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1.
J Nanosci Nanotechnol ; 20(3): 1845-1850, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492351

RESUMO

In this paper, we present a fast and green method to prepare Li0.44MnO2 nanorods by Li/Na ion exchange of Na0.44MnO2 templates. XRD and SEM confirm that the products still maintain the crystal and geometric structure of Na0.44MnO2 temples. Electrochemical tests show the capacity of Li0.44MnO2 nanorods is up to 218 mAh · g-1 at the current density of 0.1 A · g-1. Especially, the capacity of electrode is still located at 90 mAh · g-1 at the current density of 5.0 A· g-1 after cycling for 100 times. So Li0.44MnO2 nanorods have a potential application in the next generation of advanced batteries.

2.
Opt Lett ; 44(19): 4789-4792, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31568443

RESUMO

We report high Brillouin frequency shifts of 0.08±0.02 MHz/N in a 60 cm As2Se3-Poly(methyl methacrylate) (As2Se3-PMMA) hybrid microtaper with 2 µm As2Se3 core and 100 µm PMMA cladding diameters under a transverse load. Through finite element analysis of stimulated Brillouin scattering under the influence of contact stress from the loading fixture, such shifts are in accordance with a numerical value of 0.06±0.01 MHz/N. Further numerical analysis shows that uncertainties in the Brillouin frequency shift are the result of birefringence that is 27 times stronger than what is found in standard silica fibers.

3.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3391-3398, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602900

RESUMO

Tumors are major chronic diseases and seriously threaten human health all over the world. How to effectively control and cure tumors is one of the most pivotal problems in the medical field. At present,surgery,radiotherapy and chemotherapy are still the main treatment methods. However,the side effects of radiotherapy and chemotherapy cannot be underestimated. Therefore,it is of great practical significance to find new anti-cancer drugs with low toxicity,high efficiency and targeting to cancer cells. With the increasing incidence of tumor,the anti-tumor effect of traditional Chinese medicine has increasingly become a research hotspot. Triptolide,which is a natural diterpenoid active ingredient derived from of Tripterygium wilfordii,as one of the highly active components,has anti-inflammatory,immunosuppressive,anti-tumor and other multiple effects. A large number of studies have confirmed that it has good anti-tumor activity against various tumors in vivo and in vitro. It can play an anti-tumor role by inhibiting the proliferation of cancer cells,inducing apoptosis of cancer cells,inducing autophagy of cancer cells,blocking the cell cycle,inhibiting the migration,invasion and metastasis of cancer cells,reversing multidrug resistance,mediating tumor immunity and inhibiting angiogenesis. On the basis of literatures,this paper reviews the anti-tumor effect and mechanism of triptolide,and analyzes the current situation of triptolide combined with other chemotherapy drugs,in order to promote deep research and better clinical application about triptolide.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31591124

RESUMO

Recently, a novel plasmid-mediated tigecycline resistance mechanism, Tet(X4), has raised a global antimicrobial resistance concern (1, 2).….

5.
Medicine (Baltimore) ; 98(41): e17500, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31593118

RESUMO

Cerebrospinal fluid (CSF) protein values decline over the first few months of life as the infant's blood-CSF barrier matures. However, published studies have not reported CSF protein reference values of Chinese infants and differ in the reported rate, timing, and magnitude of this decline. The objective of this study was to determine reference intervals for CSF protein using available data of children in southern China. This retrospective study included infants who had a lumbar puncture (LP) performed in the Department of Pediatrics of Southern Medical University Affiliated Maternal and Child Health Hospital of Foshan of an urban tertiary care children's hospital between January 1, 2008 and May 31, 2018. Infants with conditions suspected or known to cause elevated CSF protein concentrations were excluded. Of 3712 infants undergoing LP, 1043 (28.1%) met inclusion criteria. Results showed that there is an age-related decline in CSF protein concentration. The median CSF protein value was 62 mg/dL [interquartile range (IQR): 47-81 mg/dL] in infants aged 0 to 56 days (group 1). The 95th percentile values were 116 mg/dL for infants 0 to 28 days and 80 mg/dL for infants 29 to 56 days. The 95th percentile values by age category were as follows: ages 0 to 14 days, 117 mg/dL; ages 15 to 28 days, 107 mg/dL; ages 29 to 42 days, 96 mg/dL; and ages 43 to 56 days, 74 mg/dL. The median CSF protein value was 21 mg/dL (IQR: 16-31 mg/dL) in infants aged 2 months to <3 years (group 2). The 95th percentile values were 57 mg/dL for infants 2 to <6 months and 34 mg/dL for infants 6 to ≤24 months. The 95th percentile values by age category were as follows: ages 2 to <3 months, 66 mg/dL; ages 3 to <4 months, 52 mg/dL; ages 4 to <5 months, 53 mg/dL; and ages 5 to <6 months, 42 mg/dL. We quantify the age-related decline in CSF protein concentrations among infants 2 years of age and younger and provide age-specific reference values. The values reported here can be used to interpret the results of LP in infants ≤2 years of age.

