Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.197
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Food Chem ; : 125656, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31699558

RESUMO

The adulteration of honey is generally a safety and quality concern for consumers and the industry as a whole. Resin technologies allow harmful substances to enter honey, creating substandard honey, which can enter the market. Thus, it is necessary to identify such illegal products quickly and easily. In this study, HPLC-ECD combined with chemometrics was used to identify raw acacia honey that had been treated with macroporous adsorption resins. The chromatography fingerprints of 46 honey samples were established, and principal component analysis (PCA) and the OPLS-DA identified that differences in some of the chromatographic peaks could be used to distinguish raw from resins-treated raw honeys. 100% correct classification was achieved with test samples, based on the chromatographic peaks identified. These results show that HPLC-ECD, combined with chemometric methods, can identify correctly resins-treated honey and can be applied for the quality control of honey.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31703999

RESUMO

In this study, a novel raw material, Bermuda grass had been devised for the synthesis of activated carbon (BGAC) and enhanced the pore volume by potassium hydroxide. The effects of different factors on activated carbon products by orthogonal experiment was optimized. The synthesized BGAC was characterized by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) analysis, energy dispersive X-ray (EDX), X-ray photoelectron spectroscopy (XPS) and then, Cr(VI) removal batch experiments were conducted to investigate the Cr(VI) removal performance. Kinetic model and Weber-Morris diffusion model were fitted to the Cr(VI) removal process indicated that the chemisorption was the predominant removal mechanism and intraparticle diffusion was the sole rate-controlling mechanism. Langmuir isotherms could fit the experimental date well, which revealed that the adsorption of Cr(VI) ions was monolayer adsorption and the maximum adsorption capacity could be reached at 403.23 mg g-1. The results also promulgated that BGAC had an excellent potential on Cr(VI) removal. The removal processes were considered to comprise adsorption, reduction, precipitation and other ways through the study of the removal mechanism.

3.
Clin Respir J ; 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31758867

RESUMO

INTRODUCTION: The characteristics of Allergic Bronchopulmonary Aspergillosis (ABPA) based on its radiological classification is still unclear. OBJECTIVES: To investigate the clinical significances of ABPA patients with central bronchiectasis (ABPA-CB) by different radiological classifications of mucus plugs. METHODS: ABPA-CB patients from a pulmonary hospital between 2008 to 2015 were retrospectively included and analyzed. According to the chest imaging in their first visit to physician, the ABPA-CB patients were divided into two groups based on the presence of high-attenuation mucus (HAM) or low-attenuation mucus (LAM). The primary endpoint was ABPA relapse within 1 year since the glucocorticoid withdrawal. The relationship between the imaging findings and clinical prognosis was illuminated. RESULTS: A total of 125 ABPA patients were analyzed in this study. Compared to the LAM group, the HAM group presented higher blood eosinophil cells counts, higer rates of Aspergillus detection isolated in sputum and expectoration of brownish-black mucus plugs, more affected lobes and segments, poorer pulmonary function and higher rate of relapse. CONCLUSIONS: The clinical characteristics and prognosis of ABPA-CB patients are closely related to its radiological phenotype of mucus plugs in the central bronchiectasis. Clinicians should promote a diversity of personalized treatments for different patients with different radiological characteristics.

4.
Mikrochim Acta ; 186(11): 743, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31686218

RESUMO

This work describes the synthesis of a nanocomposite consisting of Ag2O, silver nanoparticles and N,S-doped carbon quantum dots (Ag2O/Ag@NS-CQD). The NS-CQD were prepared by hydrothermal treatment of p-aminobenzenesulfonic acid. They act as both the reducing and stabilizing agent for synthesis of Ag2O/Ag@NS-CQD. The composite was characterized by UV-vis spectroscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The glassy carbon electrode (GCE) was modified by coating it with Ag2O/Ag@NS-CQD. It exhibits excellent amperometric response to catechol, typically at a low working potential of around 0.25 V. Under the best experimental conditions, the sensor has a wide linear response (0.2 to 180 µM) and a low detection limit (13 nM; at S/N = 3). The method was applied to analysis of spiked water samples and gave satisfactory results. Graphical abstract Schematic representation of the preparation of the Ag/Ag2O@N,S-doped carbon quantum dots composite using p-aminobenzenesulfonic acid and silver nitrate as the starting materials. The corresponding modified glassy carbon electrode exhibits the excellent amperometric sensing performance toward catechol at pH 7.0 with low detection limit and good selectivity.

