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1.
Child Dev ; 95(1): 191-207, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-37551445

RESUMO

This study examined transitions in resilience profiles and the role of caregiver risk and protective factors in resilience transition probabilities over 18 months among children involved with the child welfare system, using latent profile analysis and latent transition analysis. The sample included 486 children (48% female, baseline Mage = 3.49). There were three resilience profiles at Time 1 (19.9% low emotional behavioral, 26.1% low cognitive, 54.0% multidomain) and two profiles at Time 2 (18.9% low emotional behavioral, 81.1% multidomain). Caregiver mental health problems were negatively associated with membership in the multidomain resilience group at Time 1. Higher levels of cognitive stimulation were associated with initial and continued membership in the multidomain resilience group. Implications for resilient child development are discussed.


Assuntos
Resiliência Psicológica , Criança , Humanos , Feminino , Pré-Escolar , Masculino , Proteção da Criança , Emoções , Desenvolvimento Infantil , Fatores de Proteção
2.
Pharmaceuticals (Basel) ; 16(12)2023 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-38139854

RESUMO

The species Chenopodium quinoa Willd. and Amaranthus hybridus L. are Andean staples, part of the traditional diet and gastronomy of the people of the highlands of Colombia, Ecuador, Peru, Bolivia, northern Argentina and Chile, with several ethnopharmacological uses, among them anticancer applications. This review aims to present updated information on the nutritional composition, phytochemistry, and antimicrobial and anticancer activity of Quinoa and Amaranth. Both species contribute to food security due to their essential amino acid contents, which are higher than those of most staples. It is highlighted that the biological activity, especially the antimicrobial activity in C. quinoa, and the anticancer activity in both species is related to the presence of phytochemicals present mostly in leaves and seeds. The biological activity of both species is consistent with their phytochemical composition, with phenolic acids, flavonoids, carotenoids, alkaloids, terpenoids, saponins and peptides being the main compound families of interest. Extracts of different plant organs of both species and peptide fractions have shown in vitro and, to a lesser degree, in vivo activity against a variety of bacteria and cancer cell lines. These findings confirm the antimicrobial and anticancer activity of both species, C. quinoa having more reported activity than A. hybridus through different compounds and mechanisms.

3.
Antioxidants (Basel) ; 12(11)2023 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-38001856

RESUMO

Several plants of the genus Tragia L. have shown antibacterial, fungicidal, and antiproliferative activity, among other types of activities; however, most species of the genus have not been investigated. Tragia volubilis L. is native to tropical America and Africa, and although it has been reported as medicinal in the literature, it has not been thoroughly investigated. In this study, the phytochemical screening, isolation, and identification of compounds and the determination of the antioxidant activity of the aqueous extract of Tragia volubilis L. and its partitions were carried out. Ethyl acetate and n-butanol partitions of the extract present high antioxidant activity according to the Antioxidant Activity Index. Due to their activity, these partitions were tested on RKO cells as a representative model, both individually and in combination with Doxorubicin. It was found that the partitions significantly reduced the effect of Doxorubicin, as well as the expression of proteins involved in DNA damage and cell death. While the reduction of the chemotherapeutic effect of Doxorubicin on tumor cells may not be a desired outcome in therapeutic settings, the findings of the study are valuable in revealing the antioxidant potential of Tragia volubilis L. and its partitions. This highlights the importance of carefully regulating the application of antioxidants, especially in the context of cancer chemotherapy.