6.
Cancer Res Treat ; 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31588704

RESUMO

Purpose: Microtubule-associated protein 1 light chain 3 B (LC3B) serves as a key component of autophagy, which is associated with the progression of carcinoma. Yet, it is still unclear whether LC3B is also an independent risk factor for intrahepatic cholangiocarcinoma (ICC). We aim to explore the predictive value of LC3B on prognosis of ICC, and to establish a novel and available nomogram to predict relapse-free survival (RFS) and overall survival (OS) for these patients after curative-intent hepatectomy. Materials and Methods: From August 2004 to March 2017, 105 ICC patients were eligibly enrolled in the Third Affiliated Hospital of Sun Yat-sen University. Preoperative clinical information of enrolled patients was collected. Expression LC3B in the ICC specimen was detected by immunohistochemistry. Results: The 5-year RFS and OS in this cohort were 15.7% and 29.6%, respectively. On multivariate Cox regression analysis, independent risk factors for 5-year OS were cancer antigen 125, microvascular invasion, LC3B expression and lymph node metastasis. Except for the above 4 factors, neutrophil/lymphocyte ratio and tumor differentiation were independent factors for 5-year RFS. The area under the curve of nomograms for OS and RFS were 0.820 and 0.747, respectively. Conclusion: The nomograms based on LC3B can be considered as effective models to predict postoperative survival for ICC patients.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31584258

RESUMO

High-energy-density aqueous alkaline-ion batteries have drawn significant attention due to their intrinsic high security and low cost. As suitable anode materials with satisfying capacity and proper operating potential in aqueous electrolyte are limited, it is urgent to find candidate materials to build aqueous alkaline-ion batteries for practical applications. For the first time, highly crystalline NASICON -type phase TiNb(PO4)3 (TNPO) was explored as alkali ion intercalation host in aqueous battery technology. The TNPO material manifests high specific capacity of 94 mAh g-1 at 5C rate together with superior cyclic stability and remarkable rate capacity in aqueous electrolytes, which is assigned to its super stable three-dimensional (3D) tunnel structure. Furthermore, the aqueous lithium-ion battery based on TNPO anode and LiMn2O4 (LMO) cathode delivers a high specific energy of 121 wh kg-1 and a specific power of 189 W kg-1 with an output voltage of 2.1 V, while it exhibits excellent cycling performance (82% capacity retention after 1000 times at 5C rate). The remarkably overall performances of TNPO anode have clearly suggested that TNPO can be used as a promising electrode material of aqueous alkaline-ion batteries with high-energy-density for energy storage in future.

8.
Oncogene ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31576015

RESUMO

The molecular basis underlying the aggressive nature and excessive proliferation of cervical squamous cancer cell remains unclear. ΔNp63α is the predominant isotype of p63 expressed in the epithelia and regulates epithelial cell differentiation. The pro-/anti-tumor role of ΔNp63α in different kinds of solid tumors remains controversial and the precise molecular mechanisms are still elusive. In this study, we uncovered the molecular functions of ΔNp63α in cervical squamous cell carcinoma to clarify its roles as a tumor suppressor. We demonstrated that ΔNp63α suppressed cell migration, invasiveness, and tumor growth in SiHa and ME-180 cells with both in vivo and in vitro assays. Mechanistic investigation via RNA-sequencing and chromatin immunoprecipitation-sequencing revealed that ΔNp63α exerted its antitumor capacity via regulating the expression of a cohort of cell junction genes. Further, we showed that ZNF385B and CLDN1 were two direct ΔNp63α targets with significant relevance to cervical squamous cell carcinoma examined in cell cultures, tumor xenografts, and clinic tumors. We also demonstrated that ΔNp63α downregulated NFATC1 to reduce cisplatin resistance. These findings shed new lights on functions of ΔNp63α in tumors and providing novel insights in targeted therapy of cervical cancers.