5.
Mikrochim Acta ; 186(12): 811, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31745662

RESUMO

The N-terminal pro-B-type natriuretic peptide (NT-proBNP) is a marker of heart failure. A novel sandwich type electrochemiluminescence (ECL) immunoassay is described for the NT-proBNP. The method is based on ECL resonance energy transfer (RET) between silver nanocubes that were covered with semicarbazide-modified gold nanoparticles (AgNC-sem@AuNPs) as the donor, and a Ti(IV)-based metal-organic framework of type MIL-125 as the acceptor. The ECL signal was strongly amplified by increasing the luminous efficiency. ECL-RET occurs due to the partial overlap between the ECL emission of the AgNC-sem@AuNPs (emission wavelength at 470 nm to 900 nm) and the visible absorption spectrum of MIL-125 (absorption wavelength at 406 nm to 900 nm). This results in the quenching of ECL. The AgNC-sem@AuNPs were placed on the electrode. The antibody was immobilized on AgNC-sem@AuNPs via Au-NH2 bond, and MIL-125 was utilized as a label for the secondary antibody. The assay works in the 0.25 pg mL-1 to 100 ng mL-1 concentration range and has a 0.11 pg mL-1 lower detection limit (at S/N = 3). Graphical abstract Schematic representation of self-enhanced luminescence mechanism (semicarbazide (Sem) as co-reaction accelerator) and Electrochemiluminescence resonance energy transfer (ECL-RET): silver nanocubes (AgNCs) as the energy donor and MIL-125 as the energy acceptor.

6.
Zootaxa ; 4590(2): zootaxa.4590.2.6, 2019 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-31716095

RESUMO

Epoicocladius Sulc et Zavrel from China is reviewed. Epoicocladius wangi Liu et Yan sp. n. is described and illustrated as male imago from China. The generic diagnosis is amended. Key to the known adult males of the genus and species distribution map worldwide are presented.


Assuntos
Chironomidae , Dípteros , Animais , China , Masculino
7.
J Craniofac Surg ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31725504

RESUMO

OBJECTIVES: To get and analyze the metric data of zygomatic region for the plasty of zygoma. MATERIALS AND METHODS: A total of 108 dry skulls in Chinese Han population were randomly collected and measured. The metrical data were divided into 4 parts, including the relative position of contour height of zygomatic bone, the relative prominence of zygomatic bone, the relative prominence of zygomatic arch, and the angle of the zygomatic bone and arch. RESULTS: The measurements in the 4 parts showed significantly difference between male and female (P < 0.05). For relative position of contour height of zygomatic bone group, the data of male is significantly bigger than female (P < 0.05). For relative prominence of zygomatic bone/zygomatic arch group, zygoma/zygomatic arch of male significantly protruded more than female (P < 0.05). CONCLUSION: The location of male zygoma is more protruding than female. The female zygoma is squarer than male and marginal process is helpful in zygomatic plasty. CLINICAL RELEVANCE: These studies show and analyze the metric data of zygomatic region in Chinese Han population for the plasty of zygoma. These different characters between males and females could be helpful in zygomatic plasty of Chinese Han population based on this research.