4.
ACS Omega ; 8(45): 42632-42646, 2023 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-38024734

RESUMO

Eight Schiff bases, synthesized by the reaction of 4-aminoantipyrine with different cinnamaldehydes, were studied in the solid state by using vibrational spectroscopy (IR) and X-ray diffraction techniques. The analysis was extended to the solution phase through ultraviolet-vis, fluorescence spectroscopy, and cyclic voltammetry. Finally, the crystal structures of four compounds (3b, 3d, 3g, and 3h) were determined and studied. In addition to the experimental study, theoretical calculations using the semiempirical method PM6/ZDO were performed to understand better the compound's molecular properties, UV-vis, and infrared spectra. The primary difference is the angular conformation of the terminal phenyl rings around the corresponding linking C-N and C-C σ-bonds. Furthermore, as a result of extended bonding, the > C=N- azomethine group-containing Cpyr-N=(CH)-(CR)=(CH)-Cbz chain (with R=H for 3b, 3d, and 3h, and R=CH3 for 3g) is planar, nearly coplanar, with the mean plane of the pyrazole ring. Hirshfeld surface (HS) analysis was used to investigate the crystal packing and intermolecular interactions, which revealed that intermolecular C-H···O and C-H···N hydrogen bonds, π···π stacking, and C-H···π and C=O···π interactions stabilize the compounds. The energy contributions to the lattice energies of potential hydrogen bonds were primarily dispersive and repulsive. All derivatives were tested in vitro on LPS-stimulated mouse macrophages to assess their ability to suppress the LPS-induced inflammatory responses. Only a slight reduction in the level of NO production was found in activated macrophages treated with 3h. Additionally, the derivatives were tested for antimicrobial activity against several clinical bacteria and fungi strains, including three biofilm-forming microorganisms. Nevertheless, only Schiff base 3f showed interesting antibacterial activities with minimum inhibitory concentration (MIC) values as low as 15.6 µM against Enterobacter gergoviae. On the other hand, Schiff base 3f and, to a lesser extent, 3b and 3h showed antifungal activity against clinical isolates of Candida. The lowest MIC value was for 3f against Candida albicans (15.6 µM). It is interesting to note that the same Schiff bases exhibit the highest activity in both biological evaluations.

5.
Plants (Basel) ; 12(17)2023 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-37687385

RESUMO

Several species from the genus Tragia L. in the family Euphorbiaceae are part of the ethnomedicine of traditional cultures, and have a variety of uses. Tragia volubilis L. is a species spread through tropical America and Africa with several ethnomedical uses, particularly for wound healing and reproductive issues. In this study, we assess the phytochemical composition and antioxidant activity of the methanolic extract of the aerial parts of T. volubilis collected in southern Ecuador. The phytochemical screening of the extract shows the preliminary presence of carbohydrates, alkaloids, flavonoids, and tannins. The extract shows an Antioxidant Activity Index of 1.14, interpreted as strong antioxidant activity. Four flavonoid compounds were isolated through chromatographic procedures and identified through NMR spectroscopy: avicularin, quercitrin, afzelin, and amentoflavone. The biological activity of these compounds matches the ethnopharmacological uses of the species. This is the first phytochemical study of T. volubilis and supports its traditional medicinal uses.

6.
Violence Against Women ; 29(15-16): 3302-3324, 2023 12.
Artigo em Inglês | MEDLINE | ID: mdl-37545376

RESUMO

This study explored risk and protective factors for resilience in survivors of sex trafficking from the survivors' perspectives. In-depth interviews were conducted with 16 participants recruited from survivor-led advocacy agencies and social service agencies that provide trafficking-specific services. Two overarching themes emerged from data analysis: (a) deep connections to self and others facilitate resilience and (b) detrimental "help" threatens survivors' resilience. Our findings suggest that it may be important for service providers to assist survivors build healthy relationships with themselves and others to promote survivors' resilience. Further, our results highlight the importance of offering more inclusive, survivor-centered, and trauma-informed services.