9.
Public Underst Sci ; : 963662519877743, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31564207

RESUMO

By acknowledging that people are cognitive misers, this study proposes that people may rely on the illusion of knowing as cognitive devices for attitudinal or behavioral change, in addition to factual knowledge. Accordingly, this study shifted the focus of inquiry from assessing media effects in increasing factual knowledge to assessing how media consumption relates to the illusion of knowing. Using a nationally door-to-door survey in Singapore (N = 705), the results revealed that individuals' attention to media messages about climate change and elaboration of these messages were positively related to the illusion of knowing. Furthermore, elaboration had moderating effects on the relationship between media attention and the illusion of knowing. These findings suggest that media consumption of climate change messages could drive the illusion of knowing, which is speculated to account for pro-environmental behaviors in addressing climate change. Theoretical and practical implications were discussed.

10.
J Agric Food Chem ; 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31577435

RESUMO

Corn gluten hydrolysate (CGH) was prepared by food-grade bacterial proteases; alcalase and neutral protease. Digestion of CGH with carboxypeptidase A and leucine aminopeptidase extensively changed elution patterns of peptides from reversed phase high performance liquid chromatography-mass spectrometry (LC-MS), while digestion with pepsin and trypsin hardly affect elution patterns. Twenty-five major peptides in CGH were identified. After digestion with exopeptidases, only prolyl di-peptides and pyroglutamyl di- and tri-peptides remained, whereas the other 17 peptides completely disappeared. On the other hand, all 25 peptides remained after digestion with pepsin and trypsin. These facts suggest that majority of short chain peptides in food protein hydrolysates are degraded by exopeptidases during digestion and absorption processes. Thus, susceptibility to exopeptidases should be considered for prediction of bioactive peptide upon ingestion, which has not been considered in the most of previous studies on food-derived bioactive peptide.

11.
Cell Cycle ; : 1-13, 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31594449

RESUMO

Lung cancer is the leading cause of cancer-related deaths worldwide. However, tumor suppressor genes remain to be systemically determined for lung cancer. Here we report interferon regulatory factor 8 (IRF8), a member of the IRF family of transcription factors, as a potent lung tumor suppressor gene. Expression of IRF8 is frequently diminished in lung tumoral tissues and is associated with prognosis of non-small cell lung cancer (NSCLC) patients. Ectopic expression of IRF8 suppresses the NSCLC cells proliferation in vitro and tumorigenic potential in vivo. More importantly, forced expression of IRF8 through infection of recombinant virus inhibits lung tumorigenesis in genetically engineered mouse model (GEMM). Mechanistically, IRF8 inhibits AKT signaling and promotes accumulation of P27 protein, which results in senescence of lung cancer cells. Ectopic expression of IRF8 in tumor cells leads to regression of lung cancer tumor nodules in a xenograft tumor model. Our data, therefore, solidly shows IRF8 to be a lung cancer suppressor gene and may denote an opportunity for therapeutic intervention of NSCLC.

12.
Medicine (Baltimore) ; 98(37): e17150, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31517858

RESUMO

RATIONALE: Obturator dislocation of the femoral head combined with ipsilateral femoral neck and pubic fracture is a rare injury. We used cannulated screws combined with a femoral neck medial plate for the first time to treat this type of injury and achieved good follow-up results. PATIENT CONCERNS: The patient was hospitalized due to an accident resulting in swelling and deformity accompanied by limited mobility of the right hip and left knee. DIAGNOSES: X-ray examination and computed tomography confirmed that the patient suffered from right hip obturator dislocation, femoral head and neck fracture, pubic fracture, as well as open fracture of the left femoral shaft. INTERVENTIONS: Open reduction and internal fixation with cannulated screws and a medial support plate of the femoral neck were performed for the right hip obturator dislocation, femoral head and neck fracture, and the pubic fracture. Artificial bone grafting was performed to support the femoral head defect. Debridement and the second phase of internal fixation were executed to cure the open fracture of the left femoral shaft. OUTCOMES: The patient was followed-up for 6 months and showed good hip function. X-ray examination and computed tomography indicated that the fractures healed well without fracture displacement or loosening of the implants. Meanwhile, there were no signs of femoral neck valgus and femoral head necrosis observed. LESSONS: The combination of cannulated screws and medial support plate was suggested for the treatment of hip obturator dislocation and femoral head and neck fracture. Furthermore, partial weight loading exercise should be performed promptly to reduce the risk of muscular atrophy and myasthenia.