8.
J Phys Chem Lett ; 10(22): 7251-7258, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31698898

RESUMO

Water diffusion in nanopores has attracted considerable attention in the past decades. Recently the coupling between the vibration of pore walls and movement of confined water has been recognized to largely enhance diffusion. However, its impact on water diffusion in graphene oxide membranes remains to be discussed. Here we explore how water diffusion couples with the thermal fluctuation of graphene nanochannels by molecular dynamics simulations. Our finding demonstrates an approximately linear dependence of diffusion enhancement on temperature; i.e., the wiggling nanopore enhances diffusion at low temperature and inhibits diffusion at high temperature. This mechanism is further extended to be applicable for another two typical layered materials, hBN and MoS2. These results offer opportunities to tune surface diffusion by thermal operation or mechanical activation, advancing the application of two-dimensional materials in membrane separations.

9.
Org Lett ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31747294

RESUMO

Condition-controlled divergent oxidative coupling reactions between indole/tryptamine derivatives and ß-arylacrylic acids with the catalysis of copper(II) have been developed. Specifically, a formal Michael addition/dehydration sequence between indoles and ß-arylacrylic acids occurred in the presence of catalytic CuBr2 in CH3CN under air, thus affording highly functionalized 2,3-dihydro-1H-pyrrolo[1,2-a]indoles. In contrast, upon changing the oxidant to tBuOOH and the solvent to DCM, the reaction course switched to the unprecedented oxidative coupling/cyclization cascade to give the tetracyclic pyrrolo[2,3-b]indolines selectively.

10.
Nano Lett ; 19(11): 7735-7741, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31646868

RESUMO

Structural superlubricity, which promises an ultralow sliding friction due to the cancellation of the lateral force between two incommensurate interfaces, is a fundamental phenomenon in modern tribology. Achieving macroscale superlubricity is critical to its practical application, and the key is understanding how friction scales with real contact area, that is, the scaling law, especially for kinetic friction which accounts for most of the energy dissipation during sliding. Here, inspired by extensive molecular dynamics simulations we introduce an analytical general theory for the scaling law of structural superlubricity, which could well explain existing experimental measurements on the nanoscale. On the microscale, the scaling law is validated by measuring the friction of several microscale superlubric graphite/hexagonal boron nitride heterojunctions. The proposed theory predicts a characteristic size D = O(100 nm) above which the scaling transits from sublinear to linear. Our results provide insights in the origin of friction for structural superlubricity and benefit its application on macroscale.

11.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(5): 1387-1394, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31607288

RESUMO

OBJECTIVE: To investigate the effect of metformin on the proliferation, apoptosis and energy metabolism of acute myeloid leukemia (AML) K562 cells and the possible mechanism. METHODS: Different doses (0, 5, 10, 20 and 30 mmol/L) of metformin was added into the K562 cells, which were cultivated for 24 h, 48 h and 72 h. The inverted optical microscope was used to observe the cell growth, CCK 8 was used to detect the cell vitality. The appropriate metformin doses (0, 10, 20 and 30 mmol/L) and the best time (48 h) were selected for subsequent experiments. The flow cytometer with Annexin V-FITC /PI doulde staining was used to detect apoptosis; the glucose detection kit and lactate detection kit were used to detect glucose consumption and lactate production; fluorescence quantitative PCR was used to detect glycolysis-related gene expression, and Western blot was used to detect protein expression. RESULTS: Metformin inhibited the proliferation of K562 cells in a dose-dependent manner (r=0.92), and the relative survival in the 30 mmol/L group was as low as 19.84% at 72 h. When treated with metformin for 48 h, the apoptosis rates of 0, 10, 20 and 30 mmol/L groups were 5.14%, 12.19%, 26.29% and 35.5%, respectively. Compared with the control group, the glucose consumption and lactate secretion of K562 cells treated with metformin were significantly reduced (P<0.05), and showed a dose-dependent effect(r=0.94,r=0.93,respectively). Metformin inhibited the expression of GLUT1, LDHA, ALDOA, PDK1, and PGK1 genes of K562 cells (P<0.05) showing a dose-dependent manner(r=0.83,r=0.80,r=0.72,r=0.76,r=0.73,respectively). Metformin inhibited the expression of P-Akt, P-S6, GLUT1, LDHA proteins of K562 cells(P<0.05), showing a dose-dependent relationship(r=0.80,r=0.92,r=0.83,r=0.92,respectively). CONCLUSION: Metformin can inhibit the growth and proliferation of K562 cells and promote the apoptosis of K562 cells by inhibiting glycolysis energy metabolism. PI3K/Akt/mTOR signaling pathway may be one of the molecular mechanisms of metformin on k562 cells.


