-, inter-observer and scan-rescan reproducibility than Mean value. Our findings suggest that additional studies are necessary before wide incorporation of histogram metrics in quantitative analysis of DCE-MRI pharmacokinetic parameters.


Assuntos
Carcinoma de Células Renais/diagnóstico por imagem , Meios de Contraste/farmacocinética , Neoplasias Renais/diagnóstico por imagem , Imagem por Ressonância Magnética , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes
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