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1.
Fitoterapia ; 165: 105426, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36608710

RESUMO

Hydrogen bond effect plays a pivotal role in protein-ligand interaction and represents one of the fundamental bases in pharmaceutical design. To evaluate the influence of hydrogen bond interaction on the anti-breast cancer activity, fifteen dihydroartemisinin-isatin hybrids 7a-o with hydrogen bond donors at C-3 position of isatin moiety were designed, synthesized and evaluated for their antiproliferative activity against MCF-7, MDA-MB-231, MCF-7/ADR and MDA-MB-231/ADR breast cancer cell lines. The preliminary results illustrated that introduction of hydrogen bond donors especially thiosemicarbazide into C-3 position of isatin moiety was beneficial for the activity, and substituents at C-5 position of isatin fragment as well as the length of the carbon spacers between dihydroartemisinin and isatin moieties also have significant influence on the activity. The enriched structure-activity relationships may provide useful information for further rational design of the candidates with higher activity.

2.
PLoS One ; 18(1): e0275903, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36701341

RESUMO

Empathy involves both empathic ability and empathic motivation. An important topic has been how to measure empathic ability and motivation simultaneously in both clinical and non-clinical samples and across different cultures. The Empathy Components Questionnaire (ECQ) is a self-report questionnaire that measures empathic ability and motivation in a questionnaire. The current study aimed to validate the Mandarin Chinese version of the ECQ (ECQ-Chinese) in three Chinese samples. In study 1, a total of 538 Chinese participants (Sample 1) completed the ECQ-Chinese via an online survey, and existing measures of empathy and related constructs which were used for criterion validity. In study 2, a total of 104 participants (Sample 2) were recruited again from sample 1 and completed the ECQ-Chinese three weeks later to investigate test-retest reliability. In study 3, a further 324 participants (Sample 3) completed the ECQ-Chinese for confirmatory factor analysis. The results showed that the ECQ-Chinese has a good internal consistency reliability, split-half reliability, and criterion validity (Study 1), and a good test-retest reliability (Study 2). Further, Study 3 found that a 22-item ECQ-Chinese consisting of five subscales had a good construct validity, convergence validity and discriminate validity, demonstrating it to be a suitable tool for the measurement of empathic ability and motivation in Chinese samples and to carry out cross-cultural studies of empathy and its components.

3.
Pest Manag Sci ; 2023 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-36640042

RESUMO

BACKGROUND: Insect-associated Streptomyces is a valuable resource for development of compounds with antibacterial potential. However, relatively little is known of the secondary metabolites produced by termite-associated Streptomyces. RESULTS: Here, seven compounds including o-acetaminophenol (1), phenazine-1,6-dicarboxylic acid (2), phenylacetic acid (3), phenazinolin D (4), izumiphenazine A (5), izumiphenazine B (6) and phenazinolin E (7) were obtained from the fermentation broth of a termite-associated Streptomyces showdoensis BYF17, which was isolated from the body surfaces of Odontotermes formosanus. Two additional novel derivative compounds (6a and 6b) were synthesized via acetylation and methylation, respectively. The structures of these compounds were elucidated by spectroscopic analyses. The antibacterial bioassay showed that compound 6a displayed strong inhibitory effects against Pseudomonas syringae pv. actinidiae (Psa), with a zone of inhibition (ZOI) diameter of 20.6 mm, which was comparable to that of positive gentamicin sulfate with a ZOI value of 25.6 mm. Furthermore, the Day 5 curative activities of both compounds 6 and 6a against kiwifruit bacterial canker were 71.5%, which was higher than those of referred oxine-copper (55.0%) and ethylicin (46.8%) at a concentration of 200 µg mL-1 . In addition, the mechanism analysis based on scanning electron microscopic observation revealed that both compounds 6 and 6a destroyed the integrity of the Psa cell membrane. CONCLUSION: The results of biological tests showed that these bioactive compounds exhibit potent antimicrobial activities, which have the potential to be developed into new antibacterial agents. © 2023 Society of Chemical Industry.