Assuntos
Metformina/farmacologia , Apoptose , Proliferação de Células , Glicólise , Humanos , Células K562 , Fosfatidilinositol 3-Quinases
12.
J Agric Food Chem ; 67(44): 12255-12263, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31618580

RESUMO

Jujube (Ziziphus jujuba Mill.) honey, one of the most valuable honey varieties from China with unique characteristics, is vulnerable to being the target of adulteration and deliberate mislabeling of botanical origin. This study investigated the typical protein component of jujube honey to authenticate the floral source by SDS-PAGE analysis combined with LC-MS/MS identification, and its stability to heating was also evaluated. One band and two adjacent but independent bands, both with molecular weights of ∼19 kDa, were notably observed in Coomassie brilliant blue- and silver-stained SDS-PAGE gels, respectively, for jujube honey from different geographic origins, whereas that was not present for the other five botanical honey varieties, suggesting this protein component was suitable as a marker for jujube honey. LC-MS/MS identification revealed that it was constituted by one Z. jujuba-derived protein (gene number:Zj.jz016003045) and two A. mellifera-derived proteins (an uncharacterized protein with accession number tr|A0A088AC16 and a cleavage fragment from major royal jelly protein-1), and the existence of plant-derived protein was attributed to the special neutral pH of jujube honey. Additionally, these protein markers exhibited good stability to heating below 85 °C/30 min. This study provided a simple method to characterize jujube honey and first identified a protein indicator to determine the botanical origin of honey.


Assuntos
Proteínas de Plantas/química , Ziziphus/química , Sequência de Aminoácidos , China , Cromatografia Líquida , Eletroforese em Gel de Poliacrilamida , Frutas/química , Frutas/genética , Mel/análise , Peso Molecular , Proteínas de Plantas/genética , Espectrometria de Massas em Tandem , Ziziphus/genética
13.
Anal Chim Acta ; 1089: 48-55, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31627818

RESUMO

Combining of amperometric and square wave voltammetric methods (SWV), the dual-signal sandwich electrochemical immunosensor was developed for quantitative determination of amyloid ß-protein (Aß). Cu was doped into Al2O3 lattice (Cu-Al2O3) and reacts with graphite carbon nitride (g-C3N4) to generate Cu-Al2O3-g-C3N4 with internal dual-reaction center structure, which has good catalytic properties of hydrogen peroxide (H2O2). Subsequently, palladium nanoparticles (Pd NPs) was introduced into Cu-Al2O3-g-C3N4 (Cu-Al2O3-g-C3N4-Pd) that not only synergistically catalyzed H2O2 but also immobilized anti-Aß (Ab1) via Pd-NH2. The Cu-Al2O3-g-C3N4-Pd was used as matrix material to modify the electrode, which can produce obviously electrochemical signals through Amperometry i-t curve. Meanwhile, the Zr6O4(OH)4(CO2)12 (UiO-66) modified with polyaniline (PANI) has the large specific surface, good conductivity and adsorption capacity, which can support methylene blue (MB) as signal label of anti-Aß (Ab2). Therefore, the UiO-66@PANI-MB can provide an obviously electrochemical signal about MB through SWV. Under optimal conditions, the dual-signal sandwich electrochemical immunosensor has salient analytical performance and both signal platforms provide more accurate results. The linear range of detection obtained by the immunosensor was 10 fg/mL-100 ng/mL, and the detection limit was 3.3 fg/mL. This method not only provided a reliable guarantee for the experimental detection but also provided an effective strategy for the detection of other biological.