Assuntos
Fraturas do Colo Femoral/complicações , Fraturas do Colo Femoral/cirurgia , Luxação do Quadril/complicações , Luxação do Quadril/cirurgia , Ossos Pélvicos/lesões , Ossos Pélvicos/cirurgia , Adulto , Feminino , Fraturas do Colo Femoral/diagnóstico por imagem , Cabeça do Fêmur/diagnóstico por imagem , Cabeça do Fêmur/lesões , Cabeça do Fêmur/cirurgia , Fixação Interna de Fraturas , Luxação do Quadril/diagnóstico por imagem , Humanos , Ossos Pélvicos/diagnóstico por imagem
13.
Medicine (Baltimore) ; 98(37): e17162, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31517866

RESUMO

Vertebral compression fracture (VCF) is a common comorbidity of chronic obstructive pulmonary disease (COPD), and the coexistence of COPD and temporomandibular disorder (TMD) has been clinically noted. The present study aimed to investigate whether VCF increases the risk of TMD in patients with COPD.With a follow-up period of 15 years, this retrospective, population-based longitudinal cohort study enrolled sex- and age-matched COPD patients with and without VCF (1:3) who were identified from Taiwan's National Health Insurance Research Database from 2000 to 2015. Multivariate Cox regression analysis was performed to determine the risk of TMD in COPD patients with and without VCF. The cumulative risk of TMD between groups was estimated using Kaplan-Meier analysis.The risk factors for TMD in patients with COPD were VCF, osteoporosis, and winter season. The COPD with VCF group was more likely to develop TMD (adjusted hazard ratio = 3.011, P < .001) than the COPD without VCF group after adjustment for sex, age, variables, and comorbidities. In the subgroup analysis, the COPD with VCF group had a higher risk of TMD than the COPD without VCF group in almost all stratifications.COPD patients with VCF are at a higher risk of developing TMD. Clinicians taking care of patients with COPD should be aware of the occurrence of TMD as a comorbidity.


Assuntos
Fraturas por Compressão/complicações , Doença Pulmonar Obstrutiva Crônica/complicações , Fraturas da Coluna Vertebral/complicações , Transtornos da Articulação Temporomandibular/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Comorbidade , Feminino , Fraturas por Compressão/epidemiologia , Glucocorticoides/efeitos adversos , Glucocorticoides/uso terapêutico , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Prevalência , Doença Pulmonar Obstrutiva Crônica/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Fraturas da Coluna Vertebral/epidemiologia , Transtornos da Articulação Temporomandibular/complicações
14.
Nat Commun ; 10(1): 4387, 2019 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-31558724

RESUMO

Despite the importance of nanoparticle's multipods topology in multivalent-interactions enhanced nano-bio interactions, the precise manipulation of multipods surface topological structures is still a great challenge. Herein, the surface-kinetics mediated multi-site nucleation strategy is demonstrated for the fabrication of mesoporous multipods with precisely tunable surface topological structures. Tribulus-like tetra-pods Fe3O4@SiO2@RF&PMOs (RF = resorcinol-formaldehyde resin, PMO = periodic mesoporous organosilica) nanocomposites have successfully been fabricated with a centering core@shell Fe3O4@SiO2@RF nanoparticle, and four surrounding PMO nanocubes as pods. By manipulating the number of nucleation sites through mediating surface kinetics, a series of multipods mesoporous nanocomposites with precisely controllable surface topological structures are formed, including Janus with only one pod, nearly plane distributed dual-pods and tri-pods, three-dimensional tetrahedral structured tetra-pods, etc. The multipods topology endows the mesoporous nanocomposites enhanced bacteria adhesion ability. Particularly, the tribulus-like tetra-pods mesoporous nanoparticles show ~100% bacteria segregation and long-term inhibition over 90% after antibiotic loading.