Assuntos
Tráfico de Pessoas , Humanos , Fatores de Proteção , Sobreviventes , Pesquisa Qualitativa , Serviço Social
7.
Plants (Basel) ; 12(4)2023 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-36840306

RESUMO

Andean peatlands are important carbon reservoirs for countries in the northern Andes and have a unique diversity. Peatland plant diversity is generally related to hydrology and water chemistry, and the response of the vegetation in tropical high-elevation peatlands to changes in elevation, climate, and disturbance is poorly understood. Here, we address the questions of what the main vegetation types of peat-forming vegetation in the northern Andes are, and how the different vegetation types are related to water chemistry and pH. We measured plant diversity in 121 peatlands. We identified a total of 264 species, including 124 bryophytes and 140 vascular plants. We differentiated five main vegetation types: cushion plants, Sphagnum, true mosses, sedges, and grasses. Cushion-dominated peatlands are restricted to elevations above 4000 m. Variation in peatland vegetation is mostly driven be elevation and water chemistry. Encroachment of sedges and Sphagnum sancto-josephense in disturbed sites was associated with a reduction in soil carbon. We conclude that peatland variation is driven first by elevation and climate followed by water chemistry and human disturbances. Sites with higher human disturbances had lower carbon content. Peat-forming vegetation in the northern Andes was unique to each site bringing challenges on how to better conserve them and the ecosystem services they offer.

8.
Am J Obstet Gynecol MFM ; 5(2): 100802, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36372188

RESUMO

BACKGROUND: There are 3 treatment options for placenta accreta spectrum: cesarean delivery with hysterectomy, expectant management, and uterine-sparing surgical techniques. One-step conservative surgery is the most extensively described conservative surgical technique, and it has extensive evidence supporting its usefulness; however, few groups apply it, most likely because of the misconception that it is a complex procedure that requires extensive training and is applicable to only a few patients. OBJECTIVE: This study aimed to evaluate the clinical outcomes of patients undergoing one-step conservative surgery in 4 placenta accreta spectrum reference hospitals and provided detailed steps for successfully applying this type of surgery. STUDY DESIGN: This was a multicenter, descriptive, prospective study that described the outcomes of patients with placenta accreta spectrum treated in 4 reference hospitals for this condition. The patients were divided into those managed with one-step conservative surgery and those managed with cesarean delivery and hysterectomy. RESULTS: Overall, 75 patients were included. One-step conservative surgery was possible in 85.3% of placenta accreta spectrum cases (64 patients). Intraoperative staging and placenta accreta spectrum topographic classification allowed for the selection of one-step conservative surgery candidates. The clinical outcomes of the 2 groups were similar, except for the frequency of transfusions (81.8% in the cesarean delivery and hysterectomy group vs 67.2% in the one-step conservative surgery group) and vascular interventions (27.3% in the cesarean delivery and hysterectomy group vs 4.7% in the one-step conservative surgery group), which were both higher in patients who underwent hysterectomy. In addition, the operation time was shorter in the one-step conservative surgery group (164.4 minutes vs 216.5 minutes). CONCLUSION: One-step conservative surgery is a valid procedure in most patients with placenta accreta spectrum. It is an applicable technique even in scenarios with limited resources. However, its safe application requires knowledge of the topographic classification and the application of intraoperative staging.


Assuntos
Placenta Acreta , Gravidez , Feminino , Humanos , Placenta Acreta/diagnóstico , Placenta Acreta/epidemiologia , Placenta Acreta/cirurgia , Estudos Prospectivos , Útero/cirurgia , Cesárea/métodos , Histerectomia/métodos
9.
Ginecol. obstet. Méx ; 91(5): 291-298, ene. 2023. tab
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1506263