4.
Biology (Basel) ; 12(1)2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36671800

RESUMO

The Chinese razor clam (Sinonovacula constricta) is an important for Chinese aquaculture marine bivalve that naturally occurs across intertidal and estuarine areas subjected to significant changes in salinity level. However, the information on the molecular mechanisms related to high salinity stress in the species remain limited. In this study, nine gill samples of S. constricta treated with 20, 30, and 40 ppt salinity for 24 h were used for whole-transcriptome RNA sequencing, and a regulatory network of competing endogenous RNAs (ceRNAs) was constructed to better understand the mechanisms responsible for adaptation of the species to high salinity. A total of 83,262 lncRNAs, 52,422 mRNAs, 2890 circRNAs, and 498 miRNAs were identified, and 4175 of them displayed differential expression pattern among the three groups examined. The KEGG analyses of differentially expressed RNAs evidenced that amino acid synthesis and membrane transport were the dominant factors involved in the adaptation of the Chinese razor clam to acute salinity increase, while lipid metabolism and signaling played only a supporting role. In addition, lncRNA/circRNA-miRNA-mRNA regulatory networks (ceRNA network) showed clearly regulatory relationships among different RNAs. Moreover, the expression of four candidate genes, including tyrosine aminotransferase (TAT), hyaluronidase 4 (HYAL4), cysteine sulfinic acid decarboxylase (CSAD), and ∆1-pyrroline-5-carboxylate synthase (P5CS) at different challenge time were detected by qRT-PCR. The expression trend of TAT and HYAL4 was consistent with that of the ceRNA network, supporting the reliability of established network. The expression of TAT, CSAD, and P5CS were upregulated in response to increased salinity. This might be associated with increased amino acid synthesis rate, which seems to play an essential role in adaptation of the species to high salinity stress. In contrast, the expression level of HYAL4 gene decreased in response to elevated salinity level, which is associated with reduction Hyaluronan hydrolysis to help maintain water in the cell. Our findings provide a very rich reference for understanding the important role of ncRNAs in the salinity adaptation of shellfish. Moreover, the acquired information may be useful for optimization of the artificial breeding of the Chinese razor clam under aquaculture conditions.

5.
Materials (Basel) ; 16(2)2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36676508

RESUMO

The dynamic modulus is a key property determining the short- and long-term performance of asphalt pavement, and its strong dependence on confining pressure and material density (mixture compactness) has been clearly indicated in the literature. It is always challenging to reproduce three-dimensional in situ stress conditions in the laboratory. To alleviate this difficulty, in this study, a convenient experimental setup was developed, in which the lateral confinement was made present and variable as a concomitant reaction of the surrounding materials to the vertical loading. Three dense-graded mixtures were prepared to a set of four different densities and then subjected to the confined dynamic modulus test. The results indicated a significant dependence of the confined modulus on the three factors of temperature, frequency, and compactness and that the mixture with coarser gradation demonstrated a less sensitivity to these parameters. A mathematical model was developed for the dynamic modulus master curve unifying these factors by means of horizontal shifting due to the time-temperature superposition principle (validated against the variable confinement at different compactness) and the vertical shift factor as a function of reduced frequency and compactness. The adequacy of the model was demonstrated using the experimental data, and its potential application in field pavement compaction was discussed.