15.
ACS Appl Mater Interfaces ; 11(40): 36506-36514, 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31514492

RESUMO

Using density functional theory calculations, we explored the potential of defective MoS2 sheets decorated with a series of single transition-metal (TM) atoms as electrocatalysts for the N2 reduction reaction (NRR). The computed reaction free-energy profiles reveal that the introduction of embedded single TM atoms significantly reduces the difficulty to break the N≡N triple bond and thus facilitates the activation of inert nitrogen. Onset potential close to -0.6 V could be achieved by anchoring various TMs, such as Sc, Ti, Cu, Hf, Pt, and Zr, and the formation of the second ammonia molecule limits the overall process. The Ti-decorated nanosheet possesses the lowest free-energy change of -0.63 eV for the potential determining step. To better predict the catalysis performance, we introduced a descriptor, φ, which is the product of the number of valence electron and electronegativity of the decorated TM. It shows a good linear relationship between the d-band center and binding energy of nitrogen, except for those metals with less than half-filled d-band. Although the metals in Group IIIB and IVB have strong adsorption interactions with N atoms, the Gibbs free-energy changes for desorption of the second ammonia are unexpectedly low. The selectivity of these systems toward nitrogen reduction reaction (NRR) is also significantly improved. Therefore, those defective MoS2 decorated with Sc, Ti, Zr, and Hf are suggested as promising electrocatalysts for NRR, for their both high efficiency and selectivity.

16.
ACS Appl Mater Interfaces ; 11(39): 35764-35769, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31508929

RESUMO

Industrial production of NH3 heavily depends on the conventional Haber-Bosch process under rigorous conditions with a large amount of energy consumption and carbon emissions. Electrocatalysis exhibits an intriguing prospect for the N2 reduction reaction (NRR) at ambient conditions. In this case, a high-efficiency and low-cost catalyst is paramount. In this letter, Cr3C2 nanoparticles and carbon nanofiber composite (Cr3C2@CNF) are proposed as a noble-metal-free NRR electrocatalyst for converting N2 to NH3 with an excellent selectivity. The optimal Faradic efficiency and NH3 yield rate achieved are as high as 8.6% and 23.9 µg h-1 mgcat.-1 at -0.3 V vs reversible hydrogen electrode in 0.1 M HCl, respectively. Theoretical calculations show a low reaction barrier of merely 0.53 eV in the enzymatic route for this catalyst.

17.
Acta Cir Bras ; 34(7): e201900710, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31531530

RESUMO

PURPOSE: To investigate the prognostic value of 17 platelet-based prognostic scores in patients with malignant hepatic tumors after TACE therapy. METHODS: In total, 92 patients were divided into death group and survival group according to long-term follow-up results. The AUC was calculated to determine the optimal cut-off values for predicting prognosis. To determine better prognostic models, platelet-based models were analyzed separately after being showed as binary according to cut-off values. Cumulative survival rates of malignant hepatic tumors were calculated using Kaplan-Meier curves and differences were analyzed by the log-rank test. Univariate and multivariate analyses were performed to identify platelet-based prognostic scores associated with overall survival. RESULTS: Univariate analysis showed that APGA, APRI, FIB-4, FibroQ, GUCI, King's score, Lok index, PAPAS, cirrhosis, number of tumors, vascular cancer embolus, AFP, ALP and APTT were significantly related to prognosis. A multivariate analysis showed that the APGA, number of tumors, ALP and APTT were independently associated with overall survival. CONCLUSION: This study showed that the APGA, a platelet-based prognostic score, was an independent marker of prognosis in patients with malignant hepatic tumors after TACE and was superior to the other platelet-based prognostic scores in terms of prognostic ability.


Assuntos
Aspartato Aminotransferases/sangue , Plaquetas/química , Carcinoma Hepatocelular/terapia , Quimioembolização Terapêutica/métodos , Neoplasias Hepáticas/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/sangue , Carcinoma Hepatocelular/sangue , Carcinoma Hepatocelular/mortalidade , Feminino , Humanos , Estimativa de Kaplan-Meier , Neoplasias Hepáticas/sangue , Neoplasias Hepáticas/mortalidade , Masculino , Pessoa de Meia-Idade , Contagem de Plaquetas , Prognóstico , Curva ROC , Estudos Retrospectivos
18.
ACS Appl Mater Interfaces ; 11(38): 34948-34956, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31475821