RESUMO

Resumen OBJETIVO: Describir los factores asociados con la aceptación de la vacuna contra SARS-CoV-2 en embarazadas peruanas. MATERIALES Y MÉTODOS: Estudio observacional, analítico, transversal y retrospectivo efectuado en una muestra de pacientes embarazadas atendidas en el Centro Materno Infantil Santa Anita, Lima, Perú, seleccionadas por muestreo aleatorio simple entre los meses de enero a junio del 2022. RESULTADOS: Se analizaron 315 embarazadas. La proporción de aceptación de la vacuna contra SARS-CoV-2 fue del 58.1%. Del total de inmunizadas, 96.2% contaba con dos dosis y 3.8% solo una. El principal motivo por el que solo recibieron una dosis fue haber enfermado de COVID-19 y considerar que con era suficiente (42.8%). El motivo principal para no vacunarse fue el temor a que el biológico dañara a su hijo (40.1%). Los factores asociados con el trabajo dependiente obtuvieron una razón de prevalencia (Rp) de 2.18 (IC95%: 1.13-4.18; p = 0.019) y la infección previa por SARS-CoV-22 una Rp = 2.41 (IC95%: 1.03-5-59; p = 0.040). CONCLUSIÓN: Más de la tercera parte de las embarazadas no aceptó la vacunación contra el SARS-CoV-2. Los factores asociados con la aceptación de la vacuna fueron el antecedente de infección SARS-CoV-2 y realizar un trabajo dependiente.


Abstract OBJECTIVE: We describe the acceptance of the SARS-CoV-2 vaccine in Peruvian pregnant women and associated factors during 2022. METHODS: Observational, analytical, cross-sectional and retrospective study with a sample of 315 pregnant women selected by simple random sampling, January to June 2022. RESULTS: The acceptance rate of the SARS-CoV-2 vaccine was 58.1%. Of the total number of immunized women, 96.2% had two doses and 3.8% had only one dose; the main reason for having only one dose was having been ill with COVID-19 and one dose was sufficient (42.8%) while the main reason for not having any dose was the fear that the vaccine would harm the baby (40.1%). Regarding associated factors dependent work obtained a Prevalence Ratio (Rp= 2.18) CI95% [1.13-4.18; p=0.019] and previous SARS-CoV-22 infection an Rp= 2.41 CI95% [1.03-5.59; p=0.040]. CONCLUSION: more than one third of pregnant women did not accept vaccination against SARS-CoV-2; factors associated with acceptance of vaccination against COVID-19 were a history of SARS-CoV-2 infection and performing dependent work.

10.
Plants (Basel) ; 11(22)2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36432863

RESUMO

A pre-Columbian staple, Bactris gasipaes Kunth. is a palm tree domesticated around 4000 years ago, so appreciated that a Spanish chronicler wrote in 1545, "only their wives and children were held in higher regard" by the Mesoamerican natives. The peach palm is an integral part of the foodways and gastronomy of Ecuador, Colombia, Bolivia, Peru, Brazil, and other tropical American countries; meanwhile, it is almost unknown in the rest of the world, except for hearts of palm. Although abundant, the species faces anthropogenic threats. The purpose of this study is to describe and summarize the physicochemical, nutritional, and bioactive characteristics of the peach palm and its two main alimentary products: hearts of palm and fruits, highlighting the functional and antioxidant potential of the latter, showing both ancestral and modern uses. There is active research on peach palm products and coproducts that aim for better, more sustainable uses of its traditional and recently found properties. The review and presentation of studies on this strategically relevant species can motivate the protection of endangered populations and stimulate new lines of research to advance development in the food, pharmaceutical, and cosmetic industries, with fair trade, sustainable development goals, and adaptation to climate change in mind.

12.
Artigo em Inglês | MEDLINE | ID: mdl-35627523

RESUMO

This study aimed to examine the association between early childhood resilience profiles and later school outcomes (academic achievement and school involvement) among children in the U.S. child welfare system. This study compared 827 children aged 3-5 years in three latent profile groups (poor emotional and behavioral resilience, low cognitive resilience, and multi-domain resilience) to their baseline profiles using data from the National Survey of Child and Adolescent Well-Being (NSCAW-II). At the three-year follow-up, children with low emotional and behavioral resilience profiles and children with the multi-domain resilience profile had significantly higher basic reading skills, reading comprehension, and math reasoning compared to children with low scores on the cognitive resilience profile. Furthermore, children with the multi-domain resilience profile had significantly higher levels of emotional school engagement than did those with the low emotional and behavioral resilience profile and considerably higher levels of behavioral school engagement compared to those with the low cognitive resilience profile. The findings highlight the persistent effects of early resilience into the later childhood years. Moreover, our results suggest the need for early identification of and intervention for children with low cognitive or emotional/behavioral resilience during the preschool years to promote academic success and school engagement during the school-age years.