6.
Oral Dis ; 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36597156

RESUMO

BACKGROUND: Head and neck dermatofibrosarcoma protuberans (HNDFSP) is extremely rare and not entirely understood. OBJECTIVE: To investigate the clinicopathological features of HNDFSP and identify the expression of its clinically relevant indicators, with the expectation of improving the existing treatment strategies. METHODS: A long-term follow-up of patients with HNDFSP who received treatment between 2000 and 2021 at Shanghai Ninth People's Hospital was conducted. The clinical, histological, and immunohistochemical data of the patients were retrieved and analyzed. The endpoint of the study was the incidence of significant disease-related clinical events (recurrences or metastasis). RESULTS: A total of 49 patients with HNDFSP were included in the study, with males (92.7%) predominating than females (7.3%). Eighteen patients developed recurrent disease (36.8%) after surgery, and the median time of recurrence was 48 months (interquartile, 20-74 months). Metastasis occurred in two cases (4.1%). Two patients died during follow-up, both with local recurrence, and one of them with intestinal metastasis. Post-operation radiotherapy was administered to eight patients (16.3%) and the effect in local control was remarkable. Age, tumor size, and negative margins with sufficient safety width were the main independent factors affecting the disease-free survival. Several potential targeted therapeutic indicators, including EZH2 (80.0%), EGFR (91.4%), PDGF (97.1%), PD-L1 (77.1%), and VEGF (77.1%), were positively expressed in most tumor samples. CONCLUSION: HNDFSP is rare, significantly challenging to control locally, and has a worse prognosis with current treatment strategies. Wide local excision and long-term follow-up are needed. Radiotherapy could improve the prognosis of patients with HNDFSP.

7.
Opt Lett ; 48(2): 371-374, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36638460

RESUMO

The spin Hall effect (SHE) of light has brought important applications, but the involved spin states only split in one direction. Here we employ an accurate three-dimensional model of light to show that the SHE generally exhibits quadratic spin splitting, i.e., both vertical and horizontal splitting, in the presence of a fast phase change of reflection. Further, we disclose that the two splittings are actually different from each other, and that they originate from the vertical and horizontal spin momentum flows, respectively, owing to the spatial gradient of polarization in the individual direction. Finally, it is found that by tuning the incident angle and polarization of light, one can manipulate the quadratic SHE so as to realize a variety of spin splittings, such as unbalanced quadratic splitting and off-center splitting of spin states.

8.
ACS Appl Mater Interfaces ; 15(2): 3131-3140, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36603144

RESUMO

Flexible sensors have attracted increasing attention owing to their important applications in human activity monitoring, medical diagnosis, and human-machine interaction. However, the rational design of low-cost sensors with desirable properties (e.g., high sensitivity and excellent stability) and extended applications is still a great challenge. Herein, a simple and cost-effective strategy is reported by immersing polyurethane (PU) sponge in graphene oxide solution followed by in situ chemical reduction to construct a reduced graphene oxide (RGO)-wrapped PU sponge sensor. Ascribed to the excellent compressive resilience of PU sponge and an electrically conductive RGO layer, the constructed flexible sensor exhibits satisfactory sensing performance with high sensitivity (17.65 kPa-1) in a low-load range (0-3.2 kPa), a wide compression strain range (0-80%), and reliable stability (8000 cycles). In addition, these sensors can be successfully applied to monitor human movements and identify the weight of objects. Through the use of a sensor array integrated with a signal acquisition circuit, the reasonably designed sensors can realize tactile feedback via mapping real-time spatial distribution of pressure in complicated tasks and show potential applications in flexible electronic pianos, electronic skin, and remote real-time control of home electronics.


Assuntos
Grafite , Dispositivos Eletrônicos Vestíveis , Humanos , Movimento (Física) , Grafite/química , Movimento , Poliuretanos/química
9.
Bioorg Chem ; 131: 106139, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36610251

RESUMO

O-GlcNAcylation is a ubiquitous post-translational modification governing vital biological processes in cancer, diabetes and neurodegeneration. Metabolic chemical reporters (MCRs) containing bio-orthogonal groups such as azido or alkyne, are widely used for labeling of interested proteins. However, most MCRs developed for O-GlcNAc modification are not specific and always lead to unexpected side reactions termed S-glyco-modification. Here, we attempt to develop a new MCR of Ac34FGlcNAz that replacing the 4-OH of Ac4GlcNAz with fluorine, which is supposed to abolish the epimerization of GALE and enhance the selectivity. The discoveries demonstrate that Ac34FGlcNAz is a powerful MCR for O-GlcNAcylation with high efficiency and the process of this labeling is conducted by the two enzymes of OGT and OGA. Most importantly, Ac34FGlcNAz is predominantly incorporated intracellular proteins in the form of O-linkage and leads to negligible S-glyco-modification, indicating it is a selective MCR for O-GlcNAcylation. Therefore, we reason that Ac34FGlcNAz developed here is a well characterized MCR of O-GlcNAcylation, which provides more choice for label and enrichment of O-GlcNAc associated proteins.