RESUMO

Energy storage of the lithium-ion hybrid capacitor can be upgraded through adjusting the mismatched rate qualities between the positive and negative electrodes because the positive electrode of the electrical double layer (EDL) stores and releases electricity in a smaller quantity, yet much faster than the negative battery electrode. To increase the EDL capacity, nitrogen-doped carbon (KPN900) with a hollow-onion structure is prepared with phenylphenol, achieving a surface area above 3000 m2 g-1. The capacitance of KPN900 displays a diffusive component of 57 F g-1, exceeding its capacitive counterpart at 10 mV s-1. Moreover, its total capacitance reaches 168 F g-1 at 1 mV s-1 with a diffusive component of 112 F g-1. On the other hand, the power of the negative electrode is improved through electrodeposition of metallic antimony on carbon nanotubes, Sb/CNTs, evidenced by the capacity of ∼250 mA h g-1 at 1.0 A g-1. Hence, the capacitor, with a 2:1 mass ratio of KPN900 to Sb/CNT, exhibits an effective trade-off between energy and power, distinct from the one-sided dependence on the carbon electrode of most hybrid capacitors. This capacitor stores 97 W h kg-1 at a power level 0.12 kW kg-1 and 17.4 W h kg-1 at power 7.90 kW kg-1.

19.
Nanoscale ; 11(37): 17230-17234, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31531482

RESUMO

A novel bioactive macromolecule based on morpholino-functionalized phosphorus dendrimers (generation 2, G2-Mor+) was developed for osteogenic differentiation of mesenchymal stem cells (MSCs). Interestingly, through in vitro tests, it was shown that G2-Mor+ dendrimer can strongly promote the transformation of MSCs into osteoblasts, which implies the potential application of phosphorus dendrimers in bone regeneration for precision regenerative medicine.

20.
Autophagy ; : 1-17, 2019 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-31500508

RESUMO

Synovitis is implicated in the pathology of osteoarthritis (OA) and significantly contributes to the development of OA. As a noninvasive physical therapy, low-intensity pulsed ultrasound (LIPUS) has been reported to possess anti-inflammatory effect in recent years. However, the role of LIPUS on synovitis of OA and the underlying mechanisms are little known. The present study showed that LIPUS ameliorated synovial inflammation in destabilization of the medial meniscus (DMM) mouse model and air pouch model, and alleviated pain gait patterns of DMM mouse. LIPUS dramatically inhibited the production of mature IL1B/IL-1ß (interleukin 1 beta) in vitro and in vivo. In addition, LIPUS upregulated the macroautophagy/autophagy level as well as accelerated the formation of an SQSTM1 (sequestosome1)-PKM (pyruvate kinase, muscle) complex in the lipopolysaccharide (LPS)-adenosine triphosphate (ATP)-treated macrophages. Besides, LIPUS downregulated the level of PKM2 in LPS-ATP-treated macrophages, which could be reversed by SQSTM1 knockdown. In brief, the present study for the first time demonstrates that LIPUS inhibits the production of mature IL1B partially via SQSTM1-dependent autophagic degradation of PKM2 in LPS-ATP-treated macrophages, which may further ameliorate the synovial inflammation and gait patterns in animal models. Our data provide new clues for the treatments of synovitis and other inflammatory diseases using LIPUS. Abbreviations: 3-MA: 3-methyladenene; ATG7: autophagy-related 7; ATP: adenosine triphosphate; BafA1: bafilomycin A1; BMDMs: bone marrow derived macrophages; CHX: cycloheximide; DMM: destabilization of the medial meniscus; ELISA: enzyme-linked immunosorbent assay; GFP: green fluorescent protein; IL1B/IL-1ß: interleukin 1 beta; LIPUS: low-intensity pulsed ultrasound; LIR: LC3-interacting region; LPS: lipopolysaccharide; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MDP: muramyl dipeptide; NFKB/NF-κB: nuclear factor kappa B; NLRP3: NLR family, pyrin domain containing 3; OA: osteoarthritis; PKM/PKM2: pyruvate kinase M1/2; PMA: phorbol-12-myristate-13-acetate; PYCARD/ASC; PYD and CARD domain containing; RFP: red fluorescent protein; siRNAs: small interfering RNAs; SQSTM1: sequestosome 1; TEM: transmission electron microscopy.

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