Assuntos
Sucesso Acadêmico , Proteção da Criança , Adolescente , Criança , Proteção da Criança/psicologia , Pré-Escolar , Escolaridade , Emoções , Humanos , Instituições Acadêmicas
13.
Plants (Basel) ; 11(9)2022 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-35567253

RESUMO

Xylosma G. Forst. is a genus of plants belonging to the Salicaceae family with intertropical distribution in America, Asia, and Oceania. Of the 100 accepted species, 22 are under some level of conservation risk. In this review, around 13 species of the genus used as medicinal plants were found, mainly in Central and South America, with a variety of uses, among which antimicrobial is the most common. There is published research in chemistry and pharmacological activity on around 15 of the genus species, centering in their antibacterial and fungicidal activity. Additionally, a variety of active phytochemicals have been isolated, the most representative of which are atraric acid, xylosmine and its derivatives, and velutinic acid. There is still ample field for the validation and evaluation of the activity of Xylosma extracts, particularly in species not yet studied, and concerning uses other than antimicrobial and for the identification and evaluation of their active compounds.

14.
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1385253

RESUMO

RESUMEN: En los hospitales públicos chilenos, los pacientes desdentados mandibulares pueden ser tratados con una Sobredentadura-Mucosoportada, Implanto-Retenida (SMIR). Para tal fin, se adaptaron los lineamientos del plan piloto "Programa de Prestaciones Valoradas. Odontología 2012 Ministerio de Salud", en ausencia de una Guía Ministerial. El objetivo de esta serie de casos fue evaluar si la implementación y adaptación del plan piloto del tratamiento con SMIR mejoró la calidad de vida de los pacientes. Se evaluaron sobrevida, éxito implantario, e impacto en la calidad de vida. Los resultados indican una sobrevida y éxito implantario del 100% y una mejora de la calidad de vida. Conclusión: el tratamiento con SMIR mejora la calidad de vida de los pacientes, en el servicio público.


ABSTRACT: In Chilean public hospitals, patients with Complete Edentulous Mandible are treated with Implant-Retained and Mucosa-supported Overdenture (IRMO). For this purpose, the guidelines of the pilot plan "Valued Benefits Program in Dentistry 2012, Ministry of Health" have been adapted. The aim of this study was to evaluate whether these adapted protocols have been successful. We measured implant survival and success, and impact on quality of life with OHIP-14 criteria. Results indicate 100% implant survival and success and improved quality of life. Conclusion: IRMO improves patients' quality of life in public services.

15.
Artigo em Inglês | MEDLINE | ID: mdl-35055823

RESUMO

In the United States, racial/ethnic and sexual youth and young adults (YYA) of color are disproportionately affected by HIV. Subsequently, YYA experience HIV stigma and engage in increased risk behaviors and reduced HIV testing. HIV communication has been identified as a potential buffer to HIV stigma, resulting in health-seeking behaviors, such as HIV testing. In this study, we respond to a meaningful gap in the literature by examining different types of HIV communication and their impact on HIV stigma and HIV testing in a diverse sample of YYA. We analyzed secondary data from the Kaiser Family Foundation National Survey of Teens and Young Adults on HIV/AIDS. A 40-question, web-based survey was conducted with 1437 youth (ages 15-24). Recruitment included a dual sampling method from households with: (1) listed phone numbers, (2) unlisted phone numbers, (3) telephones, (4) no telephone, and (5) only cell phone access. The purpose of the survey was to establish participants' HIV knowledge, communication, experiences, and testing behaviors. Findings suggested an association between intimate-partner HIV communication, increased HIV testing, and reduced HIV stigma. We also identified differentials in HIV testing and stigma based on gender, income, age, and sexual minority status, explained by HIV communication. Further research is needed that examines ways to use intimate-partner HIV communication to reduce stigma and increase HIV testing among YYA of different sociodemographic characteristics and sexual orientations.