Assuntos
Processamento de Proteína Pós-Traducional , Proteínas , Proteínas/química , Acetilglucosamina/química
10.
Ren Fail ; 45(1): 2159431, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36632821

RESUMO

BACKGROUND: Diabetic kidney disease (DKD) is a leading cause of kidney failure worldwide. Anxiety has been associated with disease progression in non-diabetes patients. We aimed to examine the prospective association between anxiety and progression of DKD in type 2 diabetes. METHODS: We conducted a prospective cohort study of 2040 participants with type 2 diabetes at the Diabetes Center of Shanghai General Hospital between May 2017 and June 2020. Anxiety disorders at baseline were diagnosed by a structured clinical interview based on the 10th Revision of International Classification of Disease (ICD). Progression of DKD was identified as the transition from one urinary albumin excretion rate (AER) stage to the next or the development of kidney failure during the follow-up period. RESULTS: At baseline, 403 (19.8%) had a diagnosis of anxiety disorders, of whom 107 (26.6%) also received a depression diagnosis. During a median follow-up time of 3.2 years, deterioration of the kidney status occurred in 340 (16.7%) individuals. After adjustment for potential confounders including depression or an anxiety × depression interaction term, anxiety disorders were independently related to an increased risk of progression of DKD (HR 1.539, 95% CI 1.130-2.095, p = 0.006; HR 1.536, 95% CI 1.111-2.122, p = 0.009, respectively). CONCLUSIONS: Anxiety disorders at baseline, independent of possible confounders, were associated with the progression of DKD in type 2 diabetes. Whether therapeutic interventions for anxiety reduce the risk needs to be investigated.


Assuntos
Diabetes Mellitus Tipo 2 , Nefropatias Diabéticas , Insuficiência Renal , Humanos , Nefropatias Diabéticas/complicações , Diabetes Mellitus Tipo 2/complicações , Estudos Prospectivos , Progressão da Doença , China/epidemiologia , Insuficiência Renal/complicações , Transtornos de Ansiedade/etiologia , Transtornos de Ansiedade/complicações
11.
J Stroke Cerebrovasc Dis ; 32(2): 106946, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36527944

RESUMO

PURPOSE: To clarify the safety and efficacy of "X" stent-assisted coiling (X-stenting) for the treatment of intracranial bifurcation aneurysms and to review the literature. METHODS: We retrospectively reported five consecutive patients with six intracranial bifurcation aneurysms who underwent X-stenting between June 2014 and June 2020. The clinical and angiographic results were analyzed. We also performed an extensive PubMed review of medical literature up to June 2021. RESULTS: Endovascular procedures were successfully applied to all six aneurysms. No procedure-related complications were noted. Follow-up angiograms were available for all patients and revealed occlusion in five aneurysms and improvement in one aneurysm. Clinical follow-up was performed for all patients, and the mRS score at follow-up was 0 in all patients. Thus, good outcomes were achieved in all patients, and no ischemic or hemorrhagic events were observed. CONCLUSIONS: X-stenting appears to be safe for treating intracranial bifurcation aneurysms and effective in immediate and short-term outcome. Further research is required with well-designed, prospective studies with large sample sizes.