14.
Medicine (Baltimore) ; 98(38): e17115, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31567949

RESUMO

BACKGROUND: The program aims to evaluate the effectiveness and safety of cupping in patients with chronic urticaria (CU). METHODS: We will search the databases including PubMed, Medline, Embase, the Cochrane Central Register of Controlled Trials (CENTRAL), Web of Science, China Science Journal Database, China National Knowledge Infrastructure, Wan-fang Database, and China Biomedical Literature Database from their inception to May 2019. In addition, we will manually search the list of medical journals as a supplement. The clinical randomized controlled trials or quasi-randomized controlled trials related to cupping for the treatment of CU will be included in the study. Data were synthesized by using a fixed-effect model or random effect model depend on the heterogeneity test. The primary outcome is the total effective rate. Secondary outcomes include skin disease quality of life index scores, adverse events, and recurrence rates. RevMan V.5.3 statistical software will be used for meta-analysis. If it is not appropriate for a meta-analysis, then a descriptive analysis will be conducted. Data synthesis will use the risk ratio and the standardized or weighted average difference of continuous data to represent the results. RESULTS: This study will provide a comprehensive review of the available evidence to assess the effectiveness and safety of cupping for patients with CU. CONCLUSION: This systematic review (SR) will provide evidence to judge whether cupping is an effective intervention for patients with CU. ETHICS AND DISSEMINATION: The protocol of the SR does not require ethical approval because it does not involve humans. We will publish this article in peer-reviewed journals and presented at relevant conferences. SYSTEMATIC REVIEW REGISTRATION: PROSPERO, CRD42019137451.


Assuntos
Terapia por Acupuntura , Urticária/terapia , Protocolos Clínicos , Humanos , Revisão Sistemática como Assunto
15.
Oxid Med Cell Longev ; 2019: 1301736, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31636802

RESUMO

The present study investigated the antiapoptotic and antigenotoxic capabilities of N-acetyl cysteine- (NAC-) containing polymethyl methacrylate (PMMA) resin. An in vitro Transwell insert model was used to mimic the clinical provisional restorations placed on vital teeth. Various parameters associated with cell apoptosis and genotoxicity were investigated to obtain a deeper insight into the underlying mechanisms. The exposure of human dental pulp cell (hDPC) cultures to the PMMA resin (Unifast Trad™) resulted in a rapid increase in reactive oxygen species (ROS) level beginning at 1 h, which was followed by time-dependent cell detachment and overt death. The formation of γ-H2AX and cell cycle G1 phase arrest indicated that oxidative DNA damage occurred as a result of the interactions between DNA bases and ROS, beyond the capacities of cellular redox regulation. Such oxidative DNA damage triggers the activation of p53 via the ataxia telangiectasia mutated (ATM) signaling pathway and the induction of intrinsic mitochondrial apoptosis. Oxidative stress, cell apoptosis, and DNA damage induced by the PMMA resin were recovered to almost the level of untreated controls by the incorporation of NAC. The results indicate that the PMMA resin induced the intrinsic mitochondrial apoptosis as a consequence of p53 activation via the ATM pathway in response to oxidative DNA damage. More importantly, the incorporation of NAC as a novel component into the Unifast Trad™ PMMA resin offers protective effects against cell apoptosis and genotoxicity. This procedure represents a beneficial strategy for developing more biocompatible PMMA-based resin materials.

16.
Artigo em Inglês | MEDLINE | ID: mdl-31647399

RESUMO

A novel Gram-stain-variable, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 7197T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Yakeshi County, Inner Mongolia, PR China. This isolate was found to have the highest 16S rRNA gene sequence similarity to Paenibacillussabinae T27T (98.0 %), followed by Paenibacillussophorae S27T (97.9 %) and Paenibacillusforsythiae T98T (97.7 %). To ascertain the genomic relatedness of this strain to its phylogenetic neighbours, its genome sequence was determined. The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. sabinae T27T, P. sophorae S27T and P. forsythiae T98T were 87.9 %, 85.8 and 83.9 %, respectively. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified aminolipid. The major cellular fatty acids were anteiso-C15 : 0 (56.3 %), C16 : 0 (15.7 %) and iso-C15 : 0 (14.1 %).The genome size of strain 7197T was 5.21 Mb, comprising 4879 predicted genes with a DNA G+C content of 51.9 mol%. Menaquinone-7 was reported as the major respiratory quinone. The diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid. Based on phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strain 7197T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillusrhizophilus sp. nov. is proposed. The type strain of Paenibacillusrhizophilus is 7197T (=DSM 103168T=CGMCC 1.15699T).