Assuntos
Síndrome da Imunodeficiência Adquirida , Infecções por HIV , Adolescente , Adulto , Comunicação , Infecções por HIV/diagnóstico , Infecções por HIV/epidemiologia , Humanos , Comportamento Sexual , Parceiros Sexuais , Estigma Social , Estados Unidos , Adulto Jovem
16.
Glob Chang Biol ; 28(9): 3110-3144, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-34967074

RESUMO

Research in global change ecology relies heavily on global climatic grids derived from estimates of air temperature in open areas at around 2 m above the ground. These climatic grids do not reflect conditions below vegetation canopies and near the ground surface, where critical ecosystem functions occur and most terrestrial species reside. Here, we provide global maps of soil temperature and bioclimatic variables at a 1-km2 resolution for 0-5 and 5-15 cm soil depth. These maps were created by calculating the difference (i.e. offset) between in situ soil temperature measurements, based on time series from over 1200 1-km2 pixels (summarized from 8519 unique temperature sensors) across all the world's major terrestrial biomes, and coarse-grained air temperature estimates from ERA5-Land (an atmospheric reanalysis by the European Centre for Medium-Range Weather Forecasts). We show that mean annual soil temperature differs markedly from the corresponding gridded air temperature, by up to 10°C (mean = 3.0 ± 2.1°C), with substantial variation across biomes and seasons. Over the year, soils in cold and/or dry biomes are substantially warmer (+3.6 ± 2.3°C) than gridded air temperature, whereas soils in warm and humid environments are on average slightly cooler (-0.7 ± 2.3°C). The observed substantial and biome-specific offsets emphasize that the projected impacts of climate and climate change on near-surface biodiversity and ecosystem functioning are inaccurately assessed when air rather than soil temperature is used, especially in cold environments. The global soil-related bioclimatic variables provided here are an important step forward for any application in ecology and related disciplines. Nevertheless, we highlight the need to fill remaining geographic gaps by collecting more in situ measurements of microclimate conditions to further enhance the spatiotemporal resolution of global soil temperature products for ecological applications.


Assuntos
Ecossistema , Solo , Mudança Climática , Microclima , Temperatura
17.
Plants (Basel) ; 10(12)2021 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-34961188

RESUMO

Tragia L. is a genus of plants belonging to the Euphorbiaceae family with worldwide intertropical distribution, composed of more than 150 species. In this literature review, 26 species of the genus used as medicinal plants were found, mainly in East Africa and the Indian subcontinent, with a variety of uses among which antibacterial, anti-inflammatory, anticancer and reproductive health are most common. Research has been done on a few of the species, mostly those of the Old World, with emphasis on four of them: Tragia involucrata Linn., Tragia spathulata Benth., Tragia benthamii Baker and Tragia plukenetii Radcl.-Sm., confirming several ethnomedicinal claims. Moreover, a variety of active phytochemicals have been isolated, mainly ethers, hydrocarbons, flavonoids and sterols. There is ample field for the evaluation of the activity of Tragia extracts and essential oils and the identification of their active compounds, particularly of the New World species, for which there is still very little research.

18.
Molecules ; 26(16)2021 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-34443548

RESUMO

This work focuses on the search and development of drugs that may become new alternatives to the commercial drugs currently available for treatment of leishmaniasis. We have designed and synthesized 12 derivatives of bis(spiropyrazolone)cyclopropanes. We then characterized their potential application in therapeutic use. For this, the in vitro biological activities against three eukaryotic models-S. cerevisiae, five cancer cell lines, and the parasite L. mexicana-were evaluated. In addition, cytotoxicity against non-cancerous mammalian cells has been evaluated and other properties of interest have been characterized, such as genotoxicity, antioxidant properties and, in silico predictive adsorption, distribution, metabolism, and excretion (ADME). The results that we present here represent a first screening, indicating two derivatives of bis(spiropyrazolone)cyclopropanes as good candidates for the treatment of leishmaniasis. They have good specificity against parasites with respect to mammalian cells.