Assuntos
Embolização Terapêutica , Procedimentos Endovasculares , Aneurisma Intracraniano , Humanos , Resultado do Tratamento , Estudos Retrospectivos , Estudos Prospectivos , Angiografia Cerebral/métodos , Stents , Aneurisma Intracraniano/terapia , Aneurisma Intracraniano/tratamento farmacológico , Embolização Terapêutica/efeitos adversos , Embolização Terapêutica/métodos , Procedimentos Endovasculares/efeitos adversos , Procedimentos Endovasculares/métodos
12.
ACS Nano ; 17(1): 725-734, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36575649

RESUMO

Plasmonic surface lattice resonances (SLRs) supported by metallic nanoparticle (NP) arrays show diverse applications including nanolasers, sensors, photocatalysis, and nonlinear optics. However, to rationally fabricate high-quality plasmonic NP arrays with ultranarrow SLR line widths over large areas remains challenging. This article describes a general approach for the efficient fabrication of centimeter-scale inorganic NP arrays with precisely controlled NP size, composition, position, and lattice geometry. This method combines the processes of solvent-assisted soft lithography and in situ site-specific NP growth to reproducibly create many replicates of NP arrays without utilizing cleanroom and specialized equipment. For demonstration, we show that Au NP arrays exhibit ultranarrow SLRs with a line width of 4 nm and a quality factor of 218 toward the theoretical limit.

13.
J Ethnopharmacol ; 303: 116053, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36529247

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Liver fibrosis is a potentially harmful chronic liver disease caused by various etiologies. There is currently no specific drug for liver fibrosis. Xiaochaihu Tang (XCHT) is a traditional formula combined of seven herbs, which was first recorded in the Treatise on Febrile Diseases in Han Dynasty of ancient China. It is widely used in clinic to hepatic protection, analgesic, antipyretic and anti-inflammatory treatment. And it has been recommended for treating chronic hepatitis and chronic cholecystitis in the latest guidelines for the diagnosis and treatment of liver fibrosis with integrated traditional and western medicine. However, the underlying regulatory mechanisms remain elusive. AIM OF THE STUDY: This study aims to explore the therapeutic effects of XCHT on liver fibrosis and its underlying molecular mechanisms from the perspective of network pharmacology and experimental research. MATERIALS AND METHODS: Carbon tetrachloride (CCl4) induced and bile duct ligation (BDL) induced liver fibrosis models in mice were established to evaluate the anti-fibrosis effects of XCHT in vivo. Potential anti-fibrosis targets of XCHT were screened via network establishment. The underlying mechanisms were uncovered through GO and pathway enrichment analysis. Then, the core targets were identified from protein-protein interaction network by means of the Cytohubba plug-in of Cytoscape. Furthermore, two effective monomer components of XCHT were recognized by molecular docking. Moreover, the predicted components and pathways were verified by in vitro experiments. RESULTS: When treated with XCHT, liver fibrosis was alleviated in both mice models, showing as the improvement of liver function, the protection of hepatocytes, the inhibition of HSC activation and the reduction of hepatic collagen accumulation. 540 monomer components, 300 therapeutic targets, 109 signaling pathways, 246 GO biological processes, 77 GO cellular components, 107 GO molecular functions items and core targets were identified by network analysis. Then, 6-gingerol and baicalein were identified as the core components of anti-fibrosis effects of XCHT via leptin or Nrf2 signaling pathway. Furthermore, the experiment in vitro also validated the results. CONCLUSIONS: Our study suggests XCHT could alleviate liver fibrosis through multi-targets and multi-pathways; 6-gingerol and baicalein are its core components which may play an important role via leptin or Nrf2 signaling pathway.


Assuntos
Medicamentos de Ervas Chinesas , Leptina , Animais , Camundongos , Simulação de Acoplamento Molecular , Farmacologia em Rede , Fator 2 Relacionado a NF-E2 , Cirrose Hepática/tratamento farmacológico , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico
14.
Int Immunopharmacol ; 114: 109531, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36513023

RESUMO

To investigate the characteristics and functions of yak ß-defensin 124 (DEFB124), prokaryotic expression, analysis of gut microbiological and other methods were used in this study. The results showed that the sequence of yak DEFB124 gene was 306 bp in length and 207 bp in open reading frame, which encoded 68 amino acids. Yak DEFB124 protein was highly conserved and had the closest relationship with cattle. Yak DEFB124 protein was a secreted cationic ß-defensin. The recombinant expression plasmid pET32a-DEFB124 was constructed, and an about 24 kDa protein was successfully expressed. Yak DEFB124 protein had inhibitory activity against Staphylococcus aureus (S. aureus), and its MIC value was 64 µg/mL. After treating with yak DEFB124 protein, the activities of alkaline phosphatase (AKP) and total superoxide dismutase (T-SOD) were higher (P < 0.01) in the jejunum tissue, but the activity of lysozyme (LZM) was lower (P < 0.01). The number of goblet cells in the duodenum, jejunum, and ileum of the mice in the DEFB124 group was increased (P < 0.01). Besides, the expressions of MUC2 mRNA and protein were increased (P < 0.05) after the treatment with yak DEFB124 protein. Furthermore, the relative abundance of Lactobacillus in jejunum of mice in DEFB124 group was also increased. In summary, yak DEFB124 protein could increase the number of goblet cells in mice intestine and the abundance of intestinal probiotics Lactobacillus, thereby protecting the intestinal tract and alleviating intestinal damage.


Assuntos
Infecções Estafilocócicas , beta-Defensinas , Animais , Bovinos , Camundongos , beta-Defensinas/genética , beta-Defensinas/imunologia , Células Caliciformes , Probióticos , Infecções Estafilocócicas/imunologia , Staphylococcus aureus , Intestinos/imunologia , Intestinos/patologia
15.
Dalton Trans ; 52(2): 434-443, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36524392

RESUMO

In this article, a novel triphenylamine-modified salicylaldehyde Schiff base 2-(((4-(diphenylamino)phenyl)imino)methyl)-4-(pyridine-4-yl)phenol (HL) was synthesized and structurally characterized. HL possessed D-π-A structure and exhibited typical AIE property in THF/H2O. It was applied to selectively recognize Cu2+ through an on-off mode in THF/H2O (1/9, v/v), and the fluorescence attenuation was attributed to a paramagnetic quenching effect of Cu2+ together with the abatement of HL aggregates. Hence, the detection limit achieved was as low as 1.32 × 10-7 M. The spectroscopic and ESI-HRMS results revealed a 1 : 2 complexation ratio of Cu2+ with HL. The mechanism for sensing Cu2+ was further confirmed by performing DFT calculations. Owing to the large affinity between Cu2+ and His, the resultant CuL2 system was further used to detect His via the off-on method based on the displacement of ligands. The detection limit for His reached 5.14 × 10-8 M. Furthermore, HL was available to prepare handy indicator papers for the on-site recognition of Cu2+ and His. Confocal fluorescent imaging demonstrated that HL could sequentially respond to intracellular Cu2+ and His.


Assuntos
Cobre , Bases de Schiff , Bases de Schiff/química , Espectrometria de Fluorescência , Cobre/química
16.
Sci Total Environ ; 863: 160712, 2023 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-36509282

RESUMO

The contributions of urban blue and green spaces on urban cooling are widely acknowledged. However, the combined cooling effect of integrated blue and green space remains uncertain. In this study, a combination of modelling and observational analyses uncovered a win-win interaction between coexisting blue and green spaces in terms of urban cooling. That is, the integration of water bodies and green spaces can reinforce the mutual cooling potential and provide greater urban cooling than stand-alone water bodies and green spaces. The results indicated that the known influencing factors such as area, shape and planting structure had no impact on the cooling effect of riverside urban green spaces. Instead, the width of the adjacent river reach and the degree of contact with the reach were significantly positively related to the cooling effect of riverside green spaces. The surface/air temperature of a riverside green space can be 4.2 °C/3.7 °C lower in the daytime in summer, and 3.1 °C/2.7 °C lower in spring than a non-riverside green space of similar size. Urban green spaces with water bodies inside could cause about 0.99 °C and 1.45 °C more deduction of land surface temperature (LST) than simple green spaces of similar size in spring and summer, respectively. There were about 1 °C­2.9 °C more deductions in the air temperature of a river reach when it is accompanied by green spaces. More specifically, complete coverage with vegetated areas within a 30 m buffer on both riverbanks can result in a 3.1 °C and 3.37 °C higher LST deduction compared to no vegetation coverage on the riverbank in the daytime in spring and summer, respectively. The results of this study extend the understanding of the cooling effect of urban blue-green spaces and provide implications for sustainable urban planning.