17.
Sci Adv ; 5(10): eaaw8417, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31663018

RESUMO

Although nuclear type 2C protein phosphatase (PP2Cδ) has been demonstrated to be pro-oncogenic with an important role in tumorigenesis, the underlying mechanisms that link aberrant PP2Cδ levels with cancer development remain elusive. Here, we found that aberrant PP2Cδ activity decreases p53 acetylation and its transcriptional activity and suppresses doxorubicin-induced cell apoptosis. Mechanistically, we show that BRCA1 facilitates p300-mediated p53 acetylation by complexing with these two proteins and that S1423/1524 phosphorylation is indispensable for this regulatory process. PP2Cδ, via dephosphorylation of ATM, suppresses DNA damage-induced BRCA1 phosphorylation, leading to inhibition of p300-mediated p53 acetylation. Furthermore, PP2Cδ levels correlate with histological grade and are inversely associated with BRCA1 phosphorylation and p53 acetylation in breast cancer specimens. C23, our newly developed PP2Cδ inhibitor, promotes the anticancer effect of doxorubicin in MCF-7 xenograft-bearing nude mice. Together, our data indicate that PP2Cδ impairs p53 acetylation and DNA damage response by compromising BRCA1 function.

18.
BMC Anesthesiol ; 19(1): 179, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601180

RESUMO

BACKGROUND: Delayed gastric emptying and the resultant "full stomach" is the most important risk factor for perioperative pulmonary aspiration. Using point-of-care gastric sonography, we aimed to investigate the prevalence of full stomach and its risk factors in elective surgical patients with type 2 diabetes. METHODS: Type 2 diabetic and non-diabetic elective surgical patients were included from July 2017 to April 2018 in a 1:1 ratio. The study was retrospectively registered at July 2017, after enrollment of the first participant. Gastric ultrasound was performed 2 h after ingesting clear fluid or 6 h after a light meal. Full stomach was defined by the presence of gastric content in both semi-recumbent and right lateral decubitus positions. For patients with full or intermediate stomach, consecutive ultrasound scan was performed until empty stomach was detected. Logistic regression analyses were used to identify risk factors associated with full stomach. RESULTS: Fifty-two type 2 diabetic and fifty non-diabetic patients were analyzed. The prevalence of full stomach was 48.1% (25/52) in diabetic patients, with 44.0% for 2-h fast after clear fluid and 51.9% for 6-h fast after a light meal, significantly higher than 8% (4/50) in non-diabetic patients (P = 0.000). The average time to empty stomach in diabetic patients was 146.50 ± 40.91 mins for clear liquid and 426.50 ± 45.25 mins for light meal, respectively. Further analysis indicated that presence of diabetes-related eye disease was an independent risk factor of full stomach in diabetic patients (OR = 4.83, P = 0.010). CONCLUSIONS: Almost half of type 2 diabetic patients have a full stomach following the current preoperative fasting guideline. Preoperative ultrasound assessment of gastric content in type 2 diabetic patients is suggested, especially for those with diabetes -related eye disease. TRIAL REGISTRATION: The trial was registered at www.clinicaltrials.gov with registration number NCT03217630 . Retrospectively registered on 14th July 2017.