Assuntos
Antiprotozoários/síntese química , Antiprotozoários/farmacologia , Ciclopropanos/síntese química , Ciclopropanos/farmacologia , Leishmaniose/tratamento farmacológico , Animais , Antiprotozoários/química , Antiprotozoários/uso terapêutico , Linhagem Celular , Técnicas de Química Sintética , Ciclopropanos/química , Ciclopropanos/uso terapêutico , Desenho de Fármacos , Humanos , Leishmania/efeitos dos fármacos , Relação Estrutura-Atividade
19.
BMC Chem ; 15(1): 38, 2021 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-34082794

RESUMO

BACKGROUND: Pyrazoles have attracted particular attention due to the diverse biological activities associated with this heterocyclic system, and some have been shown to be cytotoxic to several human cell lines. Several drugs currently on the market have this heterocycle as the key structural motif, and some have been approved for the treatment of different types of cancer. RESULTS: 4,4'-(Arylmethylene)bis(1H-pyrazol-5-ols) derivatives 3a-q were synthetized by a three components reaction of 3-methyl-1-phenyl-5-pyrazolone (1) with various benzaldehydes 2 catalyzed by sodium acetate at room temperature. The structures of all synthesized compounds were characterized by physicochemical properties and spectral means (IR and NMR) and were evaluated for their radical scavenging activity by DPPH assay and tested in vitro on colorectal RKO carcinoma cells in order to determine their cytotoxic properties. All 4,4'-(arylmethylene)bis(1H-pyrazol-5-ols) derivatives 3a-q were synthetized in high to excellent yield, and pure products were isolated by simple filtration. All compounds have good radical scavenging activity, and half of them are more active than ascorbic acid used as standard. CONCLUSION: Several derivatives proved to be cytotoxic in the RKO cell line. In particular, compound 3i proved to be a very potent scavenger with an IC50 of 6.2 ± 0.6 µM and exhibited an IC50 of 9.9 ± 1.1 µM against RKO cell. Autophagy proteins were activated as a survival mechanism, whereas the predominant pathway of death was p53-mediated apoptosis.

20.
Glob Chang Biol ; 26(12): 7112-7127, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32902066

RESUMO

Global climate and land use change are causing woody plant encroachment in arctic, alpine, and arid/semi-arid ecosystems around the world, yet our understanding of the belowground impacts of this phenomenon is limited. We conducted a globally distributed field study of 13 alpine sites across four continents undergoing woody plant encroachment and sampled soils from both woody encroached and nearby herbaceous plant community types. We found that woody plant encroachment influenced soil microbial richness and community composition across sites based on multiple factors including woody plant traits, site level climate, and abiotic soil conditions. In particular, root symbiont type was a key determinant of belowground effects, as Nitrogen-fixing woody plants had higher soil fungal richness, while Ecto/Ericoid mycorrhizal species had higher soil bacterial richness and symbiont types had distinct soil microbial community composition. Woody plant leaf traits indirectly influenced soil microbes through their impact on soil abiotic conditions, primarily soil pH and C:N ratios. Finally, site-level climate affected the overall magnitude and direction of woody plant influence, as soil fungal and bacterial richness were either higher or lower in woody encroached versus herbaceous soils depending on mean annual temperature and precipitation. All together, these results document global impacts of woody plant encroachment on soil microbial communities, but highlight that multiple biotic and abiotic pathways must be considered to scale up globally from site- and species-level patterns. Considering both the aboveground and belowground effects of woody encroachment will be critical to predict future changes in alpine ecosystem structure and function and subsequent feedbacks to the global climate system.


Assuntos
Ecossistema , Solo , Clima , Nitrogênio/análise , Plantas
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