17.
Anal Chem ; 95(2): 1287-1293, 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36535709

RESUMO

The elimination of aggregation-caused quenching of polycyclic aromatic hydrocarbons by metal-ligand coordination is of immense scientific interest in solid-state electrochemiluminescence (ECL) sensing. Herein potassium ion (K+)-mediated J-aggregate K-PTC MOF (PTCA, perylene-3,4,9,10-tetracarboxylic) was synthesized and employed to formulate an ECL immunosensor for biomarker detection. The coordination-driven aggregates are arranged in an end-to-end side mode, which overcomes the aggregation-caused quenching related to PTCA concentration. Compared with PTCA, K-PTC MOF shows a more stable ECL emission with an unprecedented red shift to 718 nm and is equipped with ECL activity for analytical applications at a voltage of -1.1 V. Considering the requirements of accurate detection, metal-phenolic bioactive nanoparticles (MPNPs) were synthesized for the construction of a sandwich sensing platform to realize the steady-state regulation of ECL. As proof of applicability, a constructive experiment was carried out with neuron-specific enolase (NSE), a marker of small cell lung cancer (SCLC), as a targeted analyte. With optimal requirements, the configuration can provide a detection range of 10 pg/mL to 50 ng/mL and a detection limit of 7.4 pg/mL, accompanied by sufficient practical analytical performance. Collectively, this paradigm provides a deeper understanding of the ECL characteristics of coordination-driven J-aggregation and provides more possibilities for the development of ECL patterns based on luminescent metal-organic frameworks.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Medições Luminescentes , Técnicas Eletroquímicas , Imunoensaio , Potássio , Biomarcadores , Limite de Detecção
19.
J Environ Manage ; 328: 116947, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36508977

RESUMO

Large-scale ecological restoration programs have been initiated globally with the aim of combating desertification and improving ecosystem services, especially for sand fixation service (SF) in arid and semi-arid regions. However, the effectiveness of ecological restoration in the radiation benefit of SF, such as improving air quality, remains not well known. In this study, we selected Xilingol as the study area, investigated the dynamics of SF, and quantified the radiation benefit of SF in downwind areas by employing PM10 concentration as the proxy. The Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model was applied to assess the response of radiation benefit to ecological restoration by designing land use scenarios. Results showed that the SF in Xilingol increased with fluctuation at an average rate of 0.27%/year from 2000 to 2018. Under the effect of ecological restoration, the radiation benefit in the downwind regions was substantially improved, as manifested by a 104.22 µg/m3 reduction in PM10 concentration. The changes in radiation benefit varied greatly across space, and northern and southern Xilingol were hot spots for increased radiation benefit. Based on regional disparity in benefit level, this work could provide a reference to make differentiated cross-regional ecological compensation schemes at the national level.


Assuntos
Ecossistema , Areia , China , Clima Desértico , Conservação dos Recursos Naturais
20.
Food Chem ; 405(Pt B): 134972, 2023 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-36423554

RESUMO

Honey crystallization is a common occurrence, while the underlying mechanisms are not clear. Here we evaluated natural crystallization and induced crystallization of honey. By using the method of microscopy, colorimetry, rheology, texture, and calorimetry to investigate the main physical and structural properties and compare with honey seed crystals-induced crystallization in six unifloral honey. The results showed honey transformed into a pseudoplastic fluid during natural crystallization, with the increase of L-value, firmness and cohesiveness and the fastest crystallization rate occurring at 14 °C and water content of 16%. The effect of the addition of seed crystals in the honey crystallization can be summarized as the acceleration of crystallization rate (3 times) and the improvement of crystallization characteristics including uniform particles and reducing stratification. This study provides insight into honey crystallization theory and a resource for the further exploration and development of the new performance of honey products.


Assuntos
Mel , Calorimetria , Cristalização , Reologia
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