19.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3502-3511, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602915

RESUMO

The aim of this paper was to compare the properties of Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets from dose-effect-toxicity on type Ⅱ collagen-induced arthritis( CIA) in rats. SD rats were randomly divided into eight groups,including normal group,model group,Tripterygium Glycosides Tablets groups( 1 times equivalent dose 0.009 g·kg-1,4 times equivalent dose 0.036 g·kg-1,16 times equivalent dose 0.144 g·kg-1),Tripterygium wilfordii Tablets groups( 1 times equivalent dose 0.007 5 mg·kg-1,4 times equivalent dose 0.030 mg·kg-1,16 times equivalent dose 0.120 mg·kg-1). Beginning on the first immunization,Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets administered intraperitoneally once a day. After the second immunization,the symptoms such as redness and swelling of joints were observed,and the clinical score and incidence of arthritis were evaluated. HE and Masson staining were used to examine the histopathological changes of joints. The expression level of anti-type Ⅱ collagen antibody Ig G in serum was detected by ELISA,routine testing of blood components,the concentration of ALP( alkaline phosphatase),ALT( alanine aminotransferase),AST( aspartate aminotransferase),GGT( gamma-glutamyltransferase),TBi L( total bilirubin),CRE( creatinine) and UREA( urea) in serum were detected by enzymatic assay. The rate of sperm deformity in the epididymis was evaluated under light microscope. The extent of damage to the testis and ovarian tissue was assessed by HE staining. Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets attenuated the inflammation,redness,swelling and deformity of joints and reduced the clinical score and incidence of arthritis in CIA rats. Meanwhile,it also exhibited obvious reduction in all pathological features such as joint synovitis,pannus,cartilage erosion and bone destruction. Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets reduced Ig G in a dose-dependent manner,and Tripterygium Glycosides Tablets is better than Tripterygium wilfordii Tablets( P<0.05 or P<0.01). The high doses of Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets could significantly increase the organ coefficient of liver and spleen and reduced RBC and HGB in CIA rats( P<0.01),and severity leading to death. Gastric mucosal injury and morphological changes of liver and kidney were not observed in CIA rats of Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets treatment group. The 4 and 16 times doses of Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets could significantly increase serum ALT,GGT and decrease CRE( P<0.05 or P<0.01). Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets could increase the sperm deformity rate and damage the testicular seminiferous tubules of CIA male rats. Severity increased with dose and time increasing. The effect of Tripterygium Glycosides Tablets( 16 times) is more significant than Tripterygium wilfordii Tablets( 16 times). Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets significantly delayed onset of arthritis and inhibited the paw edema and arthritic score. Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets also caused male reproductive damage,high dose affected hematopoiesis,and maximum dose leading to death. Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets all depended on dose-effect-toxicity manner. Anti-arthritis effect of Tripterygium Glycosides Tablets is better than Tripterygium wilfordii Tablets,but the toxicity of Tripterygium Glycosides Tablets maximum dose is more obvious. The relevant conclusions of our study will provide experimental references for clinical rational use of drugs,and further clinical studies are needed to confirm our conclusions.


Assuntos
Artrite Experimental/tratamento farmacológico , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/toxicidade , Glicosídeos/administração & dosagem , Glicosídeos/toxicidade , Tripterygium/toxicidade , Animais , Relação Dose-Resposta a Droga , Masculino , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Comprimidos
20.
Angew Chem Int Ed Engl ; 58(48): 17359-17364, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31595626

RESUMO

Harnessing metal-free photoinduced reversible-deactivation radical polymerization (photo-RDRP) in organic and aqueous phases, we report a synthetic approach to enzyme-responsive and pro-apoptotic peptide brush polymers. Thermolysin-responsive peptide-based polymeric amphiphiles assembled into spherical micellar nanoparticles that undergo a morphology transition to worm-like micelles upon enzyme-triggered cleavage of coronal peptide sidechains. Moreover, pro-apoptotic polypeptide brushes show enhanced cell uptake over individual peptide chains of the same sequence, resulting in a significant increase in cytotoxicity to cancer cells. Critically, increased grafting density of pro-apoptotic peptides on brush polymers correlates with increased uptake efficiency and concurrently, cytotoxicity. The mild synthetic conditions afforded by photo-RDRP, make it possible to access well-defined peptide-based polymer bioconjugate structures with tunable bioactivity.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA