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Environ Microbiol ; 26(2): e16584, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38372423


Coastal bays, such as Delaware Bay, are highly productive, ecologically important transitions between rivers and the coastal ocean. They offer opportunities to investigate archaeal assemblages across seasons, with the exchange of water masses that occurs with tidal cycles, and in the context of variable organic matter quality. For a year-long estuarine, size-fractionated time series, we used amplicon sequencing, chemical measurements, and qPCR to follow archaeal groups through the seasons. We detected seasonally high abundances of Marine Group II archaea in summer months which correlate with indicators of phytoplankton production, although not phytoplankton biomass. Although previous studies have reported associations between Marine Group II archaea and particles, here they are almost entirely found in very small particles (0.22-0.7 µm), suggesting they are free-living cells. Populations of Nitrososphaeria did not vary with particle size or environmental conditions. Methanogens were significant fractions of archaeal sequences in large particles at low tide during winter months. Contrary to expectations, Nanoarchaeia were found predominantly in the free-living fraction despite the previous observation that they require an association with hosts. These results underscore the utility of time series studies in shallow, tidally mixed estuarine environments that capture variable conditions for understanding the ecology and biogeochemistry of planktic archaea.

Archaea , Ecologia , Archaea/genética , Fatores de Tempo , Fitoplâncton/genética , Rios , Estações do Ano
Microbiologyopen ; 10(4): e1228, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34459548


The permanently stratified water columns in euxinic meromictic lakes produce niche environments for phototrophic sulfur oxidizers and diverse sulfur metabolisms. While Green Lake (Fayetteville, New York, NY) is known to host a diverse community of ecologically important sulfur bacteria, analyses of its microbial communities, to date, have been largely based on pigment analysis and smaller datasets from Sanger sequencing techniques. Here, we present the results of next-generation sequencing of the eubacterial community in the context of the water column geochemistry. We observed abundant purple and green sulfur bacteria, as well as anoxygenic photosynthesis-capable cyanobacteria within the upper monimolimnion. Amidst the phototrophs, we found other sulfur-cycling bacteria including sulfur disproportionators and chemotrophic sulfur oxidizers, further detailing our understanding of the sulfur cycle and microbial ecology of euxinic, meromictic lakes.

Chlorobi/isolamento & purificação , Chromatiaceae/isolamento & purificação , Cianobactérias/isolamento & purificação , Lagos/microbiologia , Enxofre/metabolismo , Chlorobi/classificação , Chlorobi/genética , Chromatiaceae/classificação , Chromatiaceae/genética , Cianobactérias/classificação , Cianobactérias/genética , Sequenciamento de Nucleotídeos em Larga Escala , Microbiota/genética , New York , RNA Ribossômico 16S/genética , Microbiologia da Água